• Journal of Embryo Transfer
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• v.32 no.1
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• pp.17-24
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• 2017

Ganglioside GD1a is specifically formed by the addition of sialic acid to ganglioside GM1a by ST3 ${\beta}$-galactoside ${\alpha}$-2,3-sialyltransferase 2 (ST3GAL2). Above all, GD1a are known to be related with the functional regulation of several growth factor receptors, including activation and dimerization of epidermal growth factor receptor (EGFR) in tumor cells. The activity of EGF and EGFR is known to be a very important factor for meiotic and cytoplasmic maturation during in vitro maturation (IVM) of mammalian oocytes. However, the role of gangliosides GD1a for EGFR-related signaling pathways in porcine oocyte is not yet clearly understood. Here, we investigated that the effect of ST3GAL2 as synthesizing enzyme GD1a for EGFR activation and phosphorylation during meiotic maturation. To investigate the expression of ST3GAL2 according to the EGF treatment (0, 10 and 50 ng/ml), we observed the patterns of ST3GAL2 genes expression by immunofluorescence staining in denuded oocyte (DO) and cumulus cell-oocyte-complex (COC) during IVM process (22 and 44 h), respectively. Expression levels of ST3GAL2 significantly decreased (p<0.01) in an EGF concentration (10 and 50 ng/ml) dependent manner. And fluorescence expression of ST3GAL2 increased (p<0.01) in the matured COCs for 44 h. Under high EGF concentration (50 ng/ml), ST3GAL2 protein levels was decreased (p<0.01), and their shown opposite expression pattern of phosphorylation-EGFR in COCs of 44 h. Phosphorylation of EGFR significantly increased (p<0.01) in matured COCs treated with GD1a for 44 h. In addition, ST3GAL2 protein levels significantly decreased (p<0.01) in GD1a ($10{\mu}M$) treated COCs without reference to EGF pre-treatment. These results suggest that treatment of exogenous ganglioside GD1a may play an important role such as EGF in EGFR-related activation and phosphorylation in porcine oocyte maturation of in vitro.

• Journal of Korean Society of Transportation
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• v.33 no.6
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• pp.575-583
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• 2015

The Korea Transport Institute (KOTI) builds the origin and destination(O-D) trip data with relatively smaller zone size such as Eup, Myeon, Dong administration unit districts in metropolitan area. Otherwise, O-D trip data was built by bigger size of traffic analysis zone(TAZ) such as Si, Gun, Gu administration unit districts for rural area. In some cases, it is needed to divide a zone into several sub-zones for rural area in order to analyze travel distribution pattern in detail for a certain highway and rail project. The study suggested a method to estimate O-D trips for sub-zones in the larger-size zones in rural area. Two different distribution models, direct demand model and gravity model, were calibrated for sub-zone's intra-zonal O-D trip pattern with metropolitan area O-D data which has smaller zone-size (sub-zone) data categorized by low, middle and high population density. The calibration results were compared between the two models. The gravity model with impedance function of power functional form was selected with better explanation for all groups in the metropolitan area. The adjusted $R^2$ was 0.7426, 0.6456 and 0.7194 for low, middle and high population density group, respectively. The suggested O-D trip estimating method is expected to produce enhanced trip patterns with sub-divided small zones.

• Molecules and Cells
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• v.27 no.4
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• pp.467-473
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• 2009

Our previous study suggested that OsBWMK1, a gene which encodes a member of the rice MAP kinase family, generates transcript variants which show distinct expression patterns in response to environmental stresses. The transcript variants are generated by alternative splicing and by use of alternative promoters. To test whether the two alternative promoters, pOsBWMK1L (promoter for the OsBWMK1L splice variant) and pOsBWMK1S (promoter for the OsBWMK1S splice variant), are biologically functional, we analyzed transgenic plants expressing GUS fusion constructs for each promoter. Both pOsBWMK1L and pOsBWMK1S are biologically active, although the activity of pOsBWMK1S is lower than that of pOsBWMK1L. Histochemical analysis revealed that pOsBWMK1L is constitutively active in most tissues at various developmental stages in rice and Arabidopsis, whereas pOsBWMK1S activity is spatially and temporally restricted. Furthermore, the expression of pOsBWMK1S::GUS was upregulated in response to hydrogen peroxide, a plant defense signaling molecule, in both plant species. These results suggest that the differential expression of OsBWMK1 splice variants is the result of alternative promoter usage and, moreover, that the mechanisms controlling OsBWMK1 gene expression are conserved in both monocot and dicot plants.

• Korean Journal of Family Social Work
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• no.23
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• pp.263-302
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• 2008

The study had four purposes as follows; First, the researcher tried to find the characteristics of conflict. Second, the researcher attempted to find the factors influencing the conflict between mother and the eldest son, Third, the study attempted to find the therapist's intervention techniques, which were caused the change of the conflict between mother and the eldest son. Lastly, the study was to find the changed contents of family relationships. The total family counseling was 13 sessions, which included individual and family counseling. The study used constant comparative analysis by using open coding method to find the factors. Also, the researcher used Miles and Huberman's matrix and graphical network display to show the relationships among factors influencing the conflicts between mother and the eldest son. The study found the boundary problem of family, family's undifferentiated ego, dysfunctional communication patterns, and mother's experience from family of origin as factors influencing the conflict between mother and the eldest son. On the other hand, the study found that the therapist used the formation of therapeutic alliance relationship, guide of communication skills, the reflection of repeated pattern, reframing and a metaphor as intervention methods. The result of study revealed that changed level of family relationship, activating functional communication, changed boundary among family members, differentiated ego of family members, and changed cognition as the changed contents of family relationship.

• Journal of the Korean Institute of Landscape Architecture
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• v.49 no.2
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• pp.101-112
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• 2021

In Korea, efforts have been made continuously to improve the environment of traditional markets concerning the issues of urban regeneration. In particular, many old cities and traditional markets face a lack of parking spaces. As a solution to this, attempts are being made to prepare underground parking spaces by designing urban planning facilities in three-dimensional ways and utilizing the upper part as a more meaningful space. This study is about the master plan to use the upper green area while creating an underground parking lot at 571 Sinmae-dong, Suseong-gu, Daegu. This green area was defined as a space with dual values, 'defensive green space' that needs to be ecologically protected, and 'active cultural space' where walking flows to the market and various events are concentrated. Three specific design strategies to balance these values were presented. First, to prevent indiscriminate occupation and damage by people and maintain a healthy green environment, securing the maximum amount of undivided green space in the site was suggested. Second, a space layout and a topography and planting patterns that can overcome the morphological characteristics of narrow and long-shaped sites enable the experience of abundant green spaces. Third, providing space to strengthen the connections with nearby urban facilities such as Sinmae Market and Gosan Library can also intensively accommodate cultural activities in various cities. This study has academic significance in providing implications for urban regeneration projects with similar contexts in the future.

• (The)Study of the Eastern Classic
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• no.73
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• pp.415-444
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• 2018

This study was designed to analyze the delivery patterns of early(1900-1945) letters from the beginning of the postal service in order to examine the process of establishing the modern postal system after the constitution of the Postal Service(1884). In Chapter 2, we examined the 'change of the way letters are delivered', Chapter 3 is about the 'change of expression' and Chapter 4 is about 'double-faced of the post'. In the examination of aspects of changing the way letters were delivered, the phase of postal delivery by personal delivery to postal delivery were divided into three phases: [the period of personal delivery delivery] > [the coexistence period of personal delivery and postal deliveryy] > [the period of postal delivery]. In particular, it was interesting to see that the same sender and receiver mixed up postal delivery and personal delivery by the case in the second period. In addition, the functional differences between the express, telegram and particular post were found in the examination fo contents of the letter. In the examination of aspects of changing in expression, a change of common expression in 'greeting, ending, and receiving expressions' were confirmed. Also, it was confirmed that 'woo-pyeon' was not used as the basic term, but instead replaced with various terms, and that 'woo' was used independently and productively. In the double-faced aspects of post, there were disadvantages of the postal delivery such as 'discontent of the lost' and 'security of confidentiality', despite the advantages of 'economic, rapid, and long-distance delivery'. Because the disadvantage of postal delivery could be supplemented by personal delivery, postal delivery and personal delivery could coexist for a considerable period of time.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.1
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• pp.39-46
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• 2022

In this study, we tried to find out the value of using Vietnamese Ternstroemia kwangtungensis Merr. (TK) extracts as a functional material. As a result of measuring the level of Nrf2 expression of TK, 50% ethanol extract TK leaf showed the highest activity at a concentration of 200 ㎍/mL. In order to confirm the stability of the extract over time at room temperature, UPLC was performed, and stability was maintained because the patterns of the UV spectrum pattern were the same. In order to confirm the antioxidant efficacy of TK, the activity increased as the concentration increased as a result of measuring electron donating and ABTS⁺ radical scavenging ability. At 1,000 ㎍/mL, they showed effects of 94.6% and 90.8%, respectively. As a result of measuring the tyrosinase inhibitory activity showed an inhibitory ability of 24.4% at a concentration of 1,000 ㎍/mL. In addition, as a result of examining the elastase inhibitory activity of the extract was increased in a concentration-dependent manner, and it was confirmed that the inhibitory activity was 42.7% at 1,000 ㎍/mL. From these results, it was confirmed that the TK extract was valuable as a natural material for cosmetics.

• Journal of the Korean Society for information Management
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• v.40 no.3
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• pp.245-271
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• 2023

The purpose of this study was to assess the perception of e-book subscription services among the digitally native generation in their twenties, who have a high e-book usage rate. This study employed a mixed-methods approach, combining survey responses and usability testing. It aimed to assess the awareness and usage of e-book subscription services among university students in their twenties, a demographic known for their high utilization of electronic devices and e-books. The survey was conducted among 202 university students, and the responses were categorized and examined based on whether they were users or non-users. As a result of the survey, I found there is different awareness of e-book between users and non-users, on the other hand, convenience and portability are the strong point of e-books for users and non-users commonly also. Usability testing was performed on a group of 10 university students in their twenties who had not previously used the 'Millies Library' application, which is renowned as the most widely-used e-book platform. Following the experiment, participants expressed positive feedback regarding various optional features, convenience, design, and cost-effectiveness. However, they also had negative reactions concerning touch errors, malfunctions, functional practicality, a lack of interest, system issues, and the absence of a library.

• Journal of Korea Entertainment Industry Association
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• v.13 no.6
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• pp.25-32
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• 2019

According to a recent KOFICE survey, K-Pop's popularity factor is divided into visual and auditory elements, and the importance of hearing elements is found. Among the various types of singers, idol groups led K-Pop and Black Pink, which topped the list of favorite K-Pop singers in 2018, was selected as the study target. In addition, Twice's music, which is similar to Black Pink's period of activity, has been set as a comparative research scope. The common features in the music of the two groups are the chorus and rhythm structure implemented around melody and Harmony rhythm, and the frequency of use of Major and Minor is greatly different. Through this method and modal interchange, K-Pop from 2016 to 2018 is analyzed. Research shows that first, more than one mode is used as a main melody. When more than one mode is used, different lines in the same line will vary depending on the functional form of the song. Second, the rhythm of the melody expressed with vocals and instruments is repeated based on a two-bar pattern. Finally, when using less chord and slowly expressing the igneous rhythms, there is a way to omit or modify the third notes that determine chord characteristics. Through this study, we can see that the usage patterns of the mode and modal interchange are divided according to the major and minor.

• Journal of Korean Society for Atmospheric Environment
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• v.10 no.E
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• pp.371-386
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• 1994

Ecological examinations of the radial growth Patterns of pine trees(Pinus densiflora Sieb. et Zucc) growing on Mt. Namsan in central Seoul were made to test a Proposition that the pine trees decline due to the influence of air pollution and acid rain, which was proposed by some researchers in Korea, and the potential effects of current level of air pollutants to the growth of the Pine trees in central Seoul have been speculated. Tree-rings of 40 trees sampled at 3 sites of Mt. Namsan were prepared and examined using a Computer-aided Tree-Ring Measuring System at Kookmin University, Korea. Air Pollutant data collected by the Ministry of Environment( MOE ) and the Forestry Research Institute(FRI) were used to infer the general conditions of the environment. Correlation analysis was applied to the data set of tree growth and the other environmental factors. General information derived from the close examination of the tree-rings and the data on air pollution, drought and the other biological conditions suggested that the growth of the pine trees was severely affected by the occurrence of drought(climatic variation), the prevalence of the pine leaf gall midges(insects), and the suppression by the black locust trees(Robinia pseudo-acacia L.) (competition among trees). While the current condition of air pollution in Seoul cannot be categorized as good, the concentrations of air pollutants are not so high as to cause acute damages to the trees. In addition, while the data of rain acidity showed episodic low PHs of under 4.0, the average of them is far less acidic than those which were observed in either northeastern United States or central Europe, where the decline of trees were not solely attributed to any of the air pollutants. Considering the sequential facts that one of the most important environmental factors that affect the growth of trees is weather condition of the forest that the proposition of the decline of the pine trees was made without careful examination of the growth patterns and past growth history of them as well as the complex influences of many other factors including the weather conditions to the growth of trees, and that no objective explanation has been made on the causal relationships between the current condition of air pollution and the growth of the trees, such a proposition should be evaluated as invalid for the explanation of tree growth on Mt. Namsan in central Seoul, Korea. The author evaluates the factors of air pollution (including acid rain) as the predisposing factors, which may have the Potentials to chronically affect the tree growth at the forest ecosystem on Mt. Namsan for a long period of time. Ecosystem ecological studies should be further carried out to carefully explain both the functional and the structural aspects of the ecosystem processes, which include the biogeochemistry and the long-term changes of soil conditions as well as the growth of the other tree species on the mountain.