• Journal of Korean Society of Water and Wastewater
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• v.11 no.4
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• pp.63-70
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• 1997

The objectives of this study were to find out the optimum treatment conditions for removing nitrogen in a synthetic wastewater by using microorganisms immobilized with PVA-Freezing method. The samples used as influents to the laboratory scale treatment units were a synthetic wastewater. The experiments in this study were mainly directed to collect the data of nitrogen and organic matter removal efficiencies for the different hydraulic and internal recycle rates conditions, temperature and influent C/N ratios. The removal efficiencies of nitrogen and organic matters were investigated for the operating conditions of HRT 2~12hours, internal recycle rates 50~400%, temperatures $15{\sim}30^{\circ}C$ and C/N ratios 2.5~7.5. The adequate internal recycle rate for removing T-N and $BOD_5$ in the synthetic wastewater was found to be about 300% at the temperature of $30^{\circ}C$ when the ratio of carbon contents to the nitrogen (C/N) in the influent was around 5.5. Under these conditions, the final effluent concentrations of T-N and $BOD_5$ were 8.7 and 8.4 mg/l, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.37-71
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• 2021

Rapeseed is a typical winter crop, and its freezing stress tolerance is a major feature for winter survival. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of rapeseed under freezing stress conditions. This study investigates the physical and transcriptome changes of two rapeseed lines, 'J8634-B-30' and 'EMS26', under cold acclimation and freezing temperature treatments. The proline content of 'J8634-B-30' at 5 ℃ increased 8.7-fold compared to that before treatment, and there was no significant change in that of 'EMS26' RNA-sequencing analysis revealed 5,083 differentially expressed genes (DEGs) of 'J8634-B-30' under cold acclimation condition. Among the genes, 2,784 (54.8%) were up-regulated and 2,299 (45.2%) were down-regulated. The DEGs of 'EMS26' under cold acclimation condition were 5,831 genes, and contained 2,199 up-regulated genes (37.7%) and 3,632 down-regulated genes (62.3%). Among them, only DEGs annotated in the cold response-related signaling pathways were selected, and their expression in the two rapeseed lines was compared. Comparative DEGs analysis indicated that cold response related signaling pathways are proline metabolism and ABA (Abscisic acid) signaling. And ICE (Inducer of CBF expression) - CBF (C-repeat-binding factor) - COR (Cold-regulated) signaling were the significantly differentially expressed transcripts in the two rapeseed lines. The major induced transcripts of 'J8634-B-30' induced P5CS (Δ'-pyrroline-5-carboxylate synthetase), which is related to proline biosynthesis, PYL (pyrabactin resistance-like protein, ABA receptor) and COR413 (cold-regulated 413 plasma membrane 1). In conclusion, these result provide a foundation for understanding the mechanisms of freezing stress tolerance in rapeseeds. Further functional studies should be performed on the freezing stress-related genes identified in this study, which can contribute to the transgenic and molecular breeding for freezing stress tolerance in rapeseed.

• Journal of the Korean Geotechnical Society
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• v.27 no.9
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• pp.67-76
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• 2011

Bearing capacity of pile foundations in cold region is dominated by adfreeze bond strength between surrounding soil and pile perimeter. Adfreeze bond strength is considered to be the most important design parameter for foundations in cold region. Many studies in last 50 years have been conducted to analyze characteristics of adfreeze bond strength. However, most studies have been performed under constant temperature and normal stress conditions in order to analyze affecting factors like soil type, pile material, loading speed, etc. In this study, both freezing temperature and normal stress acting on pile surface were considered to be primary factors affecting adfreeze bond strength, while other factors such as soil type, pile material and loading speed were predefined. Direct shear box was used to measure adfreeze bond strength between Joomoonjin sand and aluminium because it is easy to work for various roughness. Test was performed with temperatures of > $0^{\circ}C$, $-1^{\circ}C$, $-2^{\circ}C$, $-5^{\circ}C$, and $-10^{\circ}C$ and vertical confining pressures of 1atm, 2atm, and 3atm. Based on the test results, the effects of temperature and vertical stress on adfreeze bond strength were analyzed. The test results showed that adfreeze bond strength increases with decreased temperature and increased vertical stress. It was also noted that two types of distinct sections exist, owing to the rate of increase of adfreeze bond strength along the change of freezing temperature: 1)rapidly increasing section and 2)gradually decreasing section. In addition, the results showed that a main factor affecting adfreeze bond strength switches from friction angle to adhesion as freezing temperature decreases.

• Korean Journal of Animal Reproduction
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• v.22 no.2
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• pp.187-194
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• 1998

In order to determine suitable conditions for rapid freezing of porcine embryos, the kind and concentration of cryoprotectants, sucrose concentrations, equilibration time and thawing temperature in freezing medium were examined in relation to the survival of frozen-thawed oocyte and embryos. The results obtained are as follows : 1. The suitable concentrations of cryoprotoctant in the freezing medium which consisted of TCM-199+20% FCS were 1.5M for glycerol, 2.0M for DMSO, 2.5M for ethylene glycol, and 2.0M for propanediol. The sucrose concentration of 0.25M in the medium was found to optimal because the survival rate was markedly higher at this concentration when compared to the others. The survival rate was relatively high when the frozen embryos were thawed at 30$^{\circ}C$ in the freezing medium containing 2.5M cryoprotectants. The equilibration periods of 2.0 and 5.0 minutes revealed the higher survival in the media containing 1.5 or 2.1M glycerol when compared to 10 and 15 minutes. 2. The fertilization rates of frozen-thawed follicular oocytes which matured in vitro for 1, 12, 24 and 48 hours were 6.7~26.7% depending on the maturation time, and the rates were relatively high for those matured for a short period of time. The survival rates of frozen-thawed oocytes which matured in vitro for certain periods and fertilized were 10.0~30.0% depending on the maturation time.

• Journal of the Korean GEO-environmental Society
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• v.17 no.6
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• pp.23-31
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• 2016

Nowadays abnormal coldness happens frequently in Korea and frost heaving causes unexpected ground deformation which results in severe problems for structures such as roadway, railroad and cutoff slope. 'Frost heave' as one of the primary phenomenon is considered to be an important factor together with 'adfreeze bond-strength' and 'creep deformation' for structural design process in permafrost area. Therefore, the fundamental study for frost heave has to be preceded for design of geo-structures in cold region. While various experimental apparatuses have been developed, there still exist a certain level of limitation to evaluate the frost-heave characteristics as design parameters. There are no standard testing method and criteria for analyzing frost heaving in Korea because temperature controlled testing apparatuses including a freezing chamber are expensive. In this paper, a new standard freezing and thawing testing apparatus is introduced, which simulates various freezing and thawing conditions in a soil specimen by using a temperature controlled triaxial cell. Frost heaving tests were performed to assess the new testing apparatus and experimental procedure to evaluate frost heaving for soils is proposed.

• Journal of the Korean Geotechnical Society
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• v.39 no.12
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• pp.115-125
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• 2023

In this study, we evaluated the behavior of frozen soil using an electrical resistivity survey method-a nondestructive technique-and examined its characteristics through field experiments. Frozen soil was artificially prepared by injecting fluid to accelerate the freezing process, and naturally frozen soil was selected in a nearby area for comparison. A dynamic cone penetration test (DCPT) was performed to compare the reliability of the electrical resistivity survey, and time-domain reflectometry surveys were performed to assess the moisture content of the ground. Field experiments were conducted in February-when the atmosphere temperature was below freezing-and May-when the temperature was above freezing. This temperature-compensated method was used to determine reliability because the behavior of frozen soil depends on the underlying temperature. In the resistivity survey method, a section of high electrical resistivity was observed under freezing conditions due to the frozen water and converted into porosity. The converted porosity was compared with the porosity inferred from the DCPT, and the results showed that the measured electrical resistivity was valid.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.10
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• pp.1550-1557
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• 2015

This study was conducted to evaluate the improvement effects of bitter taste of doraji (Platycodon grandiflorum) with different freezing temperature conditions ($-5^{\circ}C$, $-10^{\circ}C$, $-20^{\circ}C$, and $-70^{\circ}C$). After freezing for 4 h, frozen doraji was thawed and dehydrated. Doraji frozen at $-20^{\circ}C$ showed higher contents of crude fat, crude protein, crude ash, crude fiber, and nitrogen free extract. Moisture, crude saponin, and total polyphenol contents of doraji frozen at $-5^{\circ}C$ were higher than in other samples. Weight loss of doraji was significantly different as freezing temperature decreased and the process repeated. Doraji frozen at $-5^{\circ}C$ showed higher contents of saponin and phenol than the control. Hardness of doraji increased as freezing temperature decreased. According to the preference test, Doraji frozen at $-5^{\circ}C$ showed higher preference and less bitter taste intensity than other samples. These results mean that doraji frozen at $-5^{\circ}C$ with less bitter taste could be utilized widely as a food material.

• Korean journal of food and cookery science
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• v.13 no.1
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• pp.40-46
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• 1997

This study was conducted in order to determine the effect of preparing conditions(refrigerating and freezing of dough, dough size, frying temperature, kneading degree and ingredient amount) on the dehydration, absorbed oil content and sensory characteristics of Yackwa. There were no significant differences on the dehydration, absorbed oil content and sensory characteristics between the control Yackwa and those prepared by refrigerating and freezing of dough. In the dough size (large, medium, small), dehydration and absorbed oil content was increased as the size became smaller. It was shown that overall acceptability was high in medium size Yackwa. There were no significant differences on the dehydration of Yackwa under various frying temperature and kneading degree. On the other hand, absorbed oil content of Yackwa was decreased as the frying temperature became higher and kneading degree increased. Dehydration of Yackwa was increased as the amount of sesame oil, honey and sozu increased. There were no significant differences on the absorbed oil content of Yackwa as the amount of sesame oil increased. The absorbed oil content was increased as the amount of honey increased, whereas the absorbed oil content decreased as the amount of sozu increased. Shininess, softness, brittleness and oily taste was increased as the absorbed oil content increased in sensory evaluation of Yackwa prepared by different frying temperature, kneading degree and ingredients amount. It was shown that above preparing conditions affected the absorbed oil content and sensory characteristics of Yackwa.

• Magazine of the Korean Society of Agricultural Engineers
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• v.26 no.3
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• pp.46-58
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• 1984

This study was carried out to investigate the compressive strength and the other effects varying to seasons and curing days on the wet curing conditions of the plain concrete. The results obtained are summarized as follows; 1. The longer the wet curing days and the higher the temperature, the greater the compressive strength was expected. 2.。$_2$8, compressive strength of concrete at 28 days under the dry curing conditions showed a range in 64-76% of that under the wet curing conditions. 3. The seasonal variations in the compressive strength under the wet curing showed in order of summer>spring=autumn>winter, and that under the dry curing were in order of spring ≒autumn> summer> winter. 4. In order to obtain 90% of the design compressive strength, 7 days in spring or autumn and 2 weeks of the wet curing in summer were required. 5. The compressive strength of concrete under the wet curing by using wet straw bag cover was almost the same as that of water curing method. 6. Under the wet curing conditions, the higher the temperature, the greater the effect of the curing of concrete was obtained, however, the compressive strength of concrete was decreased under relatively higher (over 15$^{\circ}$ C) and lower temperature (below 4$^{\circ}$C). 7. Freezing damage was occured when temperature was below 0$^{\circ}$ C and humidity was relatively high. 8. A considerable differnce between estimation of $^{\circ}$$_2$8 from $^{\circ}$7 and measured one was appeared in case of the dry curing conditions. Oregon formula was appeared to be acceptable under the wet curing conditions. 9. In relationship between $^{\circ}$$_2$8 and $^{\circ}$7~, $^{\circ}$28=1. 52 $^{\circ}$7 under the wet curing conditions except winter season, and $^{\circ}$$_2$8 =(1.39-1, 48)$^{\circ}$7 under the dry curing conditions were shown.

• Journal of the Korean Geotechnical Society
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• v.39 no.5
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• pp.51-63
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• 2023

Numerical analysis was conducted to determine the effect of soil behavior by thawing and freezing of seasonal frozen soil on pile foundations. The analysis was performed using the finite element method (FEM) to simulate soil-pile interaction based on the atmosphere temperature change. Thermomechanical coupled modeling using FEM was applied with the temperature-dependent nonlinear properties of the frozen soil. The analysis model cases were applied to the MCR and HDP models to simulate the elastoplastic behavior of soil. The numerical analysis results were analyzed and compared with various conditions having different length and width sizes of the pile. The results of the numerical analysis showed t hat t he HDP model was relat ively passive, and t he aspect and magnit ude of t he bearing capacit y and displacement of the pile head were similar depending on the length and width of the pile conditions. The vertical displacement of the pile head by thawing and freezing of the ground showed a large variation in displacement for shorter length conditions. In the MCR model, the vertical displacement appeared in the maximum thaw settlement and frost heaving of 0.0387 and 0.0277 m, respectively. In the HDP model, the vertical displacement appeared in the maximum thaw settlement and frost heaving of 0.0367 and 0.0264 m, respectively. The results of the pile bearing capacity for the two elastoplastic models showed a larger difference in the width condition than the length condition of the pile, with a maximum of about 14.7% for the width L condition, a maximum of about 5.4% for M condition, and a maximum of about 5.3% for S condition. The significance of the effect on the displacement of the pile head and the bearing capacity depended on the pile-soil contact area, and the difference depended on the presence or absence of an active layer in the soil and its thickness.