• Title/Summary/Keyword: germinating seed

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Screening Method for Excessive Water Tolerance at Germinating Stage of Peanut (땅콩 발아기의 내습성 검정 방법)

  • Pae, Suk-Bok;Hwang, Chung-Dong;Lee, Myoung-Hee;Kim, Sung-Up;Oh, Ki-Won;Lee, Byung-Kyu;Baek, In-Youl
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.4
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    • pp.439-442
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    • 2013
  • This study was conducted to develop the testing method for excessive water tolerance at germinating stage and to screen 31 domestic peanut cultivars. Regardless of peanut grain scales, the amount of seed absorption nearly reached the peak in 10 hours after imbibition. When peanut seed in vermiculate soil was directly soaked in water, ability of emergence did not reduced until 16 days and then sharply reduce to 25 days with non-emergence. When seeds germinated for 1, 2, 3, and 4 days after seeding (DAS) were soaked, the emergence abilities were distinctively varied according to the sequent soaking days such as 1, 2, 3, 4, and 5 days. This explained the negative relationship between first germinating days (DAS) and following soaking days. Using the method of 2 day germinating and 3 day soaking that show less than 70% of emergence ability, 31 peanut cultivars were applied to test excessive water tolerance. Emergence rates varied 0% to 69% according to cultivar. Cultivar Daekwang, Sinkwang, Daecheong and Baekseon had over 50% emergence rates. These results suggested that the degree of water resistance in germinating stage may be important point to evaluate the excessive water resistance among cultivars.

Seed Pelletizing of Salvia splendens and Calendula officinalis for the Greening and Re-vegetating (녹화와 식생조성을 위한 샐비어와 금잔화 종자의 펠렛처리)

  • Kim, Seung-Hyun;Lee, Byoung-Ryong;Choi, Byoung-Kon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.5
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    • pp.67-75
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    • 2007
  • The purpose of this study was to pelletize to calendula and salvia seeds with peat moss as basic material for the efficient greening and vegetating of slopes and damaged areas. Also the pelletizing was compressed by spherical types that mixed basic fertilizer of N.;300 mg/l, P.;200 mg/l, K.;400 mg/g and plant growth regulator of A and NAA each 300PPM. Soil and soil surface seeding methods were researched to find the growing state of germination percent, germination date, germination force, length of leaf, number of leaf, width of leaf, length of plant, and etc. Comparing with controlled pelletizing, peat moss and +GA pelletizing treatments resulted in higher from two to three times as following growing states : length of leaves, number of leaves, width of leaves, length of plants, length of roots, fresh weights, and ratio of germination. Especially the two treatments above showed four more times effects than the +NNA treatment. Also their germinating date germinating force were earlier and stronger. The surface seeding method was superior to soil seeding.

A Study on Amylase Activities During Germination of Maize Seeds (옥수수의 발아과정중(發芽過程中) AMYLASE활성(活性))

  • Choi, Kook-Chi
    • Applied Biological Chemistry
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    • v.27 no.2
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    • pp.107-111
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    • 1984
  • Changes of starch, soluble sugar and activities of amylase in germinating maize seeds were studied with comparison of germinating barley seeds. The starch in the endosperm was degraded slowly during the course of germination, and starch degradation of maize seeds compared with barley seed was found after 2 or 4 days of seed germination. There was a transient decline of soluble sugar come-its in ungerminated seed at early stages, followed by the rapid increase which concided with the degradation of starch. Activities of amylases were increased with the progress of germination, and maximum activities were founded after 8 days of seed germination. The higher activities of ${\alpha}$- and ${\beta}$-amylase were found at Hwang-ok maize and barley, respectively.

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Analysis of Seed Germinating Vigor, Germination Speed and Peak Time in 9 Fescues under Alternative and Natural Conditions at the Room Temperature (자연 실온 및 변온 환경에서 광엽 및 세엽 훼스큐 9종류의 종자 발아력, 발아세 및 발아 피크 기간 분석)

  • Kim, Kyoung-Nam
    • Weed & Turfgrass Science
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    • v.6 no.3
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    • pp.235-248
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    • 2017
  • This study was initiated to investigate seed germinating vigor, germination speed, and establishment characteristics of Festuca species under natural conditions ($5-25^{\circ}C$). Germination rate was 62.0% in CRF 'Audubon' to 97.0% in TF 'Olympic Gold'. It was greater with TF over fine-type fescues, being TF>SF>CF, HF>CRF. The germination speed, measured as days to seed germination of 50% through 80%, was fastest with TF, medium to high with CF, medium with HF and SF, and slowest with CRF. However, it was approximately 4 days slower under natural conditions, as compared with that under alternative conditions ($15-25^{\circ}C$). Germination peak time was much faster with TF. It ranged from 0.73 to 1.84 days with TF, being 1.45 days in average. But it varied with 2.16 to 11.75 days among fine-type species. The peak time was longer in order of SFCF>SF>HF>CRF.

Characteristics of Seed Structure and Seedling Development in Polygonatum odoratum Druce (둥굴레의 종자(種子) 구조(構造)와 출아(出芽) 특성(特性))

  • Kang, Jin-Ho;Kim, Dong-Il;Ryu, Yeong-Seop;Bae, Kee-Soo;Han, Kyung-Soo
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.2
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    • pp.102-107
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    • 1998
  • Solomon's seal (Polygonatum odoratum) seedlings raised through its seeds can replace the rhizomes impelling higher cost for transplanting, This experiment was done to determine the seed characteristics and the germinating processes to give some information on bulk production of seedlings using the seeds. The external or internal morphology of seeds or seedlings grown in lab. or greenhouse was examined mainly with stereomicroscope. The external shape of Solomon's seal seed was hard seed-coat and orthotropous ovule with linear type embryo stretching to the center of seed. Germination proceeded through the several steps. The lower part of seed embryo having the primordia of bulbil and roots first grew before the bulbil and roots was developed from the primordia. The lower part of embryo was enlarged toward the endosperm of seed as soon as seed germinated. Then epicotyl was formed on the apex of bulbil. The epicotyl was elongated after at least 6-week chilling treatment for breaking its dormancy and the first leaf shape was affected by light intensity given during seedling emergence. The bulbil was the first organ of the rhizome used as tea or herb medicine.

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Analysis of germinating seed stage expressed sequence tags in Oryza sativa L. (벼 발아종자 발현유전자의 발현특성분석)

  • Yoon, Ung-Han;Lee, Gang-Seob;Kim, Chang-Kug;Lee, Jung-Sook;Hahn, Jang-Ho;Yun, Doh-Won;Ji, Hyeon-So;Lee, Tae-Ho;Lee, Jeong-Hwa;Park, Sung-Han;Kim, Gun-Wook;Seo, Mi-Suk;Kim, Yong-Hwan
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.281-288
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    • 2009
  • Seed germination is the important stage to express many genes for regulation of energy metabolism, starch degradation and cell division from seed dormancy state. For the functional analysis of seed germination mechanisms, we were analyzed the rice cDNA clones (Oryzasativa cultivar Ilpum) obtained from seed imbibition during 48 hours. Total number of 18,101 Expressed Sequence Tags (ESTs) were clustered using SeqMan program. Among them, 8,836 clones were identified as unique clones. We identified the chitinase gene specifically expressed in seed germination and amylase gene involved to starch degradation from the full length cDNA analysis, and several genes were registered to NCBI GeneBank. To analyzed the commonly expressed genes between inmature seed and germinated seed, 25,66 inmature ESTs and 18,101 germinated ESTs were clustered using SeqMan program and identified 2,514 clones as commonly expressed unigene. Among them, alpha-glubulin and alcohol dehydrogenase I were supposed to LEA genes only expressed in the immature and germinated seed stages. For the clustering of orthologous group genes, we further analyzed the 8,836 EST clones from germinating seeds using NCBI clusters of orthologous groups database. Among the clones, 5,076 clones were categorized into information storage and processing, cellular processes and signaling, metabolism and poorly characterized genes, proportioning 783 (14.29%), 1,484 (27%), 1,363 (24.8%) and 1,869 (34%) clones to the previous four categories, respectively.

Collection, Conservation, and Utilization of Plant Genetic Resources -emphasis on weeds- (유용식물 유전자원의 수집 보존 및 이용에 관한 연구 -잡초를 대상으로-)

  • Kim, Kil Ung
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.9-18
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    • 1990
  • Germinating ability of 54 weed species collected throughout Korea in 1987 and 1988 was determined in incubator at $25^{\circ}C$ for 15 days. The seeds of Echinochloa sp, collected from 70 different places throughout Korea, were classified by Yabuno's seed morphology method. Plant characters of two identified Echinochloa species, one having two subspecies were evaluated. SDS-PAGE(sodium dodecyl sulfate polyacrylamide gel electrophoresis) of seed protein assay was used to identify locally collected Echinochloa species classified by Yabuno's seed morphology method. 1. Out of 54 weed species collected, 31 species showed germination at 15 days after incubation, and 14 species showed over 50% and no germination was observed in 24 species, indicating that approximately 40 species had varied degree of germinating ability. 2. It was confirmed that two annual species of Echinochloa such as E. oryzicola and E. crus-galli occurred in Korea and one of which particularly E. crus-galli has two subspecies such as E. c. var. praticola and E. c. var. crus-galli. 3. E. oryzicola was distinguished from E. c. var crus-galli and E. c. var praticola by presence or absence of one major protein band in B zone. However, it was rather difficult to distinguish E. crus-galli species because they showed similar protein pattern.

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Direct Seeding Cultivation on Submerged Paddy in Rice II. Dissolved Oxygen Uptake and Germination Properties of Rice Varieties in the $O_2$ Saturated Water (벼 담수토중 직파 재배 연구 II. 벼 품종의 수중발아시 용존산소 흡수와 발아특성)

  • Lee, Chul-Won;Seong, Ki-Yeong;Park, Seok-Hong;Park, Rae-Kyeong;Cho, Dong-Sam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.1
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    • pp.97-101
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    • 1988
  • The objective of this paper was to examine the oxygen requriement of rice seeds when the seeds were germinating under the water. The trial was carried out in the laboratory with the different water temperature (25$^{\circ}C$, 17$^{\circ}C$) in the Crop Experiment Station, Suwon. The tested varieties were 6 in japonica and 6 in indica (I) ${\times}$ japonica (J). In the 25$^{\circ}C$ water temperature rice seeds absorbed more dissolved oxygen (DO) and germinating duration was shorter than in the 17$^{\circ}C$ water temperature. DO uptake of japonica rice seeds was faster than that of indica ${\times}$ japonica rice seeds, and the germinating ratio of japonica rice seeds was higher than that of I${\times}$J rice seeds in the water. DO requirements of germinating rice seeds in the water were average 82.4 ${\mu}$g per seed up to coleoptile appearance and average 123.6 ${\mu}$g per seed up to appearance of radicle in the japonica varieties, but I${\times}$J varieties were 96.9 ${\mu}$g and 145.1 ${\mu}$g respectively. Especially when the rice seeds were germinated in the water, length of coleoptile and radicle of japonica rice were significantly longer than those of I${\times}$J rice varieties.

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Comparison of Seed Germinating Vigor, Early Germination Speed and Germination Peak Time in Perennial Ryegrass Cultivars under Different Germination Conditions (발아환경에 따른 퍼레니얼 라이그래스 품종간 발아력, 기발아속도 및 발아피크기간 비교)

  • Kim, Kyoung-Nam
    • Weed & Turfgrass Science
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    • v.2 no.3
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    • pp.274-282
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    • 2013
  • Research was initiated to investigate early germination and establishment characteristics of perennial ryegrass (PR, Lolium perenne L.). An alternative condition was applied in Experiment I with 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA condition). Experiment II was conducted under $5^{\circ}C$ to $25^{\circ}C$ (natural condition). Significant differences were observed in germination percentage, germination speed and germination peak time. Seed germinating vigor was 66.00 to 93.75% under ISTA and 66.25 to 93.50% under natural conditions. Germination speed, measured as days to germination of 50% through 90%, was much faster with 'Pennant II', 'Brightstar II', 'Sonata' and 'Accent II' under ISTA conditions. But it was faster only with 'Pennant II' and 'Brightstar II' under natural conditions. Germination peak time ranged 1.48 to 5.65 days under ISTA, while 2.17 to 10.63 days under natural conditions. Regardless of growing conditions, the shortest cultivars in peak time were 'Sonata', 'Pennant II', and 'Brightstar II' and the longest one 'Revenge GLX'. Considering germinating vigor, early germination speed and germination peak time, 'Pennant II', 'Brightstar II', 'Sonata' and 'Accent II' were regarded as excellent cultivars under ISTA conditions, while 'Pennant II' and 'Brightstar II' under natural conditions.