• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.171-177
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• 1992

The purpose of this study was to compare the test methods for predicting field emergence of Lotus corniculatus and Trifolium pratense. Four seed lots of two herbage legumes were controlled deteriorated, accelerated aged before germination test and conductivity of each seed lot was determined. Those germination percent and conductivity of seed lots were compared with actual field emergence rate. Field emergence rate could be estimated by the controlled deterioration test ($R^2$=0.687), by the accelerated aging test ($R^2$=0.260), and by the conductivity test ($R^2$=0.238). A multiple regression equation for predicting field emergence rate(equation omitted) was estimated as (equation omitted)=-198.16 ＋ 2.3493X$_1$-1.5564X$_2$ ＋ 1.9812X$_3$where X$_1$, percent germination of controlled deterioration test ; X$_2$, percent germination of accelerated aging test; and X$_3$, conductivity of solute leakage in the conductivity test. Conclusively, unless multiple tests are not available, the controlled deterioration test was comparatively high efficient for predicting field emergence rate, however, the combined measurements of those three tests can enhance the efficiency.

• Journal of Sericultural and Entomological Science
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• v.42 no.2
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• pp.67-72
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• 2000

Autotetraploid 'Sawonppong 26'was induced by dropping clochicine solution in growing point of Keomseolppong which is of high rootability and some agronomic charactelistics are as follows. Sawonppong 26 is a tetraploid of Keomseolppong with 56 of the chromosome number. Its fertlization percent was 59.4% and the germination percent of sank seed was normal. Sugar degree of mulberry fruit was 13.3% and total acidity was 0.69%. Single fruit weight of Sawonppong 26 was increased by 79%, as compared to that of Keomseolppong. Even though percent of survival of hard wood cutting was 94%, number of rootings was less and diameter of root was slender, it is expectedly a promising variety to be used for a breeding parent from the aspect of rootability.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.347-356
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• 2018

Chionanthus retusus is a small deciduous tree that is widely used in landscaping due to its beautiful white spring flowers and ornamental value. Conventional propagation through seeds requires one to two years of breaking dormancy. The objective of this study was to determine the conditions of in vitro germination in C. retusus. In vitro embryo culture was carried out to investigate the effects of six factors: basal media (McCown Woody Plant Medium (WPM) and Murashige and Skoog (MS)); plant growth regulators (different combinations and concentrations of naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BA), and gibberellic acid ($GA_3$)); embryo age (collected weekly beginning 36 days after fruit setting); low temperature pretreatment (storing $4^{\circ}C$ for 1, 2, 3, and 4 weeks); coconut additives (100, 200, and $300ml{\cdot}L^{-1}$); and genotype (grouping plants depending on their flowering nature). The basal medium used in this study was WPM with $2mg{\cdot}L^{-1-1}\;GA_3$, $20g{\cdot}L^{-1}$ sucrose, and $6g{\cdot}L^{-1}$ Agar. WPM medium mixed with $GA_3$, resulted in higher germination rate as compared to when using a combination of auxin and cytokinin. $GA_3$ at $2mg{\cdot}L^{-1}$ was the most effective of all combinations and concentrations of PGRs. WPM medium with $2mg{\cdot}L^{-1}GA_3$ resulted in better and faster germination (75.93%). Embryos collected at 57 days after fruit setting had the highest percent of germinated seeds (87.04%) while low-temperature pretreatment of fruits at $4^{\circ}C$ for two weeks produced the highest germination (95.37%). These results of this study could be an open ground for development of an efficient protocol for commercial production of the ornamental tree.

• The Korean Journal of Mycology
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• v.9 no.3
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• pp.141-145
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• 1981

Germination of the single-basidiospore of Agaricus bisporus, mycelial characteristics of the single spore culture and its fruit body formation were studied. Germination of the single basidiospore was the best on water agar. Of 6372 spores isolated, 1622 spore were germinated and the germination rate was 26 percent. Cultures from single basidiospores showed two distinct mycelial types on the compost extract sucrose agar: one was the strandy type and the other was the fluffy. The strandy type culture produced more sporophores than the fluffy. Of 778 strandy isolates screened, nine cultures yielded 5 percent higher than the origine. No. 1567 and 1708 were selected for the commercial cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.1
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• pp.61-66
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• 1987

Germination and seedling length of soybean seeds (cultivar: Williams) with and without polyethylene glycol-8000 (PEG) treatments were measured following preconditioning at two temperatures (15 and 30$^{\circ}C$) and two moisture levels 〔low (30 percent) and high (50 percent seed water content)〕 for 0, 2, 4, or 8 days. A split-split plot in time was used with four replications. Observations were made after two days of germination at 30$^{\circ}C$ Seedling growth accelerated with two days of preconditioning at 30$^{\circ}C$, but was reduced as preconditioning duration increased up to eight days at the same temperature. PEG treated preconditioned seeds exhibited reduced moisture uptake and seedling growth. Preconditioning at a high moisture level increased seedling moisture content and also increased seedling length until four days of preconditioning duration. Seedling dry weight decreased when preconditioning temperature was 30$^{\circ}C$ and when the high moisture level of preconditioning was continued for eight days.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.216-216
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• 2017

Primed seeds germinate faster and uniformly, since these have already started imbibition and metabolism requiring to complete germination. Several factors such as solution composition, osmotic potential, and treatment duration affect seed priming response. Water potentials of priming solution and germination response of primed seed were investigated to clarify the effects different inorganic salt types and the duration. Pepper seeds were primed in osmotic solutions that were osmotic potential ranged -1.54 to -0.45 MPa in an aerated solution of polyethylene glycol 8000 (PEG; 17%, 22%, 27%), and inorganic salt solution of $KNO_3$, $K_2SO_4$, and $Na_2SO_4$ (100, 200, 300mM) in aerated condition. The seeds were treated at $20^{\circ}C$ for 4, 6 day (D) and stepwise duration combined concentration of 100mM (2 or 4 days) and 300mM(2 or 4days). After soaking treatment, seeds were washed with distilled water and then were dried to approximately 6% moisture content in dry chamber at $25^{\circ}C$. The germination of seed was characterized by ISAT rules at $20^{\circ}C/30^{\circ}C$ and $15^{\circ}C$. Total germination percentage (GP), mean germination time (MGT), germination uniformity (GU), germination rate (GR), and health seedling percent (HS) were calculated on the germinated seed in a 14-day period. Seed water potential (${\psi}$) was correlated with water potential of priming solution ($r^2=0.84$). The effect of seed priming on germination varies with inorganic salt and organic agents (PEG). Germination percentage (GP, 77 and 73%), GR (21.3 and $19.4 %{\cdot}day^{-1}$), and HS (58 and 52 %) was greatest and lowest MGT (4.2 and 4.8 day) when they were primed in 100mM $K_2SO_4$ (${\psi}=-0.45MPa$), and 100mM $KNO_3$ (${\psi}=-0.52MPa$) for 6 days compared to untreated control (67% GP, $19.7%{\cdot}day^{-1}\;GR$, 18% HS, and 10.8 day MGT) in $15^{\circ}C$ chamber. Water potential less then -1.2MPa of osmotic solution resulted lower GP, GR and HS and in $K_2SO_4$ and $KNO_3$. However, stepwise treatment of water potential that changed solution as 100mM for 4 D + 300mM for 2 D or 300mM for 4 D + 100mM for 2 D) were not better than single concentration alone in germination characteristics.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.3
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• pp.183-189
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• 2004

This study was conducted to examine the changes in the seed physical characters and germinative properties of three tree species at three different storage times in order to determine the most suitable storage time and condition. Seed physical characters and germinative properties were examined from seeds of Fraxinus rhynchophylla, Zanthoxylum schinifolium and Staphylea bumalda that were stored at -18C for 1, 3 and 10 years, and the growth performance and physiological characters of their seedlings were analyzed. Seed physical characters and germinative properties showed significant differences between three storage times as well as three tree species. Seed moisture content of F rhynchophylla and S. bumalda and seed fresh weight of Z. schinifolium decreased with increase of storage time. Storage time represented negative correlation with moisture content and fresh weight (r = -0.822, P<0.01). Seed percent germination of Z. schinifolium and S. bumalda stored for 3 and 10 years decreased less than 15% and 10.7% respectively. Mean germination times for seed of F. rhynchophylla, Z. schinifolium and S. bumalda were delayed 11.9, 5.7 and 9.7 days after a storage time of 10 years, respectively. However the growth and viability of seedlings didn't show a special pattern for storage time and tree species.

• Korean Journal of Medicinal Crop Science
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• v.9 no.3
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• pp.198-204
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• 2001

$GA_3$ treatments for seeds of Perilla frutescens var. acuta Kudo were evaluated as a means of improving the percent germination, and the interactions of $GA_3$, mulching, and sowing depth on seedling emergence were investigated in late sowing. Percentage germination of seeds treated with different concentration of $GA_3$ was the most effective in 100 ppm treatment for 24 hours. The germinability according to the seeds soaking time was not significantly different over 12 hours in 100 ppm $GA_3$. At high temperature $(25^{\circ}C\;and\;30^{\circ}C)$, percentage germination was significantly increased in 100 ppm ${GA_3}$ treatment. In late sowing (1th or 15th May), seedling emergence was significantly higher in 100 ppm $GA_3$ treatment than nontreatment, and that was significantly increased when the seeds treated with 100 ppm $GA_3$ were mulched with 10 mm rice hull after molding with 5 mm soil depth.

• Journal of Sericultural and Entomological Science
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• v.44 no.1
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• pp.19-21
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• 2002

Suwonppong, which is the leading mulberry variety, has the fertility and the characteristics of fruit that are listed bellow. The percent of making seed is 61.8%, the percent of sank seed is 54.7% and the percent of germination is 95%. the percent of cross success is 31.8%. The single fruit weight of mulberry fruit is 3.58 g and its size is medium and big, the color of it is black. The sugar content of mulberry fruit is 15.7%(Brix), the total acidity is 0.18%, and the pH is 6.3. The maturing period of mulberry fruit is medium maturity and the degree of fruit setting is the same as Cheongilppong.

• Mycobiology
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• v.33 no.4
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• pp.206-209
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• 2005

Narceine methyl ester and narceine are potent alkaloids which were isolated from Corydalis longipes were found effective in vitro at very low concentration, i.e., $100{\sim}500\;ppm$ against spore germination of some test plant pathogenic fungi (Alternaria solani, A. tagetica, Cercospora abelmoschi, Curvularia maculans, Erysiphe cichoracearum, E. pisi, Fusarium udum, Helminthosporium oryzae, H. penniseti, Ustilago cynodontis). Among the test, phytopathogens the spores of F. udum, C. maculans and H. penniseti were highly sensitive at 200 ppm. However, spores of E. pisi, A. solani and A. tagetica were less sensitive at low concentration followed by other test fungi. Most of the fungi showed zero or nearly zero percent spore germination at 400 and 500 ppm.