• Bulletin of the Korean Chemical Society
• /
• 제32권8호
• /
• pp.2565-2570
• /
• 2011

The reaction of bromoacetonitrile with 3,14-dimethyl-2,6,13,17-tetraazatetracyclo[$16.4.1^{2.6}.0^{1.18}.0^{7.12}$]tricosane ($L^{10}$) containing a N-$CH_2$-N linkage produces 17-cyanomethyl-3,14-dimethyl-2,6,13,17-tetraazatetracyclo-[$16.4.1^{2.6}.0^{1.18}.0^{7.12}$]tricosane ($L^{11}$). The mono-N-functionalized macrocyclic complexes $[ML^2]^{2+}$ (M = Ni(II) or Cu(II); $L^2$ = 2-cyanomethyl-5,16-dimethyl-2,6,13,17-tetraazatricyclo[$16.4.0.0^{7.12}$]docosane) can be prepared by the reaction of $L^{11}$ with nickel(II) or copper(II) ion in acetonitrile. The N-$CH_2CN$ group attached to $[ML^2]^{2+}$ readily reacts with water or methanol to yield the corresponding complexes of $HL^3$ bearing one N-$CH_2CONH_2$ pendant arm or $L^4$ bearing one $N-CH_2C(=NH)OCH_3$ group. The $N-CH_2CONH_2$ or $N-CH_2C(=NH)OCH_3$ group of each complex is coordinated to the central metal ion. Both $[NiL^4(H_2O)]^{2+}$ and $[CuL^4]^{2+}$ are quite stable in acidic aqueous solutions, but undergo hydrolysis to yield $[Ni(HL^3)(H_2O)]^{2+}$ or $[Cu(HL^3)]^{2+}$ in basic aqueous solutions. In contrast to $[Cu(HL^3)]^{2+}$, $[Ni(HL^3) (H_2O)]^{2+}$ is readily deprotonated to form $[NiL^3 (H_2O)]^+$ ($L^3$ = a deprotonated form of $HL^3$) in basic aqueous solutions.

• 한국응급구조학회지
• /
• 제6권1호
• /
• pp.185-197
• /
• 2002

Today, the basic problem of functional safe management of Korean Disaster Control system was separately administrated 33 acts that are relating to safe management in 13 ministries. Because of the facts that the fire service is not provided practically, the control system and risk management for safe administration are not operated, the information can not be shared with each other, and the various laws have the lacks of linkage, the National Safe System was appeared unsteady. The roles and functions of fire service have started with restriction to operate structurally and institutionally, which operational structure of fire service is becoming weak. As a result, the federal and local fire organizations have not reached yet to the institutionalization and the local fire service agencies have bias with the task regarding the fire service because of the relation between organizational structure and the local fire agency. With the enforcement of the federal and local fire system, professionality and autonomy for making policy, and dealing with changes of fire service positively, the national fire service on the policy performance can be established. Promotion of research and development and education training to strengthen innovation in technology and competition in fire industry will contribute to the firmly establishment of control system to prevent from fire, flood, terror and national disaster. This article proposed that (a) the established law and administration, agency are required efforts to effectively operate fire service system; (b) the national fire service agency, national college of fire, national institute of science fire, and national fire service hospital should be early established to make firmly policy to operate effectively and practically. These kinds of innovational acts are known the best ways of operating solid policy of national fire service system.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.26-26
• /
• 2017

Seed germination stimulated by light is said to be photoblastism. Photoblastism has not been reported in cereal crops, especially in the rice, but Korean weedy rice was reported to have photoblastism and longer mesocotyl than cultivar. Photoblastic weedy rice (PBR) was used to identify QTLs for photoblastism and mesocotyl length. In previous works, QTLs for photoblastism, pbr1 and pbr12 were identified on chromosomes 1 and 12 using 124 F4 lines from a cross between Ilpum and PBR using bulked segregant analysis. Two QTLs for mesocotyl elongation, qMel-1 and qMel-3 were mapped on chromosomes 1 and 3 120 F8 lines from the same cross. Of interest, the RM8260-RM246 region of pbr1 overlapped with a region of qMel-1. To know whether these two QTLs are functionally related, 110 F3 lines were developed from a cross between Ilpum and CR7124. CR7124 having photoblastism and long mesocotyl was selected from 120 F8 lines. 95 F3 lines were measured for germination rate in a light and dark condition and mesocotyl length. Mesocotyl length and germination rate in the dark condition in F3 lines showed significant correlation (r = 0.7, P < 0.0001). 95 $F_3$ lines were genotyped with RM7419 on chromosome 1. ANOVA showed that RM7419 was tightly linked to QTLs for photoblastism as well as mesocotyl length on chromosome 1 (P < 0.0001) indicating the tight linkage of two QTLs. Fine mapping of the two QTL is underway to analyze their functional relationship.

• Asian-Australasian Journal of Animal Sciences
• /
• 제27권10호
• /
• pp.1373-1380
• /
• 2014

The thyroid hormone responsive protein (THRSP) gene is a functional gene that can be used to indicate the fatty acid compositions. This study investigates the relationships of exonic single nucleotide polymorphisms (SNPs) in the THRSP gene and fatty acid composition of muscle fat and marbling score in the 612 Korean cattle. The relationships between fatty acid composition and eight SNPs in the THRSP gene (g.78 G>A, g.173 C>T, g.184 C>T, g.190 C>A, g.194 C>T, g.277 C>G, g.283 T>G and g.290 T>G) were investigated, and according to the results, two SNPs (g.78 G>A and g.184 C>T) in exon 1 were associated with fatty acid composition. The GG and CC genotypes of g.78 G>A and g.184 C>T had higher unsaturated fatty acid (UFA) and monounsaturated fatty acid (MUFA) content (p<0.05). In addition, the $ht1^*ht1$ group (Val/Ala haplotype) in a linkage disequilibrium increased MUFAs and marbling scores for carcass traits (p<0.05). As a result, g.78 G>A and g.184 C>T had significantly relationships with UFAs and MUFAs. Two SNPs in the THRSP gene affected fatty acid composition, suggesting that GG and CC genotypes and the $ht1^*ht1$ group (Val/Ala haplotype) can be markers to genetically improve the quality and flavor of beef.

• BMB Reports
• /
• 제31권1호
• /
• pp.64-69
• /
• 1998

Recent studies have suggested that integrin (CR3) participates in the signal transduction pathways of certain GPI-anchored phagocytic receptors including $Fc{\gamma}RIIIB$. One consequence of this functional linkage is an inducible association between CR3 and cortical microfilaments that is triggered by $Fc{\gamma}RIIIB$ binding to immobilized immune complexes (IC). That this signaling event requires the co-expression of $Fc{\gamma}RIIIB$ with CR3 was documented by the use of NIH 3T3 transfectants expressing both CR3 and $Fc{\gamma}RIIIB$ (clone 3-23), CR3 alone (clone 3-19), and $Fc{\gamma}RIIIB$ alone (clone 3-15). Pretreatment of 3-23 cells with protein kinase inhibitors such as staurosporine and methyl 2,5-dihydroxycinnamate (MDHC) blocked IC-stimulated CR3 microfilament proximity without affecting the extent to which $Fc{\gamma}RIIIB$ constrains the lateral membrane mobility of a subset of CR3 on the cell surface (as measured in fluorescence recovery after photobleaching experiments). These data support that CR3 and $Fc{\gamma}RIIIB$ molecules are physically and functionally associated and that ligation of FcgRIIIB triggers CR3-dependent signal transduction.

• BMB Reports
• /
• 제41권6호
• /
• pp.466-471
• /
• 2008

Three isoforms of pig PDE4B were cloned and classified as two forms: PDE4B1 and PDE4B3, which contain UCR1 and UCR2; and PDE4B2, which contains only UCR2. The amino acid sequences of each isoform showed good conservation in human and rat. PDE4B2 is expressed in a wide range of tissues, but PDE4B1 and PDE4B3 are not. Using an informative SNP for the Iberian x Landrace intercross detected from intron 12, a linkage map was constructed. The location of PDE4B was estimated at 123.6 cM outside of the QTL-CI (124-128 cM) for IMF. However, the QTL-CI for IMF was reconfirmed with high significance, and its position was narrowed down to an interval of 4 cM (the region defined by markers PDE4B and SW1881). Using radiation hybrid mapping, LEPR, LEPROT, DNAJC6, AK3L1 and AK3L2 were selected as positional and/or functional candidates related to the QTL.

• 농촌계획
• /
• 제15권4호
• /
• pp.19-32
• /
• 2009

This research has a significant meaning to break from the urban ecology-oriented biotope research and realize the importance of rural landscape as a reserve area for systemizing the types of rural biotope. The results are as follows. First of all, items for classification of 16 rural biotope areas are designed and total 9 itmes in slope from spatial structural point of view and 7 items in minute-variety from functional point of view. Also, as the result of on-site research on case areas based on classified items, there are 46 types of rural biotope such as coniferous forest, hedgerow and so on. For example, it is proven that uncultivated stripe showed the most frequent emergence. As a result of cluster analysis of average linkage method between clusters, 12 clusters are classified as a clusters and 13 biotope types are re-corrected and complemented through brainstorming process and then total 12 clusters are selected as final rural biotope type groups. As a rural biotope type cluster and character analysis according to types based on on-site research and documentary survey, for example, it is analyzed that the ratio of transmissible covering ratio is 100%, the ratio of green coverage is 90% in 'woodland in cultivated area biotope type cluster'.

• 공학교육연구
• /
• 제18권1호
• /
• pp.47-53
• /
• 2015

이 논문의 목적은 메가 시티 서울의 기술상용화 네트워크 도시 형성의 가능성을 고찰하는 데 있다. 1960년대 이후 우리나라는 국가적 차원에서 볼 때, 급속한 경제 성장을 경험하고 있지만, 지역적 차원에서 살펴보면, 서울을 포함한 수도권과 비수도권 간 심각한 지역불균등 발전을 경험하고 있다. 필자는 이와 같은 지역불균등 발전의 해결 방안으로 서울을 중심으로 기술상용화 네트워크 도시 형성의 필요성을 제시하고자 한다. 기술상용화 네트워크 도시는 남북도시협력 축(통일 R&BD 지향-복합기술 R&BD 축), 메가 시티 확장 축(녹색 수출 R&BD 지향-녹색 수출 R&BD 확장 축), 중소도시 연계 축(평창 올림픽 R&BD 지향-생명기술 R&BD축), 메갈로 폴리스 축(R&D-R&BD 연계 협력 지향-기초기술 R&BD 축) 등 4가지 축으로 이루어진다.

• Journal of Crop Science and Biotechnology
• /
• 제10권4호
• /
• pp.201-210
• /
• 2007

Soybean [Glycine max(L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates(palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid(16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups(LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content(18:1). Six QTLs($R^2$=4-25%) for increased 18:1 in N00-3350(50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid(18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2, and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.

• Reproductive and Developmental Biology
• /
• 제34권2호
• /
• pp.103-109
• /
• 2010

Functional regulation of a specific tissue or organ is controlled by a number of ways, including local cell-cell interaction. Of several forms of cell-cell junctional complexes, gap junctions are caught a great attention due to a formation of direct linkage between neighboring cells. Gap junctions are consisted of connexin (Cx) isoforms. In the present study, we evaluated expressional profiling of Cx isoforms in the rat initial segment (IS) of the male reproductive tract at different postnatal ages. The presence and expression of 13 Cx isoform mRNAs were determined by semi-quantitative real-time PCR analyses. A total of 8 Cx isoform mRNAs were detected in the IS of the male rats during postnatal development. The highest level of Cx30.3 mRNA was found at 5 months of age, while abundance of Cx31 mRNA was the highest at 1 year of age. Expression of Cx31.1 gene was relatively consistent during the postnatal development. Fluctuation of Cx32 and 37 gene expression was observed during the postnatal period. Significant elevation of Cx40 mRNA abundance was detected at 25 days of age and older ages. Expression patterns of Cx43 and 45 genes were similar with the highest level at 2 weeks of age, followed by gradual decreases at older ages. These results indicate differential regulation on expression of Cx isoforms in the rat IS during postnatal development. A complicated regulation of gene expression of Cx isoforms in the IS at different postnatal ages is suggested.