• Development and Reproduction
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• v.4 no.2
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• pp.161-173
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• 2000

The aim of this study was to assess the effect of the frequency of the L$N_2$ infusion on the ultrastructure, metabolism and development of the embryo after freezing and thawing by computerized cell freezer. Two-cell embryos of ICR mouse were randomly allocated into fresh (control), high-frequency freezing (group 1) and low-frequency freezing (group 2). For fresh and frozen-thawed intact 2-cell embryos, total ceil number in the blastocyst was counted by fluorescent microscope after Hoechst 33258 staining. Relative amount of $H_2O$$_2$ was measured by DCHFDA. Intracellular location and membrane potential of the mitochondria were evaluated by staining with rhodamine 123 and JC-1. The structure of actin filament was also evaluated by confocal microscope. DNA fragmentation was assessed by TUNEL method after development into blastocyst. The survival rate of intact embryo was higher in group 1 than group 2 (50.7％ vs. 34.6％ respectively, p<0.05). The blastocyst developmental rate was significantly low in group 2 (86.7％, 76.7％ vs. 44.0％ for control, group 1 and group 2 respectively, p<0.05). Total cell number in the blastocyst was also significantly lower in group 2 than control (79.5$\pm$12.9, 71.6$\pm$8.0, and 62.5$\pm$4.7 for control, group 1 and group 2 respectively, p<0.05). The relative amount of $H_2O$$_2$ was higher in group 2 than other groups (15.3$\pm$3.0, 16.6$\pm$1.6 vs. 23.4$\pm$1.8, p<0.05). After JC-1 staining, relative intensity of mitochondria with high membrane potential was significantly lower in group 2 than control and group 1 (17.2$\pm$3.8, 17.4$\pm$1.3 vs. 13.2$\pm$2.0, p<0.05). In group 2, partial deletion and aggregation of the actin filament was found. DNA fragmentation rate was also hieher for group 2 versus other groups (30.8％, 36.0％ vs. 65.6％, p<0.05). The frequency of the L$N_2$ infusion is an important factor for the development of frozen-thawed mouse embryo. High-frequency infusion may prevent damages of cytoskeleton and mitochondria in the embryo probably by preventing the temperature fluctuation during dehydration phase. We speculate that the application of high-frequency infusion method in human embryo may be promising.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.9-15
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• 2016

We investigated >$50-{\mu}m$ marine planktonic organisms (mainly zooplankton) using a bongo net in Masan Bay and Jangmok Bay in order to harvest 75% of natural communities based on Phase-II approval regulations by the United States Coast Guard (USCG). The concentrated volume (in 1 ton) and abundance of zooplankton were $1.8{\times}10^7ind.ton^{-1}$ and $2.3{\times}10^7ind.ton^{-1}$, and their survival rates were 82.6% and 80.1%, respectively. The community structure in Jangmok Bay was similar to that in Masan Bay, and dominant species were adult and immature groups (stage IV) of genus Acartia. Harvested populations were inoculated in a 500-ton test tank. Although the population abundances were $6.0{\times}10^4ind.ton^{-1}$ for both bay samples, the mortality rates were higher in the Masan Bay population (32%) than the Jangmok Bay population (20%). We considered the reason to be that there were 30% more immature individuals of Acartia from Masan Bay than from Jangmok Bay. The younger population may have been greatly stressed by the moving process and netting gear. After applying a Ballast Water Treatment System (BWTS) using a sample form Jangmok Bay, the mortality rates in the treatment groups were found to be 100% after 0 days and 5 days, implying that the BWTS worked well. During the winter season, the zooplankton concentration method alone did not easily satisfy the approval standards of USCG Phase II (> $10{\times}10^4ind.ton^{-1}$ in the 500 ton tank). Increasing the netting frequency and additional fishing boats may be helpful in meeting the USCG Phase II biological criteria.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.10
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• pp.188-198
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• 2013

Maximally flat (MAXFLAT) half-band filters usually have wider transition band than other filters. This is due to the fact that the maximum possible number of zeros at $z={\pm}1$ is imposed, which leaves no degree of freedom, and thus no independent parameters for direct control of the frequency response. This paper describes a novel method for the design of FIR halfband filters with an explicit control of the transition-band width. The proposed method is based on a generalized Lagrange halfband polynomial (g-LHBP) with coefficients parametizing a 0-th coefficient $h_0$, and allows the frequency response of this filter type to be controllable by adjusting $h_0$. Then, $h_0$ is modeled as a steepness parameter of the transition band and this is accomplished through theoretically analyzing a polynomial recurrence relation of the g-LHBP. This method also provides explicit formulas for direct computation of design parameters related to choosing a desired filter characteristic (by trade-off between the transition-band sharpness and passband & stopband flatness). The examples are shown to provide a complete and accurate solution for the design of such filters with relatively sharper transition-band steepness than MAXFLAT half-band filters.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.9 s.112
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• pp.900-912
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• 2006

A GPS receiving antenna installed in the missile's warhead is designed and fabricated at a center frequency 1.575 GHz. The circular shaped antenna is installed in the middle of the warhead where the antenna's patch and the ground plane are connected with a hollow cylindrical shaped short pin. Using the dual feeding and phase difference method, an omni-directional radiation pattern which direction is normal to the missile's axis(H-plane) is obtained. The optimized diameters of the circular patch and the cylindrical ring typed shorting pin of the GPS receiving antenna which use the FR4.material(dielectric constant $\varepsilon_r=4.6$) are 59.5 mm and 14 mm, respectively. The cylindrical body with diameter 100 mm and height 500 mm is attached to the lower part of the warhead in order to complete the missile figure. The radiation patterns are measured by changing the angle and phase between the dual feeding points. When the phase difference of dual feeding is $100^{\circ}$ and the angle between the dual feeding points is $100^{\circ}$, the nearly omni-directional radiation pattern in the H-plane is obtained. In this case, the antenna gain is -5.55 dBd and the relative level difference between the maximum and the minimum radiation intensity is 3.98 dB.

• Journal of Korean Academy of Nursing Administration
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• v.6 no.1
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• pp.123-134
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• 2000

This study intended to analyze the effectiveness to obtain by developing the critical pathway presented as the method to improve the quality-betterment and cost effectiveness the through the continuous and consistent patient management for the hysterectomy patient and applying it to the real practice. This study was designed to develop and effect the critical pathway for hysterectomy patients in the way to be possible the intergrated in patient management. It was adopted the process of seven phase to develop a critical pathway. To analyze the application effect of the developed critical pathway, this author offered health care service applying the critical pathway to the hysterectomy patient from July. 20 to Oct. 19. 1999. The study method had been done by investigating the experimental group and control group through the questionnaire on 40 patients who had been inpatient hysterectomy. Dependent variables were measured by modified from satisfaction, and cost and length of hospital stay. The data anlyzed by frequency, x2-test, t-test. The results of this study was as follows; 1. It was decided that the vertical line of the critical pathway was made up of eight items such as monitoring/assessment, treatment, activity, medication, consult. Lab test, diet, patient teaching and horizontal line was 7days from admission to discharge. 2. After the verifying the validity of the expert group about the critical pathway, the horizontal line was amended from hospitalization to five postoperative days and taking their consensus, some contents of the horizontal line was amended and deleted. 3. There was no significant differences in the experimental group and control group in the satisfaction, and significant differences in the cost, the length of hospital stay.

• Journal of Broadcast Engineering
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• v.19 no.2
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• pp.195-204
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• 2014

In this paper, we propose an automatic modulation classification method for ten digitally modulated baseband signals, such as 2-FSK, 4-FSK, 8-FSK, MSK, BPSK, QPSK, 8-PSK, 16-QAM, 32-QAM, and 64-QAM based on higher order statistics of cyclostationary process. The first order cyclic moments and higher order cyclic cumulants of the signal are used as features of the modulation signals. The proposed method consists of two stages. At the first stage, we classify modulation signals as M-FSK and non-FSK using peaks of the first order cyclic moment. At the next step, we apply the Gaussian mixture model-based classifier to classify non-FSK. Simulation results are demonstrated to evaluate the proposed scheme. The results show high probability of classification even in the presence of frequency and phase offsets.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.38 no.7
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• pp.22-27
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• 2001

In this paper, the complex permittivity of various dielectrics such as powder(sugar and flour) and solid(teflon and acrvl) are measured by using an open ended coaxial-line probe, which is self designed and manufactured, The probe is connected to a vector network analyzer(VNA) through a coaxial cable, The end of the cable is corrected by using an OSL(open, short, and load) calibration kit, The phase difference, which is produced by inserting the probe at the end of the line. is compensated by using the numerically calculated reflection coefficient of distilled water, The complex permittivity is reconstructed by inserting the measured reflection coefficient, which is produced at the interface between the probe and measuring material, into ,an virtual conical cable conversion model. Over a wide frequency range from 30 MHz to 3 GHz, the measured complex permittivitis of various powder and solid using the our method are compared with the results, which are measured by using an transmission-line method of the Korea Research Institute of Standards and Science(KRISS).

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.4
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• pp.436-441
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• 2008

In this paper, we derived the Y-parameter of the recently reported Balun-BPF with a dual mode ring resonator by using the network analysis method. Using the derived Y-parameter we verified that the Balun-BPF structure satisfy the balun conditions. In order to design the Balun-BPF with arbitrary characteristics using the standard BPF design method with ease we derived the immittance slope parameters of the dual mode ring resonator with a load. The notch frequencies are also predicted. By designing with the proposed equations, simulating with a circuit simulator, fabricated and measured a Balun-BPF we confirmed the validation of the equations. The measured data shows bandwidth of 71 MHz and insertion loss of 0.92 dB at the center frequency of 2.46 GHz. It shows the phase imbalance of $174{\sim}181$ degree and the magnitude imbalance within 0.5 dB.

• Journal of Biomedical Engineering Research
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• v.30 no.4
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• pp.333-340
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• 2009

MR compatible coaxial-slot antenna for microwave hyperthermia was developed while its structure and size of each part were determined by computer simulation using finite element method(FEM). Its local heating performance was evaluated using tissue-mimic phantom and swine muscles. 2% agarose gel mixed with 6mM/$\ell$ $MnCl_2$ as a biological tissue-mimic phantom was heated by the proposed antenna driven by a 2.45GHz microwave generator. The temperature changes of the phantom were monitored using multi-channel digital thermometer at the distance of 0mm, 5mm, 10mm and 20mm from the tip center of the antenna. Also muscle tissue of swine was heated for 2 and 5minutes with 50W and 30W of microwave generator powers, respectively, to evaluate the local heating performance of the antenna. MRI compatibility was also verified by acquiring MR images and MR temperature map. MR signals were acquired from the agarose gel phantom using $T2^*$ GRE sequence with 1.5T clinical MRI scanner(Signa Echospeed, GE, Milwaukee, WI, U.S.A.) at Pusan Paik Hospital and were transferred to PC in order to reconstruct MR images and temperature map using proton resonance frequency(PRF) method and laboratory-developed phase unwrapping algorithm. Authors found that it has no severe distortion due to the antenna inserted into the phantom. Finally, we can conclude that the suggested coaxial-slot antenna has an excellent local heating performance for both of tissue-mimic phantom and swine muscle, and it is compatible to 1.5T MRI scanner.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.24 no.6
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• pp.147-153
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• 2010

When small scale wind and solar power generation systems are connected to conventional power distribution system. It is worried that it can affect the quality of electricity such as voltage variation, power factor, frequency, harmonics and flicker. Therefore, in this research, in order to investigate the effects of wind power generation equipments(AC link method) on electrical power quality, when they are connected to distribution system. Power quality analyzer was installed respectively at the front side of power conversion system of conventional wind generation system and secondary side of consumer's power supply at distribution line. Measurements on power quality were performed and the effects of the wind generation system on distribution system were analyzed when it was and was not operated. The results show that 0.34[%] increase on voltage variation, 0.145 increase on current crest factor and 0.6[$^{\circ}$] deviation on phase difference when the power generation system was operated.