• 식물조직배양학회지
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• 제28권3호
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• pp.159-164
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• 2001

꾸지뽕나무 (Cudrania tricuspidata)에 미량으로 함유되어 있는 신규 생리활성물질인 Gericudranin를 기내 배양세포에서 효율적으로 생산하는 시스템을 확립하기 위하여 캘러스 유도 및 여러 가지 배양조건 규명에 관한 실험을 수행하였다. 캘러스는 1.0 mg/L NAA, 0.1 mg/L kinetin, 3% sucrose가 함유된 B5 배지에 배양된 배축에서 효율적으로 유도되었다. Gericudranin 생산과 세포생장에 관여하는 여러 가지 요인을 조사한 결과, 세포생장을 위해서는 1.0mg/L NAA, 0.1 mg/L zeatin과 5% sucrose가 함유된 WPM배지가 좋았고, Gericudranin 생산을 위해서는 무기염 농도가 1/8로 희석된 MS배지, 1.0 mg/l NAA, 0.1 mg/l zeatin과 5% sucrose 처리구가가장 좋았다 Gericudranin A의 함량 (2200 $\mu\textrm{g}$/g dry wt)은 3% sucrose, 1.0 mg/L IBA가 함유된 1/2MS배지에서 모식물체의 수피의 함량보다 약 10배 높았으며, Gericudranin B의 함량 (2350 $\mu\textrm{g}$/g dry wt)은 sucrose 5%를 함유한 1/2 MS 배지에서 수피의 함량보다 약 3배 높았다.

• 약학회지
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• 제43권5호
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• pp.559-565
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• 1999

The two gericudranin E derivatives, GER-I & II, were synthesized and evaluated their antitumour activities for the elucidation of structure-activity relationship. 2,4,6-Trihydroxyacetophenone was converted to target molecules GER-I and GER-B in 5 steps via sequential protection, aldol condensation, Michael type-cyclization, regioselective C-benzylation. The cellular growth inhibition of compounds GER-I and GER-II were investigated against P388, L1210, K562, HCT-15, SK-HepG-1, MCF-7 as cancer cell lines and mouse splenocytes as a normal cell by MTT assay.

• Journal of Microbiology and Biotechnology
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• 제5권4호
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• pp.234-237
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• 1995

Production of new flavanol derivatives with cytotoxic activity, gericudranin A and B, was studied by using hairy root cultures of Cudrania tricuspidata. Schenk and Hildebrandt (SH) medium was chosen for root growth and gericudranin production. After 35 days culture in a half-strength liquid SH medium containing $30g^{glucose}$/l, hairy root growth reached $138g^{FW}$/I and gericudranin A and B were produced at concentrations of 27mg/l and 21 mg/l, respectively. It was also observed that the contents of gericudranin A and B in hairy root were eight and six times higher than those of cudraniae radix, respectively.

• 약학회지
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• 제41권1호
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• pp.14-17
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• 1997

(${\pm}$)-3-Deoxygericudranin A was designed and synthesized for the development of novel antitumour agent and for the elucidation of the effect of 3-hydroxyl group in gericudranin A on antitumour activity. 2,4.6-Trihydroxyacetophenone was converted to 3-deoxygericudranin A in 5 steps via sequential protection, aldol condensation, Michael tvpe-cyclization, regioselective, C-benzylation and deprotection.

• Natural Product Sciences
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• 제16권2호
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• pp.75-79
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• 2010

Two new benzylated flavonoids, 5,7,4'-trihydroxy-6-p-hydroxybenzylflavanone (1) and 5,7,4'-trihydroxy-6,8-di-p-hydroxybenzylflavanone (2) together with six known flavonoids, kaempferol (3), artocarpesin (4), cycloartocarpesin (5), cudraflavone D (6), gericudranin E (7), and leachianone G (8) have been isolated from the root bark of Cudrania tricuspidata. The structures of 1 and 2 were characterized based on spectroscopic data including 1D- and 2D-NMR. All the isolates were evaluated for their inhibitory effects of monoamine oxidase (MAO). Among them, kaempferol (3), artocarpesin (4), and cudraflavone D (6) showed moderate inhibitory effects with $IC_{50}$ values of 82.3, 30.8, and $71.8\;{\mu}M$, respectively.