Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Comparison of Total Phenol, Flavonoid Contents, and Antioxidant Activities of Orostachys japonicus A. Berger Extracts

와송(Orostachys japonicus A. Berger) 추출물의 총 페놀, 플라보노이드 함량 및 항산화 활성 비교

  • Jin, Dong-Hyeok (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University) ;
  • Seong, Jong-Hwan (Department of Food Science and Technology, Pusan National University) ;
  • Chung, Hun-Sik (Department of Food Science and Technology, Pusan National University)
  • Received : 2016.03.04
  • Accepted : 2016.03.23
  • Published : 2016.05.31
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Abstract

The purpose of this study was to measure the bioactivity and antioxidant activity of Orostachys japonicus A. Berger. Orostachys japonicus A. Berger have been known to contain functional materials such as kaempferol, hydroquinone, methyl gallate, quercetin, gallic acid etc. To identify the main functional materials of Orostachys japonicus A. Berger, the contents of flavonoid and phenol were measured. We extracted Orostachys japonicus A. Berger powder from four solvents such as chloroform:methanol (CM, 2:1, v/v), distilled water (DW), 70% methanol, 70% ethanol. After that, this study determined tannin, total phenol, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric reducing antioxidant power and reducing power of Orostachys japonicus A. Berger extracts and as results of comparing each extract. respectively. From the above results shows that antioxidant activity and bioactivity of Orostachys japonicus A. Berger extracts was higher in the order of 70% ethanol, 70% methanol, DW and CM (p<0.05). The results showed that antioxidant activity of Orostachys japonicus A. Berger extracts supposed to affect by the total phenol and flavonoid contents.

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References

  1. Ahn, H. Y., Choe, D. J., Cho, Y. S., 2015, Antioxidant activity and chemical characteristics of Orostachys malacophyllus and fermented Orostachys malacophyllus, J. Life Sci., 25, 577-584. https://doi.org/10.5352/JLS.2015.25.5.577
  2. Arabshahi-Delouee, S., Urooj, A., 2007, Antioxidat properties of various solvent extracts of mulberry (Morus indica L.) leaves, Food Chem., 102, 1233-1240. https://doi.org/10.1016/j.foodchem.2006.07.013
  3. Blois, M. S., 1958, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  4. Bokov, A., Chaudhuri, A., Richardson, A., 2004, The Role of oxidative damage and stress in aging, Mech. Ageing Dev., 125, 811-826. https://doi.org/10.1016/j.mad.2004.07.009
  5. Bravo, L., 1998, Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance, Nutr, Rev., 56, 317-333.
  6. Carlsen, M. H., Halvorsen, B. L., Holte, K., Bohn, S. K., Dragland, S., Sampson, L., Willey, C., Senoo, H., Umezono, Y., Sanada, C., Barikmo, I., Berhe, N., Willett, W. C., Phillips, K. M., Jacobs, D. R., Blomhoff, R., 2010, The Total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide, Nutrition Journal, 9, 1. https://doi.org/10.1186/1475-2891-9-1
  7. Choi, S. Y., Kim, J. G., Jung, N. J., 2008, Studies on the physicochemical characteristics and NDMA formation of Orostachys japonicus A. Berger, J. Korean Soc. Food Sci. Nutr., 21, 148-156.
  8. Droge, W. 2002, Free radicals in the physiological control of cell function, Physiol. Rev., 82, 47-95. https://doi.org/10.1152/physrev.00018.2001
  9. Duan, Y., Kim, M. A., Kim, H. S., Seong, J. H., Lee, Y. G., Kim, D. S., Chung, H. S., 2014, Effects of feral haw (Crataegus pinnatifida Bunge) seed extracts on the antioxidant activities, J. Life Sci., 24, 386-392. https://doi.org/10.5352/JLS.2014.24.4.386
  10. Floegel, A., Kim, D. O., Chung, S. J., Koo, S. I., Chun, O. K., 2011, Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidantrich US foods, J. Food Compos. Anal., 24, 1043-1048. https://doi.org/10.1016/j.jfca.2011.01.008
  11. Graham, H. D., 1992, Stabilization of the Prussian blue color in the determination of polyphenols, J. Agric. Food Chem., 40, 801-805. https://doi.org/10.1021/jf00017a018
  12. Halliwell, B., Gutteridge, J. M., Cross, C. E., 1992, Free radicals, antioxidants, and human disease: Where are we now?, J. Lab. Clin. Med., 119, 598-620.
  13. Hwang, K. A., Kim, G. R., Hwang, Y. J., Hwang, I. G., Song, J., 2016, Oxidative stress inhibitory effects of low temperature-aged garlic (Allium stivum L.) extracts through free radical scavenging activity, J. Korean Soc. Food Sci. Nutr., 45, 27-34. https://doi.org/10.3746/jkfn.2016.45.1.027
  14. Jang, M., Kim, Y. J., Min, J. W., Yang, D. C., 2009, Optimization of extraction method for the quantitative analysis of gallic acid from Cornus officinallis, Korean J. Food Sci. Technol., 41, 498-502.
  15. Jiang, W. Y., 2005, Therapeutic wisdom in traditional Chinese medicine: A Perspective from modern science, Trends Pharmacol. Sci., 26, 558-563. https://doi.org/10.1016/j.tips.2005.09.006
  16. Jing, E. L., Song, T. F., Zeng, H. Q., Chang, L., Shao, P. N., 2015, Total flavonoids content, antioxidant and antimicrobial activities of extracts from Mosla chinensis Maxim. cv. Jiangxiangru, LWT-Food Sci. Technol., 64, 1022-1027. https://doi.org/10.1016/j.lwt.2015.07.033
  17. Kim, E. J., Choi, J. Y., Yu, M., Kim, M. Y., Lee, S., Lee, B. H., 2012, Total polyphenols, Total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants, Korean J. Food Sci. Technol., 44, 337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  18. Kim, E. J., Kim, Y. H., Kim, J. W., Lee, H. H., Ko, Y. J., Park, M. H., Lee, J. O., Kim, Y. S., Ha, Y. L., Ryu, C. H., 2007, Optimization of fermentation process and quality properties of wild grape wine, J. Korean Soc. Food Sci. Nutr., 36, 366-370. https://doi.org/10.3746/jkfn.2007.36.3.366
  19. Kim, H. J., Lee, J. Y., Kim, S. M., Park, D. A., Jin, C., Hong, S. P., Lee, Y. S., 2009, A New epicatechin gallate and calpain inhibitory activity from Orostachys japonicus, Fitoterapia, 80, 73-76. https://doi.org/10.1016/j.fitote.2008.10.003
  20. Kim, H. S., Duan, Y., Kim, M. A., Jang, S. H., 2014, Contents of antioxidative components from pulpy and seed in wild haw (Crataegus pinnatifida BUNGE), J. Environ. Sci. Int., 23, 1791-1799. https://doi.org/10.5322/JESI.2014.23.11.1791
  21. Kim, J. G., Kim, H. L., Kim, S. J., Park, K. S., 2013, Fruit quality, anthocyanin and total phenolic contents, and antioxidant activities of 45 blueberry cultivars grown in Suwon, Korea, J. Zhejiang University-Sci. B (Biomedicine & Biotechnology), 14, 793-799. https://doi.org/10.1631/jzus.B1300012
  22. Lee, J. H., Lee, S. J., Park, S., Kim, H. K., Jeong, W. Y., Choi, J. Y., Sung, N. J., Lee, W. S., Lim, C. S., Kim, G. S., Shin, S. C., 2011, Characterisation of flavonoids in Orostachys japonicus A. Berger using HPLC-MS/MS: Contribution to the overall antioxidant effect, Food Chem., 124, 1627-1633. https://doi.org/10.1016/j.foodchem.2010.08.031
  23. Lee, M. K., Park, Y. S., 2007, Changes of taste quality and catechins content in tea extracts according to elapsed time an ambient temperature, Hort. Environ. Biotechnol., 48, 298-302.
  24. Lee, S. J., Shin, J. H., Ju, J. C., Kang, S. K., Sung, N. J., 2013, Hypoglycemic and hypolipidemic effects of Orostachys japonicus with medicinal herbs in streptozotocin-induced diabetic rats, J. Korean Soc. Food Sci. Nutr., 42, 587-594. https://doi.org/10.3746/jkfn.2013.42.4.587
  25. Lee, S. J., Shin, J. H., Kang, J. R., Hwang, C. R., Sung, N. J., 2012, In vitro evaluation of biological activities of Wa-song (Orostachys japonicus A. Berger) and Korean traditional plants mixture, J. Korean Soc. Food Sci. Nutr., 41, 295-301. https://doi.org/10.3746/jkfn.2012.41.3.295
  26. Lee, S. J., Song, E. J., Lee, S. Y., Kim, K. B. W. R., Kim, S. J., Yoon, S. Y., Lee, C. J., Ahn, D. H., 2009, Antioxidant activity of leaf, stem and root extracts from Orostachys japonicus and their heat and pH stabilities, J. Korean Soc. Food Sci. Nutr., 38, 1571-1579. https://doi.org/10.3746/jkfn.2009.38.11.1571
  27. Lou, H., Hu, Y., Zhang, L., Sun, P., Lu, H., 2012, Nondestructive evaluation of the changes of total flavonoid, total phenols, ABTS and DPPH radical scavenging activities, and sugars during mulberry (Morus alba L.) fruits development by chlorophyll fluorescence and RGB intensity values, Food Sci. Technol., 47, 19-24.
  28. Luthria, D. L., Lu, Y., Maria John, K. M., 2015, Bioactive phytochemicals in wheat: Extraction, analysis, processing, and functional properties, J. Func. Food, 18, 910-925. https://doi.org/10.1016/j.jff.2015.01.001
  29. Ortuno, J., Serrano, R., Jordan, M. J., Banon, S., 2016, Relationship between antioxidant status and oxidative stability in lamb meat reinforced with dietary rosemary diterpenes, Food Chem., 190, 1056-1063. https://doi.org/10.1016/j.foodchem.2015.06.060
  30. Raudonis, R., Raudone, L., Jakstas, V., Janulis, V., 2012, Comparative evaluation of post-column free radical scavenging and ferric reducing antioxidant power assays for screening of antioxidants in strawberries, J. Chromatogr. A, 1233, 8-15. https://doi.org/10.1016/j.chroma.2012.02.019
  31. Rice-Evans C. A., Miller, N. J., Paganga, G., 1997, Antioxidant properties of phenolic compounds, Trends Plant Sci., 2, 152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  32. Schofield, P., Mbugua, D. M., Pell, A. N., 2001, Analysis of condensed tannins: A Review, Anim. Feed Sci. Technol., 91, 21-40. https://doi.org/10.1016/S0377-8401(01)00228-0
  33. Seo, D. J., Jeon, S. B., Oh, H., Lee, B. H., Lee, S. Y., Oh, S. H., Jung, J. Y., Choi, C., 2016, Comparison of the antiviral activity of flavonoids against murine norovirus and feline calicivirus, Food Control, 60, 25-30. https://doi.org/10.1016/j.foodcont.2015.07.023
  34. Singhal, M., Paul, A., Singh, H. P., 2014, Synthesis and reducing power assay of methyl semicarbazone derivatives, J. Saudi Chem. Soc., 18, 121-127. https://doi.org/10.1016/j.jscs.2011.06.004
  35. Wang, M. F., Shao, Y., Li, J. G., Zhu, N. Q., Ho, C. T., 1998, Antioxidantive phenolic compounds from sage (Salvia officinalis), J. Agric. Food Chem., 46, 4869-4873. https://doi.org/10.1021/jf980614b
  36. Won, Y. S., Lee, J. H., Kwon, S. J., Ahn, D. U., Shin, D. Y., Seo, K. I., 2014, Anticancer effects of cultivated Orostachys japonicus on human prostate cancer cells, J. Korean Soc. Food Sci. Nutr., 43, 67-73. https://doi.org/10.3746/jkfn.2014.43.1.067
  37. Wootton, P. C., Moran, A., Ryan, L., 2011, Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin-Ciocalteu methods, Food Res. Int., 44, 217-224. https://doi.org/10.1016/j.foodres.2010.10.033

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