• Korean Journal of Environmental Agriculture
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• v.36 no.3
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• pp.201-210
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• 2017

BACKGROUND: The current study was to monitor of 9 veterinary antibiotics (ceftiofur, clopidol, florfenicol, sulfamethazine, sulfamethoxazole, sulfathiazole, tetracycline, tiamulin, and tylosin) in manure using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive and negative electrospray ionization mode. METHODS AND RESULTS: Sample preparation was carried out using Mcllvaine buffer and citrate salts to adjust the pH of the sample followed by purification with dispersive solid phase extraction (d-SPE). Separation of analytes during LC-MS/MS analysis was conducted using an Eclipse Plus $C_{18}$ column and the mobile phase was in gradient mode with, 0.1% formic acid and 5 mM ammonium formate in methanol (A) and 0.1% formic acid and 5 mM ammonium formate in distilled water (B). The linearity of the matrix-matched calibrations of all tested antibiotics was good, with $R^2$ determination coefficients ${\geq}0.9920$. The limit of detection (LOD) and quantifications (LOQ) were $0.1-67.0{\mu}g/kg$ and $0.4-200.0{\mu}g/kg$, respectively. Analysis of 13 solid and liquid manure samples taken from the Republic of Korea revealed concentrations less than $0.7{\mu}g/kg$ for tiamulin, $1497.6{\mu}g/kg$ for sulfamethazine. CONCLUSION: To monitor 9 veterinary antibiotics from manure samples in 13 provincial areas throughout the Republic of Korea, an analytical method was developed. The developed method was fully validated and successfully applied for monitoring various veterinary antibiotics in manure samples.

• Journal of agriculture & life science
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• v.52 no.6
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• pp.81-88
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• 2018

This study analyzed the characteristics of 6 recyclable bulking agents which can replace sawdust and rice hulls that are commonly used in livestock manure composting. The content of all the hazardous chemicals found in the bulking agents used in this experiment was between 0.0 and 34.1 ppm, which was noticeably lesser than the maximum content levels of hazardous substances 5.0~900.0 ppm prescribed by the Rural Development Administration(RDA), which means that the bulking agents would be able to replace sawdusts and rice husks. The water content of the bulking agents ranged from a minimum of 12.4% to a maximum of 16.6% in the original state, which was much less than 60%, the optimal moisture content. These results indicate that they would be able to function fully as bulking agents. Their water absorption rate was in the range of minimum 31.9% ~ maximum 600.0%, which showed huge differences among the types of bulking agents. The most appropriate bulking agent in terms of water absorption rate was wood pellets. It was the highest in the wood briquette manufactured by A, which was followed by C and B in the order. The shear strength of the 5 types of bulking agents in the original state was 271.7 N on an average, the highest figure of all, except for the oak briquette whose shear strength could not be measured and the wood pellets in the absorption state. The shear strength in the dry and absorption states then followed, and were 78.0 N and 27.7 N on an average, respectively. The wood briquette of A recorded considerably lower shear strength than that of B and C. Overall, shear strength tended to increase according to lower water absorption rates. Since pine wood chips and oak cuts have relatively greater shear strength even in the absorbing state than the other materials, they will be able to endure some power in the case of stirring with pig droppings.