• Title/Summary/Keyword: fungi sp

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Enhancing Effect of Shimizuomyces paradoxus on Seed Germination and Seedling Growth of Canola, Plant Growth of Cucumber, and Harvest of Tomato

  • Sung, Gi-Ho;Shrestha, Bhushan;Park, Ki-Byung;Han, Sang-Kuk;Sung, Jae-Mo
    • Mycobiology
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    • v.39 no.1
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    • pp.7-11
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    • 2011
  • Shimizuomyces paradoxus showed no inhibitory effect against plant pathogen fungi, such as Fusarium oxysporum f. sp. lycopersici and Alternaria solani. The S. paradoxus culture filtrate showed higher seed germination and seedling growth rates in canola than distilled water and potato-dextrose broth. A conidial suspension of $1.0{\times}10^4/mL$ resulted in the highest growth stimulating effects on total plant length, and fresh and dry weight of shoots and roots in cucumber, when compared to the highest suspension concentration. Total plant length and shoot weight increased with the foliar spray treatment, and root length and root weight increased by simultaneous treatments of soil drenching and foliar spray in cucumber. Lower concentrations of the S. paradoxus conidial suspension increased the harvest of tomato fruit.

Selection and Antifungal Activity of Antagonistic Bacterium Pseudomonas sp. 2112 against Red-Pepper Rotting Phytophthora capsici (생물방제균 Pseduomonas fluorescens 2112의 선발과 고추역병균에 대한 항진균성 길항작용)

  • 이은탁;김상달
    • Microbiology and Biotechnology Letters
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    • v.28 no.6
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    • pp.334-340
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    • 2000
  • In order to select multifunctional powerful antagonistic biocontrol agent against red-pepper rotting fungi Phytophthora capsici, we isolated an indigenous antagonistic bacterium which produces antifungal substances and siderophores from a local soil of Kyongju, Korea. The isolated strain was identified as Pseudomonas fluorescens biotype F. The antibiotic produced from P. fluorescens 2112 inhibited hyphae growth and the zoospore germination of Phytophthora capsici. The favorable carbon, nitrogen source and salts for the production of antibiotic from P. fluorescens 2112 were glycerol, beef extract and LiCi at 1.0%, 0.5% and 5 mM, respectively. And antagonistic activity of P. fluorescens 2112 was confirmed against P. capsici in vivo.

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Evaluation of Antimicrobial Activity of Farnesoic Acid Derivatives

  • Kim, Sang-Hee;Oh, Ki-Bong
    • Journal of Microbiology and Biotechnology
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    • v.12 no.6
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    • pp.1006-1009
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    • 2002
  • The biological activities of farnesoic acid derivatives against pathogenic fungi and bacteria were investigated. Farnesoic acid and its derivatives showed growth inhibitory activities against various bacteria. Among the compounds tested, geranylgeranoic acid (3) had potent antibacterial activity against Salmonella typhimurium, Proteus vulgaris, and Bacillus subtilis with minimum inhibitory concentration (MIC) in the range of $6.25-12.5{\mu}g/ml$. On the other hand, amide derivatives of farnesoic acid showed some antifungal activities. In particular, 3,7,11-trimethyl-dodeca-2,6,10-trienoic acid amide (5a) had a potent antifungal activity against Aspergillus niger, Candida albicans, and Trichophyton sp. with MIC in the range of $6.25-12.5{\mu}g/ml$.

Control of Aflatoxin Production of Aspergillus flavus by Inghbitory Action of Antagonistic Bacteria

  • Cho, Jung-Il;Hong, Kwang-Won;Kang, Kil-Jin
    • Journal of Microbiology and Biotechnology
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    • v.10 no.2
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    • pp.154-160
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    • 2000
  • Aflatoxin B1 is known as the most potent mycotoxin produced by several fungi. It has been demonstrated to be not only carcinogenic but teratogenic and mutagenic as well in humans. To prevent or inactivate aflatoxins, several chemical of physical methods were tested for ammoniation, using insecticides as an wxample, but they were unsuitable for food products. On the contrary, biological control by antagonistic microorgani는 is and ideal method. In order to control aflatoxin B1 biologically, the antagonists #07, #63, #75, #74, and #61 were separated from various samples by using the antagonistic activity test. Among them, culture filtrate part A (non heat-treated) of #63 and #74 on aflatoxin B1 produced by Aspergillus fkavus were shown to be 95% and 75%, respectively. Based on the morphological characteristics, #63 was deduced as an Azospirillum sp.

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Endophytic fungi harbored in Chinese native gramineous plants

  • Wang, Zhiwei;Ji, Yanling
    • 한국균학회소식:학술대회논문집
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    • 2009.10a
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    • pp.44-56
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    • 2009
  • $Epichlo\ddot{e}$ endophytes, including Neotyphodium spp. and $Epichlo\ddot{e}$ spp., enhance plant growth, mediate more plant tolerance or resistance to biotic and abiotic stresses, and also synthesis various biologically active compounds in their host plants, and important in many areas. In early stages, most of $epichlo\ddot{e}$ endophytes were described during surveys practiced by American, European and Oceania scientists, while fungal endophytes within native Asian plants were poorly investigated. In recent years, an $Epichlo\ddot{e}$ sp. and 4 Neotyphodium spp. were described in cool season Chinese native gramineous plants. Most of Chinese native Neotyphodium spp. were presumed as hybrids originated from members of ETC and EBY. Investigation on NRPS genes shows lack of toxic ergopeptines and potential production of peramine. Biological and ecological roles of Chinese native $epichlo\ddot{e}$ endophytes should be investigated in future, and it will be very valuable if we can have some joint projects with Korean scientists for Asian native $epichlo\ddot{e}$ endophytes.

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Screening of Deoxynivalenol Producing Strains from Agricultural Products by Immunoanalytical Method (면역분석기법에 의한 농산물에서의 Deoxynivalenol생성균 검색)

  • 강성조;오상석;박정현;김형갑;장덕화
    • Journal of Environmental Health Sciences
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    • v.27 no.4
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    • pp.35-40
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    • 2001
  • In order to evaluate the safety of agricultural products in Korea, we carried out work by screening of Fusarium species. which can produce deoxinivalenol(DON) from agricultural products in Western Gyeongnam, Korea. From 215 samples of soil agricultural products, 129 strains of Fusarium species were obtained. The production of DON was verified by thin layer chromatography(TLC) As the results of TLC, 25 strains were identified as DON producing strain. But only 10 strains were identified as DON producing strains by enzyme-linked immunosorbent assay (ELISA). The maximum DON producing strain No.41 was isolated from corn. In conclusion. the above results indicate that DON producing fungi contaminated agricultural products in Korea. Therefore further studies are required to accumulate more detailed data about the contamination of DON in various agricultural products.

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Isolation and Identification of a Soil Actinomycetes YBE-316 Producing an Antitumor Antibiotic (항암성 항생물질을 생산하는 토양 방선균 YBE-316의 분리 및 동정)

  • Shin, Jin-E;Park, Jae-Hong;Bai, Dong-Hoon;Yu, Ju-Hyun
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.297-303
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    • 1995
  • Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

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Purification and Characterization of Extracellular $\beta$-Xylosidase from Fungi (곰팡이가 생산하는 세포외 $\beta$-Xylosidase의 정제 및 특성)

  • 고명선;이상준;이종근
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.627-635
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    • 1994
  • The $\beta$-xylosidase from Penicillium sp. FX-102 was purified by 40~80% ammonium sulfate saturation, CM-Cellulose column chromatography, Sephadex G-200 gel filtration, and isoelec- tric focusing. The optimum pH and temperature for the activity of the $\beta$-xylosidase was pH 4.5 and 50$\circ$C, respectively. The enzyme was stable at the pH range of 4.5~5.5, and at 55$\circ$C for 10 min. The molecular weight of the enzyme was estimated to be about 300,000 daltons by Sephadex G-200 gel filtration and 310,000 daltons of monomer by SDS polyacrylamide gel electrophoresis. Isoelectric point of the enzyme was determined to be pH 4.4. The enzyme activity was strongly inhibited by Hg$^{2+}$, Ag$^{2+}$, n-bromosuccinimide and p-chloromercuribenzoate. Xylobiose (10 mM) was completely decomposed to xylose after 8 hrs enzyme reaction with 2 units of the $\beta$-xylosidase.

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가칭 Yucca의 운문병

  • 이구영
    • Journal of Plant Biology
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    • v.4 no.1
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    • pp.21-24
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    • 1961
  • The strains of fungus which belong to Coniothyrium genus were isolated from leaf spoot of Yucca. The isolated strain can grow easily and forms characteristic colonies on Saido's soy agar. There are definite differences, according to other authors, (By Miyake et Hara 1911) Pycnidio-spore are usually formed ellipsoidal, dark brown, 4$\times$2. 5-4. $O\mu$ in size, whereas isolated strains are globose, yellowish brown, 2.8-4.2$\mu$ in diameter. The positive inoculation test can be attained by burned petridish method, preserving it in incubator at 28$^{\circ}C$, and the pycnidio-spore can be reisolated from the inoculated portion. (Photo.7) Up to the present day there is no report for leaf spot of Yucca caused by Coniothyrium sp.. Comparing the writer's isolate with those fungi described(leaf spot of alloe, rose canker and elm canker etc.) hitherto, there are no accordance in the symptom and especially in morphological charactgers and Pathogenicitys.

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The List of the Fungi of Korea (한국균심목록)

  • Lee, Ji-Yul
    • The Korean Journal of Mycology
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    • v.1 no.1
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    • pp.35-43
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    • 1973
  • 저자(著者)는 우리나라산(産) 균심류의 정체(正體)를 구명(究明)하고자 1946년(年)부터 금일(今日)에 이르는 동안 서울시내(市內), 광릉(光陵), 수원(水原), 양양(襄陽), 속초(束草), 설악산(雪嶽山), 목포(木浦), 나주(羅州), 울산반도등지(蔚山半島等地)의 산야(山野) 인가(人家) 노변(路邊)에서 야생균심(野生)을 채집정리(採集整理)하여 기(旣)히 조사발표(調査發表)된 종(種)을 종합(綜合)하여 2문(門), 2강(綱), 3목(目), 12아목(亞目), 22과(科)7아과(亞料), 274종(種)을 보고(報告)하는 바이다. 저자(著者)가 금일(今日)까지 미기록종(未記錄種)으로 사정(査定)한 종(種)은 하기(下記)와 같다. 1. Polysticus flabelliformis (Klotzsch.) Cke. 2. Thelephora palmata (Scopoli) Fr. 3. Russula nigricans (Bull.) Fr. 4. Omphalia sp. 5. Peziza repanda Pers. 6. Wynnea gigantea Berk. et Curt. 7. Helvella crispa (Scop.) Fr. 8. Helvella epipploides Imai 9. Cordyceps militaris (L.) Link

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