• Journal of Plant Biotechnology
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• 제39권1호
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• pp.13-22
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• 2012

Plant regeneration has been optimized increasingly by organogenesis and somatic embryogenesis using a range of explants with tissue culture improvements focusing on factors, such as the age of the explant, genotype, media supplements and $Agrobacterium$ co-cultivation. The production of haploids and doubled haploids using microspores has accelerated the production of homozygous lines in Brassicaceae crop plants. Somatic cell fusion has facilitated the development of interspecific and intergeneric hybrids in sexually incompatible species of $Brassica$. Crop improvement using somaclonal variation has also been achieved. Transformation technologies are being exploited routinely to elucidate the gene function and contribute to the development of novel enhanced crops. The $Agrobacterium$-mediated transformation is the most widely used approach for the introduction of transgenes into Brassicaceae, and $in$ $vitro$ regeneration is a key factor in developing an efficient transformation method in plants. Although many other Brassicaceae are used as model species for improving plant regeneration and transformation systems, this paper focuses on the recent technologies used to regenerate the most important Brassicaceae crop plants.

• 한국가구학회지
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• 제25권4호
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• pp.304-318
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• 2014

Bed for elderly and disabled, including the United States developed the industry Americas, and European countries, mainly in the production of products has dominated the world market. In large hospitals, and dependent products are sold at high prices. On the other hand, improvement of living standards of our country and to the advancement of residential culture of the demand for luxury products is increasing. The study of elderly Edition mattresses have been developed mainly in Europe to maximize the use of sleep can increase the efficiency of products has been studied. Due to the increase in elderly population Elderly Hospital, which is installed in the elderly silver town Edition requires the dissemination of the mattress in the bedroom several times a day resting and sleeping, eating, etc. Designing a variety of actions are needed. Edition of the elderly in Korea developed the design of bed with bed design and the manufacturer and the Institute for Ergonomics, welfare agencies and to build organic cooperation system under the direction of a professional designer to develop and you need to configure the space and the results of such actions by the distribution of cheap materials, ergonomic mattress for optimal development of behavior analysis to the need to promote the convenience of the user.

• 대한미생물학회지
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• 제22권3호
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• pp.197-208
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• 1987

Chlamydia trachomatis has now shown that this interesting intracellular parasite is a cause of nongonococcal urethritis, infantile pneumonia, pelvic inflammatory disease and epididymitis, in addition to lymphogranuloma venerum and inclusion conjunctivitis. There are several diagnostic methods for C. trachomatis, but the method using monoclonal antibody is the most sensitive and specific. The hybride cell were prepared by fusion of myeloma cell($P_3X_{63}\;Ag_8{\cdot}V_{653}$) of mouse and lymphocyte of mouse(BALB/c) that were immunized with formalin killed C. trachomatis serotype D. The cell mixtures after fusion were dispensed into 640 wells of the 96 well culture plates and continuously cultured in HAT medium for 2 weeks. The supernatants of culture media in 83(13%) wells were reacted with C. trachomatis, which were determined by enzyme-linked immunosorbent assay in 96 well microplate. The clones that secreted antibody to C. trachomatis were cloned by limiting dilution. Only six monoclones secreted antibody to C. trachomatis. The antibody titer of ascitic fluid that collected from same BALB/c mice bearing hybridoma cells was above 1:100,000. These monoclonal antibodies that were IgG reacted with elementary and reticulate bodies of all serotypes(Ba, D, E, F, G, H, J and LGV type-I) using ELISA and indirect immunofluorescence stain, but there were no cross reaction with other bacteria(coagulase negative Staphylococcus, Proteus and E. coli). We concluded these six monoclones secreted the same monoclonal antibody to C. trachomatis. The sensitivity and specificity of the monoclonal antibody compared with Microtrak(confirmatory test of C. trachomatis, Syva) was 100%, respectively.

• 문화기술의 융합
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• 제6권2호
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• pp.263-270
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• 2020

최근 여러 학문 분야와의 협력과 통합을 통한 새로운 지식의 창출과 창의적 문제 해결 능력을 갖출 수 있는 교육의 필요성이 증가됨에 따라 대학에서는 타 전공지식과 소프트웨어 소양을 겸비한 창의융합인재 양성을 위해 소프트웨어 역량 교육의 필수화를 추진하고 있다. 컴퓨터 비전공자를 대상으로 하는 소프트웨어 교육을 통해 창의융합인재를 양성하기 위해서는 학습자가 다양한 지식을 활용 및 적용하여 문제를 스스로 해결 할 수 있는 능력을 키우는 것이 중요하다. 따라서 본 연구에서는 컴퓨터 비전공 대학 신입생들이 소프트웨어 교과목을 이수하며 만들어낸 프로젝트와 학습자의 사전 사후 설문조사 결과를 분석해봄으로써 소프트웨어와 학습자 전공분야와의 융합 가능성을 탐색하고 향후 컴퓨터 비전공 대학 신입생들의 소프트웨어 교육 방향에 대해 제언하고자 한다.

• 한국초지조사료학회지
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• 제15권1호
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• pp.1-12
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• 1995

This study was to investigate an effective biological control of forage diseases and provide a basic data and a model in improving variety of antagonistic bacteria, with growth promoting effect on forage, through cell fusion. The results obtained were summarized as follows; 1. The antagonistic himbacterium against soil-borne phathogenic fungi Fusarium oxysporum and Rhizoctonia solani was isolated from continuous cropping himsphere soil of forage, and its biological and physiological characteristics were investigated. This bacterium was identified as Bacillus subrilis and named BS 101. Another strain for cell fusion was Bacillus thur ingiensis ssp. kurstaki HD-I(BT 37669) with insecticidal crystal. 2. The auxotropic mutants of BS 101 and BT 37669 were derived after mutagenesis using N-methyl-N'nitro- Nitrosoguanidine(NTG) to give amino acid requirement marker. n e s e auxotropic mutants of BS 101 and BT 37669 were named BS 1013(his-) and BT 69(asp-), respectively. 3. The best protoplast requirement was obtained using DM 3 medium, containing 5% casamino acid, 1 M $MgCI_2$ and 2% bovine semm albumin, to give Fusant 3, 7 and 8. BT toxin gene was not identified with fusants by Southern blotting. However, SDS-PAGE analysis of strains showed various protein patterns among fusants. 4. From the dark culture experiment, growth of forage in inoculated soil with antagonistic bacteria was delayed than that of non-inoculated soil with antagonistic bacteria in each continuous cropping soil and in each sterilized soil. On the other hand, growth duration of forage was different between continuous cropping soil and sterilized soil. 5. Seed germination of Alfalfa, Italian ryegrass and Orchardgrass were significantly improved by inoculation of antagonistic bacteria(p< 0.05).

• 통상정보연구
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• 제13권2호
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• pp.151-176
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• 2011

본 연구는 한국의 중재현황을 살펴본 뒤 최근의 학문적 추세를 고려하여 학문융합적 연구방법을 통하여 중재를 연구하는 것이 미래 중재연구방향에 있어 중요함을 밝히고 있다. 한국의 중재현황을 살펴보면, 첫째는 한국의 교역규모와 교역상대국이 증가함에 따라서 분쟁이 증가하면서 중재가 중요한 분쟁해결방식으로 부상하고 있다는 점이다. 둘째, 분쟁의 원인을 살펴보면 대금결제와 매매계약과 관련된 분쟁이 가장 큰 원인이라는 점이다. 셋째, 분쟁당사국들도 유럽을 포함해서 수가 늘어나고 있다는 점이다. 이러한 특정들은 중재를 하나의 학문적 방법론에만 의지해서 연구하는 것이 충분치 않다는 점을 나타내고 있다. 본 연구에서는 학문융합적 중재연구프로그램을 제시하였다. 본 연구는 학문융합적 방법론으로 법학적 방법론, 제도적 방법론, 문화적 방법론, 인과적 방법론, 실증적 방법론을 제시하였으며, 이러한 방법론들을 중재에 도입하여 중재를 학문 융합적으로 분석할 것을 제안하였다. 전통적으로는 법학적 방법론이 중재연구에서 주로 사용한 방법론이었지만 향후 중재는 그 외의 다양한 방법론을 통해서 연구하는 것이 바람직해 보인다.

• 복식
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• 제57권3호
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• pp.176-190
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• 2007

As the globalization has increasingly brought in the disintrgration of boundary between cultures, the hybrid of styles, or fusion styles in various cultural spheres have been introduced as important theme. With this new trend, the traditional culture of Asia appeared as the source of inspiration for the West, and as the source of enhanced pride and asset for ethnic groups which have been considered "the Other" by the West. 1990's witnessed a drastically increased interest in Orientalism and Ethnic trends in most social aspects, especially in culture and art. They have been the main theme in fashion, providing the source of inspiration with elements such as the unique color schemes, composition methods and geometrical simplicity. The creative application of traditional culture into modern design as well as fashion can make a significant contribution and be a solid foundation for the development of national culture in general, since images containing cultural authenticity are the visual representation of the nation and they can be important tools for the globalization of design. This study aims to find out the formative characteristics of Korean traditional embroidery and the ways they are applied in modern fashion by world-renowned Korean fashion designers. The purpose of this study is to make a fundamental source for further study by the same author on creative design development utilizing the result. The study methods are literature study combined with research of genuine articles from museums and personal ownership as well as photos from magazines and internet. The significance of this study lies in enhancing the appreciation of Korean traditional culture and expanding the possibility of its globalization by modern application.

• 복식문화연구
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• 제15권1호
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• pp.127-136
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• 2007

Modern society shares new culture derived from the fusion of various cultures unlike the past culture regulating one phenomenon of certain field as the representative icon of the era. Especially, the phenomenon of globalization acts as the catalytic agent to receive coexistence of variousness easily. Especially, in fashion industry, these flows and the view of naturalism are harmonized and new fashion trend called 'Ethnic' was embossed. As it reflects internal spirit of human being that wants to return to the memory due to expansion of material civilization, the interest in the designer pursuing it is increasing. This study tries to consider the Ethnic style displayed by Chinese American designer, Anna Sui, who pursues the eastern style with full wit and individuality. Anna Sui, who was praised by New York Times for 'the superb harmony of houte couture style and up-to-date style', created another fashion trend, romanticism in New York and she is the worldwide designer representing Ethnic. Like it, the consideration on her Ethnic style reanalyzing as eastern style provides guidance to check how designer's fashion philosophy appears in one category of America and China and new study material that enables us to understand the third world culture code as well as Anna Sui. This confirmation has the meaning as the basic material for the endless challenge to the new style that fashion will go on in the future. Anna Sui was confirmed to have been recognized as a designer who represented the ethnic collection in the United States, exerting the influence with her unique oriental background. Moreover, her design could identify the pivotal point of ethnic image by developing her unique style which is distinct from other designers in terms of silhouette, color, fabric, detail, and so on. This kind of study can provide the basic data required to understand the fashion world of designer in the future.

• 한국식생활문화학회지
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• 제22권6호
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• pp.794-800
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• 2007

The purpose of this study was to measure the tourists' preference for alternative restaurants with different combinations of 4 attribute levels: origin description, food type, price and service guarantee. A total of 210 questionnaires were completed from tourists who visited Kwangyang, Suncheon and Yeosu during Jan. 2 - Jan. 15, 2007. Conjoint experiment method was used to develop hypothetical restaurants. Ordinal probit model was used to measure the effects of attribute levels on the tourists' preference. Results of the study demonstrated that the ordinal probit model analysis result for the data indicated excellent model fit. The effects of attribute levels (origin description, traditional food, fusion food, price, service guarantee) on the tourists' preference were statistically significant. As expected, estimates of marginal willingness to pay for origin description(3.063), food type(2.349), and service guarantee(2.356) were statistically significant. Moreover, tourists were more willing to pay for origin description than other attribute levels. Tourists also considered the origin description as the very important attribute. In conclusion, based on conjoint analysis, a model was proposed of marginal willingness to pay of attribute levels. It should be noted that the original model was modified and should, preferably, be validated in future research.

• 복식문화연구
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• 제13권5호
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• pp.777-789
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• 2005

Mix & Match Visual Image, appearing in T-shirt one-point graphics, is a new visual image emerged as a result of several trends: development of cutting-edge machinery and computer programs, various needs of consumers, and an international trend of fusion concept. Thus, a concrete research of Mix & Match Visual Images is demanding. This paper studies one-point graphic, which appeared in the SamsungDesignNet, Interfashion Planning, and firstviewkorea, with a focus on apparel T-shirt of women's wear and casual wear from 2001 to 2004. The study shows that the most preferred independent visual image in Mix & Match Visual Images is a typography, and next is in the order of marks, symbols, geometric diagrams and abstract shapes, animals and plants, characters, materials, and collages. As Mix & Match of independent visual images was mainly combined typography with other independent visual images, we need to develop more Mix & Match Visual Images using various independent visual images's mix and match. Then, T-shirt will be one of the most representative items in Korean fashion trend that enable consumers to express their characters, escaping from the old-fashioned concept that T-shirt is just a T-shirt.