• Title/Summary/Keyword: germinating seed

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Transplantation of Koelreuteria paniculata by Sea Current (모감주나무의 해류에 의한 전파)

  • 이영노
    • Journal of Plant Biology
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    • v.1 no.1
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    • pp.11-20
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    • 1958
  • I. Assumption: Mo-kam-ju, Koelreuteria paniculata is a woody plant mainly cistributed over Northern China. Its sporadic growth along the beaches of Korea and Japan is assumed to have been sowed by the seeds transported adrift on the current across the ocean. II. Use and Growth of the Plant: In China, this plant has been cultivated from early times mainly at temples, it seeds being used as rosaries, its flowers for yellow dyes and medicine for the eyes, and its leaves for black dyes. In Korea and Japan, these plants have been cultivated at temples and used as the material for rosaries. No natural growth of these plants was reported until 1919. III. Discoveries: In this domestic area, Dr. Chung Tae Hyun discovered the plant on the beach between Cho-Do and Chang-san-kot, Hwang-Hae Prov. in 1920. The reporter discovered them on the beach at Buk-Ni, Duk-jok-Do in 1948, at An-Hung in 1956 and on the beach at An-min-Do in 1957. In the Japan area, it was discovered for the first time twenty years ago, mainly along the coast line of the Japan Sea and some along the eastern coast line, at Subo, Yamaguchi Prefecture facing the Pacific Ocean. IV. Study and Experiment: A. Seed The seed coat is thick, non-permeable and floatable. A number of seeds were immersed in artificial sea water and fresh water separately. The seeds remained there for a hundred and forty-five (145) days from April 6, 1957 to August 29, 57. Thirty one seeds out of the fifty immersed in salt water and twenty seeds out of the fifty immersed in fresh water remained on the surface of the water, proving them to be non-permeable to both sea and fresh water. Of course, these had retained their germinating capabilities. Five (5) seeds out of twenty from the fresh water and six (6) out of thirty-one from the sea water were successfully germinated after a hole had been drilled in the seed coat and they had been planted. Thus their floating capability, non-permeability and germinating capability after a possible 145 day trip on the flowing currents has been proved satisfactory according to the assumption made above. B. Current As shown in the Data 2 and 3, the sea current initiated in the Pohai Sea flows westward down along the coast line of Korea reaching the southern part during the autumn and winter seasons. This fact also is in favor of the reporter's assumption. V. Discussion and Conclusion: The reporter concludes that, as discussed above, Koelreuteria paniculata which originates in Northern China is transported adrift on the flowing sea current to our western coast line, and also the coast lines of Shantung and Kuangtung of China and it is germinated on the sandy beaches forming new plants. Thus, the seeds drifted down on the southern beach of Korea and have been transported to the Japanese coast, adrift on the Tae-Ma current. Upon fruition, the seeds of the plants which settle on the coasts of western Korea and Japan will migrate to new places. It can be, however, assumed that while the thickness and nonpermeability of the coat enables the long travel in the water, this also can compose a difficulty in germination, consequently in developing a new distribution of this species.

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The Factors on Somatic Embryogenesis of Soybean [Glycine max. (L.) Merrill]

  • Kim, Kyong-Ho;Kim, Hag-Sin;Oh, Young-Jin;Suh, Sug-Kee;Kim, Tae-Soo;Park, Ho-Kee;Park, Moon-Soo;Kim, Seok-Dong;Yeo, Up-Dong
    • Journal of Plant Biotechnology
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    • v.2 no.3
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    • pp.123-128
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    • 2000
  • To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as material part and size of immature seed, 2,4-D, pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up to 22.9%) of somatic embryogenesis was obtained from the immature cotyledon, following embryonic axis and the immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25.4%) of embryogenesis was obtained from 3-4 mm length of the seed (after 25 days of flowering). The optimum concentration of 2,4-D for embryogenesis was 10 mg/L. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tips from the germinating somatic embryos, they were cultured on MS medium containing 2 mg/L indole-3-butyyic acid (IBA) and 1 mg/L 6-benzyladenine (BA). After then somatic embryos with one and three cotyledons were transferred to the growth regulator free medium. The medium exhibited the higher rate (ca. 50%) of development than the multiplication medium.

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The Arabidopsis Phytocystatin AtCYS5 Enhances Seed Germination and Seedling Growth under Heat Stress Conditions

  • Song, Chieun;Kim, Taeyoon;Chung, Woo Sik;Lim, Chae Oh
    • Molecules and Cells
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    • v.40 no.8
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    • pp.577-586
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    • 2017
  • Phytocystatins (PhyCYSs) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. Here, we characterized a PhyCYS from Arabidopsis thaliana, which was designated AtCYS5. RT-qPCR analysis showed that the expression of AtCYS5 in germinating seeds was induced by heat stress (HS) and exogenous abscisic acid (ABA) treatment. Analysis of the expression of the ${\beta}-glucuronidase$ reporter gene under the control of the AtCYS5 promoter showed that AtCYS5 expression during seed germination was induced by HS and ABA. Constitutive overexpression of AtCYS5 driven by the cauliflower mosaic virus 35S promoter led to enhanced HS tolerance in transgenic Arabidopsis, which was characterized by higher fresh weight and root length compared to wild-type (WT) and knockout (cys5) plants grown under HS conditions. The HS tolerance of AtCYS5-overexpressing transgenic plants was associated with increased insensitivity to exogenous ABA during both seed germination and post-germination compared to WT and cys5. Although no HS elements were identified in the 5'-flanking region of AtCYS5, canonical ABA-responsive elements (ABREs) were detected. AtCYS5 was upregulated in ABAtreated protoplasts transiently co-expressing this gene and genes encoding bZIP ABRE-binding factors (ABFs and AREB3). In the absence of ABA, ABF1 and ABF3 directly bound to the ABREs in the AtCYS5 promoter, which activated the transcription of this gene in the presence of ABA. These results suggest that an ABA-dependent pathway plays a positive role in the HS-responsive expression of AtCYS5 during seed germination and post-germination growth.

Isolation, Identification and Biological Control Activity of SKU-78 Strain against Ralstonia solanacearum (풋마름병균, Ralstonia solanacearum의 길항세균 SKU-78 균주의 분리 동정 및 특성)

  • Sung, Pil-Je;Shin, Jeong-Kun;Cho, Hong-Bum;Kim, Shin-Duk
    • Applied Biological Chemistry
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    • v.48 no.1
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    • pp.48-52
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    • 2005
  • Six stains of plant growth promoting rhizobacteria were selected through germinating seed assay and root colonization assay. Among them, SKU-78 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 60% reduction of bacterial wilt disease compared with the control. It was suggested that SKU-78 strain activated the host defense systems in plants, based on lack of direct antibiosis against pathogen. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, SKU-78 stain was identified as Bacillus sp. SKU-78.

Studies on the Germination Promotion of the Zoysia japonica seeds. (한국잔디(Zoysia japonica Steud.) 종자의 발아촉진에 관한 연구)

  • 전우방
    • Asian Journal of Turfgrass Science
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    • v.1 no.1
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    • pp.30-36
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    • 1987
  • In order to promote the germination of Korean lawn grass(Zoysia japonica Steud.) seeds a gravimetric seed selection with a mixed chemical solution and the germination promotion with KOH treatment were studied and following results were obtained. 1. When immersed into methanol(S.G., 0.7) 14 percent of immature seeds were precipitated. 2. With water (S.G., 1.0) 36.5 percent of mature seeds were submerged. 3. On a mixture of carbon tetrachloride (S.G., 1.6) and ethylether (S.G., 0.7) in a ratio of 4:6 the seed selection was most effective. 4. When treated with KOH solution the germination rate was 71.01 percent in contrast to 6.0 percent of germination without the treatment. 5. As for concentration of KQH solution, 15 percent depressed the germination (46.7 %) whereas above 20 % the germination rate was gradually increased (79.32 %) up to 40 percent of concentration where the concentration started to depress germination ratio (77.32 %). 6. Finally, in connection with the duration of KOH treatment, a time lapse of up to 20 minutes depressed the germinating ratio(70.02 %) and 30 minutes to 45 minutes increased this ratio to 94.52 percent. 60 minutes of treatment depressed the germination to 75.62 percent.

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Plant Growth Regulator Produced by Streptomyces sp. (Part II) Conditions of Production and Some Properties of the Plant Growth Regulator

  • 김광현;서정훈
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1978.10a
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    • pp.207.5-208
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    • 1978
  • Effects of the plant growth regulator (P. G. R.)on the reaction of proteinase, $\gamma-amylase$ and acid phosphatase were investigated, and also were the conditions of production of P. G. R. by Stroptomyces sp. 445. The P. G. R. had no effect on the act ivities of such enzymes in mung bean seedling. But in germinating seed previously treated with P. G. R. it effected the activity of protease in cotyledon. In the conditions of production of P. G. R., the maxim, activity was appeared in shaking cutlure at $30^{\circ}C$ for 5 days, and by the addition of peptone or casein hydrolysate as nitrogen source, soluble starch as carbon source, and sulfur as metal ion.

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Agronomic Practces on Intensive Pasture Establishment (집약초지의 조성기술)

  • 김동암;조무환
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.2 no.2
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    • pp.1-15
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    • 1981
  • In reviewing of the literature concerning agronomic practices on intensive pasture establishment the following suggestions were made : (1) The seedbed preparation should be involved physical manipulation of the soil tilth and consolidation of the soil by harrows and heavy roller. (2) In terms of methods of sowing, drilling favours the first and broadcasting the second however, the apparent superiority of band sowing over the above conventional methods is to reduce the direct toxic chemical effects from fertilizers to germinating seeds. (3) The most satisfactory seeding depth for grasses and legumes is commonly agreed to be approximately 1.3cm and seeding depth greater than 2.5cm seems to be generally detrimental. (4) Autumn and spring are most common sowing periods for grasses and legumes, however, early autumn being the most generally favoured, particularly in areas not subject to severe winter kill. (5) Select nurse crop species or cultivars which make Bess demands on light, nutrients and water, and lower seed rates should be used. (6) Seeding mixtures should be simple rather than complex and aggressive species should be included to a minor extent.

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Zinc Chloride Toxicity on Free Proline and Organic Acids in Germinating Rice Seed

  • Kim, Sang-Kuk;Chung, Sang-Hwan;Lee, Sang-Chul;Lee, Seong-Phil
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.163-165
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    • 1999
  • The study was conducted to find the critical concentrations of zinc toxicity and to determine the changes of the contents of free proline and organic acids with treatment of different zinc chloride concentrations during rice germination and seedlings grown for seven days. The concentration of zinc chloride, 140 ppm, inhibited root elongation as much as 46 times compared with the control, and the germination rate was also decreased in all treatments of zinc chloride, showing that the germination rate decreased more with increasing concentrations of zinc chloride. Its rate was only 13% with treatment of 140 ppm zinc chloride. The content of free proline with treatment of zinc chloride, 140 ppm, was highest about 4,873 $\mu$M at 3 days compared with the control. Malic acid concentration with treatment of zinc chloride, 140 ppm, increased to approximately 4 times compared to the control. Citric and succinic acid content were also slightly increased in all treatments of zinc chloride.

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Development of genetic transformation method of Korean soybean (국내콩 형질전환 기술개발)

  • Jeon, Eun-Hee;Chung, Young-Soo
    • Journal of Plant Biotechnology
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    • v.36 no.4
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    • pp.344-351
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    • 2009
  • Current status of soybean transformation method in Korera was reviewed with recent publications. Most frequently used method for genetic transformation was Agrobacterium-mediated transformation on cotyledonary node which is most popular method used in foreign country. In addition to this, various methods such as sonicationmediated transformation, in planta transformation, and transformation on meristem tissue of germinating seed, have been tried in Korea, even though their efficiencies on repeatability and stability were relatively low. Based on the promising results developed recently by reviewer, several important considerations for successful soybean transformations were suggested. They are 1) proper genotype screening, 2) targeting transformation on exact point, 3) multiple shoot formation, 4) efficient selection pressure, 5) successful shoot elongation, 6) efficient root formation. These are the basic requirements for stable and highly efficient soybean transformation of Korean soybean.

Cloning, Characterization, and Functional Analysis of Maize DEHYDRIN2

  • Paek, Nam-Chon;Jung, Hun-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.2
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    • pp.116-122
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    • 2002
  • Dehydrins (LEA Dll proteins) are one of the typical families of plant proteins that accumulate in response to dehydration, cold stress, abscisic acid, or during seed maturation. A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress. The deduced protein sequence indicated a dehydrin gene encoding SK$_3$ LEA protein typically expressed during cold acclimation, but not by drought stress in barley and wheat. Thus, it was named maize DEHYDRIN2 (ZmDhn2). It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage, but not under cold stress. ZmDhn2 gene was transformed into Arabidopsis thaliana for functional analysis under drought condition. From electrolyte leakage test, no significant difference showed between wild type and transformants under normal growth condition, but the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress. It suggests that ZmDHN2 playa role in increasing drought tolerance.