• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.563-569
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• 2004

The DNA sequence of the chitosanase gene (choK) from $\beta$-Proteobacterium KNU3 showed an 1,158-bp open reading frame that encodes a protein of 386 amino acids with a novel 74 signal peptide. The degenerated primers based on the partial deduced amino acid sequences from MALDI- TOF MS analyses yielded the 820 bp of the PCR product. Based on this information, double inverse PCR cloning experiments, which use the two specific sets of PCR primers rather than single set primers, identified the unknown 1.2 kb of the choK gene. Subsequently, a 1.8 kb of full choK gene was cloned from another PCR cloning experiment and it was then subcloned into pGEM T-easy and pUC18 vectors. The recombinant E. coli clone harboring recombinant pUC18 vector produced a clear halo around the colony in the glycol chitosan plates. The recombinant ChoK protein was secreted into medium in a mature form while the intracellular ChoK was produced without signal peptide cleavage. The activity staining of PAGE showed that the recombinant ChoK protein was identical to the chitosanase of wild-type. The comparison of deduced amino acid sequences of choK revealed that there is 92% identity with that of Sphingobacterium multivorum chitosanase. Judging from the conserved module in other bacterial chitosanases, chitosanase of KNU3 strain (ChoK) belongs to the family 80 of glycoside hydrolases.

• Structural Engineering and Mechanics
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• v.56 no.1
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• pp.27-47
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• 2015

In this study, the accuracy and reliability of fully nonlinear method against equivalent linear method for dynamic analysis of soil-structure interaction is investigated comparing the predicted results of both numerical procedures with the results of experimental shaking table tests. An enhanced numerical soil-structure model has been developed which treats the behaviour of the soil and the structure with equal rigour. The soil-structural model comprises a 15 storey structural model resting on a soft soil inside a laminar soil container. The structural model was analysed under three different conditions: (i) fixed base model performing conventional time history dynamic analysis, (ii) flexible base model (considering full soil-structure interaction) conducting equivalent linear dynamic analysis, and (iii) flexible base model performing fully nonlinear dynamic analysis. The results of the above mentioned three cases in terms of lateral storey deflections and inter-storey drifts are determined and compared with the experimental results of shaking table tests. Comparing the experimental results with the numerical analysis predictions, it is noted that equivalent linear method of dynamic analysis underestimates the inelastic seismic response of mid-rise moment resisting building frames resting on soft soils in comparison to the fully nonlinear dynamic analysis method. Thus, inelastic design procedure, using equivalent linear method, cannot adequately guarantee the structural safety for mid-rise building frames resting on soft soils. However, results obtained from the fully nonlinear method of analysis fit the experimental results reasonably well. Therefore, this method is recommended to be used by practicing engineers.

• Coupled systems mechanics
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• v.1 no.4
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• pp.361-379
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• 2012

This paper presents a fully coupled three-dimensional solver for the analysis of interaction between pulsatile flow and large deformation structure. A partitioned time marching algorithm is employed for the solution of the time dependent coupled discretised problem, enabling the use of highly developed, robust and well-tested solvers for each field. Conservative transfer of information at the fluid-structure interface is combined with an effective multi-predict-correct iterative scheme to enable implicit coupling of the interacting fields at each time increment. The three-dimensional unsteady incompressible fluid is solved using a powerful implicit time stepping technique and an ALE formulation for moving boundaries with second-order time accurate is used. A full spectrum of total variational diminishing (TVD) schemes in unstructured grids is allowed implementation for the advection terms and finite element shape functions are used to evaluate the solution and its variation within mesh elements. A finite element dynamic analysis of the highly deformable structure is carried out with a numerical strategy combining the implicit Newmark time integration algorithm with a Newton-Raphson second-order optimisation method. The proposed model is used to predict the wave flow fields of a particular flow-induced vibrational phenomenon, and comparison of the numerical results with available experimental data validates the methodology and assesses its accuracy. Another test case about three-dimensional biomedical model with pulsatile inflow is presented to benchmark the algorithm and to demonstrate the potential applications of this method.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1473-1477
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• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.

• Cartoon and Animation Studies
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• s.11
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• pp.11-28
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• 2007

The article concerns the public policy for the development of the arts educator in the field of the comic and animation education. The comic and animation education programs are focused on the promotion of the creativity and communication skills of the student in terms of the arts education. The article seeks to examine the case on the support program for the comic and animation educators, which has been managed by Korea Arts & Culture Education Service(KACES) since 2005. The forty two educators were surveyed. As a result, the support program of the KACES has an effect on the educator's recognition of the comic and animation education in the positive way. The most of the educators suggest that the support program should try to develop the comic and animation educator job as a special work with the full time job. In this repect, the article recommend the policy alternatives such as the promotion of the education program R & D, the knowledge and information system, training system and the establishment of the governance system.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1997.06c
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• pp.89-98
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• 1997

This study was conducted to develop an automatic nutrient-solution control system for small-scale growers. The nutrient-solution control system consisted of a low-cost and precise metering device and a personal computer. The system controlled electric conductivity(EC) and pH of nutrient-solution based on the time-based feedback control method with the information about temperature, EC, and pH of the nutrient-soIution. The performance of the nutrient-solution control system was evaluated through the control of EC and pH while compared with those of commercial nutrient-solution control system. Also an experimental cultivation of tomato was conducted to verify and to improve the developed system. Results of this study were as follows. 1. An automatic nutrient-solution control system based on a low-cost and precise metering device was developed. 2. The developed system controlled EC and pH within $\pm$0.05 mS/cm and $\pm$0.2 pH full scale error respectively at $24^{/circ}C$. 3. When using the commercial system, the controlled values of EC and pH of the 500l of water were 1.29 mS/cm and 6.1 pH for the setting points of 1.4 mS/cm and 6.0 pH respectively at $22^{/circ}C$. 4. The developed nutrient-solution control system showed $\pm$0.05 mS/cm of deviation from the setting EC value over the experimental cultivation period. 5. The deviation from the average values of Ca and Mg mass content in the several nutrient-solution were 0.5% and 1.8% respectively.

• Korean Journal of Animal Reproduction
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• v.11 no.3
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• pp.189-195
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• 1987

This study was conducted in order to obtain the information of the histological changes in each of six segments of the epididymal ducts in Korean native goats. Thirty-two Korean native male goats were examined, dividing into seven groups, at 4 weeks intervals from 8 to 32 weeks of age. The results obtained were as follows. 1. The epididymal ducts showed histologically an abrupt growth at the age of 16 weeks being followed by almost full maturation at the age of 24 weeks. Diameter of the cauda was steadily larger than that of the caput and corpus of the epididymal ducts. 2. Spermatozoa in the lumen of epididymal ducts were first observable at the age of 16 weeks, thereafter showing sparse in the lumen of caput, whereas most dense in the lumen of cauda in the density of spermatozoa. 3. Ducts in the caput and corpus were lined by ciliated columnar epithelium until the age of 12 weeks, and later by pseudostratified ciliated columnar epithelium which was composed of ciliated columnar cells, clear cells and basal cells. Ducts of cauda epididymis were lined by simple ciliated columnar epithelia until 12 weeks of age and later by simple or pseudostratified ciliated columnar epithelium, and two types of ducts (small ducts with high epithelium and large ducts with lower epithelium) were noted. Nucleus of the epithelial cells in the caput were located in the base of cells but in the corpus and cauda, those were located in the mid part of cells. cilia were most developed in the epithelia of the corpus.

• The Journal of the Korea Contents Association
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• v.9 no.5
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• pp.174-181
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• 2009

Recently hospitals are implementing a One Stop Service as part of patient-care service. With the One Stop Service, medical treatment including consultation, inspection, and results are changing to be made available in one visit. Therefore most examinations are reserved for the same day; however, there are cases in which additional visits are necessary because of certain properties related to exams. This study compares and analyzes the number of reinspections before and after reforms. By designating the order of priority for BMD examinations and implementing education from information obtained in the OCS E-manual update, the number of reinspections for wards was reduced from 58 to 21, Outpatient departments were reduced from 51 to 12, and errors in reservations made by employees in the department of radiology were reduced from 98 to 11. Reinspections can be reduced with full understanding the factors related to reinspection and a background check prior to inspection in order to determine the order of priority for inspections. This will also reduce workers' stress and increase their efficiency and at the same time decease patient dissatisfaction and improve hospital reliability.

• The Journal of the Korea Contents Association
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• v.9 no.7
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• pp.9-18
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• 2009

This paper deals with the elderly serious game design which makes full use of the existing arcade game machine. This serious game is on the issue for its positive role recently. Serious arcade game for the elderly which is made up arcade platform-based combined contents manufacture technology has an element which is having a fun for building up physical and mental health. It also has a special objective which can prevent against senile dementia. Above-mentioned serious arcade game which has a special objective in both hardware part and software part is needed to study how to design. Accordingly, this paper presents how to make up special-purpose entertainment machine and contents development by biologic, social, and psychological researches and data about the elderly - main users. We hope that this paper can be used as a base for expansion of new industrial field by integrating new product development for the elderly with high game technology.With the help of information technology

• Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.43-54
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• 2011

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.