• Interdisciplinary Bio Central
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• v.2 no.4
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• pp.12.1-12.7
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• 2010

The National Bio Resource Project-Rat (NBRP-Rat) comprises the largest bank of laboratory rat (Rattus norvegicus) strains in the world. Its main focus is to develop infrastructure that will facilitate the systematic collection, preservation, and provision of rat strains. To breed effectively more than 180 rat strains in living stock, we establish the genetic control system in which a systematic set of genetic diagnoses and genetic monitoring are included. Genetic monitoring is performed by using 20 polymorphic markers. Monitoring is carried out when a living animal stock is re-established by using cryopreserved embryos or sperm or when a rat strain is first introduced to the NBRP-Rat by a depositor. Additional monitoring is then carried out on each strain every two years. Genetic diagnosis is performed largely by employing the Amp-FTA method. Protocols which detail how to perform a genetic diagnosis of 11 transgenes and 24 mutations have been made. Among the mutations, nine can be detected by simple gel electrophoresis of the PCR products, 11 by restriction enzyme treatment of the PCR products, and four by direct PCR product sequencing. Using this genetic control system, the NBRP-Rat can guarantee the genetic quality of its rat strains.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2005.11a
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• pp.323-326
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• 2005

This paper introduces design method for air pump system using bond graph and genetic programming to maximize outflow subject to a constraint specifying maximum power consumption. The air pump system is a mixed domain system which includes electromagnetic, mechanical and pneumaticelements. Therefore an appropriate approach for a better system for synthesis is required. Bond graphs are domain independent, allow free composition, and are efficient for classification and analysis of models, Genetic programming is well recognized as a powerful tool for open-ended search. The combination of these two powerful methods for evolution of multi-domain system, BG/GP, was tested for redesign of air pump system.

• Journal of the Korean Society for Railway
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• v.8 no.5
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• pp.411-418
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• 2005

This paper expounds the development of a user-friendly expert system for the optimal stacking sequence design of composite laminates subjected to the various rules constraints. The expert system was realized in the graphic-based design environment. Therefore, users can access and use the system easily. The optimal stacking sequence is obtained by means of integration of a genetic algorithm, finite element analysis. These systems were integrated with the rules of design heuristics under an expert system shell. The optimal stacking sequence combination for the application of interest is drawn from the discrete ply angles and design rules stored in the knowledge base of the expert system. For the integration and management of softwares, a graphic-based design environment that provides multi-tasking and graphic user interface capability is built.

• BMB Reports
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• v.33 no.6
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• pp.483-492
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• 2000

The Herpes simplex virus type 1 (HSV-1) strain F glycoprotein H (gH) gene in the pHLB-4 plasmid was recombinated into a baculovirus expression vector (lacZ-HcNPV) to construct a recombinant virus GH-HcNPV expressing gH. The sequences of gH and its expression were analyzed. The gH gene was located in the 6.41 kb BglII fragment. The open reading frame (ORF) of the gH gene was 2,517 bp and codes 838 amino acid residues. Insect cells infected with this recombinant virus synthesized a high level of the matured and gX-gH fusion protein with approximately 112 kDa. The fusion gH protein was localized on the membrane of the insect cells as seen by using immunofluorescence assay and accumulated in the cultured media by the SDS-PAGE and immunoprecipitation assays. The amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. Antibodies raised in mice to this recombinant protein recognized viral gH and neutralized the infectivity of HSV-1 in vitro. These results demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV; accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.4
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• pp.91-97
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• 2011

In this paper we present an approach to the structure identification based on genetic algorithm and to the parameter identification by hybrid learning method in neuro-fuzzy-genetic hybrid system in order to predicate the Mackey-Glass Chaotic time series. In this scheme the basic idea consists of two steps. One is the construction of a fuzzy rule base for the partitioned input space via genetic algorithm, the other is the corresponding parameters of the fuzzy control rules adapted by the backpropagation algorithm. In an attempt to test the performance the proposed system, three patterns, x(t-3), x(t-6) and x(t-9), was prepared according to time interval. It was through lots of simulation proved that the initial small error of learning owed to the good structural identification via genetic algorithm. The performance was showed in Table 2.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1080-1085
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• 2005

A hybrid control scheme is proposed for the stabilization of backward movement along simple paths for a vehicle composed of a truck and six trailers. The hybrid comprises the combination of a linear quadratic regulator (LQR) and a neurocontroller (NC) that is trained by a genetic algorithm (GA). Acting singly, either the NC or the LQR are unable to perform satisfactorily over the entire range of the operation required, but the proposed hybrid is shown to be capable of providing good overall system performance. The evaluation function of the NC in the hybrid design has been modified from the conventional type to incorporate both the squared errors and the running steps errors. The reverse movement of the trailer-truck system can be modeled as an unstable nonlinear system, with the control problem focusing on the steering angle. Achieving good backward movement is difficult because of the restraints of physical angular limitations. Due to these constraints the system is impossible to globally stabilize with standard smooth control techniques, since some initial states necessarily lead to jack-knife locks. This paper demonstrates that a hybrid of neural networks and LQR can be used effectively for the control of nonlinear dynamical systems. Results from simulated trials are reported.

• Journal of the Korea Institute of Military Science and Technology
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• v.18 no.6
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• pp.797-807
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• 2015

The low-altitude PAC-2 Patriot missile system is the backbone of ROK air defense for intercepting enemy aircraft. Currently there is no missile interceptor which can defend against the relatively high velocity ballistic missile from North Korea which may carry nuclear, biological or chemical warheads. For ballistic missile defense, Korea's air defense systems are being evaluated. In attempting to intercept ballistic missiles at high altitude the most effective means is through a multi-layered missile defense system. The missile defense problem has been studied considering a single interception system or any additional capability. In this study, we seek to establish a mathematical model that's available for multi-layered missile defense and minimize total interception fail probability and proposes a solution based on genetic algorithms. We perform computational tests to evaluate the relative speed and solution of our GA algorithm in comparison with the commercial optimization tool GAMS.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.8
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• pp.1004-1009
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• 2014

The redundancy allocation problem has usually considered only the component redundancy at the lowest-level for the enhancement of system reliability. A system can be functionally decomposed into system, module, and component levels. Modular redundancy can be more effective than component redundancy at the lowest-level because in modular systems, duplicating a module composed of several components can be easier, and requires less time and skill. We consider a multi-level redundancy allocation problem in which all cases of redundancy for system, module, and component levels are considered. A tabu search of memory-based mechanisms that balances intensification with diversification via the short-term and long-term memory is proposed for its solution. To the best of our knowledge, this is the first attempt to use a tabu search for this problem. Our tabu search algorithm is compared with the previous genetic algorithm for the problem on the new composed test problems as well as the benchmark problems from the literature. Computational results show that the proposed method outstandingly outperforms the genetic algorithm for almost all test problems.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2006.11a
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• pp.298-302
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• 2006

As the age of information technology is coming, companies stress the need of knowledge management. Companies construct ERP system including knowledge management. But, it is not easy to formalize knowledge in organization. we focused on data mining system by using genetic programming. But, we don't have enough data to perform the learning process of genetic programming. We have to reduce input parameter(s) or increase number of learning or training data. In order to do this, the enhanced data mining system by using GP combined with SOM(Self organizing map) is adopted in this paper. We can reduce the number of learning data by adopting SOM.

• BMB Reports
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• v.43 no.3
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• pp.219-224
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• 2010

Lipid peroxidation is known to be an important factor in the pathologies of many diseases associated with oxidative stress. We assessed the lipid peroxidation induced by the reaction of ferritin with $H_2O_2$. When linoleic acid micelles or phosphatidyl choline liposomes were incubated with ferritin and $H_2O_2$, lipid peroxidation increased in the presence of ferritin and $H_2O_2$ in a concentration-dependent manner. The hydroxyl radical scavengers, azide and thiourea, prevented lipid peroxidation induced by the ferritin/$H_2O_2$ system. The iron specific chelator desferoxamine also prevented ferritin/$H_2O_2$ systemmediated lipid peroxidation. These results demonstrate the possible role of iron in ferritin/$H_2O_2$ system-mediated lipid peroxidation. Carnosine is involved in many cellular defense processes, including free radical detoxification. In this study, carnosine, homocarnosine, and anserine were shown to significantly prevent ferritin/$H_2O_2$ system-mediated lipid peroxidation and also inhibited the free radical-generation activity of ferritin. These results indicated that carnosine and related compounds may prevent ferritin/$H_2O_2$ system-mediated lipid peroxidation via free radical scavenging.