• Molecules and Cells
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• 제23권3호
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• pp.323-330
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• 2007

The mature wheat embryo is arguably one of the best explants for genetic transformation because of its unlimited availability and lack of growth season restriction. However, an efficient regeneration system using mature wheat embryos (Triticum aestivum L.) is still not available. To identify genes related to the tissue culture response (TCR) of wheat, QTLs for callus induction from mature embryos and callus regeneration were mapped using an RIL population derived from the cross of 'Wangshuibai' with 'Nanda2419', which has a good TCR. By whole genome scanning we identified five, four and four chromosome regions conditioning, respectively, percent embryos forming a callus (PEFC), percent calli regenerating plantlets (PCRP), and number of plantlets per regenerating callus (NPRC). The major QTLs QPefc.nau-2A and QPcrp.nau-2A were mapped to the long arm of chromosome 2A, explaining up to 22.8% and 17.6% of the respective phenotypic variance. Moreover, two major QTLs for NPRC were detected on chromosomes 2D and 5D; these together explained 51.6% of the phenotypic variance. We found that chromosomes 2A, 2D, 5A, 5B and 5D were associated via different intervals with at least two of the three TCR indexes used. Based on this study and other reports, the TCRs of different explant types of wheat may be under the control of shared or tightly linked genes, while different genes or gene combinations may govern the stages from callus induction to plantlet regeneration. The importance of group 2 and 5 chromosomes in controlling the TCRs of Triticeae crops and the likely conservation of the corresponding genes in cereals are discussed.

• 대한소아신경학회지
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• 제26권4호
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• pp.284-287
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• 2018

결절성경화증은 두 개의 유전자 TSC1 (Encoding hamartin, 9q34)과 TSC2 (Encoding tuberin, 16p13) 중 하나의 돌연변이로 발생하는 상염색체 우성 질환이다. 위 유전자에 문제가 생기면 종양 억제 기능을 수행할 수 없어 과오종이 생기며 뇌, 신장, 심장, 눈, 폐, 기타 다른 기관들에 영향을 미치는 복합성 질환이다. 크론병은 명확하게 밝혀 지지 않은 만성 면역 매개성 질환이다. 이는 유전적 소견이 있는 소화관에 정상적으로 존재하는 세균에 대한 우리 몸의 과도한 면역 반응으로 인한 것으로 생각된다. 위 두 개의 질환이 동시에 발병하는 경우는 없었으나, 본 저자들은 결절성경화증 환자가 크론병을 앓고 있는 증례를 경험하였기에 보고하는 바이다. 12세 남자 환아가 복통을 주소로 내원하였다. 내원 시 결절성경화증에서 보이는 피부 병변이 보였다. 안저 검사 결과 오른쪽 망막에 과오종이 발견되었다. 뇌자기공명영상에서 뇌실막밑거대세포 별아교세포종이 나타났고 결절성경화증으로 진단할 수 있었다. 혈액검사 결과 적혈구 침강 속도와 C-반응성 단백질이 증가한 것으로 나타났다. 복부 초음파 검사에서 모든 근위 결장 및 상행 결장, 회맹판, 말단 회장의 혈류 증가로 벽이 두꺼워져 있었다. 대장 내시경 검사 결과 회장, 회맹판 및 맹장의 불연속 궤양과 염증 소견이 보여 크론병으로 진단되었다. 환아는 뇌실막밑거대세포 별아교세포종의 치료를 위해 everolimus를 경구 투여 하였으나 크론병이 악화되어 사용과 중단을 반복하였다. 결절성경화증에서 크론병의 병발이 가능하고 뇌실막밑거대세포 별아교세포종의 치료에 영향을 줄 수 있으므로 지속적인 복통을 호소하는 환자에서 크론병도 고려해 볼 수 있겠다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2017년도 학술발표회
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• pp.264-264
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• 2017

국외의 상수도 원격검침 시스템 내 데이터 전송방식은 도시 규모, 계량기의 밀도, 전력공급 여부 및 통신망의 설치 여부 등을 종합적으로 고려하여 결정되었다. 대부분의 스마트워터미터 제조업체들은 계량기의 부호기가 공급하는 판독 내용(데이터)을 전송할 검침단말기와 근거리 통신망(neighborhood area network)을 연계하여 개발 및 판매하였으며, 자체 소유 통신 프로토콜을 사용하여 라디오 주파수(RF) 통신 기술을 사용하고 있다. 광역통신망(wide area network)의 경우, 노드(말단의 계량기 및 센서)들과 이에 연결된 통신망 들을 포함한 네트웍의 배열이나 구성이 스타(star), 메쉬(mesh), 버스(bus), 나무(tree) 등의 형태로 통신망이 구성되어 있으나, 스타와 메쉬형 통신망 구성형태가 가장 널리 활용되는 것으로 조사되었다. 시스템 통합운영관리 업체들인 IBM, Oracle, Itron 등은 용수 인프라 관리 또는 통합네트워크 솔루션 등의 통합 물관리 시스템(integrated water management system)을 개발하여 현장적용을 하고 있으며, 원격검침 시스템을 통해 고객들의 현재 소비량과 과거 누적 소비량, 누수 감지 서비스 및 실시간 요금 고지 등을 실시간으로 웹 포털과 앱을 통해 제공하고 있다. 또한, 일부 제조업체들은 도시 용수공급/소비 관리자가 주민의 용수사용량을 모니터링하여 일평균 용수사용량 및 사용 경향을 파악하고, 누수를 검지하여 복구 및 용수 사용 지속가능성 지수를 제시하고, 실시간으로 주민의 용수사용량 관련 데이터를 모니터링하여 용수공급의 최적화를 위한 의사결정지원 서비스를 용수공급자에게 제공하고 있다. 최근에는 인공지능을 활용해 가정용수의 용도별(세탁용수, 화장실용수, 샤워용수, 식기세척용수 등) 사용량 곡선을 패터닝하여 profiling 기법을 도입해, 스마트워터미터에서 용수사용량이 통합되어 검지될 시 용수사용량의 세부 용도별 re-profiling 기법을 도입하여 가정용수내 과소비되는 지점을 도출 후 절감을 유도하는 기술이 개발 중이다. 또한, 미래 용수 사용량 예측을 위해 다양한 시계열 자료를 분석하는 선형 종속 모형(자기회귀모형, 자기회귀이동평균모형, 자기회귀적분이동평균모형 등)과 비선형 종속 모형(Fuzzy Logic, Neural Network, Genetic Algorithm 등)을 활용한 예측기능이 구축되어 상호 비교하여 최적의 용수사용량 예측 도구를 제공되고 있다.

• 한국환경보건학회지
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• 제47권5호
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• pp.479-485
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• 2021

Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.

• Animal Bioscience
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• 제34권6호
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• pp.975-984
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• 2021

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

• 대한상부위장관⦁헬리코박터학회지
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• 제18권3호
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• pp.186-197
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• 2018

Background/Aims: To investigate the predictive factors for improvement of atrophic gastritis (AG) and intestinal metaplasia (IM). Materials and Methods: A total of 778 subjects were prospectively enrolled and followed up for 10 years. Histological analysis of AG and IM was performed by using the updated Sydney system. To find the predictive factors for reversibility of AG and IM, 24 factors including genetic polymorphisms and bacterial and environmental factors were analyzed. Results: In all subjects, the predictive factor by multivariate analysis for improvement of both antral and corpus AG was successful eradication. The predictive factors for improvement of antral IM were age and successful eradication. The predictive factor for improvement of corpus IM was successful eradication. In patients with Helicobacter pylori infection, age and cagA were predictive factors for improvement of AG and IM. In patients with H. pylori eradication, monthly income and cagA were predictive factors for improvement of AG and IM. Conclusions: H. pylori eradication is an important predictive factor of regression of AG and IM and would be beneficial for the prevention of intestinal-type gastric cancer. Young age, high income, and cagA are additional predictive factors for improving AG and IM status. Thus, various factors affect the improvement of AG and IM.

• 지능정보연구
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• 제24권4호
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• pp.155-170
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• 2018

기업의 금융 부도를 예측하는 것은 전통적으로 비즈니스 분석에서 가장 중요한 예측문제 중 하나이다. 선행연구에서 예측모델은 통계 및 기계학습 기반의 기법을 적용하거나 결합하는 방식으로 제안되었다. 본 논문에서는 잘 알려진 최적화기법 중 하나인 시뮬레이티드 어니일링에 기반한 새로운 지능형 예측모델을 제안한다. 시뮬레이티드 어니일링은 유전자알고리즘과 유사한 최적화 성능을 가진 것으로 알려져 있다. 그럼에도 불구하고, 시뮬레이티드 어니일링을 사용한 비즈니스 의사결정 문제의 예측과 분류에 관한 연구가 거의 없었기 때문에, 비즈니스 분석에서의 유용성을 확인하는 것은 의미가 있다. 본 연구에서는 시뮬레이티드 어니일링과 기계학습의 결합 모델을 사용하여 부도예측모델의 입력 특징을 선정한다. 최적화 기법과 기계학습기법을 결합하는 대표적인 유형은 특징 선택, 특징 가중치 및 사례 선택이다. 이 연구에서는 선행연구에서 가장 많이 연구된 특징 선택을 위한 결합모델을 제안한다. 제안하는 모델의 우수성을 확인하기 위하여 본 연구에서는 한국 기업의 실제 재무데이터를 이용하여 그 결과를 분석한다. 분석결과는 제안된 모델의 예측 정확도가 단순한 모델의 예측 정확성보다 우수하다는 것을 보여준다. 특히 기존의 의사결정나무, 랜덤포레스트, 인공신경망, SVM 및 로지스틱 회귀분석에 비해 분류성능이 향상되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.320-333
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• 2019

Objective: The Shanghai Holstein cattle breed is susceptible to severe mastitis and other diseases due to the hot weather and long-term humidity in Shanghai, which is the main distribution centre for providing Holstein semen to various farms throughout China. Our objective was to determine the genetic mechanisms influencing economically important traits, especially diseases that have huge impact on the yield and quality of milk as well as reproduction. Methods: In our study, we detected the structural variations of 1,092 Shanghai Holstein cows by using next-generation sequencing. We used the DELLY software to identify deletions and insertions, cn.MOPS to identify copy-number variants (CNVs). Furthermore, we annotated these structural variations using different bioinformatics tools, such as gene ontology, cattle quantitative trait locus (QTL) database and ingenuity pathway analysis (IPA). Results: The average number of high-quality reads was 3,046,279. After filtering, a total of 16,831 deletions, 12,735 insertions and 490 CNVs were identified. The annotation results showed that these mapped genes were significantly enriched for specific biological functions, such as disease and reproduction. In addition, the enrichment results based on the cattle QTL database showed that the number of variants related to milk and reproduction was higher than the number of variants related to other traits. IPA core analysis found that the structural variations were related to reproduction, lipid metabolism, and inflammation. According to the functional analysis, structural variations were important factors affecting the variation of different traits in Shanghai Holstein cattle. Our results provide meaningful information about structural variations, which may be useful in future assessments of the associations between variations and important phenotypes in Shanghai Holstein cattle. Conclusion: Structural variations identified in this study were extremely different from those of previous studies. Many structural variations were found to be associated with mastitis and reproductive system diseases; these results are in accordance with the characteristics of the environment that Shanghai Holstein cattle experience.

• Animal Bioscience
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• 제34권6호
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• pp.993-1005
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• 2021

Objective: Zearalenone (ZEA) has estrogen-like effects. Our previous study has shown that ZEA (0.5 to 1.5 mg/kg) could induce abnormal uterine proliferation through transforming growth factor signaling pathway. To further study the other regulatory networks of uterine hypertrophy caused by ZEA, the potential mechanism of ZEA on porcine endometrial epithelial cells (PECs) was explored by the Illumina Hiseq 2000 sequencing system. Methods: The PECs were treated with ZEA at 0 (ZEA0), 5 (ZEA5), 20 (ZEA20), and 80 (ZEA80) µmol/L for 24 h. The collected cells were subjected to cell cycle, RNA-seq, real-time quantitative polymerase chain reaction, immunofluorescence, and western blot analysis. Results: The proportion of cells in the S and G2 phases decreased (p<0.05), but the proportion of cells in the G1 phase increased (p<0.05) in the ZEA80 treatment. Data analysis revealed that the expression of Wnt pathway-related genes, estrogen-related genes, and mitogen-activated protein kinase pathway-related genes increased (p<0.05), but the expression of genetic stability genes decreased (p<0.05) with increasing ZEA concentrations. The relative mRNA and protein expression of WNT1, β-catenin, glycogen synthase kinase 3β (GSK-3β) were increased (p<0.05) with ZEA increasing, while the relative mRNA and protein expression of cyclin D1 (CCND1) was decreased (p<0.05). Moreover, our immunofluorescence results indicate that β-catenin accumulated around the nucleus from the cell membrane and cytoplasm with increasing ZEA concentrations. Conclusion: In summary, ZEA can activate the Wnt/β-catenin signaling pathway by up-regulating WNT1 and β-catenin expression, to promote the proliferation and development of PECs. At the same time, the up-regulation of GSK-3β and down-regulation of CCND1, as well as the mRNA expression of other pathway related genes indicated that other potential effects of ZEA on the uterine development need further study.

• 정신신체의학
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• 제28권2호
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• pp.99-107
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• 2020

섬유근통은 피로, 수면장애, 기분변화, 인지장애 및 만성 전신통증을 핵심 증상으로 하는 질환이다. 섬유근통은 유전적 취약성, 통증 처리과정 및 스트레스 반응 체계의 변화 등을 포함하는 생물학적 요인과 불안, 우울, 분노, 스트레스 등의 심리적 요인, 그리고 감염, 발열 질환, 외상 등의 환경적 요인이 함께 작용하여 발생하는 것으로 알려져 있으며, 핵심 병인으로 감각 자극이 인식되는 과정에서 증폭되어 통증이 증진되는 중추성 감작의 역할이 강조되고 있다. 가이드라인들은 개별 환자의 필요에 따라 선택된 다원적 접근을 권고하며, 임상의는 충분한 교육을 통해 지적 이해의 틀을 제공하고 자기 관리의 중요성을 강조해야 한다. 섬유근통에서 정신건강 문제의 유병률은 일반인구집단의 7~9배로 유의하게 높으며, 특정 정신병리 및 수면장애와의 관련성도 제기되고 있다. 정신과적 상태는 섬유근통과 양방향성 상호작용을 하며 경과에 영향을 주고, 취약성을 공유하며 서로의 위험 요인으로 작용할 수 있으므로, 동반질환을 면밀히 평가하여 보다 통합적인 관리가 필요할 것이다.