• Title/Summary/Keyword: fungi sp

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Harmful Microorganisms Occurred on the Bed-logs of Several Quercus spp. for Shiitake Cultivation (표고재배시 여러 종류의 참나무골목에 발생되는 해균상)

  • Park, Won-Chull;Ka, Kang-Hyeon;Yoon, Kab-Hee;Ryu, Sung-Ryul;Lee, Bong-Hun
    • The Korean Journal of Mycology
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    • v.35 no.1
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    • pp.33-36
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    • 2007
  • An attempt was made to investigate the status of harmful microorganisms occurring on different kinds of oak bed-logs during shiitake cultivation. As a result, totally 14 species of harmful microorganisms, including Trametes versicolor, were confirmed. Twelve kinds of harmful microorganisms were observed on Quercus acutissima, 9 kinds on Q. mongolica and 10 kinds on Q. aliena. Diatrype stigma, Hypoxylon truncatum, Hypoxylon sp. and Trichoderma sp. occupied 75.1% of the total harmful fungi occurred on Q. acutissima. H. truncatum and Trichoderma sp. occupied 71.2% of the total harmful fungi occurred on Q. mongolica. On Q. aliena, the occurrence of H. truncatum, Trichoderma sp. and Hypoxylon howeianum was 80.3%. D. stigma and Hypoxylon sp. were observed exclusively on Q. acutissima bed-logs, and the outbreak ratios were 51.6% and 13.1%, respectively. H. truncatum was observed on 46.6% of Q. aliena bed-logs and Trichoderma sp. was observed on 30.3% of Q. mongolica bed-logs.

Characterization and Antifungal Activity from Soilborne Streptomyces sp. AM50 towards Major Plant Pathogens

  • Jang, Jong-Ok;Lee, Jung-Bok;Kim, Beam-Soo;Kang, Sun-Chul;Hwang, Cher-Won;Shin, Kee-Sun;Kwon, Gi-Seok
    • Korean Journal of Environmental Agriculture
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    • v.30 no.3
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    • pp.346-356
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    • 2011
  • BACKGROUND: Chemical fungicides not only may pollute the ecosystem but also can be environmentally hazardous, as the chemicals accumulate in soil. Biological control is a frequently-used environment-friendly alternative to chemical pesticides in phytopathogen management. However, the use of microbial products as fungicides has limitations. This study isolated and characterized a three-antifungal-enzyme (chitinase, cellulase, and ${\beta}$-1,3-glucanase)-producing bacterium, and examined the conditions required to optimize the production of the antifungal enzymes. METHOD AND RESULTS: The antifungal enzymes chitinase, cellulase, and ${\beta}$-1,3-glucanase were produced by bacteria isolated from an sawmill in Korea. Based on the 16S ribosomal DNA sequence analysis, the bacterial strain AM50 was identical to Streptomyces sp. And their antifungal activity was optimized when Streptomyces sp. AM50 was grown aerobically in a medium composed of 0.4% chitin, 0.4% starch, 0.2% ammonium sulfate, 0.11% $Na_2HPO_4$, 0.07% $KH_2PO_4$, 0.0001% $MgSO_4$, and 0.0001% $MnSO_4$ at $30^{\circ}C$. A culture broth of Streptomyces sp. AM50 showed antifungal activity towards the hyphae of plant pathogenic fungi, including hyphae swelling and lysis in P. capsici, factors that may contribute to its suppression of plant pathogenic fungi. CONCLUSION(S): This study demonstrated the multiantifungal enzyme production by Streptomyces sp. AM50 for the biological control of major plant pathogens. Further studies will investigate the synergistic effect, to the growth regulations by biogenic amines and antifungal enzyme gene promoter.

Numerical Identification of Streptomyces fIaveus Producing Antibiotic Substances Inhibitory to Plant Pathogenic Fungi

  • Lee, Jung-Yeop;Kim, Beom-Seok;Hwang, Byung-Kook
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.324-334
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    • 1995
  • The actinomycete strain A 11 was antagonistic to plant pathogenic fungi Phytophthora capsid and Magnaporthe grisea. Based on the diaminopimelic acid (DAP) type and morphological characteristics examined by scanning electron microscopy, the strain A 11 was confirmed to belong to the genus Streptomyces. Based on Willcox probability and similarity level, the strain A 11 was numerically identified as Streptomyces flaveus using TAXON program of Ward and Goodfellow. Antibiotic production of S. flaveus strain A 11 was most favorable when cultured on glycerol yeast extract peptone (GYP) agar for 20 days at $28^{\circ}C$. The crude antibiotics from solid GYP agar cultures of the strain A 11 were most effective against Phytophthora capsici and Sclerotinia sclerotiorum among the fungi tested. Antifungal activity of the antibiotics against Alternaria solani, Botryosphaeria dothidea, Cercospora capsici, Magnaporthe grisea, and Rhizoctonia solani was somewhat high, whereas Colletotrichum gloeosporioides and Fusarium oxysporum f. sp. cucumerinum were rarely inhibited even at high concentrations.

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Effect of 1-corydalmine, an Alkaloid Isolated from Corydalis chaerophylla Roots on Spore Germination of Some Fungi

  • Basha, S. Ameer;Jha, R.N.;Pandey, V.B.;Singh, U.P.
    • Mycobiology
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    • v.35 no.2
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    • pp.69-71
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    • 2007
  • 1-Corydalmine, an alkaloid isolated from roots of Corydalis ehaerophylla inhibited spore germination of some plant pathogenic as well as saprophytic fungi e.g. Alternaria brassicae, A. brassicieola, A. solani, Curvularia lunata, C. maculans, C. sp., C. pallseens, Erysiphe pisi, Fusarium udum, Helminthosporium species, H. penniseti and a Heterosporium species. 1-Corydalmine significantly inhibited spore germination of all the fungi at 100 to 1500 ppm. It was effective against all the fungi at 1500 ppm. C. lunata was highly sensitive to this chemical even at 250 ppm.

Isolation of a Wood-rotting Fungus to Decolorize a Wide Range of Structurally Different Synthetic Dyes. (다양한 염료의 탈색이 가능한 목재부후균 분리)

    • Microbiology and Biotechnology Letters
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    • v.31 no.3
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    • pp.301-306
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    • 2003
  • Twenty-one different fungi were tested for their ability to decolorize a wide range of structurally different dyes. Twenty fungal strains were isolated from fruiting bodies which were collected at the Kwangneung National Arboretum, Korea. One fungal strain were isolated from a rotting wood at Soongsil University, Korea. Nine kinds of dyes were used: three anthraquinone dyes and six azo dyes. The five fungal strains, Laetiporus sulphureus, Polyporus arcularius. Auricularia polytricha, Stereum ostrea, and Bjerkandera sp. UK-l showed decolorization ability. Except Auricularia polytricha, the four fungal strains were wood rotting fungi, and belonged to Aphyllophorales. Bjerkandera sp. UK-I, which was a white rot fungus, could decolorize all kinds of dyes tested in this study, indicating this fungus is one of candidates for applying in biological methods of dye waste treatment.

Occurrence of Sooty Blotch and Flyspeck Disease on Sweet Persimmon in Korea

  • Kwon, Jin-Hyeuk;Kim, Jin-Woo;Choi, Ok-Hee;Gang, Guen-Hye;Kwak, Youn-Sig
    • Mycobiology
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    • v.40 no.3
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    • pp.210-213
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    • 2012
  • Sooty blotch and flyspeck (SBFS), a disease caused by a complex of fungi, results in substantial economic losses for commercial growers of sweet persimmon (Diospyros kaki L.) in Korea. However, many species causing SBFS in Korea have not been identified and sources of inoculum are uncertain. Based on mycological characteristics, pathogenicity, and molecular data, the causal fungi were identified as Dissoconium sp. and Zygophiala wisconsinensis. This is the first report of SBFS of sweet persimmon in Korea.

Potential of Endophytic Diaporthe sp. as a New Source of Bioactive Compounds

  • Nagarajan, Kashvintha;Tong, Woei-Yenn;Leong, Chean-Ring;Tan, Wen-Nee
    • Journal of Microbiology and Biotechnology
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    • v.31 no.4
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    • pp.493-500
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    • 2021
  • Endophytic fungi are symbiotically related to plants and spend most of their life cycle within them. In nature, they have a crucial role in plant micro-ecosystem. They are harnessed for their bioactive compounds to counter human health problems and diseases. Endophytic Diaporthe sp. is a widely distributed fungal genus that has garnered much interest within the scientific community. A substantial number of secondary metabolites have been detected from Diaporthe sp. inhabited in various plants. As such, this minireview highlights the potential of Diaporthe sp. as a rich source of bioactive compounds by emphasizing on their diverse chemical entities and potent biological properties. The bioactive compounds produced are of significant importance to act as new lead compounds for drug discovery and development.

Distribution of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Cirsium japonicum DC. for Wild Vegetables (나물용 엉겅퀴의 근권에서 Arbuscular 균근균의 분포)

  • Cho, Ja-Yong;Heo, Buk-Gu;Yang, Seung-Yul
    • Korean Journal of Organic Agriculture
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    • v.13 no.2
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    • pp.197-209
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    • 2005
  • of Hangalku per plant sold for wild vegetables were 9.1g and 0.9g, and number of leaves was 10.8. Root fresh and dry weights of Hangalku per plant were 19.2g and 4.1g. Thirty five soil samples were collected from the native soils grown Cirsium japonicum DC., and mycorrhizal spores in soils were separated using wet-sieving methods. Number of mycorrhizal spores per 30g fresh soil sized over 500${\mu}$m, 355~500${\mu}$m, 251~354${\mu}$m, 107~250${\mu}$m and 45~106${\mu}$m were 0.6, 2.1, 6.0, 55.3 and 126, etc. Total number of mycorrhizal spores per 30g fresh soil were 190. Root infection by vesicles, hyphae and arbuscules were 13%, 4% and 3%, respectively. As a result of identification, mass propagated mycorrhizal spores by the host plant of Sudangrass were Glomus sp., Gigaspora sp., and Acaulospora sp., and so on.

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Difference of Microorganisms Found in an Aerated submerged Biofilm Reactor with Different Plastic Media Terating Phenol Wastewater (페놀폐수를 처리하는 침적형 생물막 반응기에서 매질에 따른 미생물상의 차이)

  • 정재춘;차병훈
    • Microbiology and Biotechnology Letters
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    • v.23 no.4
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    • pp.492-498
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    • 1995
  • Net type polyvinylidene chloride (PVDC) media and cillium type polyethlene polypropylene (PEPP) media were installed in the aereted submerged biofilm reactors. Synthetic phenol wastewater for feed were made to contain 1,480 mg of phenol per liter of water. The organic loading range of reactors were 0.439-0.456 kg COD/m$_{3}$, 0.882 - 0.919 kg COD/m$_{3}$ and 1.199-1.339 kg COD/m$_{3}$. Comparing PVDC to PEPP media, the bacterial number found in biofilm on PEPP were slightly higher. With the low temperature (10$\circ$C), the number of bacteria was some what deceered. Number of bacterial strains identified from PVDC were 23 and those from PEPP were 42. Genera identified in the PVDC media were Flavobacterium (47.8%), Unidentified (17.6%), Pseudomonas (13.0%), Micrococcus (8.7%) and Beggratoa (8.7%). Genera identified in the PEPP media reactor were Pseudomonas (35.7%), Alcaligenes (19.0%), Aeromonas (14.33%) and Micrococcus (11.9%), In the steady, state, a filamentous bacteria, type 1701 was identified in all of the reactors. Paramecium sp. and fungi were present in the PVDC media reactor. While, Difflugia sp, Paramecium sp. and fungi were found in the PEPP media reactor. The low diversity of protozoa was ascribed to high concentration of phenol.

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