• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.5
• /
• pp.320-326
• /
• 2000

Promoters of the genes G3P, ICL1, POT1, POX1, POX2 and POX5 of the yeast Y. lipolytica were studied in respect to their regulations and activities during growth on different carbon sources. The aim of this study was to select suitable promoters for high expression of heterologous genes in this yeast. For this purpose the promoters were fused with the reporter gene lacZ of E. coli and integrated as single copies into the genome of Y. lipolytica strain PO1d. The measurement of expressed activities of ${\beta}$-galactosidase revealed that pICL1, pPOX2 and pPOT1 are the strongest regulable promoters available for Y. lipolytica, at present. pPOX2 and pPOT1 were highly induced during growth on oleic acid and were completely repressed by glucose and glycerol. pICL1 was strongly inducible by ethanol besides alkanes and fatty acids, however, not completely repressible by glucose or glycerol. Ricinoleic acid methyl ester appeared as a very strong inducer for pPOT1 and pPOX2, in spite of that it inhibited growth of Y. lipolytica transformants.

• Computers and Concrete
• /
• v.3 no.4
• /
• pp.197-212
• /
• 2006

Modeling of physically non-linear behavior becomes more and more important for the analysis of SFRC structures in practical applications. From this point of view we will present an effective, three-dimensional constitutive model for SFRC, that is also easy to implement in commercial finite element programs. Additionally, the finite element analysis should only require standard material parameters which can be gained easily from conventional experiments or which are specified in appropriate building codes. Another important point is attaining the material parameters from experimental data. The procedures to determine the material parameters proposed in appropriate codes seem to be only approximations and are unsuitable for precise structural analysis. Therefore a finite element analysis of the test itself is used to get the material parameters. This process is also denoted as inverse analysis. The efficiency of the proposed constitutive model is demonstrated on the basis of numerical examples and their comparison to experimental results. In the framework of material parameter identification the idea of a new, indirect tension testing procedure, the "Modified Tension Test", is adopted and extended to an easy-to-carry-out tension test for steel fiber reinforced concrete specimens.

• Journal of Family Resource Management and Policy Review
• /
• v.10 no.4
• /
• pp.65-85
• /
• 2006

The purposes of this study are to classify housewives based on their perception of benefits sought of preschool child education commodity and to identify the characteristics of each type. For empirical analysis, the data for this study were collected from 400 full-time housewives with preschool child living in Seoul. The statistical methods adopted fur data analysis are cluster analysis, one-way ANOVA, Duncan's multiple range test and discriminant analysis. The major findings of this study are as follows : First, four housewives' types can be categorized by the level of those 3 dimensions. The name fur the types was given as 'sticking to one's belief type', 'taking importance to early stimulation type' 'conforming to and imitating others type', and 'believing blindly in education commodity type'. Second, the result of discriminant analysis shows that 'housewives' education level and their education-related values' element was the most crucial in classifying the housewives, and 'considering child's characteristics and educational home environment' element was the next. The results of this study suggest several implications fur the education of parents with preschool child. Parents' education programs differentiated by the objects and the problems should be developed : which are suitable for each housewives' type.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.28 no.4
• /
• pp.378-385
• /
• 2004

Viscous-driven pumping is a very promising type in microscale applications. However, there exist a few disadvantages such as low efficiency and small volume flow rate. In the present study, a pump with tandem rotating cylinders and its optimum synthesis are proposed fur enhancing pumping performance. First, using an unstructured grid CFD method, we investigate the effects of geometrical parameters and then the performance of the pump with tandem cylinders is evaluated. Next, an optimum design synthesis tool is constructed by combining the aforementioned CFD analysis model with the mathematical optimization model, namely, Modified Method of Feasible Directions (MMFD). This technique is used to optimize the geometrical parameters of the pump, fur maximizing pumping efficiency. From the optimization results, it is believed that the present optimum synthesis is robust and has a potential fur other microfluidic device design.

• Journal of Animal Science and Technology
• /
• v.57 no.2
• /
• pp.6.1-6.5
• /
• 2015

The objectives of this study were to study the effects of different levels of dietary copper on organ indexes, tissular Cu, Zn and Fe deposition and fur quality of mink in the growing-furring periods. One hundred and five standard dark male mink were randomly assigned to seven groups with the following dietary treatments: basal diet with no supplemental Cu (Control); basal diet supplemented with either 6, 12, 24, 48, 96 and 192 mg/kg Cu from copper sulphate, respectively. The colour intensity scores displayed a linear trend (P = 0.057). The spleen Cu concentrations responded in a linear (P < 0.05) fashion with increasing level of Cu, but copper supplementation did not affect speen concentrations of Fe or Zn. Supplemental dose of Cu linearly increased (P < 0.05) liver Cu and Fe concentrations but did not alter (P > 0.10) liver Zn. Our results indicate that Cu plays an important role in the pigmentation in growing-furring mink, and supplemental dietary Cu in growing-furring mink improve hair colour, and copper has limited effects on liver mineral deposition.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.26 no.10
• /
• pp.1961-1970
• /
• 2002

A laser scanner is widely used fur a device fur acquiring point data in reverse engineering. It is more efficient to generate a surface automatically from the line-typed data than scattered data of points clouds. In the case of a compound model, it is hard to represent all the scanned data into one surface maintaining its original line characteristics. In this paper, a method is presented to generate a surface by the segmentation of measured point data. After forming triangular net, the segmentation is done by the user input such as the angle between triangles, the number of facets to be considered as small segment, and the angle for combining small segment. B-spline fitting is implemented to the point data in each segment. The surface generation through segmentation shows a reliable result when it is applied to the models with curvature deviation regions. An useful algorithm for surface reconstruction is developed and verified by applying an practical model and shows a good tools fur reverse engineering in design modification.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2005.10a
• /
• pp.415-418
• /
• 2005

In the semiconductor and optical industry, a new transport system which can replace the conventional transport systems is required. The transport systems are driven by the magnetic field and conveyer belts. The magnetic field may damage semiconductor and the contact force may scratch the optical lens. The ultrasonic wave driven system can solve these problems. In this semiconductor and optical industry, the non-contact system is required fur reducing the damages. The ultrasonic transportation is the solution of the problem. In this paper, the ultrasonic levitation system fur levitating object are proposed. The 3D vibration profiles of the beam are measured by Laser scanning Vibrometer fur verifying the vibration characteristics of the system and the amplitudes of the beam and the levitation heights of object are measured for evaluating the performance.

• International Journal of Korean Welding Society
• /
• v.2 no.2
• /
• pp.7-13
• /
• 2002

A comparison of the welding performance of ship hull structural steels has been made. The weldability of steels especially designed for laser processing was compared to that of conventional hull and structural steels with plate thicknesses up to 12 mm. Autogenous laser beam welding was used to weld butt joints as well as skid and stake welded T-joints. The welds were assessed in accordance with the document "The Classification Societies" Requirements for Approval of $CO_2$ Laser Welding Procedures" Small imperfections in the weld only grew slightly in root bend tests and they only had a minor influence on the fatigue properties of laser fillet welded joints. In Charpy impact tests, the 27 J transition temperature of the weld metal and HAZ ranged from below -60 to $-50^{\circ}C$. The amount of martensite in the weld metal depended on the carbon equivalent of the steel with the highest amounts and highest hardness levels in conventional EH 36 (389 HV 5). Thermomechanically rolled steels contained less martensite and showed a correspondingly lower maximum hardness.ximum hardness.

• Proceedings of the KWS Conference
• /
• 2002.10a
• /
• pp.222-228
• /
• 2002

A comparison of the welding performance of ship hull structural steels has been made. The weldability of steels especially designed for laser processing was compared to that of conventional hull and structural steels with plate thicknesses up to 12 mm. Autogenous laser beam welding was used to weld butt joints as well as skid and stake welded T-joints. The welds were assessed in accordance with the document "The Classification Societies′ Requirements for Approval of $CO_2$ Laser Welding Procedures". Small imperfections in the weld only grew slightly in root bend tests and they only had a minor influence on the fatigue properties of laser fillet welded joints. In Charpy impact tests, the 27 J transition temperature of the weld metal and HAZ ranged from below -60 to -5$0^{\circ}C$. The amount of martensite in the weld metal depended on the carbon equivalent of the steel with the highest amounts and highest hardness levels in conventional EH 36 (389 HV 5). Thermomechanically rolled steels contained less martensite and showed a correspondingly lower maximum hardness.

• Nuclear Engineering and Technology
• /
• v.13 no.4
• /
• pp.207-220
• /
• 1981

The Np-237 fission cross-sections up to 20 MeV incident neutron energy are calculated by means of the computer code STAPRE- a statistical model code with consideration of preequilibrium decay. The higher chance fissions up to third compound nucleus are taken into account, and the main input parameters in the treatment of fission under consideration of a double-humped fission barrier are carefully adjusted, so that the current trend of experimental data can be fitted within an apparent deviation of about 10% throughout the entire energy range. Results are presented in the form of point-wise cross-section values, and also in the form of graph to demonstrate the shape agreement.