• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.76-78
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• 2002

Heat shock proteins (HSPs) are induced in most living cells by mild heat treatment, ethanol, heavy metal ions and hypoxia. In yeast Saccharomyces cerevisiae, mild heat pretreatment strongly induces Hsp104 and thus provide acquired thermotolerance. The ability of hsp104 deleted mutant $({\triangle}hsp104)$ to acquire tolerance to extreme temperature is severely impaired. In providing thermotolerance, two ATP binding domains are indispensible, as demonstrated in ClpA and ClpB proteases of E. coli. The mechanisms by which Hsp104 protects cells from severe heat stress are not yet completely elucidated. We have investigated regulation of mitochondrial metabolic pathways controlled by the functional Hsp104 protein using $^{13}C_NMR$ spectroscopy and observed that the turnover rate of TCA cycle was enhanced in the absence of Hsp104. Production of ROS, which are toxic to kill cells radiply via oxidative stress, was also examined by fluorescence assay. Mitochondrial dysfunction was manifested in increased ROS levels and higher sensitivity for oxidative stress in the absence of Hsp104 protein expressed. Finally, we have identified mitochondrial complex I and Ferritin as binding protein(s) of Hsp104 by yeast two hybrid experiment. Based on these observations, we suggest that Hsp104 protein functions as a protector of oxidative stress via either keeping mitochondrial integrity, direct binding to mitochonrial components or regulating metal-catalyzed redox chemistry.

• Ocean Science Journal
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• 제42권2호
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• pp.69-79
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• 2007

A vertical transect with 4 km length was established for the macrofaunal survey on the Chokchon macrotidal flat in Kyeonggi Bay, Incheon, Korea, 1994. Tidal elevation (m) and sediment mean grain size $(\phi)$ were inversely predicted by the transfer functions from the faunal assemblages. Three methods: weighted average using optimum value (WA), tolerance weighted version of the weighted average (WAT) and maximum likelihood calibration (MLC) were employed. Estimates of tidal elevation and mean grain size obtained by using the three different methods showed positively corresponding trends with the observations. The estimates of MLC were found to have the minimum value of sum of squares due to errors (SSE). When applied to the previous data $(1990\sim1992)$, each of three inference models exhibited high predictive power. This result implied there are visible relationships between species composition and faunas' critical environmental factors. Although a potential significance of the two major abiotic factors was re-affirmed, a weak tendency of biological interaction was detected from faunal distribution patterns across the flat. In comparison to the spatial and temporal patterns of the estimates, it was suggested that sediment characteristics were the primary factors regulating the distribution of macrofaunal assemblages, rather than tidal elevation, and the species composition may be sensitively determined by minute changes in substratum properties on a tidal flat.

• IMMUNE NETWORK
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• 제14권6호
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• pp.277-288
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• 2014

T cells are central players in the regulation of adaptive immunity and immune tolerance. In the periphery, T cell differentiation for maturation and effector function is regulated by a number of factors. Various factors such as antigens, co-stimulation signals, and cytokines regulate T cell differentiation into functionally specialized effector and regulatory T cells. Other factors such as nutrients, micronutrients, nuclear hormones and microbial products provide important environmental cues for T cell differentiation. A mounting body of evidence indicates that the microbial metabolites short-chain fatty acids (SCFAs) have profound effects on T cells and directly and indirectly regulate their differentiation. We review the current status of our understanding of SCFA functions in regulation of peripheral T cell activity and discuss their impact on tissue inflammation.

• 전기학회논문지P
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• 제59권3호
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• pp.275-288
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• 2010

In order to improve the quality of electricity in large scale power systems, stability of power system has to be achieved. This can be done by the means of preventative diagnosis of power equipments and protection, monitoring and control of the power system. Since the recent adoption of digital controllers, an improvement in stability was observed; in particular, IED, which contained self-diagnostic abilities such as fault tolerance, allowed for automatic recovery via redundancy or switching-over functions should there be faults with the equipments. Furthermore, communication lines have been hugely simplified, thus adding to the improvement in stability significantly. Taking these error reports and forecasting emergency reports and by effectively responding to them in the overiding controlling systems, high levels of system stability can be obtained. Power Management System that is being applied to automated power sub-stations, takes the IEC61850 international standard as its specification. In this paper, additional research into achieving stability of already developed PMS system and also the stability of the overall system was carried out, and the results of development of communication servers, which play a pivotal role in connecting systems, are stated.

• Genomics & Informatics
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• 제20권2호
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• pp.17.1-17.11
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• 2022

Genetic associations have been quantified using a number of statistical measures. Entropy-based mutual information may be one of the more direct ways of estimating the association, in the sense that it does not depend on the parametrization. For this purpose, both the entropy and conditional entropy of the phenotype distribution should be obtained. Quantitative traits, however, do not usually allow an exact evaluation of entropy. The estimation of entropy needs a probability density function, which can be approximated by kernel density estimation. We have investigated the proper sequence of procedures for combining the kernel density estimation and entropy estimation with a probability density function in order to calculate mutual information. Genotypes and their interactions were constructed to set the conditions for conditional entropy. Extensive simulation data created using three types of generating functions were analyzed using two different kernels as well as two types of multifactor dimensionality reduction and another probability density approximation method called m-spacing. The statistical power in terms of correct detection rates was compared. Using kernels was found to be most useful when the trait distributions were more complex than simple normal or gamma distributions. A full-scale genomic dataset was explored to identify associations using the 2-h oral glucose tolerance test results and γ-glutamyl transpeptidase levels as phenotypes. Clearly distinguishable single-nucleotide polymorphisms (SNPs) and interacting SNP pairs associated with these phenotypes were found and listed with empirical p-values.

• 콘크리트학회논문집
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• 제17권1호
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• pp.157-166
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• 2005

This article involves architecting prototype-fuzzy expert system for designing the nominal cover thickness by means of fuzzy inference for quantitatively representing the environment affecting factor to reinforced concrete in chloride-induced corrosion environment. In this work, nominal cover thickness to reinforcement in concrete was determined by the sum of minimum cover thickness and tolerance to that defined from skill level, constructability and the significance of member. Several variables defining the quality of concrete and environment affecting factor (EAF) including relative humidity, temperature, cyclic wet and dry, and the distance from coast were treated as fuzzy variables. To qualify EAF the environment conditions of cycle degree of wet-dry, relative humidity, distance from coast and temperature were used as input variables. To determine the nominal cover thickness a qualified EAF, concrete grade, and water-cement ratio were used. The membership functions of each fuzzy variable were generated from the engineering knowledge and intuition based on some references as well as some international codes of practice.

• 한국초지조사료학회지
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• 제36권3호
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• pp.243-247
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• 2016

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

• The Plant Pathology Journal
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• 제29권4호
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• pp.471-476
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• 2013

Plants are frequently exposed to numerous environmental stresses such as dehydration and high salinity, and have developed elaborate mechanisms to counteract the deleterious effects of stress. The phytohormone abscisic acid (ABA) plays a critical role as an integrator of plant responses to water-limited condition to activate ABA signal transduction pathway. Although perception of ABA has been suggested to be important, the function of each ABA receptor remains elusive in dehydration condition. Here, we show that ABA receptor, pyrabactin resistance-like protein 8 (PYL8), functions in dehydration conditions. Transgenic plants overexpressing PYL8 exhibited hypersensitive phenotype to ABA in seed germination, seedling growth and establishment. We found that hypersensitivity to ABA of transgenic plants results in high degrees of stomatal closure in response to ABA leading to low transpiration rates and ultimately more vulnerable to drought than the wild-type plants. In addition, high expression of ABA maker genes also contributes to altered drought tolerance phenotype. Overall, this work emphasizes the importance of ABA signaling by ABA receptor in stomata during defense response to drought stress.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제4권6호
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• pp.1273-1293
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• 2010

Immune-inspired intrusion detection is a promising technology for network security, and well known for its diversity, adaptation, self-tolerance, etc. However, scalability and coverage are two major drawbacks of the immune-inspired intrusion detection systems (IIDSes). In this paper, we propose an IIDS framework, named GEP-IIDS, with improved basic system elements to address these two problems. First, an additional bio-inspired technique, gene expression programming (GEP), is introduced in detector (corresponding to detection rules) representation. In addition, inspired by the avidity model of immunology, new avidity/affinity functions taking the priority of attributes into account are given. Based on the above two improved elements, we also propose a novel immune algorithm that is capable of integrating two bio-inspired mechanisms (i.e., negative selection and positive selection) by using a balance factor. Finally, a pruning algorithm is given to reduce redundant detectors that consume footprint and detection time but do not contribute to improving performance. Our experimental results show the feasibility and effectiveness of our solution to handle the scalability and coverage problems of IIDS.

• 한국전자파학회:학술대회논문집
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• 한국전자파학회 2003년도 종합학술발표회 논문집 Vol.13 No.1
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• pp.213-218
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• 2003

Because of the development of digital technology, modern digital instrumentation & control systems are being innovativly developed in industrial plants. Whereas, many analog systems are still being used in nuclear plants, because of the demerits of digital equipment. As known, the demerits of digital equipment are the uncertainty and weaknesses in ambient environments such as smoke & electromagnetic interference In an Integral Reactor, a digital I&C system will be composed of microprocessor, memory and network card. Designers will apply new technique for digital equipment. Thus, it is important for digital I&C systems to operate according to designed functions & performance in the ambient environments during a life cycle. Digital I&C systems should have tolerance in such environments and environment qualification should be concluded To acquire electromagnetic interference qualification of digital equipment, this paper suggests an EMI test requirement. Designers should consider the electromagnetic compatibility and test digital equipment according to each test procedure. This paper involves an EMI test requirement and the results analysis of EUT(Equipment Under Test). Test result analysis will be used as electromagnetic compatibility design guides for Integral Reactor I&C systems.