• Archives of Plastic Surgery
• /
• 제37권4호
• /
• pp.323-328
• /
• 2010

Purpose: Adipose-derived stromal cells (ADSCs) are multipotent cells that have been found to promote wound healing through the process of angiogenesis and reepithelialization. Generally, it is well known that the antigenicity of ADSCs doesn't affect stem cell therapy. In this study, we investigated the effect of allogeneic ADSCs in the wound healing process by applying allogeneic ADSCs on the wound healing splint model of mice. Methods: Adipose tissue was harvested from the epididymal fat pads of BALB/c and C57BL/6 mice. Twenty four mice BALB/c were divided into three groups; control, isogeneic, and allogeneic groups. Two full thickness defects with 6 mm diameters were created on the back of BALB/c mice. $1{\times}10^6$ ADSCs from BALB/c mice were applied on the isogeneic group. In the allogeneic group, ADSCs from the C57BL/6 mice were applied. No cells were applied to the control group. The sizes of the wounds were evaluated in 3, 5, 7, 10, and 14 days after the wounds were applied, and tissues were harvested in 7 and 14 days for histological analysis. Results: Wound healing rates had showed significant increase in 10, and 14 days when the isogeneic group was compared to the control group, but the allogeneic group showed significantly decrease compared to the isogeneic group (p<0.05). Histological scores in the isogeneic group were significantly high, but significantly lower in the allogeneic group when compared to the isogeneic group in 2 weeks (p<0.05). In the isogeneic group, thick inflammatory cell infiltration with abundant capillaries were observed in 1 week, and thick epithelium with many large capillaries were observed in 2 weeks. Conclusion: When isogeneic ADSCs were applied to wounds, they presented a faster wound healing rate compared to controls and the allogeneic group. Unlike general stem cell therapy, these findings suggest that cell therapy targeted at enhancing wound healing may benefit from the use of ADSCs with identical antigenicity, as opposed to allogeneic or xenogenic ADSCs.

• Journal of Trauma and Injury
• /
• 제20권1호
• /
• pp.52-56
• /
• 2007

Marjolin's ulcer is a rare and often-aggressive cutaneous malignancy that arises in previously traumatized or chronically inflamed skin, particularly after burns. We experienced two cases after burns. Case I involved a forty eight year-old man who had suffered from a flame burn at the parietal scalp area, where had been initially described three years earlier as a full-thickness wound including the pericranium. The man consulted us for a persistent ulcerative and infected wound on the burned lesion during the last 24 months, which turned out on the contrast-enhanced computed tomography (CT) and magnetic resonance imaging (MRI) to be the squamous cell carcinoma with involving the skull and the dura mater. Although the posterior auricular lymph node was enlarged on the ipsilateral side, recent positron emission tomography (PET) CT did not show any metastatic lesion. It was impossible for us to resect the intracranial involvement of the tumor radically, and the postoperative PET CT still showed a focal fluorodeoxyglucose (FDG) uptake around the wall of the superior sagittal sinus. We think that an aggressive combined approach is essential for treatment in early stages for a high success rate, before the intracranial structures are involved because there is no consensus on the treatment for advanced disease, and the results are generally poor. Case 1 also did not involve a radical resection because of the intracranial invasion to the wall of superior sagittal sinus and the possibility of damage to the major cortical veins. He received adjuvant radiotherapy and must be followed periodically. Case 2 involved an eighty six year-old women who suffered from a painful scalp ulcer lesion after flame burns three years earlier. Unlike case 1, neither tumor infiltration into the dura nor lymph node enlargement was observed on the contrast-enhanced computed tomography (CT), magnetic resonance imaging (MRI), or positron emission tomography (PET) CT. We did a radical resection of the tumor, including the involved bone, and a cranioplasty with bone cement.

• 한국축산시설환경학회지
• /
• 제5권3호
• /
• pp.157-170
• /
• 1999

This study was conducted to investigate the effects of Scoria, Quartz porphyry and Oak charcoal powder feeding by 1% level of concentrate on the fattening cross bred bulls(5/8 Korean Native Cattle, 2/8 Charolais, 1/8 Brahman germ plasma) Feeding trial was conducted with 4 treatment groups which were T1(Full feeding of concentrate and roughage for 12 months), T2(T1 and Scoria addition), T3(T1 and Quartz porphyry addition) and T4(Oak charcoal addition) for 360 days, consisting of 32 heads whose initially weights were about 299.7kg. The results obtained are summarized as follows; 1. During the over-all period, average daily gains by T3, T4, T2 and T1 were 1.024, 0.987, 0.977kg and 0.964, respectively, without a significant difference by treatments. 2. Concentrate intakes per body weight and TDN intakes required per unit of kilogram gain were lower in addition agent groups than in control, and in all the treatments feed efficiency was higher at early stage for 360 fattening day than at end stage. 3. Carcass weight, dressing percent, back-fat thickness and loin eye muscle area by treatments were 330.9 to 348.4kg(average 340.3kg), 55.2~56.2(average 55.8%), 0.41~0.55(average 0.46cm)and 76.0~80.9$\textrm{cm}^2$(average 77.6$\textrm{cm}^2$), respectively, and were improved in addition agent groups than in control. 4. Meat quality grade is higher in addition agent groups than in control, and heads of 1st grade by treatments was 3, 2, 1 and none in T4, T2, T3 and T1, respectively. 5. Incidence heads of diseases by treatment were not apparently different, but diseases of digestive system was decreased in addition agent groups than in control. 6. The nitrate nitrogen content of fattening bull dung which collected in rectum were 0.082~0.089% (average 0.084%), and the content in addition agent groups was smaller than in controll. 7. Income was higher in order of T4, T3, T2, and T1 and incomes of treatment groups were grater than that of control group by 21.4 to 33.5 percent. According to the above results it may be concluded that fattening bulls may be required to feed no more than 1% of Scoria, Quartz porphyry and Oak charcoal powder based on the concentrate feeding level during the fattening period(12 to 24 month of age) to produce high quality meat and increase income.

• 한국산림과학회지
• /
• 제34권1호
• /
• pp.1-14
• /
• 1977

지리산(智異山) 일대(一帶)에 생육(生育)되고 있는 잣나무집단(集團)의 변이(變異)를 구명(究明)하기 위해 수형(樹形), 침엽(針葉), 자엽(子葉), 종자(種子), 묘목성장(苗木成長)의 특성(特性)을 조사하였다. 두개 집단(集團)을 비교(比較) 검토(檢討)하였던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 침엽(針葉)의 엽장(葉長), 거치밀도(鋸齒密度), 종자충실율(種子充實率), 종자장(種子長), 종자후(種子厚), 자엽장(子葉長), 자엽폭(子葉幅), 자엽(子葉)의 기공선수(氣孔線數)와 묘고성장(苗高成長)에 있어서는 집단(集團), 개체간(個體間)에 유의차(有意差)를 인정(認定)할 수 있었다. 2. 수목(樹木)의 역지각(力枝角)과 목재(木材)의 비중(比重), 자엽(子葉)의 수지구수(樹脂溝數)의 형질(形質)에 있어서는 집단간(集團間)에 유의차(有意差)가 인정(認定)되었다. 3. 침엽(針葉)의 기공선수(氣孔線數), 종자폭(種子幅), 종자중량(種子重量), 자엽(子葉)의 거치밀도(鋸齒密度), 자엽수(子葉數)의 형질(形質)은 개체간(個體間)에 유의차(有意差)를 인정(認定)할 수 있었다. 4. 침엽(針葉)의 수지구(樹脂溝)는 집단(集團), 개체간(個體間) 모두 3개(個)의 주수지구(主樹脂溝)만 발달(發達)하여 변이폭(變異幅)은 인정(認定)할 수 없었다. 그리고 수간형(樹幹型)은 두 집단(集團) 모두 통직성(通直性)을 나타내었다. 5. 침엽(針葉)과 자엽(子葉) 형질(形質)의 변이계수(變異係數)는 대체로 자엽(子葉)의 것이 큰 경향(傾向)을 나타내었다.

• Clinics in Shoulder and Elbow
• /
• 제24권1호
• /
• pp.21-26
• /
• 2021

Background: To evaluate the demographics, clinical and radiographic features of calcific tendinitis of the shoulder in the Korean population, specifically focusing on the incidence of coexisting rotator cuff tear. Methods: Between October 2014 and January 2015, we performed a prospective multicenter study with 506 patients from 11 training hospitals in Korea. We collected data of demographics and radiographic analysis based on simple radiographs, clinical assessments based on visual analog scale (VAS) and the American Shoulder Elbow Surgeons (ASES) score, and treatment modalities that are used currently. We also evaluated coexisting rotator cuff tear by ultrasonography (US) or magnetic resonance imaging (MRI) images. Results: There were 402 female patients (79%) with mean age of 55 years (range, 31-87 years). Mean duration of symptoms was 16 months. Mean size of calcific materials was 11.4 mm (range, 0-35 mm). Mean value of VAS and ASES scores were 6.5 (range, 1-10) and 47 (range, 8-95), respectively. Of 383 patients (76%), 59 (15%) had rotator cuff tear including 15 full-thickness tears on US or MRI. Patients with rotator cuff tears were significantly associated with older age, recurrent symptoms, menstrual disorders in females, and having undergone calcification removal surgery and rotator cuff repair (all p<0.05). Conclusions: This study reported demographic, radiographic, and clinical features of calcific tendinitis of the shoulder in Korean population, which were not different from those of Western population. Coexisting rotator cuff tear was found with 15% incidence in this large series, suggesting that further radiographic study to evaluate rotator cuff tear might be needed in some calcific tendinitis patients of older age and presenting with recurrent symptoms.

• 한국방사선학회논문지
• /
• 제16권7호
• /
• pp.961-968
• /
• 2022

회전근개 파열은 성인 어깨 통증의 주요 원인이다. 사회활동의 증가로 인해 어깨 통증을 호소하는 환자가 증가하고 있으며, 어깨질환에 대한 관심도 높아지고 있다. 초음파 장비의 발달로 진단의 정확도가 높아지면서 근골격계 질환 중에서 회전근개 파열 진단에 주로 이용되면서 초음파검사는 회전근개 파열에서 MRI 검사를 보완할 수 있는 방법으로 인정받고 있다. 따라서 본 연구에서는 어깨 회전근개 파열의 진단에 있어 초음파와 MRI 검사의 진단적 유용성을 알아보고자 하였다. 초음파검사와 MRI검사를 완료한 후 관절내시경검사로 최종 회전근개의 손상을 진단받은 환자 262명을 대상으로 후향적 분석을 하였다. 회전근개중 파열의 빈도가 가장 높은 극상건과 견갑하건 두 힘줄의 초음파검사와 MRI검사의 결과에 대한 민감도, 특이도, 양성예측도, 음성예측도, 정확도를 분석하였다. 또한 회전근개파열의 정도를 5단계로 점수화하여 비교하였다. 초음파 검사는 전층파열과 부분파열 모두 MRI 검사 결과와 유사하였고 통계적으로 유의한 차이는 없었다. 부분파열 검사 결과는 MRI 검사보다 양성예측도와 정확도가 높았다. 결론적으로 초음파는 회전근개 질환을 검사하기 위한 선별검사로 충분히 활용될 수 있으며, 환자의 체질과 상황에 따라 선택하여 임상적으로 활용될 것으로 생각된다.

• 대한화상학회지
• /
• 제23권2호
• /
• pp.54-59
• /
• 2020

Purpose: A fourth degree burn is a full-thickness burn of the skin usually accompanied by damage to deep structures and commonly results in extensive damage to surrounding tissues which makes the treatment of the wound difficult. Coverage of these wound using free flap is known to effective but not commonly used. The purpose of our study is to review our experience and suggest early application of free flap surgery. Methods: A retrospective review was performed from 2010 to 2019, on a total of 34 fourth degree burn patients undergone free flap surgery as primary treatment in our hospital. We reviewed the location of the injury, etiology, TBSA (%), Presence of osteomyelitis, flap choice, complications, period of injury to surgery and healing. Results: Using free flap as a primary reconstrcuction, the outcome is satisfactory. The treatment period was shortened, and there was less loss of function due to complications. Also the incidence of osteomyelitis and amputation was significantly low. Conclusion: Applying free flap surgery as soon as possible in fourth degree burns is effective, such as reducing complications such as infection, reducing amputation, shortening treatment period, and preventing severe sequelae.

• Journal of Periodontal and Implant Science
• /
• 제53권4호
• /
• pp.306-317
• /
• 2023

Purpose: Biphasic calcium phosphate (BCP), a widely used biomaterial for bone regeneration, contains synthetic hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), the ratio of which can be adjusted to modulate the rate of degradation. The aim of this study was to evaluate the clinical and radiographic benefits of reconstructing peri-implant bone defects with a newly developed BCP consisting of 60% β-TCP and 40% HA compared to demineralized bovine bone mineral (DBBM). Methods: This prospective, multicenter, parallel, single-blind randomized controlled trial was conducted at the periodontology departments of 3 different dental hospitals. Changes in clinical (defect width and height) and radiographic (augmented horizontal bone thickness) parameters were measured between implant surgery with guided bone regeneration (GBR) and re-entry surgery. Postoperative discomfort (severity and duration of pain and swelling) and early soft-tissue wound healing (dehiscence and inflammation) were also assessed. Data were compared between the BCP (test) and DBBM (control) groups using the independent t-test and the χ² test. Results: Of the 53 cases included, 27 were in the test group and 26 were in the control group. After a healing period of 18 weeks, the full and mean resolution of buccal dehiscence defects were 59.3% (n=16) and 71.3% in the test group and 42.3% (n=11) and 57.9% in the control group, respectively. There were no significant differences between the groups in terms of the change in mean horizontal bone augmentation (test group: -0.50±0.66 mm vs. control groups: -0.66±0.83 mm, P=0.133), postoperative discomfort, or early wound healing. No adverse or fatal complications occurred in either group. Conclusions: The GBR procedure with the newly developed BCP showed favorable clinical, radiographic, postoperative discomfort-related, and early wound healing outcomes for peri-implant dehiscence defects that were similar to those for DBBM.

• 콘크리트학회논문집
• /
• 제21권3호
• /
• pp.347-358
• /
• 2009

최근에 들어서 국내에서도 무량판 구조 (flat plate slab)가 적용될 수 있는 여건이 마련되어가고 있다. 그러나 무량판 구조는 보가 없는 구조이기 때문에 일반적인 라멘구조형식에 비하여 처짐 제어가 어려우며, 포스트텐션 (post-tension) 공법은 이러한 문제점을 극복하기 위한 매우 효율적인 방법이다. 포스트텐션 구조를 적용하면 슬래브 두께의 증가 없이도 효율적으로 슬래브의 처짐을 제어 할 수 있다. 그러나, 이러한 장점에도 불구하고, 국내에서 포스트-텐션의 적용은 비용증가, 기술적인 문제, 경험부족 등으로 인하여 그 적용이 매우 제한적이다. 따라서 전구간 포스트텐션 (full-PT)에 대한 부담을 줄이고 나아가 기존 포스트텐션 구조형식의 시공성을 향상하기 위하여 본 연구에서는 슬래브 내부에서 긴장작업을 수행할 수 있는 상향긴장식 부분PT공법을 제안하였다. 이러한 상향긴장방식은 정착구를 필요에 따라 다양한 위치에 설치할 수 있기 때문에 처짐 효율에 대한 분석이 필요하며, 정착구 후면에서 인장응력을 야기시키는 문제점이 있다. 따라서, 본 연구에서는 상향긴장식 부분PT공법 적용할 때 정착구 위치에 따른 처짐효율분석, 유한요소해석 및 스트럿-타이를 이용한 인장응력 또는 인장력 해석 등을 실시하였으며, 상향긴장식 부분PT공법을 실제로 현장에 적용하고, 계측결과를 해석결과와 비교 분석함으로써 현장적용성을 알아보았다. 상향긴장식 부분PT공법은 처짐 또는 인장응력이 크게 발생되는 부분 구간에만 선택적으로 적용하면 적은 비용증가로 큰 처짐효율을 얻어낼 수 있음을 확인하였다.

• 원예과학기술지
• /
• 제33권2호
• /
• pp.186-195
• /
• 2015

본 연구는 $GA_3$와 TDZ 처리에 따른 '흑보석' 포도의 품질 특성변화와 과실 연화 지연을 위한 생장조절제의 효과를 구명하기 위하여 실시하였다. 최종생산량은 저농도 $GA_3$ 단용처리에서 목표생산량 대비 88.3%로 낮았으나 TDZ를 2차에 혼용함으로써 최종생산량은 회복되었다. 착색을 기준으로 수확하였을 때 만개 후 100일까지의 수확률은 $GA_3$ 단용처리시 낮은 수확률을 보였으며 TDZ를 혼용할 경우 상쇄되었고 특히 2차에 혼용할 경우 효과적이었다. 반면에 생장조절제 처리시 가용성 고형물 함량은 만개 후 90일 이후에는 큰 변화가 없었으며 산 함량은 만개 후 90일부터 급격히 감소되어 당도와 산도를 기준으로 할 경우 오히려 무처리구에 비해 일찍 성숙기에 도달하였다. 경도는 $GA_3$와 TDZ 처리에 의해 활발한 세포비대와 세포분열로 과피가 두꺼워지고 밀도가 증가함에 따라 무처리구에 비해 만개 후 최종 수확시까지 높게 유지되었으며 특히 1, 2차 $GA_3$ $2.5mg{\cdot}L^{-1}$ + TDZ $2.5mg{\cdot}L^{-1}$ 처리구에서 연화가 지연되어 과실의 품질을 개선할 수 있을 것으로 생각되었다. 무핵과실은 TDZ를 혼용시에도 가장 높은 무핵률이 65.5%에 불과하여 무핵유기가 어려웠으며 열과발생은 모든 처리구에서 0.0-0.9%로 거의 발생하지 않았다. 따라서 '흑보석' 포도는 1, 2차 $GA_3$ $2.5mg{\cdot}L^{-1}$ + TDZ $2.5mg{\cdot}L^{-1}$로 처리하여 경도가 급격히 낮아지기 전인 만개 후 100일 이전에 수확하는 것이 적절할 것으로 생각되었다.