• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1227-1231
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• 2002

A powerful tool for chicken transgenesis could be established by employing a germline chimera production through primordial germ cell transplantation. This study was conducted to examine whether foreign gene-transfected gonadal primordial germ cells (gPGCs) have a migration activity into the gonad after transfer to recipient embryos. In Experiment 1, gPGCs of Korean Ogol Chicken were retrieved from 5.5-day-old embryos and subsequently transferred to the dorsal aorta of 2.5-day-old White Leghorn embryos after being labeled with PKH26 fluorescent dye. To confirm migration activity after transplantation, recipient embryos were sacrificed and examined on 3 days after transfer. Sex determination was concomitantly undertaken to examine whether sex of recipient embryos could affect the migration activity of gPGCs. All of embryonic gonads examined showed positive signals with PKH26 fluorescence and W-chromosome specific band by polymerase chain reaction (PCR) was detected in male embryos when gPGCs with ZW chromosome were transferred to recipient embryos. In Experiment 2, retrieved gPGCs were transfected with LacZ gene-containing cytomegalovirus promoter ($pCMV{\beta}$) by electroporation and subsequently transferred to recipient embryos. LacZ gene expression was identified in the gonads of 6 or 10-day-old recipient embryos and hatched-chicks. A total of 20 embryos and 12 hatched-chicks were examined and 11 of them (10 embryos and one hatched chicken; 11/32=34.4%) expressed $\beta$-galactosidase, a marker substance of LacZ gene. The results of this study demonstrated that foreign gene-transfected gPGCs can migrate and settle down into the gonad after being transferred into the blood vessel of the recipient embryos. This established technique will contribute to developing a peer biotechnology for transgenic chicken.

• Journal of Yeungnam Medical Science
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• 제2권1호
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• pp.293-297
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• 1985

저자들은 최근 50세 남자 환자에서 병리조직학적으로 증명된 정상피종과 태아성 암으로 구성된 결합종양 1예를 경험하였기에 문헌 고찰과 아울러 보고하는 바이다.

• 한국동물학회지
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• 제21권2호
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• pp.43-58
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• 1978

양서류 수정란을 제 1 난할전에 중금속으로 처리하였던바 개구리의 시원생식세포수의 양전변화를 가져 왔다. 중금속의 일정량의 처리는 양서류 초기발생중에 시원생식세포 형성을 저하시켰다. 납 70ppm과 카드뮴 4ppm 이상에서 배양하면 9내지 12mm배에서 시원생식세포의 수적감소를 가져 왔으나 그후의 발생에서는 거의 정상군과같은 증가율을 보였다. 한편 수은 0.8ppm이상의 처리군에서는 생식 세포형성에 보다 심한 저하를 가져 왔으며 그후의 증가율도 대체로 낮았다. 위의 사실들은 자외선 조사 실험에서 얻은 결과와는 달리 양서류란에 있어서 중금속 처리는 조직에 심한 손상을 주는 다량투여에도 시원생식세포의 완전 배제에는 효과가 적다는 것을 암시한다.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2002년도 가을 학술발표논문집
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• pp.98-99
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• 2002

Collection of large number of gonadal Primordial germ cells(gPGCs) is a prerequisite factor for improving germline transmission efficacy in the aves, In this study a magnetic-activated cell sorter(MACS) was applied for improving retrieval efficacy of quail gPGCs and the migration capacity of MACS-separated gPGCs was further examined after being transplanted to recipient embryos. We also induced germline chimeras by transfer of MACS-separated quail gPGCs at the efficiency of 17.4% on average.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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• pp.69-70
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• 2001

Lectins have great potential as to determine the alternation of the distribution of cell surface carbohydrates during cellular development and differentiation. Here, we investigated the presence and distribution of cell surface carbohydrates on chicken primordial germ cells (PGCs) during the migration and gonadal stages using a variety of lectins. A total of six FITC-labelled lectins from several specificity classes were used: ConA (glucose/mannose), WGA (N-acetylglucosamine), STA (N-acetylglucosamine), DBA (N-acetylgalactosamine/galactose), UEA-I (fucose) and PHA-E (oilgosaccharide). As a results, PGC-specific binding was observed in STA. PGCs of migration stage (2.5- and 5.5-day embyos) were STA-positive whereas PGCs of 10-day embryonic gonad were not. The results suggest that N-acetylglucosamine residuse are present specifically in migrating chicken PGCs and changes during development.

• 대한치과의사협회지
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• 제16권3호통권106호
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• pp.193-195
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• 1978

The author observed the effect of x-ray irradiation on the tooth germ development of the rat fetuses. The lower right abdomen of the pregnant rats were exposed to x-ray irradiation (400 rads) on 9½th day of qestation. At 18½th day of qestation, the fetuses were removed from their mothers and histological sections of molar region were prepared. The results were as folows: 1. In the experimental fetuses, no significant changes appeared in the histological aspects of the enamel pulp, except the poor development of the innerenamel epithelium in the cusp region. 2. Pulp cells of cusp region in the irradiated fetuses were not differentiated to odontoblasts, The arrangement and population of pulp cells showed marked regional differences in the dental papilla. 3. Developmental features of dental follicle of irradiated fetuses were similar with controls.

• Clinical and Experimental Reproductive Medicine
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• 제50권4호
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• pp.213-222
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• 2023

Cryopreservation is an option for the preservation of pre- or post-pubertal female or male fertility. This technique not only is beneficial for human clinical applications, but also plays a crucial role in the breeding of livestock and endangered species. Unfortunately, frozen germ cells, including oocytes, sperm, embryos, and spermatogonial stem cells, are subject to cryoinjury. As a result, various cryoprotective agents and freezing techniques have been developed to mitigate this damage. Despite extensive research aimed at reducing apoptotic cell death during freezing, a low survival rate and impaired cell function are still observed after freeze-thawing. In recent decades, several cell death pathways other than apoptosis have been identified. However, the relationship between these pathways and cryoinjury is not yet fully understood, although necroptosis and autophagy appear to be linked to cryoinjury. Therefore, gaining a deeper understanding of the molecular mechanisms of cryoinjury could aid in the development of new strategies to enhance the effectiveness of the freezing of reproductive tissues. In this review, we focus on the pathways through which cryoinjury leads to cell death and propose novel approaches to enhance freezing efficacy based on signaling molecules.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.22-23
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• 2003

Primordial germ cell (PGC) is the progenitor cell of the germ cell lineage and eventually give rise to gametes that are responsible for creating individual organisms via a fertilization process. This means that PGC is a unique cell that can be converted into individual fish. This advantage of PGCs would make it possible to develop various applications in the field of fish bioengineering. First, PGCs may make it easier to preserve the genetic resources of fish. Cryopreservation of fish eggs or embryos has not been successfully achieved so far. Therefore, the only possible method to preserve genetic resources of fishes is to raise fish as live individuals. If PGCs isolated from various fishes could be cryopresewed, these cells could be converted into live fishes via germ-line chimera production. This is particularly useful for preserving genetic materials of endangered species. Even if the species of interest were to become extinct, it could be recovered by the transplantation of cryopreserved PGCs into the embryos of a closely related species. Another application of this technology is in what could be termed "surrogate broodstock technology". (중략)

• Journal of Animal Science and Technology
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• 제55권5호
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• pp.417-425
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• 2013

동결 닭 PGCs의 생식계열 키메라를 이용한 생체에의 복원을 실용화 하기 위해서는, 닭 PGCs의 동결보존기술의 향상에 의해 동결 및 융해 후의 많은 생존세포를 확보 하는 것과, 생식계열 키메라의 제작효율을 높이는 것이 반드시 필요하다. 닭 PGCs는 배양 5.5일령의 닭 원시생식선으로부터 채취하고, ACS 방법에 의해서 순수 닭 PGCs를 분리했다. 닭 PGCs의 동결보존실험결과 다음의 4종류의 동결방법을 각각 비교 검토했다. 1. 플라스틱 스트로에 의한 완만동결법 (SF), 동결보호물질은 2M 에틸렌 글리콜 (EG), 2. 스트로에 의한 급속동결법 (RF), 8M EG + 7% PVP, 3. 동결용 Cryotube에 의한 SF, 2M EG, 4. 튜브에 의한 SF, 10% DMSO. 동결 및 융해 후의 PGCs의 생존율은 각각 76.4%, 70.6%, 80.5%, 78.1%로 관찰되었다.

• 한국식품영양학회지
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• 제15권2호
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• pp.151-157
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• 2002

대두 배아로부터 saponin을 분리.정제하여 유리기 억제효과 및 동물의 암세포에 대한 세포 독성을 조사하였다. 유리기 억제활성에 대한결과를 보면 대두 배아 saponin을 0.5 및 1.0%씩 첨가한 급여 식에서 4주 동안 사육한 후 간장을 절취하여 간장 획분의 활성산소 및 항산화 관련 효소에 대하여 평가하였다. 간장의 mitochondria 및 microtome 획분의 hydroxy radical(.OH)의 생성에 미치는 영향에서 급여식에 사포닌을 첨가한 급여군에서 .OH기의 생성억제 효과를 나타내었다. 간장의 microsome 획 분에서도 0.5 및 1.0% 투여군에서 $H_2O$$_2$생성억제 효과가 인정되었다. 또한 간장의 cytosol획분에서 $O_2$.$^{-}$의 생성은 대조군에 비하여 현저한 억제 효과를 나타내었다. 대두 배아 사포닌의 세포독성을 P338 (mouse Iyoupoid neoplasm)과 L1210 (mouse leukemia) 세포주를 사용하여 실시한 결과 200 $\mu$g/mL 농도에서 높은 성장억제효과를 나타내었다. 그리고 처리 농도에 따른 대두배아 추출물이 P338과 L1210에 대한 성장 효과 실험에서도 200 $\mu$g/mL에서 은 성장억제 효과가 나타났으며 농도가 높을 수록 완전히 억제되는 것을 볼 수 있었다.