Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Modest calcium increase in tomatoes expressing a variant of Arabidopsis cation/$H^+$ antiporter

  • Chung, Mi-Young (Boyce Thompson Institute for Plant Research, Cornell University) ;
  • Han, Jeung-Sul (Department of Ecological Environment Conservation, Kyungpook National University) ;
  • Giovannoni, James (Boyce Thompson Institute for Plant Research, Cornell University) ;
  • Liu, Yang (Boyce Thompson Institute for Plant Research, Cornell University) ;
  • Kim, Chang-Kil (Department of Environmental Horticulture, Kyungpook National University) ;
  • Lim, Ki-Byung (School of Plant Biosciences, Kyungpook National University) ;
  • Chung, Jae-Dong (School of Plant Biosciences, Kyungpook National University)
  • Received : 2009.07.27
  • Accepted : 2009.09.21
  • Published : 2010.03.30
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Abstract

The over-expression of Arabidopsis CAX1 and CAX2 causes transgenic tomato plants to reveal severe $Ca^{2+}$ deficiency-like symptoms such as tip-burn and/or blossom end rot, despite there being sufficient $Ca^{2+}$ in each plant part. To correct the symptoms and to moderately enhance the calcium level, a worldwide vegetable tomato was genetically engineered using a modified Arabidopsis cation/$H^+$ antiporter sCAX2A, a mutant form of Arabidopsis CAX2. Compared with the wild-type, the sCAX2A-expressing tomato plants demonstrated elevated $Ca^{2+}$ levels in the fruits with almost no changes in the levels of $Mn^{2+}$, $Cu^{2+}$, and $Fe^{2+}$. Moreover, expression of sCAX2A in tomato plants did not show any significant alterations in their morphological phenotypes. Unlike 35S::sCAX1 construct, sCAX2A antiporter gene driven by 35S promoter can be a valuable tool for enriching $Ca^{2+}$ contents in the tomato fruit without additional accumulation of the undesirable cations.

Keywords

References

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