• Journal of Institute of Control, Robotics and Systems
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• v.16 no.8
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• pp.780-785
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• 2010

The OPRoS (Open Platform for Robotic Services) framework is proposed as an application runtime environment for service robot systems. For the successful deployment of the OPRoS framework, fault tolerance support is crucial on top of its basic functionalities of lifecycle, thread and connection management. In the previous work [1] on OPRoS fault tolerance supports, we presented a framework-based fault tolerance architecture. In this paper, we extend the architecture with component-based fault tolerance techniques, which can provide more simplicity and efficiency than the pure framework-based approach. This argument is especially true for fault detection, since most faults and failure can be defined when the system cannot meet the requirement of the application functions. Specifically, the paper applies two widely-used fault detection techniques to the OPRoS framework: 'bridge component' and 'process model' component techniques for fault detection. The application details and performance of the proposed techniques are demonstrated by the same application scenario in [1]. The combination of component-based techniques with the framework-based architecture would improve the reliability of robot systems using the OPRoS framework.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1093-1098
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• 2004

An open reading frame encoding CadC, consisting of 526 amino acids, was identified from the upstream region of the Vibrio vulnificus cadBA operon. The deduced amino acid sequences of the cadC were 22 to 78% similar to those reported from other Enterobacteriaceae. Functions of cadC gene on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutant, whose cadC gene was inactivated by allelic exchanges. The results demonstrated that the gene product of cadC contributes to acid tolerance of V. vulnificus, and that its contribution is dependent on prior exposure of cells to moderately acidic pH. The cellular level of cadB and cadA transcripts decreased in the cadC mutant, indicating that CadC exerts its effect on acid tolerance of V. vulnificus by enhancing the expression of cadBA in a pH-dependent manner.

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.339-343
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• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.

• Convergence Security Journal
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• v.5 no.4
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• pp.41-48
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• 2005

Objective of this study is to design and implement security system based on intrusion tolerance technology for the improvement of dependability in defense system. In order to do so, I identify and extract core technologies through the research and analysis into characteristics, structures, main functions, and technologies of intrusion tolerance architecture. And I accomplish a design of security system through the redundant system based on these core technologies. To implement and verify intrusion tolerance system, I chose 'wargame system' as a subjected system, and accomplished 'Wargame Intrusion Tolerance System' and verified security required functions through a performance test. By applying showed security system into the development of application software based on intrusion tolerance, systematic and efficient system could be developed. Also applying 'WITDS' can solve the current security problems, and this will be basic model for design of security architecture in the federation system after.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.7
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• pp.61-68
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• 1998

The form accuracy of parts has become an important parameter. Therefore, dimensional tolerance and geometric tolerance are used in the design stage to satisfy required quality and functions of parts. But the informations on the machining conditions, which can satisfy the assigned geometric tolerance in design, are insufficient. The objectives of this research are to study the effects of the grinding parameters such as traverse speed, work speed, depth of cut, and dwell time on the after-ground workpiece shape, and to find out the major parameters among them. The results are as follows, The effects of work speed and depth of cut on workpiece shape are negligible compared with the effect of traverse speed. There is an optimal dwell time depending on the traverse speed. The optimal dwell time is decreasing as the traverse speed is increased.

• Asian Journal of Turfgrass Science
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• v.10 no.1
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• pp.31-40
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• 1996

The mechanical expresion provides for the use of Soil property reserves and permanent protec-tion or improvement of soil resources in accordance with measurable standards. If the functions I (initial soil property), E (soil erosion), R (soil renewal), and M. (minimum allowable value) are assumed to be integrable in region A, erosion tolerance over a region is leaded to ${\int}_A{\int}I(m, cl, re, ch, b)dA-{\int}_A{\int}{\{\int}_{to}^{\infty}[E(w, re, c, re, ch, b, t)-R(m, ch, re, b, t)]dt}\dA{\geqq}{\int}_A{\int}M_i(m, cl, re, ch, b)dA$ were variable factors are m=parent material of soil, cl=climate, re=relief or topography, ch=soil characteristics, r=rain or water, w=wind, b=biota, and t=time.

• Molecules and Cells
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• v.38 no.1
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• pp.26-32
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• 2015

Toll-like receptors (TLR) 7 and 9 transduce a cellular signal through the MyD88-dependent pathway and induce the production of inflammatory mediators against microbial nucleotide components. The repeated stimulation of TLR4 leads to endotoxin tolerance, but the molecular mechanisms of tolerance induced through the costimulation of individual TLR has not yet been established, although endosomal TLRs share signaling pathways with TLR4. In the present study, mouse macrophages were simultaneously stimulated with the TLR7 agonist, gardiquimod (GDQ), and the TLR9 agonist, CpG ODN 1826, to examine the mechanism and effector functions of macrophage tolerance. Compared with individual stimulation, the costimulation of both TLRs reduced the secretion of TNF-${\alpha}$ and IL-6 through the delayed activation of the NF-${\kappa}B$ pathway; notably, IL-10 remained unchanged in costimulated macrophages. This tolerance reflected the early induction of suppressor of cytokine signaling-1 (SOCS-1), according to the detection of elevated TNF-${\alpha}$ secretion and restored NF-${\kappa}B$ signaling in response to the siRNA-mediated abrogation of SOCS-1 signaling. In addition, the restimulation of each TLRs using the same ligand significantly reduced the expression of both TLRs in endosomes. These findings revealed that the costimulation of TLR7 and TLR9 induced macrophage tolerance via SOCS-1, and the restimulation of each receptor or both TLR7 and TLR9 downregulated TLR expression through a negative feedback mechanisms that protects the host from excessive inflammatory responses. Moreover, the insufficient and impaired immune response in chronic viral infection might also reflect the repeated and simultaneous stimulation of those endosomal TLRs.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2003.05a
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• pp.208-216
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• 2003

ISO 14649 (data model for STEP-NC) is a new interface scheme or language for CAD-CAM-CNC chain under established by ISO TC184 SCI. Up to this point, the new language is mainly made for milling and turning, and other processes such as EDM will be completed in the future. Upon completion, it will be used as the international standard language for e-manufacturing paradigm by replacing the old machine-level language, so called M&G code used since 1950's. With the rich information contents included in the new language, various intelligent functions can be made by the CNC as the CNC knows what-to-make and how-to-make. In particular, On-Machine Inspection required for quality assurance in the machine level, can be done based on the information of feature­based tolerance graph. Previously, On-Machine inspection has been investigated mainly for milling operation, and only a few researches were made for turning operation without addressing the data model. In this thesis, we present a feature-based on-machine inspection process by the 4 Tasks: 1) proposing a new schema for STEP-NC data model, 2) converting the conventional tolerance scheme into that of STEP-NC, 3) modifying the tolerance graph such that the tolerance can be effectively measured by the touch probe on the machine, and 4) generating collision-free tool path for actual measurement. Task 1 is required for the incorporation of the presented method in the ISO 14649, whose current version does not much include the detailed schema for tolerance. Based on the presented schema, the tolerance represented in the conventional drafting can be changed to that of STEP-NC (Task 2). A special emphasis was given to Task 3 to make the represented tolerance accurately measurable by the touch probe on the machine even if the part setup is changed. Finally, Task 4 is converting the result of Task into the motion of touch probe. The developed schema and algorithms were illustrated by several examples including that of ISO 14649 Part 12.

• BMB Reports
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• v.38 no.6
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• pp.717-724
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• 2005

The nontoxic B subunit of cholera toxin (CTB) can significantly increase the ability of proteins to induce immunological tolerance after oral administration, when it was conjugated to various proteins. Recombinant CTB offers great potential for treatment of autoimmune disease. Here we firstly investigated the feasibility of silkworm baculovirus expression vector system for the cost-effective production of CTB under the control of a strong polyhedrin promoter. Higher expression was achieved via introducing the partial non-coding and coding sequences (ATAAAT and ATGCCGAAT) of polyhedrin to the 5' end of the native CTB gene, with the maximal accumulation being approximately 54.4 mg/L of hemolymph. The silkworm bioreactor produced this protein vaccine as the glycoslated pentameric form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB. Further studies revealed that mixing with silkworm-derived CTB increases the tolerogenic potential of insulin. In the nonconjugated form, an insulin : CTB ratio of 100 : 1 was optimal for the prominent reduction in pancreatic islet inflammation. The data presented here demonstrate that the silkworm bioreactor is an ideal production and delivery system for an oral protein vaccine designed to develop immunological tolerance against autoimmune diabetes and CTB functions as an effective mucosal adjuvant for oral tolerance induction.

• BMB Reports
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• v.38 no.3
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• pp.334-342
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• 2005

Recently, the existence of T cells with dual T cell receptor (TCR) in the immune system is generally accepted, while it has been controversial whether signals through one TCR would affect the functions of the other. In this study T cells expressing two different TCR were obtained from cross-hybrids of LCMV and AND TCR transgenic mice specific for the gp33 and peptide fragment of PCC (fPCC), respectively. Peptide stimulation demonstrated that the dual TCR T cells functioned independently in an antigen-specific manner. To examine whether the tolerance targeted for the one TCR affects the responsiveness of the other, the cross-hybrids were treated with gp33. Although T cells from F1 mice were rendered anergenic to gp33, no functional changes to fPCC were observed in terms of cellular proliferation and IL-2 secretion, suggesting that the dual TCR T cells remained reactive to fPCC. We therefore propose that signaling through the TCR is receptor-specific and 'negative dominance' of one TCR by tolerance induction is not applicable in this dual TCR system.