• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.77-80
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• 2001

A variety of different methods to generate diverse proteins, including random mutagenesis and recombination, are currently available, and most of them accumulate the mutations on the target gene of a protein, whose sequence space remains unchanged. On the other hand, a pool of diverse genes, which is generated by random insertions, deletions, and exchange of the homologous domains with different lengths in the target gene, would present the protein lineages resulting in new fitness landscapes. Here we report a method to generate a pool of protein variants with different sequence spaces by employing green fluorescent protein (GFP) as a model protein. This process, designated functional salvage screen (FSS), comprises the following procedures： a defective GFP template expressing no fluorescence is firstly constructed by genetically disrupting a predetermined region(s) of the protein, and a library of GFP variants is generated from the defective template by incorporating the randomly fragmented genomic DNA from E. coli into the defined region(s) of the target gene, followed by screening of the functionally salvaged, fluorescence-emitting GFPs. Two approaches, sequence-directed and PCR-coupled methods, were attempted to generate the library of GFP variants with new sequences derived from the genomic segments of E. coli. The functionally salvaged GFPs were selected and analyzed in terms of the sequence space and functional property. The results demonstrate that the functional salvage process not only can be a simple and effective method to create protein lineages with new sequence spaces, but also can be useful in elucidating the involvement of a specific region(s) or domain(s) in the structure and function of protein.

• Fashion & Textile Research Journal
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• v.11 no.4
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• pp.556-565
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• 2009

This study investigates the effect of Alpha-numeric brand name on the product suitability, brand personality, and attitude. To do that we have chosen the functional sportswear, knowing the fact that the Alpha-numeric brand names are not hard to find in this industry. The changes have been made to Alpha-numeric names, such as the usage/non-usage of number, the size of number being used, the writing method, and the different typeface to suit for the study purpose. The results indicate that the Alpha-numeric brand names do have the effect on increasing the fitness for the product's brand. The bigger number and the cursive script will improve the product's suitability. The customers form positive attitude and favorability on the Alpha-numeric brand name compared to the regular brand name in this industry. The contribution of the study is to dig out the distinction of the communicational effects on the customers when the Alpha-numeric brand names take on the different forms. Therefore the current study enlights the practical value of Alpha-numeric brand name as part of the branding strategy.

• Journal of the Korean Society of Costume
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• v.50 no.2
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• pp.15-28
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• 2000

This paper focuses on the sports look expressed in 20th century fashion. It is Age of Sport just as rock'n roll became dominant cultural form of the 1960's and 1970's . In the 1990's the sportsware is fashion. Designers gazing into the future are inspired by the details and functionality of clothing for snow boarding , skiing , motorbike racing and fitness. No doubt fashion in 2000 will also be full of references to it. Since the late 1960's the sportswear that was originally a term for clothing worn for various sports activities in the 1890's has changed and is now considered as fashion for day as well as evening. This has occurred primarily in the U.S. due to the adoption of less formal lifestyles by Americans. while the sportwear is the term which stemmed from the need for functionality in sports, sports look is the style inspired by the formative elements, that is, the details, the shilhouette, and the colors of the sportswear. New technologies for sports, the powerful influence of youthful culture, and the celebritizations of the sports stars made the sports look much hipper and more popular. It can be categoried into three aesthetic values, I. e., the functional sports look,, purism and no useless ornament, the street sports look with fun, androgynous and unisex mode and the mix and match of different texture and colors, and the futuristic sports look with new high tech fabrics and avant-garde style.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.6
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• pp.1020-1027
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• 2003

A traditional mold design has been conducted by an experience-based trial and error, whereby the mold designer would decide the gate locations and processing conditions based on the caring characteristics and its functional requirements. The paper suggests an optimal gate location and processing conditions in the injection molding using a global search method referred to as micro genetic algorithm( ${\mu}$ GA). ${\mu}$ GA yields the optimal solution with a small size of population without respect to design variables for saving time that is needed to calculate the fitness of many individuals. Due to the reason, the paper uses a commercial analysis package of injection molding(CAPA) to analysis a state of flux. In addition to that, axiomatic approach .is applied in the beginning of design. It is a useful method to draw a well-organized and reasonable idea to handle a problem.

• The Research Journal of the Costume Culture
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• v.24 no.6
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• pp.788-802
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• 2016

Sportswear transforms itself into a new fashion item to satisfy desires for beauty in addition to having functionality and movability for sports activities. Sportswear is not in fashion just temporarily but comes in various forms along with sociocultural phenomenon that put emphasis on well-being and leisure. This research aims to study the needs of the bikers who ride bicycles or cycles has become a popular outdoor activity, and give suggestions for high functional sports design that can satisfy the needs of the highly demanding taste of athletes. To understand the design development direction and design preference, practical consumer needs were derived through a survey analysis on bike wear purchasing satisfaction and actual wearing state by bike riders. For consumer needs analysis, focus group interview, in-depth interview and questionnaire method were used. Based on analysis of those researches, athleisure bike wears appropriate for urban sports activities were categorized into sports, life, fitness and leisure 4 styles for athleisure bike wear design development. It is believed that this study will contribute to in the category expansion of sports wear market and satisfy the consumer demands for functional and specialized casual bike wears as well as fashion factors optimized to life style of modern people.

• Korean Journal of Adult Nursing
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• v.28 no.6
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• pp.619-627
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• 2016

Purpose: This study was to test a structural model of spirituality and the quality of life of stroke survivors' caregivers in order to provide guidelines for the development of intervention and strategies to improve their quality of life. Methods: Data were collected from 133 family caregivers of stroke patients who were hospitalized in C university hospital located in Seoul. Data collection using survey questionnaires was done from May, 2013 to February, 2014. Results: Fitness of the hypothetical model was appropriate. Physical component of quality of life of family caregivers is directly affected by two variables (51.5%), burden and depression. Mental component of quality of life of family caregivers is directly affected by three variables (77.6%), depression, burden, and functional dependence of patients. Depression as well as burden were directly affected by spirituality and functional dependence of patients respectively. Thus, spirituality directly affected depression and burden and indirectly affected the quality of life of family caregivers. Conclusion: Therefore, spiritual intervention to improve the stroke caregivers' quality of life might be necessary to support and strengthen their spirituality as a mediating variable that can contribute to decreasing their depression and burden.

• Journal of Korean Academy of Nursing
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• v.40 no.4
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• pp.533-541
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• 2010

Purpose: This study was designed to test structural equation modeling of the quality of life of stroke survivors in order to provide guidelines for development of interventions and strategies to improve their quality of life. Methods: The participants in the study were patients who visited the neurology outpatient department of a tertiary hospital in Seoul between June 25 and October 15, 2009. Data collection was carried out through one-on-one interviews. Demographic factors, functional independence, social support, nutritional status, post-stroke biobehavioral changes and quality of life were investigated. Results: The final analysis included 215 patients. Fitness of the hypothetical model was appropriate (${\chi}^2$=111.5, p=.000, GFI=.926, AGFI=.880, RMSA=.068, NFI=.911, CFI=.953). Functional dependency, social support and post-stroke biobehavioral changes were found to be significant explaining variance in quality of life. Post-stroke biobehavioral changes had the strongest direct influence on quality of life. Nutritional status had an indirect effect on the quality of life. Conclusion: To improve the quality of life of stroke survivors, comprehensive interventions are necessary to manage post-stroke biobehavioral changes, and strengthening social support networks that can contribute to enhancing the quality of life of stroke survivors.

• Journal of Distribution Science
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• v.17 no.2
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• pp.77-89
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• 2019

Purpose - This study presents the relationships between parent brand-self image congruence, parent brand -functional congruity, perceived fit between parent brand and extension product, and consumer's attitude toward extension product using the structural equation model. As for preceding concepts having a direct effect on consumer's attitude toward brand extension, the study focuses on parent brand-self image congruence and parent brand-functional congruity. It also looks at the effect of parent brand-self image congruence and parent brand-functional congruity on the consumer's attitude toward extension product and identifies if the perceived fit between parent brand and extension product is a concept playing a mediating role between parent brand-self image congruence or parent brand-functional congruity and consumer's attitude toward brand extension product. Research design, data, and Methodology - SPSS for Windows 18.0 and AMOS 18.0 were used for data analysis. Then, the Cronbach's α coefficient was obtained using SPSS for Windows 18.0 to measure reliability. In addition, the convergence and discriminative measures of the measured items were statistically verified using AMOS 18.0. Finally, we have validated a path model and a parameter model in which brand personality-self-matched attitude toward brand extension. Results - Studies have shown that the impact of parent brand-self image consistency and parent brand-functional consistency on the attitude of extended products was positive. Perceived fit between parent brand and extension products has been shown to play a role of intermediary between parent brand-self image congruence and consumer attitudes to extended products. However, perceived fit between parent brand and extension products did not play a role as intermediary between the parent brand-functional congruity and the attitude toward the extended product. Finally, in relation to the causal relationship between parent brand-congruity (self image/functionality) and attitudes to extended products. The effect of parent brand-functional coherency on attitudes toward extended products was found to be greater in groups with higher involvement than in those with lower involvement. Conclusions - Although few studies have been done on what variables mediated when parent brand-self image consistency and parent brand-functional coherency affect consumer attitude, this paper is significant in finding whether perceived fitness between the parent brand-congruity and the extension product is mediated.

• Asia pacific journal of information systems
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• v.30 no.1
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• pp.94-119
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• 2020

Digital platforms characterized by network effects enable provisioning of various types of services and provide a mechanism for linking producers and consumers. Identifying the key Quality of Service attributes of such platforms is vital for their continued success and growth. In this paper, a set of quality attributes for platforms is first extracted from different extant quality models. Then actual user feedback data from three platform providers are analysed and mapped against the set of quality attributes to determine the key attributes that are relevant. These findings are corroborated with qualitative data from interviews of different stakeholders. The results show that service quality characteristics are important to the success of platforms. Functional characteristics of platforms assume importance where the digital contributions of the platform is higher. Apart from these, 'fitness for use' as a major determinant of quality is also important in digital platforms.

• Fashion & Textile Research Journal
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• v.22 no.3
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• pp.368-377
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• 2020

This study provided basic data to develop functional and fitness cycling wear for cyclists. We analyzed the domestic and overseas cycling wear brand patterns for tops and tights as well as compared pads attached to tights. The results were as follows. The tops of the cycling wear were designed with cutting lines in the lateral or yoke in the back to reflect cycling posture. It is necessary to study if there is a difference in the air resistance or fitness of the athlete. The sleeve hem band affected by arm movement during cycling, proper sewing and band pressure are also important. The tights have a brand that does not insert a band in front of the waist in consideration of the cyclist's breathing. It should take into account methods of constructing a proper garment pressure that does not interfere with cycling. Analyzing the cycling pad indicated that the three-dimensional shape and size of the pad show many differences for each brand, indicating a difference in cycling movement suitability. In addition, many of the cutting lines should account for the productive aspects because of the high consumption of the fabric in order to design a pattern considering the cycling posture.