Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Identification and Characterization of Alternative Promoters of the Rice MAP Kinase Gene OsBWMK1

  • Koo, Sung Cheol (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Choi, Man Soo (National Institute of Crop Science, Rural Development Administration) ;
  • Chun, Hyun Jin (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Park, Hyeong Cheol (Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kang, Chang Ho (Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Shim, Sang In (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Chung, Jong Il (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Cheong, Yong Hwa (Department of Bio-Environmental Science, Sunchon National University) ;
  • Lee, Sang Yeol (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Yun, Dae-Jin (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Chung, Woo Sik (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Cho, Moo Je (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Kim, Min Chul (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
  • Received : 2009.01.19
  • Accepted : 2009.02.26
  • Published : 2009.04.30
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Abstract

Our previous study suggested that OsBWMK1, a gene which encodes a member of the rice MAP kinase family, generates transcript variants which show distinct expression patterns in response to environmental stresses. The transcript variants are generated by alternative splicing and by use of alternative promoters. To test whether the two alternative promoters, pOsBWMK1L (promoter for the OsBWMK1L splice variant) and pOsBWMK1S (promoter for the OsBWMK1S splice variant), are biologically functional, we analyzed transgenic plants expressing GUS fusion constructs for each promoter. Both pOsBWMK1L and pOsBWMK1S are biologically active, although the activity of pOsBWMK1S is lower than that of pOsBWMK1L. Histochemical analysis revealed that pOsBWMK1L is constitutively active in most tissues at various developmental stages in rice and Arabidopsis, whereas pOsBWMK1S activity is spatially and temporally restricted. Furthermore, the expression of pOsBWMK1S::GUS was upregulated in response to hydrogen peroxide, a plant defense signaling molecule, in both plant species. These results suggest that the differential expression of OsBWMK1 splice variants is the result of alternative promoter usage and, moreover, that the mechanisms controlling OsBWMK1 gene expression are conserved in both monocot and dicot plants.

Keywords

Acknowledgement

Supported by : Ministry of Education, Science and Technology of Korea, Korea Science and Engineering Foundation, Rural Development Administration

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