• Title/Summary/Keyword: fungal

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Ectomycorrhizal Fungal Communities of Red Pine (Pinus densiflora) Seedlings in Disturbed Sites and Undisturbed Old Forest Sites

  • Lee, Eun-Hwa;Eom, Ahn-Heum
    • Mycobiology
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    • v.41 no.2
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    • pp.77-81
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    • 2013
  • This study aimed to investigate differences in ectomycorrhizal (ECM) fungal communities between disturbed sites and undisturbed old forest sites. ECM root tips of Pinus densiflora were collected from 4 sites disturbed by human activities and 3 undisturbed old forest sites adjacent to the disturbed sites. Results in this study showed that the number of ECM root tips, species diversity, and number of species were significantly higher in the disturbed sites than in the undisturbed sites, suggesting that the ECM fungal community structure was affected by the degree of disturbance.

Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis

  • Vu, Van-Hanh;Pham, Tuan-Anh;Kim, Keun
    • Mycobiology
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    • v.37 no.4
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    • pp.267-271
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    • 2009
  • A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of $\gamma$-irradiation of $Co^{60}$, ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and $\beta$-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.

Response of the Higher Basidiomycetic Ganoderma resinaceum to Sodium Chloride Stress

  • Mahmoud, Yehia A.-G.;Mohamed, Eman H. F. A.;E. H. F., Abd Elzaher
    • Mycobiology
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    • v.35 no.3
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    • pp.124-128
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    • 2007
  • Ganoderma resinaceum tolerated sodium chloride salt stress within a range of 0 mM till 300 mM. It responded to salt stress with fluctuation in proline formation at different NaCl concentrations. However, the mycelial dry weight, total protein contents and exopolysaccharides did not changed considerably. Increasing sodium chloride concentration led to morphological alteration in fungal mycelia with disappearance of fungal cell wall, plasmolysis, and vacuolation as indicated with electron microscopic examination of the fungal growth.

First Report of Albifimbria verrucaria and Deconica coprophila (Syn: Psylocybe coprophila) from Field Soil in Korea

  • Gurung, Sun Kumar;Adhikari, Mahesh;Kim, Sang Woo;Lee, Hyun Goo;Jun, Ju Han;Gwon, Byeong Heon;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.47 no.3
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    • pp.209-218
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    • 2019
  • During a survey of fungal diversity in Korea, two fungal strains, KNU17-1 and KNU17-199, were isolated from paddy field soil in Yangpyeong and Sancheong, respectively, in Korea. These fungal isolates were analyzed based on their morphological characteristics and the molecular phylogenetic analysis of the internal transcribed spacer (ITS) rDNA sequences. On the basis of their morphology and phylogeny, KNU17-1 and KNU17-199 isolates were identified as Albifimbria verrucaria and Deconica coprophila, respectively. To the best of our knowledge, A. verrucaria and D. coprophila have not yet been reported in Korea. Thus, this is the first report of these species in Korea.

Six New Recorded Species of Macrofungi on Gayasan National Park in Korea

  • Kim, Nam Kyu;Kim, Minkyeong;Lee, Jin Sung;Park, Jae Young;Kim, Changmu
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.385-392
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    • 2021
  • The fungi play important roles in maintaining the balance and homeostasis of natural ecosystems. We investigated fungal specimens indigenous to the Gayasan National Park located in the deep inlands of southeastern Korea from 2017-2020. Six fungal species in the Korean macromycota-Chiua olivaceoreticulata, Entoloma conchatum, Galerina sulciceps, Hebeloma radicosoides, Spongiporus gloeoporus, and Tricholoma sinoacerbum-were identified based on morphological characteristics and rDNA sequences. The six fungal speices were newly revealed on the Korean Peninsula, and it supports that the continuous investigaton is the best way to realize the mycosis.

Four New Records of Ascomycete Species from Korea

  • Nguyen, Thuong T.T.;Pangging, Monmi;Lee, Seo Hee;Lee, Hyang Burm
    • Mycobiology
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    • v.46 no.4
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    • pp.328-340
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    • 2018
  • While evaluating fungal diversity in freshwater, grasshopper feces, and soil collected at Dokdo Island in Korea, four fungal strains designated CNUFC-DDS14-1, CNUFC-GHD05-1, CNUFC-DDS47-1, and CNUFC-NDR5-2 were isolated. Based on combination studies using phylogenies and morphological characteristics, the isolates were confirmed as Ascodesmis sphaerospora, Chaetomella raphigera, Gibellulopsis nigrescens, and Myrmecridium schulzeri, respectively. This is the first records of these four species from Korea.

Systemic Candidiasis of a Wild Slaty-Backed Gull (Larus schistisagus) in Jeju, Korea

  • Yang, Hyoung-Seok;Kim, Jae-Hoon
    • Journal of Veterinary Clinics
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    • v.36 no.3
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    • pp.172-175
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    • 2019
  • A young slaty-backed gull was found dead nearby Seongsan harbor, Seogwipo-si, Jeju. Grossly, focal ulceration and a protuberant nodule were observed in intermediated zone between proventriculus and gizzard. Numerous discrete or confluent white-yellowish nodules were scattered on the surface of liver. Caudal portion of left kidney was replaced by a white-yellowish nodule. Three raised masses were observed in the spleen. Histologically, there were severe ulceration with hemorrhage in the gizzard and multifocal granulomatous inflammations in liver, kidney, spleen, and lungs. Pink to dark-red, round to oval fungal spores and pseudohyphae in granulomatous lesions were confirmed by periodic acid-Schiff stain. Candida albicans were isolated from the lesions of liver, kidney, spleen and gizzard using fungal culture and confirmed by VITEK 2 system. Based on the gross, histopathological findings, and fungal examination, this case was diagnosed as systemic candidiasis of a wild slaty-backed gull. In our best knowledge, this is the first report for systemic candidiasis characterized by multifocal granulomatous lesions in internal organs of a wild gull in Korea.

Identification and Characterization of Unreported Penicillium Species in Korea

  • Choi, Doo-Ho;Kim, Young-Guk;Lee, In-Seon;Hong, Seung-Bum;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.445-456
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    • 2020
  • Fungal species belonging to the genus Penicillium are indigenous to a wide range of natural environments. Since the first published reports detailing the discovery of Penicillium, new species have been identified in various countries, including Korea. We present here a full characterization of five species of Penicillium that were previously unreported in Korea. Based on the morphologic characteristics and sequences of genes encoding fungal ��-tubulin and calmodulin, we identified five Penicillium species, including P. hetheringtonii, P. sublectaticum, and P. jacksonii, which have been unrecorded in Korea, and confirming P. maximae and P. cremeogriseum, as the endophytic fungi isolated in Gyeongsang province, Korea. In this article, we provide detailed morphological descriptions of these fungal species.

Six Newly Recorded Fungal Taxa from Freshwater Niche in Korea

  • Lim, Hyo Jin;Nguyen, Thuong T.T;Lee, Hyang Burm
    • Mycobiology
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    • v.49 no.2
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    • pp.105-121
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    • 2021
  • Six interesting fungal strains were isolated during a survey of fungal diversity associated with freshwater; these strains were designated as CNUFC YJW2-22, CNUFC MSW11-6-2, CNUFC HRS5-3, CNUFC MSW242-6, CNUFC DMW2-2, and CNUFC CPWS-1. Based on a polyphasic approach including phylogenetic analyses of internal transcribed space (ITS), large subunit (LSU), beta-tubulin (BenA), and calmodulin (CaM) gene sequences, morphological analyses, the six strains were found to be identical to Acremonium guillematii, Cadophora novi-eboraci, Lectera nordwiniana, Mycoarthris corallina, Talaromyces siamensis, and Tetracladium globosum, respectively. To our knowledge, these are the first records of the rare Lectera, Mycoarthris, and Tetracladium genera in Korea, and the first reports of A. guillematii, C. novi-eboraci, L. nordwiniana, M. corallina, T. siamensis, and Te. globosum in a freshwater environment.

Unveiling the Functions of the VosA-VelB Target Gene vidD in Aspergillus nidulans

  • Son, Ye-Eun;Park, Hee-Soo
    • Mycobiology
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    • v.49 no.3
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    • pp.258-266
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    • 2021
  • The velvet regulators VosA and VelB are primarily involved in spore maturation and dormancy. Previous studies found that the VosA-VelB hetero-complex coordinates certain target genes that are related to fungal differentiation and conidial maturation in Aspergillus nidulans. Here, we characterized the VosA/VelB-inhibited developmental gene vidD in A. nidulans. Phenotypic analyses demonstrated that the vidD deleted mutant exhibited defect fungal growth, a reduced number of conidia, and delayed formation of sexual fruiting bodies. The deletion of vidD decreased the amount of conidial trehalose, increased the sensitivity against heat stress, and reduced the conidial viability. Moreover, the absence of vidD resulted in increased production of sterigmatocystin. Together, these results show that VidD is required for proper fungal growth, development, and sterigmatocystin production in A. nidulans.