• Title/Summary/Keyword: functional activity

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Isolation and Selection of Functional Microbes for Eco-friendly Turfgrass Management in Golf Course from Livestock Manure Compost (친환경 잔디관리를 위한 가축분퇴비 중 기능성미생물의 분리 및 선발)

  • Jeong, Je-Yong;Kim, Young-Sun;Cho, Sung-Hyun;Lee, Geung-Joo
    • Weed & Turfgrass Science
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    • v.6 no.2
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    • pp.157-164
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    • 2017
  • Functional microorganisms decompose various organic matter by enzyme activity and suppress plant disease caused by pathogen. This study was conducted to isolate and select functional microorganisms with protein or carbohydrate degradation activities and antagonistic activity against turfgrass fungal pathogens for eco-friendly turfgrass management in golf course from compost containing livestock manure of poultry or swine. Totally 68 isolates collected from livestock manure compost strains were isolated and tested for their activities of amylase, protease and lipase and antagonistic activities against Rhizoctonia solani AG2-2, R. solani AG1-1, and Sclerotinia homoeocarpa. Among the isolates, 34 strains were selected as functional microbes showing higher activities of amylase and protease. Three isolates of ASC-14, ASC-18, and ASC-35 among the 34 strains were selected as antifungal bacterial strains repressing the above 3 turfgrass fungal pathogens. Analysis results of 16s rRNA gene sequence and phylogenic cluster indicated that ASC-14 and ASC-18 belonged to Bacillus amyloliquefaciens, while ASC-35 was B. subtilis, respectively.

Composition Analysis and Antioxidant Activity of Ojuk (Phyllostachys nigra Munro) Leaf Tea and Shoot Tea (오죽잎차와 오죽죽순차의 성분 분석 및 항산화 효과)

  • Kim, Sang-Min;Jeon, Je-Seung;Kang, Suk-Woo;Kim, Woo-Ri;Lee, Ki-Deok;Um, Byung-Hun
    • Journal of Applied Biological Chemistry
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    • v.55 no.2
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    • pp.95-101
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    • 2012
  • Nutritional components of Ojuk leaf tea and Ojuk shoot tea prepared from the leaves and shoots of black bamboo (Phyllostachys nigra Munro) by tea manufacturing process were evaluated. In addition, the extraction yield of water soluble components from these teas in the general tea brewing condition (water extraction in $80^{\circ}C$ for 10 min.) and the contents of polyphenol and flavonoid were compared with not only the dried raw materials, but also green tea and mate tea. Finally, offline and online scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and di(phenyl)-(2,4,6 trinitrophenyl)iminoazanium free radicals were investigated to evaluate the antioxidant activity and explore the components showing ABTS free radical scavenging activity from tea infusion. These results demonstrated that these teas from black bamboo contain various nutritional components and can be used as traditional tea beneficial to human health.

Antibacterial Activity of Essential Oils on the Growth of Staphylococcus aureus and Measurement of their Binding Interaction Using Optical Biosensor

  • Chung, Kyong-Hwan;Yang, Ki-Sook;Kim, Jin;Kim, Jin-Chul;Lee, Ki-Young
    • Journal of Microbiology and Biotechnology
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    • v.17 no.11
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    • pp.1848-1855
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    • 2007
  • Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree>Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were $5.0{\times}10^{-13}\;ml/(CFU{\cdot}s)$ and $5.0{\times}10^5\;ml/CFU$, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.

Structural Requirements of Minoxidil Analogs for Enhancing Lysyl Hydroxylase Inhibitory Activity (Lysyl Hydroxylase의 저해활성을 증가시키기 위한 Minoxidil 유도체들의 구조적인 요건)

  • Myung, Pyung-Keun;Sung, Nack-Do;Lee, Jae-Heung
    • KSBB Journal
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    • v.27 no.2
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    • pp.121-126
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    • 2012
  • In order to explore structural features of minoxidil analogs with a view of enhancing lysyl hydroxylase (LH) inhibitory activity, molecular holographic QSAR (HQSAR) and CoMSIA (comparative molecular similarity indices analysis) were performed. The results from the atomic contributions with optimized the HQSAR 6-2 model indicated that, in case of pyrimidine-1-N-oxide substituent, C2 atom of pyrimidine ring and C'3-C'4 bond of 4-piperidinol group showed the highest impact on the inhibitory activity towards LH enzyme. It was also evident from the information of the optimized CoMSIA F5 model that the inhibitory activity mainly depended on the hydrophobic field contribution (36%) and the hydrogen bond (H-bond) field contribution (49.2%) of substrate molecule. Particularly, it is predicted that the functional groups which disfavor H-bond acceptors in large space around the piperidinol group and also the functional groups which favor the H-bond acceptors at C'4 (& C'5) atom in $R_5$ group play a role for increased inhibitory activity. With this in mind, it is likely that a novel candidate having more improved inhibitory activity on hair growth could be designed in the future.

Comparison of Total Phenol, Flavonoid Contents, and Antioxidant Activities of Orostachys japonicus A. Berger Extracts (와송(Orostachys japonicus A. Berger) 추출물의 총 페놀, 플라보노이드 함량 및 항산화 활성 비교)

  • Jin, Dong-Hyeok;Kim, Han-Soo;Seong, Jong-Hwan;Chung, Hun-Sik
    • Journal of Environmental Science International
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    • v.25 no.5
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    • pp.695-703
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    • 2016
  • The purpose of this study was to measure the bioactivity and antioxidant activity of Orostachys japonicus A. Berger. Orostachys japonicus A. Berger have been known to contain functional materials such as kaempferol, hydroquinone, methyl gallate, quercetin, gallic acid etc. To identify the main functional materials of Orostachys japonicus A. Berger, the contents of flavonoid and phenol were measured. We extracted Orostachys japonicus A. Berger powder from four solvents such as chloroform:methanol (CM, 2:1, v/v), distilled water (DW), 70% methanol, 70% ethanol. After that, this study determined tannin, total phenol, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric reducing antioxidant power and reducing power of Orostachys japonicus A. Berger extracts and as results of comparing each extract. respectively. From the above results shows that antioxidant activity and bioactivity of Orostachys japonicus A. Berger extracts was higher in the order of 70% ethanol, 70% methanol, DW and CM (p<0.05). The results showed that antioxidant activity of Orostachys japonicus A. Berger extracts supposed to affect by the total phenol and flavonoid contents.

A study on the increase of Physiological Activity as a Functional Cosmetic Composition of Hwangryunhaedoktang-Gamibang Fermented with Lactiplantibacillus Plantarum (Lactiplantibacillus plantarum으로 발효한 황련해독탕 가미방의 기능성 화장품 조성물로서의 생리활성 증대에 관한 연구)

  • Seo, Sang Wan
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.35 no.6
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    • pp.228-234
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    • 2021
  • Hwangryunhaedoktang is one of the prescriptions used in traditional medicine for skin diseases. In this study, Hwangryunhaedoktang-Gamibang (HG) was fermented with Lactiplantibacillus plantarum, a probiotic lactic acid bacterium, to evaluate its potential as a functional cosmetic composition. Strains with anti-inflammatory activity were selected by isolating lactic acid bacteria from kimchi, a traditional Korean fermented food. HG was inoculated with lactic acid bacteria and the viability was measured. The supernatant was obtained by centrifugation of fermented Hwangryunhaedoktang-Gamibang (HGF) and HG, and the filtered supernatant was freeze-dried and used in the experiment. By measuring DPPH and ABTS scavenging activity, it was confirmed that the antioxidant activity was increased. RAW264.7 cells were inoculated with HG and HGF to confirm anti-inflammatory activity through NO assay, and production levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured through ELISA assay. It was confirmed that HGF had a greater decrease in production than HG. Through lactobacilli fermentation, the beneficial probiotic properties and antioxidant and pro-inflammatory activities of lactic acid bacteria suggest potential clinical or technical applications.

Structural Alterations of Wogonin Significantly Reduce the Inhibitory Activity against COX-2 Catalyzed PGE2 Production from LPS-Induced RAW 264.7 Cells

  • Gurung, Santosh Kumar;Lim, Hyun;Kim, Hyun-Pyo;Park, Hae-Il
    • Biomolecules & Therapeutics
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    • v.17 no.4
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    • pp.418-421
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    • 2009
  • Structural alterations of wogonin were conducted to observe the role of each functional group at the A-ring of wogonin on the inhibitory activities against COX-2 catalyzed $PGE_2$ production from LPS-induced RAW 264.7 cells. Serial deletion of the functional groups at the A-ring of wogonin exhibited low to comparable bioactivity. The present study validated that all the functional groups at the A-ring of wogonin are important factors to exhibit the optimum inhibitory activities.

Dynamics Study with DFT(Density Functional Theory) Calculation for Metal with a few Peripheral Electrons (범밀도함수론을 이용한 백금, 팔라듐, 니켈, 크롬과 수소반응성 연구)

  • Kim, Taewan;Park, Taesung;Jung, Yeonsung;Kang, Youngjin;Lee, Taeckhong
    • Transactions of the Korean hydrogen and new energy society
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    • v.25 no.3
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    • pp.234-239
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    • 2014
  • To study catalytic activity and hydrogen embrittlement of Pd, Pt, Ni, and Cr in fuel cell electrode, we used density-functional theory. The calculation tools based electron density give much shorter calculation time and cheap costs. Maximum of bond overlap populations of each metal are 0.6539eV for Pd-H, 0.6711eV for Pt-H, 0.6323eV for Ni-H, 0.6152eV for Cr-H. Electron density of Cr has strongest in related metals, which shows strong localization of electron, implying anti hydrogen embrittlement behaviors.

The Actual Conditions of Consumers' Usage of Functional Golfwear (기능성 골프웨어의 소비자 이용실태)

  • Oh, Ry-Gong;Koo, Young-Seok
    • Fashion & Textile Research Journal
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    • v.12 no.2
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    • pp.179-187
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    • 2010
  • The purpose of this study was to investigate the actual conditions of consumers' usage of functional golfwear which is widely used in the current sportswear market. The study was carried out on 225 golfers in Busan and KyungNam area based on the demographics such as gender, age, occupation, and income. The collected data was processed with SPSS 14.0 program using frequency analysis, reliability analysis, t-test, and ANOVA. In the results of the analysis, golfers did not show high reliability and satisfaction on the functional golfwear. In the results of the most function-required golfwear items, there were shirts, pants, footwear, gloves, windbreaker, innerwear etc. in order. Most of golfers answered that the function of golfwear was required for pleasant and comfort golf activity. Therefore, it is necessary for golfwear makers to develop consumer-oriented golfwear products which are reliable and satisfiable to golf consumers.

Identification of a Universal Relation between a Thermodynamic Variable and Catalytic Activities of Pyrites toward Hydrogen Evolution Reaction: Density Functional Theory Calculations (수소발생반응에 대한 Pyrites 표면 촉매 성능 예측: 밀도 범함수 이론 계산)

  • Gang, Jun-Hui;Hwang, Ji-Min;Han, Byeong-Chan
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2017.05a
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    • pp.87.1-87.1
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    • 2017
  • High functional catalyst to efficiently produce clean and earth-abundant renewable fuels plays a key role in securing energy sustainability and environmental protection of our society. Hydrogen has been considered as one of the most promising energy carrier as represented by focused research works on developing catalysts for the hydrogen evolution reaction (HER) from the water hydrolysis over the last several decades. So far, however, the major catalysts are expensive transition metals. Here using first principles density functional theory (DFT) calculations we screen various pyrites for HER by identifying fundamental descriptor governing the catalytic activity. We enable to capture a strong linearity between experimentally measured exchange current density in HER and calculated adsorption energy of hydrogen atom in the pyrites. The correlation implies that there is an underlying design principle tuning the catalytic activity of HER.

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