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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Minimizing a QTL region for intramuscular fat content by characterizing the porcine Phosphodiesterase 4B (PDE4B) gene

  • Kim, Jae-Hwan (Division of Applied Life Science, Gyeongsang National University) ;
  • Ovilo, Cristina (Departamento de Mejora Genetica Animal, SGIT-INIA) ;
  • Park, Eung-Woo (Animal Genomics and Bioinformatics, National Institute of Animal Science, RDA) ;
  • Fernndez, Almudena (Departamento de Mejora Genetica Animal, SGIT-INIA) ;
  • Lee, Jun-Heon (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jung-Gyu (Division of Applied Life Science, Gyeongsang National University)
  • Received : 2008.01.30
  • Accepted : 2008.03.06
  • Published : 2008.06.30
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Abstract

Three isoforms of pig PDE4B were cloned and classified as two forms: PDE4B1 and PDE4B3, which contain UCR1 and UCR2; and PDE4B2, which contains only UCR2. The amino acid sequences of each isoform showed good conservation in human and rat. PDE4B2 is expressed in a wide range of tissues, but PDE4B1 and PDE4B3 are not. Using an informative SNP for the Iberian x Landrace intercross detected from intron 12, a linkage map was constructed. The location of PDE4B was estimated at 123.6 cM outside of the QTL-CI (124-128 cM) for IMF. However, the QTL-CI for IMF was reconfirmed with high significance, and its position was narrowed down to an interval of 4 cM (the region defined by markers PDE4B and SW1881). Using radiation hybrid mapping, LEPR, LEPROT, DNAJC6, AK3L1 and AK3L2 were selected as positional and/or functional candidates related to the QTL.

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References

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