• Journal of Korean Academy of Nursing
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• v.25 no.3
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• pp.485-495
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• 1995

The purpose of this study was to contribute to family nursing aimed at reducing stress and improving the coping abilities of parents with kindergarten or early primary school aged children. Data were collected through self- reported questionnaires over a period of one month between November 1994 and December 199t in the Kyoung-in area. The subjects consisted of 198 parents (99 mothers and 99 fathers) of children attending 1 elementary school and 2 kindergartens. The levels of general stress and of parental role stress were measured with the General stress scale and the Parental role stress scale, respectively, while the Coping scale was used to measure the level of coping. The data were analyzed by a SAS program using paired't-test and oneway ANOVA. The results were as follows : 1. The level of general stress was significantly higher in mothers than in fathers. Mothers experienced significantly greater level of parental role stress than fathers did. In contrast, fathers revealed significantly greater scores in coping than mothers. 2. General stress experienced by fathers was different according to education, occupation, health status, satisfaction with family life and support from spouse. Occupation, health status, satisfaction with family life, satisfaction with spouse and support from spouse influenced parental role stress experienced by fathers. There was no correlation between level of coping and general characteristics. 3. In mothers, the level of general stress was different according to their health status, family type, and number of children, while parental role stress was related to satisfaction with family life, satis-faction with spouse and family type. There was no correlation between level of coping and general characteristics. The above findings indicate that the mothers did not develop more coping strategies than the fathers, despite their experience of greater stress than the fathers. Hence, nursing intervention for managing stress and improving coping abilities should be provided for mothers. In particular, fathers should actively participate in parenting, and support their spouse.

• Archives of Pharmacal Research
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• v.29 no.11
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• pp.1032-1041
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• 2006

Extracellular adenosine 5'-triphosphate (ATP) affects the function of many tissues and cells. To confirm the biological activity of ATP on human myeloid leukemic cells, F-36P and HL-60, cells were treated with a variety of concentrations of ATP. The stimulation with extracellular ATP induced the arrest of cell proliferation and cell death. from the analysis of Annexin-V staining and caspase activity by flow cytometry. The Annexin-V positive cells in both cell lines were dramatically increased following ATP stimulation. The expression of P2 purinergic receptor genes was confirmed, such as P2X1, P2X4, P2X5, P2X7 and P2Y1, P2Y2, P2Y4, P2Y5, P2Y6, P2Y11 in both leukemic cell lines. Interestingly, ATP induced intracellular calcium flux in HL-60 cells but not in F-36P cells, as determined by Fluo-3 AM staining. Cell cycle analysis revealed that ATP treatment arrested both F-36P and HL-60 cells at G1/G0. Taken together, these data showed that extracellular ATP via P2 receptor genes was involved in the cell proliferation and survival in human myeloid leukemic cells, HL-60 and F-36P cells by the induction of apoptosis and control of cell cycle. Our data suggest that treatment with extracellular nucleotides may be a novel and powerful therapeutic avenue for myeloid leukemic disease.

• Journal of The Korean Association For Science Education
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• v.44 no.2
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• pp.209-217
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• 2024

This study aimed to analyze the learning contents related to biotechnology in life science II and general engineering based on the 2015 revised national curriculum and discuss pedagogical implications for biotechnology education. The main findings were as follows: First, while the learning topics of life science II focused on the ethical aspects related to biotechnology, the learning topics of general engineering focused on the industrial aspects of biotechnology. Second, there were types of overlapping biotechnologies in the life science II textbooks and general engineering textbooks. However, the life science II textbooks provided more scientific and professional descriptions of biotechnology principles than the general engineering textbooks. Also, the life science II textbooks provided more specific overall explanations than the general engineering textbooks. There was a lack of ethical considerations concerning biotechnology in the general engineering textbooks. These findings imply that it is necessary to reorganize biotechnology content based on each school's curriculum and student characteristics.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.390-390
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• 2005

• Journal of the Korean Home Economics Association
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• v.36 no.2
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• pp.47-60
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• 1998

The effects of personal(gender, physical growth) and environmental(communication with parent, intimacy of friendship, school performance, and satisfaction of school-life) factors on adolescent's self-esteem were examined in a samlpe of 525 first and second grades in middle school. The subdomains of the self-esteem were peer-related self, home self, teacher-related self, academic self, physical appearance self, physical competence self, personality self, and general self. T-test, Pearson's correlation, and regression were used as statistical analysis. Results were as follows. First, there was evidence of a gender difference in the level of the subsdomains of self-esteem: teacher-related, physical-appearance, physical-competence, and personality. Second, the factor which was the most powerful predictor of each subdomain of the self-esteem was as follows 1) the most powerful predictor of the peer-related self was the intimacy of friendship, 2) the most powerful predictor of the home self was the communication with parent, 3) the most powerful predictor of the teacher-related self was the satisfaction of school-life, 4) the most powerful predictor of the academic self was the school performance, 5)the most powerful predictor of the physical-appearance self, the physical competence self, and the personality self was the satisfaction of school-life, 6) the most powerful predictor of the general self was the school performance.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.2
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• pp.226-234
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• 2005

A study was conducted to improve satisfaction to school food service and to furnish source materials for nutrient education by researching degree of satisfaction of middle school students to school food service. Following results are from the study that targeted 215 male students and 173 female students in 5 middle schools in Daegu. Satisfaction rates regarding taste of foods, smell, proper temperature, use of seasonal food, cafeteria atmosphere, numbers of side dishes, and kindness of the cook in a cafeteria were higher than dissatisfaction. And dissatisfaction rates were higher in quantity and quality of foods, sanitation, purity of table wares, opening to nutrient education and related informations for students, opinion reflection of students, food serving time and gratification to food serving than the opposite. But the degree of general satisfaction on school food service were higher than the degree of general dissatisfaction. Sanitation was the first thing to be changed in school food service, then quantity of food and taste for the last. And there were notable differences between male students and female students in sanitation satisfaction. The variables that influence on satisfaction to school food service were sanitary administration, numbers of side dishes, quantity of foods, nutrient, smell and taste when ranked. And the main three things that have similar rate in quantity with satisfaction rate are taste, smell and nutrient of school food service. Through these followed results, degree of general satisfactions to school food service were higher than degree of general dissatisfactions. But sanitation, quantity and quality of foods, taste, and food serving size were not satisfactory for students in rate. Therefore it has to be improved to enhance satisfactions to middle school food service.

• Animal cells and systems
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• v.8 no.3
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• pp.205-212
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• 2004

The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site-directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.

• The Korean Journal of Community Living Science
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• v.25 no.4
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• pp.435-448
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• 2014

This study analyzes the differences in school life satisfaction according to middle school students' general characteristics, including their gender, grade, monthly household income, and parents' education level, and examines the effects of intimacy with parents, optimism, and learning motivation on school life satisfaction. Data were collected from 380 middle school students by using a socio-demographic questionnaire. A measurement tool for intimacy with parents, optimism, learning motivation, and school life satisfaction was used, and a frequency analysis, a t-test, a one-way ANOVA, a correlation analysis, and a path analysis were conducted. School life satisfaction showed differences in terms of middle school students grade and, parental education but there were no meaningful differences in terms of their gender and monthly household income. Intimacy with parents and optimism had direct effects on motivation; intimacy with the father and optimism had direct and indirect effects, respectively; and intimacy with the mother had an indirect effect. An increase in the level of intimacy between parents and students and an increase in the positive perception of oneself facilitated learning motivation as well as school life satisfaction.

• Journal of the Korean Society of School Health
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• v.28 no.2
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• pp.111-119
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• 2015

Purpose: The purpose of this study is to examine the influence of laughter therapy programs on middle school students' happiness, self-esteem and school life adaptation. Methods: In total, 60 students (Experimental group= 30. Control group=30) were recruited to answer a self-administered questionnaire. The questionnaire was composed of questions about general characteristics, happiness, self-esteem and school life adaptation. The experimental group participated in a 45-minute laughter therapy program once a week for eight weeks, while the control group did not. Results: The scores of happiness(t=-4.68, p<.001), self-esteem(t=-3.38, p=.001), and school life adaptation(t=-3.76, p<.001) in the experimental group got significantly higher than the control group. Conclusion: Based on this study results, laughter therapy programs should be applied to improve happiness, self-esteem, and school life adaptation of middle school students.

• Research in Community and Public Health Nursing
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• v.31 no.1
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• pp.86-95
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• 2020

Purpose: The aim of this study was to examine the correlation between school-life adjustment and suicidal ideation in adolescents by applying the latent growth curve models to the longitudinal data. Methods: This study analyzed three waves of data from the Korean Children & Youth Panel Survey (2014~2016). A total of 1,534 students were included in the analysis. In the application of the latent growth curve models to the longitudinal survey data, we analyzed the initial status and growth changes for each wave, identified individual differences in the general characteristics, and examined the direct relationship between the two latent constructs. Results: The analysis revealed that variations in the initial status and rate of school-life adjustment were significant with respect to parents' education level, household income and academic satisfaction. Variations in the initial status and growth rate of suicidal ideation were significantly associated with household income and family structure. The relationship between school-life adjustment and suicidal ideation showed a negative correlation in which the starting value of the former increased and that of the latter decreased and vice versa. Conclusion: The results confirmed that school-life adjustment and suicidal ideation varied according to students' general characteristics. In addition, school-life adjustment was negatively correlated with suicidal ideation, thereby highlighting students' ability to adjust as an important factor influencing their suicidal thinking.