• 한국전산구조공학회논문집
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• 제29권5호
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• pp.463-469
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• 2016

본 논문에서는 저차원 확률 함수를 사용하여 연속적인 선형 공극비 기울기를 갖는 기능경사재료 형태의 가상 시편을 제작하였다. 실제 물 시멘트 비가 다른 두 종류 시멘트 풀 시편의 X-선 CT 단면 이미지를 통해 3차원 공극 분포 모델을 제작하여 이를 기반으로 가상 시편을 제작하였다. 가상 시편이 두 실제 시편 사이에 위치하도록 공극 분포 특성을 저차원 확률 함수와 공극비 기울기로 구성하였다. 공극 분포의 기울기는 연속적인 형태의 수직 방향의 선형 공극비 기울기로 구현하며, 이를 위해 확률적 최적화의 목적 함수로 저차원 확률 함수와 공극비 기울기에 관한 함수를 사용하였다. 가상 시편 내 공극 분포 특성 확인을 위해 본 연구에서는 투기율 분석을 유한요소해석을 통해 수행하였다. 공극 분포 특성과 투기율 해석 결과를 비교하여 가상 시편이 갖는 공극 분포 특성이 투기율에 미치는 영향을 확인하고, 투기율의 실제 실험 결과와 해석 결과의 비교를 통해 해석 결과의 타당성을 입증하였다.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.125-132
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• 2010

Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.

• Journal of Plant Biotechnology
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• 제4권1호
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• pp.7-15
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• 2002

Eight cDNAs corresponding to fruit-specific genes were isolated from ripened melon through differential screening. Sequence comparison indicated that six of these cDNAs encoded proteins were previously characterized into aminocyclopropane-1-carboxylate (ACC) oxidase, abscisic acid, stress and ripening inducible (ASR) gene, RINC-H2 zinc finger protein, pyruvate decarboxylase, or polyubiquitin. RFS2 and RFS5 were the same clone encoding polyubiquitin. The other cDNAs showed no significant homology with known protein sequences. The ASR homologue (Asr1) gene was further characterized on the cDNA and genomic structure. The deduced amino acid sequence had similar characteristics to other plant ASR. The Asr1 genomic DNA consisted of 2 exons and 1 intron, which is similar to the structure of other plants ASR genes. The promoter region of the Asr1 gene contained several putative functional cis-elements such as an abscisic acid responsive element (ABRE), an ethylene responsive element (ERE), a C-box or DPBf-1 and 2, Myb binding sites, a low temperature responsive element (LTRE) and a metal responsive element (MRE). The findings imply that these elements may play important roles in the response to plant hormones and environmental stresses in the process of fruit development. The results of this study suggest that the expressions of fruit specific and ripening-related cDNAs are closely associated with the stress response.

• 토지주택연구
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• 제11권2호
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• pp.95-104
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• 2020

This study aims to explore a purely mechanistic pavement analysis approach where viscoelasticity and fracture of asphalt mixtures are considered to accurately predict deformation and damage behavior of flexible pavements. To do so, the viscoelastic and fracture properties of designated pavement materials are obtained through experiments and a fully mechanistic damage analysis is carried out using a finite element method (FEM). While modeling crack development can be done in various ways, this study uses the cohesive zone approach, which is a well-known fracture mechanics approach to efficiently model crack initiation and propagation. Different pavement configurations and traffic loads are considered based on three main functional classes of roads suggested by FHWA i.e., arterial, collector and local. For each road type, three different material combinations for asphalt concrete (AC) and base layers are considered to study damage behavior of pavement. A concept of the approach is presented and a case study where three different material combinations for AC and base layers are considered is exemplified to investigate progressive damage behavior of pavements when mixture properties and layer configurations were altered. Overall, it can be concluded that mechanistic pavement modeling attempted in this study could differentiate the performance of pavement sections due to varying design inputs. The promising results, although limited yet to be considered a fully practical method, infer that a few mixture tests can be integrated with the finite element modeling of the mixture tests and subsequent structural modeling of pavements to better design mixtures and pavements in a purely mechanistic manner.

• International Journal of Automotive Technology
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• 제5권1호
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• pp.33-46
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• 2004

A finite element model development of a 50th percentile male cervical spine is presented in this paper. The model consists of rigid, geometrically accurate vertebrae held together with deformable intervertibral disks, facet joints, and ligaments modeled as a series of nonlinear springs. These deformable structures were rigorously tuned, through failure, to mimic existing experimental data; first as functional unit characterizations at three cervical levels and then as a fully assembled c-spine using the experimental data from Duke University and other data in the NHTSA database. After obtaining satisfactory validation of the performance of the assembled ligamentous cervical spine against available experimental data, 22 cervical muscle pairs, representing the majority of the neck's musculature, were added to the model. Hill's muscle model was utilized to generate muscle forces within the assembled cervical model. The muscle activation level was assumed to be the same for all modeled muscles and the degree of activation was set to correctly predict available human volunteer experimental data from NBDL. The validated model is intended for use as a post processor of dummy measurement within the simulated injury monitor (SIMon) concept being developed by NHTSA where measured kinematics and kinetic data obtained from a dummy during a crash test will serve as the boundary conditions to "drive" the finite element model of the neck. The post-processor will then interrogate the model to determine whether any ligament have exceeded its known failure limit. The model will allow a direct assessment of potential injury, its degree and location thus eliminating the need for global correlates such as Nij.

• 한국실내디자인학회논문집
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• 제27호
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• pp.20-27
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• 2001

The tendency of formalization in Contemporary Architecture deeply relies on the thought of Western Philosophy, which emphasizes the art of image perceived visually but ignores the body and perception of a human beig who uses the architecture and lives in it. On the contrary, Merleau-Ponty asserted long time ago that the world and the body are inseparably related to each other. The phenomenology is important in Architecture, since the center of thought should be taken back to the human body if a artistic meaning can be obtained by Architecture. From this point of view, the meaning of Contemporary Architecture can be renewed by the phenomenological idea of Merleau-Ponty as a means of expanding thought that overcomes the limit of formalization in Contemporary Architecture. This research aims to ream from Steven Holl's work, and show the Architectural elements that are used for preceptual experience of phenomenon and the function of those element sin Architectural space of his works. The result of study on about the phenomenal light and water in Architectural space of Steven Holl is as follows; First, in perceptional experience of phenomenon, time is an important element, which is successive and make a field that cause perceptional experience. Second, light, as a phenomenological element, acted as a means of expressing the comparison and change of light and shadow in Architectural space and showing the change of color by the diversity of time in inner space. Third, water, as a phenomenal lens, not only acted functional but also functioned as an element of sensual experience in Architectural space. It acted as an image containing time, space, just like a mirror that reflect the environment.

• 생명과학회지
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• 제32권11호
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• pp.882-889
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• 2022

Breast cancer anti-estrogen resistance 3 (BCAR3)는 유방암에서 항에스트로겐 내성을 유도하는 유전자들 중의 하나로 발견되었다. 우리는 이미 BCAR3가 c-jun, activator protein-1, serum response element의 promoter 등을 활성화하는 것을 보고하였다. 본 연구에서 우리는 정상 유방세포인 MCF-12A에서 estrogen response element (ERE) 활성에서의 BCAR3의 기능을 조사하였다. BCAR3의 발현이 ERE를 활성화하는 것을 발견하였다. 이 ERE 활성화는 17β-estradiol에 의해 더욱 증가하였고, 이는 항에스트론겐인 tamoxifen에 의해 억제되지 않았다. 다음으로 우리는 ERE 활성화를 이끄는 BCAR3의 신호전달 경로를 연구하였다. BCAR3에 의한 ERE 활성화는 phosphatidylinositol (PI) 3-kinase 경로 억제제인 LY294002와 AZD5363에 의해서는 억제되었으나, Mitogen-activated protein kinase 경로 억제제인 PD98059와 U0126에 의해서는 억제되지 않았다. ERE 활성화는 PI3-kinase의 catalytic subunit p110α와 Akt의 active mutant에 의해서는 유도되었고, 이 활성화는 추가적인 BCAR3에 의해서는 더욱 증가하지 않았다. 이러한 결과로부터 우리는 BCAR3가 PI3-kinase/Akt 신호전달경로를 통하여 ERE 활성화에 중요한 역할을 하는 것을 제시한다.

• 한국산학기술학회논문지
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• 제20권5호
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• pp.599-604
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• 2019

본 연구에서는 스마트폰 사용자의 사용습관 및 하드웨어 특성이 스마트폰의 기능 수행도에 어떻게 영향을 미치는지를 알아보았다. 특히 스마트폰 기능 요소와 사용습관과의 상관관계를 이해하는데 중점을 두었다. 기능성으로는 전부 11 종류의 기능 요소를 정의하였다. 스마트폰 특성은 하드웨어적 특성 및 사용자 사용습관 특성으로 정하였다. 스마트폰 기능별 실측치를 구하기 위해 전부 80명으로 구성된 평가단을 편성하였다. 평가단은 미리 정의된 11개의 기능 요소들에 대해 각 기능 수행에 실제로 소요되는 시간을 스톱워치를 이용하여 측정하였으며 각 기능 요소별로 5회씩 관측하고 데이터를 수집하였다. 기능 요소별 실측치를 종속변수로, 설문지를 통해 수집한 하드웨어 특성, 사용자의 사용습관 등을 전부 12개의 독립변수로 분류하여 Minitab ver.14를 이용하여 회귀분석을 실시하였다. 전반적으로 볼 때, 스마트폰 사용자의 인구통계학적 특성, 하드웨어 특성은 기능 수행에 크게 영향을 미치지 않았다. 그러나 스마트폰 사용습관과 관련한 변수들은 전체적으로 스마트폰 기능 수행도에 커다란 영향을 미쳤으며 그 결과, 과제 수행 시간이 증가하였다. 단순입력 변수 또는 시청 변수에서는 사용성에 미치는 영향은 비교적 미미했지만, 적극 활용 변수에서는 전화걸기, 전화번호 찾기, 사전 검색을 제외한 모든 과제에서 수행시간이 10% - 30%로 크게 증가하였다. 지금까지 스마트폰의 사용자 인터페이스 방식이 크고 단순한 방식으로 일률적으로 제공되었다면, 사용습관이 다양한 계층은 입력 방식이나 과제 처리 방식에 있어서 좀 더 복잡하고 다양한 형태의 인터페이스 방식이 제공되더라도 충분히 활용할 수 있다고 생각한다.

• 한국시뮬레이션학회:학술대회논문집
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• 한국시뮬레이션학회 2001년도 The Seoul International Simulation Conference
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• pp.37-43
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• 2001

New product development has been a key element fur organizational evolution. The bulk of research about new product strategy has focused solely on new product development function itself. This paper investigates cross-functional elements in new product development. More specifically, we suggest that there must exist a fit between new product strategy and value chain strategy. It means that, in order to support new product development activity, there must exist a relevant value chain strategy. We consider three types of integration - internal integration, customer integration, and supplier integration - as strategic elements of value chain strategy. For the case of new product strategy, we consider market newness and product technology unfamiliarity as strategic elements. We also consider two types of learning characteristic, i.e., \\\"fast-adaptive learning\\\" and \\\"slow-adaptive leaning\\\" as control factor. Learning characteristic represents firms organizational capability related with organizational learning. For example, fur fast-adaptive learning case, the effect of integration appears early in time. System dynamics simulation is employed to verify our research framework. The results exhibit that there must exist cross-functional relationships between value chain strategy and new product strategy in order to shorten total development time.al development time.

• Journal of the Optical Society of Korea
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• 제19권1호
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• pp.63-68
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• 2015

A one-step fabrication of a photonic crystal (PC) with functional defects is demonstrated. Using multi-beam phase-controlled holographic lithography with a diffracting optical element, large area one dimensional (1D) and two dimensional (2D) PCs with periodic defects were fabricated. The uniform area is up to $2mm^2$, and tens of defect channels have been introduced in the 1D and 2D PC structure. This technique gives rise to substantial reduction in the fabrication complexity and significant improvement in the spatial accuracy of introducing functional defects in photonic crystals. This method can also be used to design and fabricate three dimensional (3D) PCs with periodic defects.