Proceedings of the Korea Society of Poultry Science Conference (한국가금학회:학술대회논문집)

The Korean Society of Poultry Science (한국가금학회)
권호 표지

Lectin-binding properties of chicken primordial germ cells during embryonic development

  • Kim, Duk-Kyung (School of Agricultural Biotech) ;
  • Seo, Sam-Youl (School of Agricultural Biotechnology Seoul National University) ;
  • Lee, Eun-Young (School of Agricultural Biotechnology Seoul National University) ;
  • Lee, Seul-Ki (School of Agricultural Biotechnology Seoul National University) ;
  • Han, Jae-Yong (School of Agricultural Biotechnology Seoul National University)
  • Published : 2001.11.01
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Abstract

Lectins have great potential as to determine the alternation of the distribution of cell surface carbohydrates during cellular development and differentiation. Here, we investigated the presence and distribution of cell surface carbohydrates on chicken primordial germ cells (PGCs) during the migration and gonadal stages using a variety of lectins. A total of six FITC-labelled lectins from several specificity classes were used: ConA (glucose/mannose), WGA (N-acetylglucosamine), STA (N-acetylglucosamine), DBA (N-acetylgalactosamine/galactose), UEA-I (fucose) and PHA-E (oilgosaccharide). As a results, PGC-specific binding was observed in STA. PGCs of migration stage (2.5- and 5.5-day embyos) were STA-positive whereas PGCs of 10-day embryonic gonad were not. The results suggest that N-acetylglucosamine residuse are present specifically in migrating chicken PGCs and changes during development.

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