• 제목/요약/키워드: genetic conservation

검색결과 496건 처리시간 0.025초

tufA gene as molecular marker for freshwater Chlorophyceae

  • Vieira, Helena Henriques;Bagatini, Inessa Lacativa;Guinart, Carla Marques;Vieira, Armando Augusto Henriques
    • ALGAE
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    • 제31권2호
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    • pp.155-165
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    • 2016
  • Green microalgae from the class Chlorophyceae represent a major biodiversity component of eukaryotic algae in continental water. Identification and classification of this group through morphology is a hard task, since it may present cryptic species and phenotypic plasticity. Despite the increasing use of molecular methods for identification of microorganisms, no single standard barcode marker is yet established for this important group of green microalgae. Some available studies present results with a limited number of chlorophycean genera or using markers that require many different primers for different groups within the class. Thus, we aimed to find a single marker easily amplified and with wide coverage within Chlorophyceae using only one pair of primers. Here, we tested the universality of primers for different genes (tufA, ITS, rbcL, and UCP4) in 22 strains, comprising 18 different species from different orders of Chlorophyceae. The ITS primers sequenced only 3 strains and the UCP primer failed to amplify any strain. We tested two pairs of primers for rbcL and the best pair provided sequences for 10 strains whereas the second one provided sequences for only 7 strains. The pair of primers for the tufA gene presented good results for Chlorophyceae, successfully sequencing 21 strains and recovering the expected phylogeny relationships within the class. Thus, the tufA marker stands out as a good choice to be used as molecular marker for the class.

Characterization and functional inferences of a genome-wide DNA methylation profile in the loin (longissimus dorsi) muscle of swine

  • Kim, Woonsu;Park, Hyesun;Seo, Kang-Seok;Seo, Seongwon
    • Asian-Australasian Journal of Animal Sciences
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    • 제31권1호
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    • pp.3-12
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    • 2018
  • Objective: DNA methylation plays a major role in regulating the expression of genes related to traits of economic interest (e.g., weight gain) in livestock animals. This study characterized and investigated the functional inferences of genome-wide DNA methylome in the loin (longissimus dorsi) muscle (LDM) of swine. Methods: A total of 8.99 Gb methylated DNA immunoprecipitation sequence data were obtained from LDM samples of eight Duroc pigs (four pairs of littermates). The reference pig genome was annotated with 78.5% of the raw reads. A total of 33,506 putative methylated regions (PMR) were identified from methylated regions that overlapped at least two samples. Results: Of these, only 3.1% were commonly observed in all eight samples. DNA methylation patterns between two littermates were as diverse as between unrelated individuals (p = 0.47), indicating that maternal genetic effects have little influence on the variation in DNA methylation of porcine LDM. The highest density of PMR was observed on chromosome 10. A major proportion (47.7%) of PMR was present in the repeat regions, followed by introns (21.5%). The highest conservation of PMR was found in CpG islands (12.1%). These results show an important role for DNA methylation in species- and tissue-specific regulation of gene expression. PMR were also significantly related to muscular cell development, cell-cell communication, cellular integrity and transport, and nutrient metabolism. Conclusion: This study indicated the biased distribution and functional role of DNA methylation in gene expression of porcine LDM. DNA methylation was related to cell development, cell-cell communication, cellular integrity and transport, and nutrient metabolism (e.g., insulin signaling pathways). Nutritional and environmental management may have a significant impact on the variation in DNA methylation of porcine LDM.

충남 오서산과 봉수산의 식물상 및 관리방안 (Management Methods and Vascular Plants of the Ohseosan and the Bongsusan, Chungnam)

  • 오현경;김동필;오구균;강기래;배중남
    • 한국환경복원기술학회지
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    • 제16권3호
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    • pp.63-81
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    • 2013
  • The vascular plants in the Ohseosan and the Bongsusan, Chungnam were listed 439 taxa (9.0% of all 4,881 taxa of vascular plants); 95 families, 268 genera, 339 species, 5 subspecies, 75 varieties and 20 forms. Furthermore, the Ohseosan were listed 339 taxa and the Bongsusan were listed 306 taxa. So, Hemicryptophytes (H) were 107 taxa (24.4%), Therophytes (Th), Geophytes (G) same as were 82 taxa (18.7%) showed high proportional ratio in life form. Based on the list of rare plants, 4 taxa; Aristolochia contorta, Viola albida, Scutellaria insignis (LC) and Scrophularia koraiensis (DD) and endemic plants, 8 taxa; Aconitum pseudolaeve, Ajuga spectabilis, etc. Based on the list of specific plants, 35 taxa; Wisteria floribunda for. floribunda in class IV, 5 taxa (Scutellaria insignis, Scrophularia koraiensis, etc.) in class III, 5 taxa (Acer triflorum, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Pyrus ussuriensis var. ussuriensis, Campanula punctata, etc.) in class I. Based on the list of naturalized plants, 9 families, 20 genera, 24 taxa (Persicaria orientalis, Carduus crispus, etc.) and ecosystem disturbing plants were Rumex acetocella, Ambrosia artemisiifolia. Naturalization rate was 5.5% of all 439 taxa of vascular plants and urbanization index was 7.5% of all 321 taxa of naturalized plants. In particular, for rare plants, in and ex-situ conservation of genetic resources must surely be done, by preserving present natural habitats, discovering additional natural habitats and securing seeds. Moreover, ecosystem disturbing plants require long-term monitoring and consistent management, since not only do they disturb the ecosystem in competition with Korean native species, but damage humans, too.

경주개(동경이)의 혈통확인을 위한 microsatellite DNA 다형성 분석 (Analysis of Microsatellite DNA Polymorphisms for Pedigree Verification in Kyungju Dog(Dongkyung-i).)

  • 이은우;최석규;조길재
    • 생명과학회지
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    • 제18권6호
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    • pp.902-906
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    • 2008
  • 경주지방에서 사육 중인 경주개(동경이) 51두를 대상으로 8개의 microsatellite marker을 이용하여 DNA형을 분석한 결과 대립 유전자 수는 $4{\sim}12$개(평균 8.5개)로 검출되었으며 Expected heterozygosit와 PIC는 각각 $0.6162{\sim}0.8746$(평균 0.7587)와 $0.5461{\sim}0.8512$(평균 0.7167)으로 나타났고, PEZ3, PEZ6, PEZ12, FHC2054 marker는 PIC가 0.7이상으로 나타났다. 이들 marker는 향후 동경이의 개체식별 및 친자확인에 유용하게 쓰일 수 있다. 공시재료 51두 중 사육가에 의해 혈통이 알려진 5두를 대상으로 microsatellite marker를 이용하여 혈통을 분석한 결과 3두에서 현재 가계도와 일치하지 않았다. 따라서 앞으로 더 많은 연구를 통해서 동경이에 대한 체계적인 혈통 정립이 이루어져야 할 것이다.

Comparative Study on Various Growth Performances of Korean Oge and White Leghorn Chickens

  • Kang, Kyung-Soo;Jang, Hyun-Jun;Lee, Hyung-Chul;Kim, Sung-Kyu;Kim, Jae-Hwan;Choi, Seong-Bok;Rengaraj, Deivendran;Han, Jae-Yong
    • 한국가금학회지
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    • 제39권2호
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    • pp.105-112
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    • 2012
  • Genetic resource conservation of indigenous species is important to conserve terrestrial, aquatic and avian species throughout the world. The native Korean Oge (KO) chickens are important and protected indigenous avian species in Korea. This KO breed is very popular in Korea because of its external appearances of blackish color. The characterization of morphology and growth performances of KO were performed since 1980s, however the information is still not sufficient for breed selection. In this study, we compared various growth performances including body growth, tibia length, shank length, chest width, chest grith and length of tail feather between male and female chickens of KO and widely known White Leghorn (WL) breeds at 1 week, 2 weeks, 5 weeks, 10 weeks, and 24 weeks. We observed differences on various growth performances at different aged groups between KO and WL chickens. This study may help for the selection of chicken breeds based on age, body growth and meat production.

Modest calcium increase in tomatoes expressing a variant of Arabidopsis cation/$H^+$ antiporter

  • Chung, Mi-Young;Han, Jeung-Sul;Giovannoni, James;Liu, Yang;Kim, Chang-Kil;Lim, Ki-Byung;Chung, Jae-Dong
    • Plant Biotechnology Reports
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    • 제4권1호
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    • pp.15-21
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    • 2010
  • The over-expression of Arabidopsis CAX1 and CAX2 causes transgenic tomato plants to reveal severe $Ca^{2+}$ deficiency-like symptoms such as tip-burn and/or blossom end rot, despite there being sufficient $Ca^{2+}$ in each plant part. To correct the symptoms and to moderately enhance the calcium level, a worldwide vegetable tomato was genetically engineered using a modified Arabidopsis cation/$H^+$ antiporter sCAX2A, a mutant form of Arabidopsis CAX2. Compared with the wild-type, the sCAX2A-expressing tomato plants demonstrated elevated $Ca^{2+}$ levels in the fruits with almost no changes in the levels of $Mn^{2+}$, $Cu^{2+}$, and $Fe^{2+}$. Moreover, expression of sCAX2A in tomato plants did not show any significant alterations in their morphological phenotypes. Unlike 35S::sCAX1 construct, sCAX2A antiporter gene driven by 35S promoter can be a valuable tool for enriching $Ca^{2+}$ contents in the tomato fruit without additional accumulation of the undesirable cations.

국내수목원 조성 및 운영경향에 관한 연구 (A Study on the Composition and Operating Trend of Arboreta in Korea)

  • 김종익;강철기;신현탁;성정원
    • 한국환경복원기술학회지
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    • 제14권5호
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    • pp.37-51
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    • 2011
  • Research and conservation of plant species for the nation arboretum, collection and securing of the plant species for both public and private arboreta and education for the arboreta which is affiliated to the university are overall management goal of the arboreta in Korea. Most of the arboreta in Korea run the programs and events for guide and interpretation of forest, but the programs and events are not well specified by the arboreta. Regarding the reasonable level of entrance fee should be discussed further as the lack of information and understanding. But the specified entrance fee between public and private arboreta should be considered. An eighty three percent of arboreta in Korea has been properly managed the species information. The reasonable management should be considered in case of unusual management of collected plant genetic resources. Although the overall impending difficulties for the management of arboreta in Korea is the lack of trained or qualified staffs, securing tasks independently for the public arboreta and relatively low salary for the private arboreta are main difficulties. Most of the difficulties are derived from the lack of its mission of the arboreta. The management of arboreta by the mission statement is one of the core targets including the characters such as climate or region should be considered. The securing qualified or trained staffs should be considered in the development of arboreta in Korea including the modification of relevant regulations or law.

함백산 산림유전자원보호구역의 식물상 및 현존식생 (Flora and Actual Vegetation Classification of Protected Area for Forest Genetic Resource Conservation in Mt. Hambaek)

  • 이정현;김동갑;신재권;정세훈
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.49-49
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    • 2018
  • 본 연구는 함백산 산림유전자원보호구역의 생물다양성 증진과 체계적이고 객관적인 보전 및 관리를 위한 기초데이타 수립을 위하여 식물상과 정밀식생조사를 수행하였다. 함백산 산림유존자원보호구역에 분포하는 식물상은 83과 222속 321종 2아종 36변종 등 359분류군이 확인되었으며, 희귀 및 멸종위기식물은 산마늘, 등칡 등 총 10과 14분류군이 확인되었다. 식물구계학적 3등급 종은 15과 20분류군, 4등 급종은 8과 11분류군이 확인되었다. 산림청 특별산림보호 대상종은 3과 3분류군, 한국특산식물은 7과 10분류군이 확인되었으며 귀화식물은 9과 16분류군이 출현하였다. 함백산 산림유전자원보호구역 26개 조사구역에 대하여 정밀식생조사를 수행한 결과. 식생유형은 산지관목림, 일본잎갈나무식재림, 가래나무군락, 거제수나무군락, 거제수나무-신갈나무군락, 거제수나무-층층나무군락, 거제수나무-피나무군락, 소나무군락, 신갈나무군락, 피나무군락, 피나무-거제수나무군락 등 총 11개의 유형으로 확인되었다. 또한 식생조사와 드론을 활용하여 현존식생도를 작성하였으며, 그 결과 함백산 유전자원보호구역은 총 470.9ha로, 그 중 신갈나무군락이 총 390.8ha로 가장 넓게 분포하고 있으며, 거제수나무-신갈나무군락이 34.3ha, 산지관목림 18.5ha 순으로 대부분 10ha 이하로 나타났다.

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청옥산 산림유전자원보호구역의 관속식물상 (The Flora of Protected Area for Forest Genetic Resource Conservation in the Mt. Cheongok)

  • 변준기;신재권;김주영;최승호;김동갑
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.46-46
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    • 2018
  • 본 연구는 봉화군 청옥산 산림유전자원보호구역에 분포하는 관속식물을 파악하고자 수행하였다. 청옥산 보호구역은 산림청에서 '희귀식물자생지' 유형으로 지정하여 관리하고 있으며, 면적은 약 2,051ha에 달하며 주로 신갈나무군락과 소나무군락이 우점하고있다. 현지조사는 2017년 3월부터 10월까지 계절별로 수행하였다. 조사결과, 보호구역내 관속식물은 86과 258속 390종 4아종 54변종 6품종으로 총 454분류군의 분포가 확인되었다. 이 중 한국특산식물은 가야물봉선, 처녀치마, 할미밀망, 키버들 등 11분류군이며, 산림청 지정 희귀식물은 취약종(VU)에 주목, 백작약, 꼬리진달래 3분류군, 약관심종(LC)에 등칡, 도깨비부채, 정향나무, 말나리, 나도개감채 등 14분류군이다. 식물구계학적 특정식물로는 IV등급인 회리바람꽃, 가지괭이눈, 산겨릅나무, 귀박쥐나물 등 10분류군, III등급인 애기감둥사초, 중나리, 토현삼, 선갈퀴, 금강제비꽃, 노랑갈퀴, 분비나무 등 23분류군, II등급 32분류군, I등급 30분류군이 확인되었다. 외래식물은 오리새, 털별꽃아재비, 왕포아풀, 주걱개망초, 지느러미엉겅퀴, 족제비싸리 등 18분류군이 임도 주변에서 확인되었다. 조사된 관속식물을 용도별로 구분하면 식용식물 181분류군, 섬유용식물 6분류군, 약용식물 143분류군, 관상용식물 52분류군, 사료용식물 172분류군, 염료용식물 9분류군, 목재용식물 22분류군으로 분석되었다.

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청옥산 산림유전자원보호구역의 산림군락분류 및 임분특성 (Forest Community Classification and Stand Characteristics of Protected Area for Forest Genetic Resource Conservation in Mt. Cheongok)

  • 신재권;변준기;김주영;최승호;김동갑
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.47-47
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    • 2018
  • 본 연구는 청옥산 산림유전자원보호구역을 대상으로 총 49개의 고정방형구를 설치하여 식물사회학적 방법으로 산림군락분류와 임분특성을 분석하였다. 그 결과 총 9개의 식생단위로 분류되었으며 군락단위로는 신갈나무군락, 잣나무군락, 일본잎갈나무군락, 독일가문비나무군락으로 분류되었다. 신갈나무군락은 소나무군, 철쭉군, 고로쇠나무군, 미역줄나무군으로 세분되었으며, 고로쇠나무군은 박달나무소군과 고로쇠나무전형소군으로, 미역줄나무군은 애기감둥사초소군과 미역줄나무전형군으로 세분되었다. 9개의 식생단위의 층위별 중요치 분석결과를 보면 교목층에서 신갈나무가 32.2% 가장 높게 나타났으며 잣나무(10.1%), 소나무(10%), 일본잎갈나무(9.1%) 순으로 나타났고, 아교목층에서는 신갈나무 (18.9%), 당단풍나무(11.7%), 물푸레나무(8.9%), 잣나무(7.2%) 등이 높은 값으로 분석되었다. 관목층에서는 철쭉(12.2%)와 당단풍나무(8%)가 높게 분석되었으며 다른 수종들은 10% 미만의 값을 가졌다. 초본층에서는 조릿대(8.5%), 산숙국(6.6%)등이 높게 나타났다. 식생단위별 종다양성지수를 보면 식생단위 8인 일본잎갈나무군락이 1.76로 가장 높았으며, 박달나무소군 1.73, 소나무군 1.72, 철쭉군 1.71 순으로 분석되었다. 우점도는 모든 식생단위에서 0.2 이하로 다수의 종이 우점하는 것으로 나타났다.

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