• The Korean Journal of Food And Nutrition
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• v.11 no.5
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• pp.506-512
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• 1998

In order to replance systhetic colors by natural colors as food additive, properties of Gardenia yellow color and Gardenia blue color were compared with Food yellow No. 4 and Food blue No. 1. Color differeance between Food yellow No. 4 and Gardenia yellow color was 7.55. Thermal stability of Food yellow No. 4 was above 99%. On the other hand, in case of Gardenia yellow color, showed adove 90% of residual color units in 8$0^{\circ}C$$\times$30min and 10$0^{\circ}C$$\times$30min at pH 7.0 but 75% in 121$^{\circ}C$$\times$15min. Difference of light stability between Food yellow No. 4 and gardenia yellow color was about 18%. Addition of ascorbic acid was increased about 6% in light stability. Color difference between Food blue No. 2 and Gardenia blue color was 107. Thermal stability of Food blue No. 2 was above 99%. But Gardenia blue color showed 92% of residual color units in 8$0^{\circ}C$$\times$30min and 10$0^{\circ}C$$\times$30min at pH 7.0 but 90% in 121$^{\circ}C$$\times$15min. Difference of light stability between Food blue No. 4 and Gardenia blue color was about 8%. Addition of -tocopherol was increased about 4% in light stability of Gardenia blue color.

• Textile Coloration and Finishing
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• v.13 no.6
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• pp.374-380
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• 2001

Cotton and ramie fabrics were dyed with artemesia and gardenia dyeing solutions. The chroma values of the fabrics dyed with gardenia were higher than that of artemesia. The ramie fabrics dyed with gardenia showed higher chroma values than the cotton fabrics. The colors of fabrics were deeper in shade but their chroma values were improved no more by repetition of dyeing process in artemesia dyebath. The two ways of dyeing processes were carried out. In the first wan, each fabric was dyed in 5 different concentration(1∼5%) of gardenia dyebath to get the fabrics dyed in different shades. After then the gardenia dyed fabrics were dyed again in the artemesia dyebaths. In the second way, the 5 fabrics were dyed in different shades by repetition of dyeing process in artemesia dyebath. After then the artemesia dyed fabrics were dyed again with the gardenia dyebaths. When the artemesia dyeing process was added on the fabrics dyed in five shades with gardenia, the color differences between five samples were slight. But when gardenia dyeing process was added on the fabrics dyed in five shades with artemesia, the samples showed different hue of colors between yellow and green of Munsell color circle. Like almost of fabrics dyed with Plants materials, the colorfastness to light and laundering of the dyed samples were poor But the colorfastness to drycleaning was good.

• Textile Coloration and Finishing
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• v.13 no.6
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• pp.16-16
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• 2001

Cotton and ramie fabrics were dyed with artemesia and gardenia dyeing solutions. The chroma values of the fabrics dyed with gardenia were higher than that of artemesia. The ramie fabrics dyed with gardenia showed higher chroma values than the cotton fabrics. The colors of fabrics were deeper in shade but their chroma values were improved no more by repetition of dyeing process in artemesia dyebath. The two ways of dyeing processes were carried out. In the first way, each fabric was dyed in 5 different concentration(1∼5%) of gardenia dyebath to get the fabrics dyed in different shades. After then the gardenia dyed fabrics were dyed again in the artemesia dyebaths. In the second way, the 5 fabrics were dyed in different shades by repetition of dyeing process in artemesia dyebath. After then the artemesia dyed fabrics were dyed again with the gardenia dyebaths. When the artemesia dyeing process was added on the fabrics dyed in five shades with gardenia, the color differences between five samples were slight. But when gardenia dyeing process was added on the fabrics dyed in five shades with artemesia, the samples showed different hue of colors between yellow and green of Munsell color circle. Like almost of fabrics dyed with Plants materials, the colorfastness to light and laundering of the dyed samples were poor. But the colorfastness to drycleaning was good.

• Molecular & Cellular Toxicology
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• v.5 no.3
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• pp.257-264
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• 2009

Gardenia yellow, extracted from gardenia fruit, has been widely used as a coloring agent for foods, and thus, safety of its usage is of prime importance. In the current study, short-term genotoxicity assays were conducted to evaluate the potential genotoxic effects of gardenia yellow. The gardenia yellow used was found to contain 0.057 mg/g of genipin, a known biologically active compound of the gardenia fruit extract. Ames test did not reveal any positive results. No clastogenicity was detected by a chromosomal aberration test, even on evaluation at the highest feasible concentration of gardenia yellow. Gardenia yellow was also shown to be non-genotoxic using an in vitro comet assay and a micronucleus test with L5178Y cells, although a marginal increase in DNA damage and micronuclei frequency was reported in the respective assays. Additionally, in vivo micronucleus test results clearly demonstrated that oral administration of gardenia yellow did not induce micronuclei formation in the bone marrow cells of male ICR mice. Taken together, our results indicate that gardenia yellow is not mutagenic to bacterial cells, and that it does not cause chromosomal damage in mammalian cells, either in vitro or in vivo.

• The Korean Journal of Food And Nutrition
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• v.11 no.6
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• pp.606-611
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• 1998

For waste recyle, we were investigated on Gardenia blue color production using Gardenia by-product by Bacillus subtilits. Optimum conditions for producing blue pigment were found to be 30$^{\circ}C$, initial pH 6.5, glucose as a carbon source 3% and yeast extract as a nitrogen source 0.5%, respectively. Optimum conditions for fermentor culture were agitation speed 400rpm, aeration 2 vvm and inoculum 5%. The optimum perculture time for inoculum was 20 hrs for blue pigment production.

• 대한상한금궤의학회지
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• v.4 no.1
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• pp.29-36
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• 2012

Aim : To investigate the hepatotective effect of Gardenia jsaminodes and Glycine max aqueous extract against D-galactosamine (d-GalN, 300mg/kg body weight) was administered to the male Sprague Dawley (SD) rats. Materials and Methods : The study was carried out on male SD rats (age matched, weight $250{\pm}10g$). Experimental groups divided four: Normal group (Nor) was administered saline, Control (Con) group was administered saline after d-GalN treatment. Experimental group (Exp) was administered Gardenia jsaminodes (200 mg/kg; Ga group), Glycine max (700 mg/kg; Gl group), and Gardenia jsaminodes+Glycine max (200 mg/kg+700 mg/kg, GG group) during 14 days(n=5). Results : d-GalN administration induced hepatotoxicity in rats which was manifested by increased levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase but decreased total cholesterol and triglyceride. Treatment with Gardenia jsaminodes extract significantly protected the liver in d-GalN administered rats. Conclusion : Gardenia jsaminodes aqueous extract and Gardenia jsaminodes+Glycine max extract possesses hepatoprotective potential, thus validating its use in alleviating toxic effects of d-GalN.

• The Plant Pathology Journal
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• v.18 no.4
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• pp.228-230
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• 2002

Anthracnose symptoms caused by Glomerella cingulata were observed on leaves and stems of gardenia in Sunchon, Jeonnam in Korea in 2000. Symptoms on infected plants typically appeared as irregularly circular, dark-brown ring spots and water-soaked brown lesions. Based on cultural and morphological characteristics, the fungus (G-00-03 isolate) from the diseased plants was identified as Colletotrichum gloeosporioides, and its teleomorph stage was Glomerella cingulata. Healthy gardenia artificially inoculated with fungal spores showed anthracnose symptom 7 days after inoculation. This is the first report of gardenia anthracnose caused by Glomerella cingulata in Korea.

• Food Science and Biotechnology
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• v.18 no.4
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• pp.867-871
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• 2009

Response surface methodology (RSM) was used for the optimization of antioxidant capacity in gardenia extracts. The antioxidant capacities of gardenia fruit extracts were investigated by ferric reducing ability (FRA) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (RSA) assays. The optimum extraction parameters for the strongest antioxidant capacity were the ethanol concentration (EtOH) of 48.9%, extraction temperature of $72.9^{\circ}C$, and extraction time of 29.9 min. Analysis of variance (ANOVA) showed that the quadratics of EtOH and extraction temperature had highly significant effect on the antioxidant capacity (p<0.001). The antioxidant capacity was correlated with contents of bioactive components [crocin, geniposide, and total phenolic (TP) compounds] in gardenia extracts and mainly attributed to the content of the TP compounds.

• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.413-415
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• 2009

To develop a new traditional rice wine by using Gardenia jasminoides, various fermentation methods such as without cooking, with cooking and with starter seed methods were studied. The condition of alcohol fermentation was investigated by addition of 1% Gardenia jasminoides into mash. Among the fermented methods, the fermentation with starter seed was the best as the alcohol was 19%. The acceptability of the Gardenia jasminoides rice wine with different methods were compared. The starter seed method which was prepared by adding 1% Gardenia jasminoides into mash showed the best acceptability in the sensory evaluation test and color test.

• Food Science and Biotechnology
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• v.15 no.5
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• pp.735-738
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• 2006

We examined the neuroprotective and neurotrophic effects of genipin fractionated from gardenia (Gardenia jasminoides Ellis) originating from Korea. The neurotrophic effects of the genipin containing fraction was evaluated by microscopically monitoring its potency to induce neurite outgrowth in PC12h cells. The genipin containing fraction from Korean gardenia promoted neurite outgrowth in PC12h cells in this study, similar to previously reported effects by Wako Chemical, Japan. When cells were treated with the genipin containing fraction prior to ${\beta}$-amyloid peptide treatment (active domain of A peptide 25-35 treated), toxicity was significantly diminished (p<0.0l). These results suggest that genipin prepared from Korean gardenia might potentially be used as a precautionary agent in neurodegenerative disease, such as Alzheimer's disease, etc.