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Full-Length Enriched cDNA Library Construction from Tissues Related to Energy Metabolism in Pigs

  • Lee, Kyung-Tai;Byun, Mi-Jeong;Lim, Dajeong;Kang, Kyung-Soo;Kim, Nam-Soon;Oh, Jung-Hwa;Chung, Chung-Soo;Park, Hae-Suk;Shin, Younhee;Kim, Tae-Hun
    • Molecules and Cells
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    • v.28 no.6
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    • pp.529-536
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    • 2009
  • Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.

Evaluation of Color Reproduction Characteristics of TFT-LCD and AMOLED for Mobile Phone (모바일폰용 TFT-LCD와 AMOLED의 색재현성 평가)

  • Park, Tae-Yong;Lee, Cheol-Hee;Ha, Yeong-Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.45 no.1
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    • pp.29-37
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    • 2008
  • With the rapid development of display technology as well as the diversity of display, image quality assessment plays an important role in display color reproduction. The image quality has described by objective evaluation based on the physical measured data, such as contrast ratio, color reproduction range, color temperature, and gamma characteristics, etc., however, the final judgement is achieved by perceptual quality assessment of observers. Therefore, it is necessary to investigate the relationship between physical performance of display and perceptual image quality. Thus, in order to evaluate objectively the color reproduction characteristics of two mobile displays, TFT-LCD and AMOLED, we measured the darkroom contrast ratio of full screen, luminance and color temperature of full-screen white, full-screen gray and color linearity, tone reproduction of color scales for each RGB primary, and color reproduction range. Then the pair-wise comparison is implemented for image experts and naive observers not only to judge their preference on principal evaluation attributes but also to subjectively describe perceived image quality. Through two evaluation processes, we derived the objective bases that can prove the results of subjective image quality assessment by interconnecting physical factors that have influence on observers' preference judgements. Finally, this paper provided important information for improving the image quality and increasing preference from an observer's point of view.

Full-mouth rehabilitation of severely attrited dentition with missing posterior teeth: a case report using digital workflow with jaw motion tracking (심한 교모와 구치부 상실을 보이는 환자의 전악 수복: Jaw motion tracking과 digital workflow를 활용한 증례 보고)

  • Chan Young Park;Younghoo Lee;Seoung-Jin Hong;Janghyun Paek;Kwantae Noh;Ahran Pae;Hyeong-Seob Kim;Kung-Rock Kwon
    • The Journal of Korean Academy of Prosthodontics
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    • v.61 no.4
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    • pp.293-307
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    • 2023
  • Jaw motion tracking, which is introduced in recent case reports, is a method which records the patient's individualized pathway of the mandibular movements along with facebow transfer, and reproduces the information in the virtual space of computer-aided-design/computer-aided-manufacturing (CAD-CAM) software. In this present case, a collapse of the occlusal plane was observed, due the loss of posterior teeth for a long period. Full-mouth rehabilitation with an increase in the occlusal vertical dimension was planned. First, the patient's mandibular movements were recorded on the newly established jaw relation by jaw tracking, and this information was assembled with the patient's intraoral data to create a virtual patient. Implant planning and diagnostic wax-up was done on the virtual patient, leading the fabrication of the provisional prosthesis. On the newly established jaw relation with an increase in the occlusal vertical dimension, canine guidance of the provisional prosthesis was checked. Finally, the provisional prosthesis was carried out to the definitive prosthesis. Using the advantages of the technologies in the digital dentistry, the patient was satisfied with the function and the esthetics after the treatment.

A Study of Statistic Behavior of Segmental U-shaped Prestressed Concrete Girder Applied with Integrated Tensioning Systems (복합긴장방식이 적용된 세그멘탈 U형 거더 정적 거동 연구)

  • Hyunock Jang;Ilyoung Jang
    • Journal of the Society of Disaster Information
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    • v.20 no.2
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    • pp.329-338
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    • 2024
  • Purpose: This study verified the safety of the improved box-type girder behavior by comparing and evaluating the bending behavior results of a full-scale specimen based on the analytical behavior of the splice element PSC U-shaped girder with integrated tensioning systems. Method: Based on the results of the service and strength limit state design using the bridge design standard(limit state design method), the applied load of a 40m full-scale specimen was calculated and a static loading experiment using the four-point loading method was performed. Result: When the design load, crack load, and ultimate load were applied, the specimen deflection occurred at 97.1%, 98.5%, and 79.0% of the analytical deflection value. When the design load, crack load, and ultimate load were applied, the crack gauge was measured at 0.009~0.035mm, 0.014~0.050mm, and 6.383~5.522mm at each connection. Conclusion: The specimen behaved linear-elastically until the crack load was applied, and after cracks occurred, it showed strainhardening up to the ultimate load, and it was confirmed that the resistance of bending behavior was clearly displayed against the applied load. The cracks in the dry joints were less than 25% of grade B based on the evaluation of facility condition standard. The final residual deformation after removing the ultimate load was 0.114mm, confirming the stable behavior of the segment connection.

A Study on the Improvements of the Speech Quality by using Distribution Characteristics of LSP parameters in the EVRC(Enhanced Variable Rate Codec) (LSP 파라미터의 분포특성을 이용한 EVRC의 음질개선에 관한 연구)

  • Min, So-Yeon;Na, Deok-Su
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.12
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    • pp.5843-5848
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    • 2011
  • To improve the efficiency of the channel spectrum and to reduce the power consumption of the system in EVRC, the voice signal is compressed and transmitted only when the user speaks to. In addition to this, voice frames are divided into three rates 1, 1/2 and 1/8 and each frame is handled differently. For example, we assumed that the input is silence region if the 1/8 rate is used. In this paper, the sections are firstly separated into the voiced speech signal region, unvoiced speech signal region, and silence region by using distribution characteristics of LSP parameters. Then the paper suggested to encode 1 rate for the voiced speech signal, 1/2 rate for the unvoiced speech signal region, 1/8 rate for the silence region. In other words, traditional way of transmission is used when sending full rate in the EVRC. However, when sending half rate, the voice is firstly distinguished between voiced and unvoiced. If the voice is distinguished as voiced, voice is converted into full rate before the transmission. If it is distinguished as silence, EVRC's basic rate is applied. In the experimental results with SNR, ASDM, transmission bit rate measurement, we have demonstrated that voice quality was improved by using the proposed algorithm.

Application of Effective Regularization to Gradient-based Seismic Full Waveform Inversion using Selective Smoothing Coefficients (선택적 평활화 계수를 이용한 그래디언트기반 탄성파 완전파형역산의 효과적인 정규화 기법 적용)

  • Park, Yunhui;Pyun, Sukjoon
    • Geophysics and Geophysical Exploration
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    • v.16 no.4
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    • pp.211-216
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    • 2013
  • In general, smoothing filters regularize functions by reducing differences between adjacent values. The smoothing filters, therefore, can regularize inverse solutions and produce more accurate subsurface structure when we apply it to full waveform inversion. If we apply a smoothing filter with a constant coefficient to subsurface image or velocity model, it will make layer interfaces and fault structures vague because it does not consider any information of geologic structures and variations of velocity. In this study, we develop a selective smoothing regularization technique, which adapts smoothing coefficients according to inversion iteration, to solve the weakness of smoothing regularization with a constant coefficient. First, we determine appropriate frequencies and analyze the corresponding wavenumber coverage. Then, we define effective maximum wavenumber as 99 percentile of wavenumber spectrum in order to choose smoothing coefficients which can effectively limit the wavenumber coverage. By adapting the chosen smoothing coefficients according to the iteration, we can implement multi-scale full waveform inversion while inverting multi-frequency components simultaneously. Through the successful inversion example on a salt model with high-contrast velocity structures, we can note that our method effectively regularizes the inverse solution. We also verify that our scheme is applicable to field data through the numerical example to the synthetic data containing random noise.

Analysis on Reactions of Full-Scale Airframe Static Structural Test (항공기 전기체 정적구조시험의 반력 분석)

  • Shim, Jae-yeul
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.48 no.3
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    • pp.195-205
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    • 2020
  • This study addresses analysis on reactions which are induced in restraint system for airframe full-scale static structural test. This system restraints 6 degrees of freedom of a test article. It is valuable to study evaluating test error through analysis on the reactions which include all errors in a test. It is required to calculate fistly right reactions for the evaluation. This study focuses on calculation of the right reactions. The reaction is represented by sum of nominal reaction(Rn) and testing error reactions(Rce, Rerr) and is analyzed by two steps (inital vs relative reaction) in this study. It would evaluate intrinsic error at 0%DLL and error induced from applying test load, separately. Based on analysis using test data of a full-scale static test(canard type aircraft), resultant force of Rces and Rce_rs are distributed within 82.8N while resultant force of Rerr_rs shows to increase upto max. 808N as load level increment. Such well distribution of the Rce within the small range is caused from TMF values characteristics which are well distributed within -30N~40N. Additionally, it is shown through qualitative analysis on three components(X0, Y0, Z0) of the relative reaction(Rerr_r) that the reactions must be calculated with considering deformation of test article to calculate correctly reactions. This study shows also that equations characterizing deformation of components of test article are required to calculate the correct reactions, the equations must include information which will be used to calculate movement of all loading points.

A Novel Design of a Low Power Full Adder (새로운 저전력 전가산기 회로 설계)

  • Kang, Sung-Tae;Park, Seong-Hee;Cho, Kyoung-Rok;You, Young-Gap
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.38 no.3
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    • pp.40-46
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    • 2001
  • In this paper, a novel low power full adder circuit comprising only 10 transistors is proposed. The circuit is based on the six -transistor CMOS XOR circuit, which generates both XOR and XNOR signals and pass transistors. This adder circuit provides a good low power characteristics due to the smaller number of transistors and the elimination of short circuit current paths. Layouts have been carried out using a 0.65 ${\mu}m$ ASIC design rule for evaluation purposes. The physical design has been evaluated using HSPICE at 25MHz to 50MHz. The proposed circuit has been used to build 2bit and 8bit ripple carry adders, which are used for evaluation of power consumption, time delay and rise and fall time. The proposed circuit shows substantially improved power consumption characteristics, about 70% lower than transmission gate full adder (TFA), and 60% lower than a design using 14 transistors (TR14). Delay and signal rise and fall time are also far shorter than other conventional designs such as TFA and TR14.

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A Study on Motion Estimator Design Using DCT DC Value (DCT 직류 값을 이용한 움직임 추정기 설계에 관한 연구)

  • Lee, Gwon-Cheol;Park, Jong-Jin;Jo, Won-Gyeong
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.38 no.3
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    • pp.258-268
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    • 2001
  • The compression method is necessarily used to send the high quality moving picture that contains a number of data in image processing. In the field of moving picture compression method, the motion estimation algorithm is used to reduce the temporal redundancy. Block matching algorithm to be usually used is distinguished partial search algorithm with full search algorithm. Full search algorithm be used in this paper is the method to compare the reference block with entire block in the search window. It is very efficient and has simple data flow and control circuit. But the bigger the search window, the larger hardware size, because large computational operation is needed. In this paper, we design the full search block matching motion estimator. Using the DCT DC values, we decide luminance. And we apply 3 bit compare-selector using bit plane to I(Intra coded) picture, not using 8 bit luminance signals. Also it is suggested that use the same selective bit for the P(Predicted coded) and B(Bidirectional coded) picture. We compare based full search method with PSNR(Peak Signal to Noise Ratio) for C language modeling. Its condition is the reference block 8$\times$8, the search window 24$\times$24 and 352$\times$288 gray scale standard video images. The result has small difference that we cannot see. And we design the suggested motion estimator that hardware size is proved to reduce 38.3% for structure I and 30.7% for structure II. The memory is proved to reduce 31.3% for structure I and II.

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Development of an Edge-based Point Correlation Algorithm Avoiding Full Point Search in Visual Inspection System (전탐색 회피에 의한 고속 에지기반 점 상관 알고리즘의 개발)

  • Kang, Dong-Joong;Kim, Mun-Jo;Kim, Min-Sung;Lee, Eung-Joo
    • The KIPS Transactions:PartB
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    • v.11B no.3
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    • pp.327-336
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    • 2004
  • For visual inspection system in real industrial environment, it is one of most important tasks to design fast and stable pattern matching algorithm. This paper presents an edge-based point correlation algorithm avoiding full search in visual inspection system. Conventional algorithms based on NGC(normalized gray-level correlation) have to overcome some difficulties for applying to automated inspection system in factory environment. First of all, NGC algorithms need high time complexity and thus high performance hardware to satisfy real-time process. In addition, lighting condition in realistic factory environments if not stable and therefore intensity variation from uncontrolled lights gives many roubles for applying directly NGC as pattern matching algorithm in this paper, we propose an algorithm to solve these problems from using thinned and binarized edge data and skipping full point search with edge-map analysis. A point correlation algorithm with the thinned edges is introduced with image pyramid technique to reduce the time complexity. Matching edges instead of using original gray-level pixel data overcomes NGC problems and pyramid of edges also provides fast and stable processing. All proposed methods are preyed from experiments using real images.