• Korean Journal of Veterinary Research
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• v.22 no.2
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• pp.115-119
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• 1982

Total protein levels of 60 bovine bull sera were treasured with refractometer and the strum proteins were fractionated by cellulose acetate membrane electorphoresis and the relative amount of each fraction was measured with automatic scanning densitometer. The sixty bovine bulls consisted of 17 Charolais bulls, 28 Holstein bulls and 15 Korean bulls. Mean total serum protein level of the total bulls was $7.50{\pm}0.50(Mean{\pm}SD)$, with the mean of Charolais $7.04{\pm}0.37g/dl$, the mean of Holstein $7.62{\pm}0.40g/dl$ and the mean of Korean $7.81{\pm}0.43g/dl$. As barbital-calcium buffer was used in fractionating the bovine serum protein with cellulose acetate membrane, electric current of 0.4 mA per centimeter width of the membrane for an hour resulted in more clear separation between ${\alpha}_1$-globulin and ${\alpha}_2$-globulin than electric current of 0.6 or 1.0 mA for an hour. However ${\alpha}_1$-globulin and ${\alpha}_2$-globulin could not be measured separately with automatic scanning densitometer. Mean serum albumin level of total bulls was $3.54{\pm}0.42g/dl$, with the mean of Charolais $3.42{\pm}0.26g/dl$, the mean of Holstein $3.69{\pm}0.38g/dl$ and the mean of Korean $3.39{\pm}0.55g/dl$. Mean serum ${\alpha}$-globulin level of the total bulls was $0.62{\pm}0.14g/dl$, with the mean of Charolais $0.61{\pm}0.07g/dl$, the mean of Holstein $0.60{\pm}0.11g/dl$ and the mean of Korean $0.67{\pm}0.23g/dl$. Mean ${\beta}$-globulin level of the total bulls was $0.85{\pm}0.21g/dl$, with the mean of Charolais $0.74{\pm}0.11g/dl$, the mean of Holstein $0.83{\pm}0.14g/dl$ and the mean of Korean $1.02{\pm}0.30g/dl$. Mean serum ${\gamma}$-globulin level of the total bulls was $2.48{\pm}0.45g/dl$ with the mean of Charolais $2.27{\pm}0.37g/dl$, the mean of Holstein $2.48{\pm}0.44g/dl$ and the mean of Korean $2.72{\pm}0.47g/dl$.

• Journal of Life Science
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• v.8 no.3
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• pp.326-332
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• 1998

Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purifice enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and $60^{\circ}C$, respectively. The enzyme was stable at $50^{\circ}C$ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the $^{\alpha}$, $^{\beta}$, $^{\gamma}$-CD ratio of 42:46:12. Amylopectin was the most suitable substrate with 67% conversion to CD.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.2
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• pp.333-337
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• 1998

This study was carried out to get infomation on the nitric oxide production ability and its formation mechanisms of polysaccharides extracted from Ganoderma lucidum(PSG) by using murine macrophage cell line. The cultured mycelial cells of Ganoderma lucidum were extracted by alkali, and than neutralized by acid. The extract were passed through the column of DEAE cellulose for more purification. The neutral fraction was concentrated and precipitated with 95% ethanol. The precipitate was lyophilized and PSG was obtained. The immunomodulating effects of PSG on macrophage were performed by using murine macrophage cell line ATCC TIB 71 cells with PSG 0.5mg. PSG alone could not induce the production of nitrite, but it had a significant potential effect on nitrite secretion when the cells were primed and triggered with BCG and Interferon(IFN)-${\gamma}$. Also it was prominent by using calcium channel blocker(verapamil) and adenylate cyclase activator(forskolin).

• Radioisotope journal
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• v.21 no.4
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• pp.46-54
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• 2006

Biocompatible and biodegradable hydrogels based on carboxymethyl cellulose(CMC) and polyethyleneglycol(PEG) were prepared far physical barriers for preventing surgical adhesions. These interpolymeric hydrogels were synthesized by a gamma irradiation crosslinking technique. The 1Scmxl.Scm of cecal serosa and adjacent abdominal wail were abraded with bane burr until tbe serosal surface was disrupted and hemorrhagic but not perforated. and the serosa of tbe cecum was sutured to the abdominal wall in 5mm apart from the injured sire. The denuded cecum was covered with either CMC/PEG hydrogels or solution from CMC/PEG hydrogel. Control rat serosa was not covered. Two weeks later. the rats were sacrificed and adhesion was scored on a 0-5 scale. No treatment showed the significantly higher incidence of adhesions than either CMC/BEC hydrogels or solution from CMC/PEG hydrogel. In conclusion, these studies demonstrate that CMC/BEG hydrogels have a function of prevention of intra abdominal adhesion in a rat model.

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.119-125
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• 1999

The concentration of serum proteins, lipoproteins, leukocytes and lymphocytes in bursectomy and thymectomy Ogol chickens were examined from 5 days to 150 days of age. Concentration of serum proteins and lipoproteins were measured by cellulose acetate electrophoresis. The results summarized as follows : 1. The gamma-globulin concentrations in growing Korean native chickens and Ogol chickens were increased 0.41$\pm$0.01~0.85$\pm$0.05 mg/㎗ and 0.50$\pm$0.01~0.98$\pm$0.08 mg/㎗ from 1 day after to 5 days of age and gradually decreased at 10 days of age, and thereafter increased 0.82$\pm$0.06 mg/㎗, 1.09$\pm$0.04 mg/㎗ at 100 days of age. 2. The serum apha-, beta-, gamma-lipoprotein concentrations of growing Ogol chickens were 74.1$\pm$6.8~240.2$\pm$9.7 mg/㎗, 89.7 5.7~225.8$\pm$11.3 mg/㎗ and 87.6$\pm$4.7-220.3$\pm$10 mg/㎗, respectively. The serum -lipoprotein concentrations from 5 days to 150 days of age Ogol chickens were significantly decreased. 3. The leukocyte and lymphocyte counts in bursectomy and thymectomy Ogol chickens slightly increased from 10 to 100 days of age. However the leukocyte and lymphocyte counts in thymectomy chickens were lower than in untreated chickens. 4. The Ig concentrations of 1 day to 50 days(0.30$\pm$0.03~0.58$\pm$0.04 g/㎗) in bursectomy chickens were similar to those found in untreated chickens and thereafer increased from 50 to 150 days of age(0.58$\pm$0.04~1.21$\pm$0.05 g/㎗). The Ig concentrations in thymectomy chickens increased significantly.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.431-435
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• 2016

The aim of this study was to investigate the effect of gamma irradiation on the pretreatment of wood chips with NaOH solution. The degree of saccharification was quantified by measuring reducing sugar and glucose concentrations after enzymatic hydrolysis. After pretreatment with 10 g/L NaOH, the wood chips were irradiated at the doses of 0, 50, 100, and 200 kGy, respectively. Among the irradiated samples, wood chips irradiated at the dose of 200 kGy had the highest reducing sugar concentration of 12.2 g/L. Also, to define the effect of irradiation before pretreatment, the wood chips were first gamma-irradiated and then pretreated with NaOH. When the NaOH treatment was conducted after irradiation at 200 kGy, the reducing sugar content was further increased to 13.4 g/L and glucose content of the wood chip was as high as 7.9 g/L. These results suggest that gamma irradiation may be the promising method for pretreatment of cellulose biomass.

• Restorative Dentistry and Endodontics
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• v.2 no.1
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• pp.20-26
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• 1976

Cellulose acetate electrophoresis was used for differential diagnosis of radicular cysts and granulomas. Sixteen periapical lesions were excised from sixteen teeth with radiographic evidence of periapical pathosis, and two normal periodontal tissues were surgically removed from two wisdom teeth. The tissue samples were separated into two parts. Half was prepared for cellulose acetate electrophoresis, and the electrophoretic patterns were scanned with Helena densitometer. The other half was examined histologically, and the histologic diagnoses were then compared with the electrophoretic patterns and with the x-ray findings. The results were the following: 1. The histopathologic results showed the presence of 13 granulomas (81.8%) and 3 cysts (18.3%). 2. An albumin pattern, alpha-globulin pattern, beta-globulin pattern, fibrinogen pattern and gamma-globulin pattern were found in all eighteen cases. 3. All eighteen cases were divided into normal periodontal tissue group, dental granuloma group, and radicular cyst group by the histopathologic diagnosis. The electrophoretic pattern of dental granuloma group and that of normal periodontal tissue group resembled each other. The two groups showed the highest percentage in beta-globulin fraction, and the beta-globulin of dental granuloma group(47.17%) was higher than that of normal periodontal tissue group (40.54%) by 6.63%. On the contrary, beta-globulin fraction of radicular cyst group (28.00%) was much lower than those of the granuloma group (47.17%) and normal periodontal tissue group (40.54%), and alpha-globulin of radicular cyst group (34.16%) was much higher than those of the granuloma group (20.04%) and normal group (19.58%). 4. Five cases (31.3%) of the sixteen periodontal lesions showed different results between histopathologic diagnoses and radiographic findings.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.209-220
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• 2015

In this study, gut bacterial communities in xylophagous insects were analyzed using the pyrosequencing of 16S rRNA genes for their potential biotechnological applications in lignocelluloses degradation. The result showed that operational taxonomic units (OTUs), species richness and diversity index were higher in the hindgut than in the midgut of all insect samples analyzed. The dominant phyla or classes were Firmicutes (54.0%), Bacteroidetes (14.5%), ${\gamma}-Proteobacteria$ (12.3%) in all xylophagous insects except for Rhinotermitidae. The principal coordinates analysis (PCoA) showed that the bacterial community structure mostly clustered according to phylogeny of hosts rather than their habitats. In our study, the two carboxymethyl cellulose (CMC)-degrading isolates which showed the highest enzyme activity were most closely related to Bacillus toyonensis $BCT-7112^T$ and Lactococcus lactis subsp. hordniae $NCDO\;2181^T$, respectively. Cellulolytic enzyme activity analysis showed that ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$ and ${\beta}-1,4-xylanase$ were higher in the hindgut of Cerambycidae. The results demonstrate that xylophagous insect guts harbor diverse gut bacteria, including valuable cellulolytic bacteria, which could be used for various biotechnological applications.

• Korean Journal of Food Science and Technology
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• v.49 no.3
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• pp.252-257
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• 2017

In this study, the physicochemical properties of irradiated (gamma-ray and electron-beam) soybeans with different processing (dry and powder) and origins (Korea, China, and USA) were investigated and compared. The results of photostimulated luminescence (PSL) screening indicated that all non-irradiated soybeans showed photon counts (PCs) ${\leq}700$, while all irradiated soybeans showed positive values-gamma-ray 5,815-39,591 count/min; electron beam 5,791-60,055 count/min. The results of thermoluminescence (TL) analysis of all irradiated soybeans indicated that the $TL_1$ glow curves exhibited maximum peaks at 150-250. TL ratio of irradiated samples was ${\geq}0.1$; therefore, the clear identification of irradiated samples was guaranteed by analysis of the $TL_1$ curve shape and TL ratios. The results of electron spin resonance (ESR) signal of 3 irradiated and dried soybeans showed two side peaks mutually spaced at 6.0 mT (cellulose radical). Non-specific signal was detected for all irradiated soybean powders; hence, ESR analysis could not be performed.

• Journal of Radiation Industry
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• v.6 no.4
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• pp.305-310
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• 2012

This study examined radiation detection properties of imported perilla seeds, almonds, and soybeans. Food samples were irradiated at doses of 0.5, 1, 2, and 4 kGy and analyzed by photo-stimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance (ESR). The results of PSL showed that photon counts of irradiated perilla seeds and soybeans were $32,959counts{\cdot}60sec^{-1}$ and $7,234counts{\cdot}60sec^{-1}$, respectively, at an irradiation dose of 0.5 kGy, and the photon count of irradiated almonds was $5,581counts{\cdot}60sec^{-1}$ at an irradiation dose of 1 kGy. The results of TL showed that this technique is useful for detecting irradiated samples; the TL ratios ($TL_1/TL_2$) measured for the food samples at an irradiation dose of 0.5 kGy were 0.2301 for perilla seeds, 0.4595 for almonds, and 0.4827 for soy beans. Lastly, results of ESR spectroscopy for only soybeans revealed specific signals derived from free radicals captured in the cellulose. In conclusion, PSL, TL, and ESR methods can be used for the detection of gamma-irradiated soybean samples, whereas gamma-irradiated perilla seeds and almonds can be confirmed by a serial detection with a TL method after PSL.