• International Journal of Highway Engineering
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• v.15 no.1
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• pp.1-9
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• 2013

PURPOSES: The purpose of this study is to evaluate physical properties, durability, fatigue resistance, and long-term performance of poly-urethane concrete (PU) which can be possible application of thin layer on long-span orthotropic steel bridge and to check structural stability of bridge structure. METHODS : Various tests of physical properties, such as flexural strength, tensile strength, bond strength and coefficient of thermal expansion tests were conducted for physical property evaluation using two types of poly urethane concrete which have different curing time. Freezing and thawing test, accelerated weathering test and chloride ion penetration test were performed to evaluate the effect of exposed to marine environment. Beam fatigue test and small scale accelerated pavement test were performed to assess the resistance of PU against fatigue damage and long-term performance. Structural analysis were conducted to figure out structural stability of bridge structure and thin bridge deck pavement system. RESULTS: The property tests results showed that similar results were observed overall however the flexural strength of PUa was higher than those of PUb. It was also found that PU materials showed durability at marine environment. Beam fatigue test results showed that the resistances of the PUa against fatigue damage were two times higher than those of the PUb. It was found form small scale accelerated pavement test to evaluate long-term performance that there is no distress observed after 800,000 load applications. Structural analysis to figure out structural stability of bridge structure and thin bridge deck pavement system indicated that bridge structures were needed to increase thickness of steel deck plate or to improve longitudinal rib shape. CONCLUSIONS: It has been known that the use of PU can be positively considered to thin layer on long-span orthotropic steel bridge in terms of properties considered marine environment, resistance of fatigue damage and long-term performance.

• Journal of The Geomorphological Association of Korea
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• v.19 no.1
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• pp.83-97
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• 2012

Slopes of Seoseokdea~ Jangbuljae, at Mt. Moodeung, appears repeatedly the cliffs are mostly greater than $70^{\circ}$, and the planations maintain around $5^{\circ}$ in general. They studied the creation of development environments, cryoplanation are mainly facing the southwest. It is assumed that rock falling had been wostly occurred under a periglacial environment through jointing due to repeated freezing and thawing. Planation at the bottom of Columnar joint are described structural benches. Movement precesses of planation matrix are solifluction or jelifluction. The result of age determination of the slopes in Jangbuljae tells that the upper part of Ibseokdae has been remaining exposed to the land surface from 110,000 year ago. The blocks in the bottom part has been exposed to the surface around 10,000 year ago while Jangbuljae has been exposed to the surface about 50,000 year ago. It was assumed that they moved to the current location since being separated from columnar joint after exposure to the surface.

• Geomechanics and Engineering
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• v.25 no.1
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• pp.41-48
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• 2021

In cold regions, the integrity of the infrastructures built on weak soils can be extensively damaged by weathering actions due to the cyclic freezing and thawing. This damage can be mitigated by exploiting soil stabilization techniques. Generally, ordinary Portland cement (OPC) is the most commonly used binding material for investigating the chemo-hydromechanical behavior. However, due to the environmental issue of OPC producing a significant amount of carbon dioxide emission, calcium sulfoaluminate (CSA) cement can be used as one of the eco-sustainable alternatives. Although recently several studies have examined the strength development of CSA treated sand, no research has been concerned about CSA cement-stabilized sand affected by cyclic freeze and thaw. This study aims to conduct a comprehensive laboratory work to assess the effect of the cyclic freeze-thaw action on strength and durability of CSA cement-treated sand. For this purpose, unconfined compressive strength (UCS) and ultrasonic pulse velocity (UPV) tests were performed on the stabilized soil specimens cured for 7 and 14 days which are subjected to 0, 1, 3, 5, and 7 freeze-thaw cycles. The test results show that the strength and durability index of the samples decrease with the increase of the freeze-thaw cycles. The loss of the strength and durability considerably decreases for all soil samples subjected to the freeze-thaw cycles. Overall, the use of CSA as a stabilizer for sandy soils would be an eco-friendly option to achieve sufficient strength and durability against the freeze-thaw action in cold regions.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.2
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• pp.138-144
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• 2019

To analyze the present state of buildings suspended in Gangwon and estimate the compressive strength by visual inspection and Schmidt hammer method in order to analyze durability etc.. In this study, we analyzed the problems that existed in the location where construction is suspended and efficient management method. Expected construction restoration of construction will be restarted, and important parts of the construction site should be given the best protection measures so that the quality can be maintained thoroughly. The construction of the suspended construction is exposed to the freezing and thawing damage over time. Therefore, it is necessary to take measures such as maintenance, and take protective measures by establishing a plan to improve the durability of buildings that are under construction.

• Journal of the Korea Institute of Building Construction
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• v.19 no.4
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• pp.307-312
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• 2019

Sludge generated in the production of ready-mixed concrete is classified as waste and processed at a high cost. In particular, small and medium-sized ready-mix manufacturer are burdened with such costs, and some companies are illegally processing them. Therefore, the purpose of this study is to suggest a method for recycled remicon sludge as a concrete block composition. When the remicon sludge is simply dried, the residual chemical admixture and ettringitee contained in the sludge are present, so that the compressive strength of the concrete block and the compressive strength after freezing and thawing are largely deteriorated to meet the quality standards of the concrete shore and retaining wall block It was not possible to do. As a method for satisfying the physical performance, it was found that the remicon sludge was calcined at a high temperature of about $900^{\circ}C$. to decompose ettringite and residual chemical admixture and then used it.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.299-306
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• 2021

Vitrification methods are commonly used for mammalian reproduction through the long-term storage of blastocyst produced in vitro. However, the survival and quality of embryos following vitrification are significantly low compared with blastocyst from in vitro production (IVP). This study evaluates that the survival of frozen-thawed bovine embryos was relevant to mitochondrial superoxide derived mitochondrial activity. Here we present supplementation of the cryopreservation medium with Mito-TEMPO (0.1 µM) induced a significant (p < 0.001; non-treated group: 56.8 ± 8.7%, reexpanded at 24 h vs Mito-TEMPO treated group: 77.5 ± 8.9%, re-expanded at 24 h) improvement in survival rate of cryopreserved-thawed bovine blastocyst. To confirm the quality of vitrified blastocyst after thawing, DNA fragmentation of survived embryos was examined by TUNEL assay. As a result, TUNEL positive cells rates of frozen-thawed embryos were lower in the Mito-TEMPO treated group (4.2 ± 1.4%) than the non-treated group (7.1 ± 3.5%). In addition, we investigated the intracellular ROS and mitochondrial specific superoxide production using DCF-DA and Mito-SOX staining in survived bovine embryos following vitrification depending on Mito-TEMPO treatment. As expected, intracellular ROS levels and superoxide production of vitrified blastocysts after cryopreservation were significantly reduced (p < 0.05) according to Mito-TEMPO supplement in freezing medium. Also, mitochondrial activity measured by MitoTracker Orange staining increased in the frozen-thawed embryos with Mito-TEMPO compared with non-treated group. These results indicate that the treatment of Mito-TEMPO during cryopreservation might induce reduction in DNA fragmentation and apoptosis-related ROS production, consequently increasing mitochondrial activation for developmental capacity of frozen-thawed embryos.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.2
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• pp.113-120
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• 2022

Sperm cryopreservation is a fundamental process for the long-term conservation of livestock genetic resources. Yet, the packaging method has been shown, among other factors, to affect the frozen-thawed (FT) sperm quality. This study aimed to develop a new mini-straw for sperm cryopreservation. In addition, the kinematic patterns, viability, acrosome integrity, and mitochondrial membrane potential (MMP) of boar spermatozoa frozen in the developed 0.25 mL straw, 0.25 mL (minitube, Germany), or 0.5 mL (IMV technologies, France) straws were assessed. Post-thaw kinematic parameters were not different (experiment 1: total motility (33.89%, 32.42%), progressive motility (19.13%, 19.09%), curvilinear velocity (42.32, 42.86), and average path velocity (33.40, 33.62) for minitube and the developed straws, respectively. Further, the viability (38.56%, 34.03%), acrosome integrity (53.38%, 48.88%), MMP (42.32%, 36.71%) of spermatozoa frozen using both straw were not differ statistically (p > 0.05). In experiment two, the quality parameters for semen frozen in the developed straw were compared with the 0.5 mL IMV straw. The total motility (41.26%, 39.1%), progressive motility (24.62%, 23.25%), curvilinear velocity (46.44, 48.25), and average path velocity (37.98, 39.12), respectively, for IMV and the developed straw, did not differ statistically. Additionally, there was no significant difference in the viability (39.60%, 33.17%), acrosome integrity (46.23%, 43.23%), and MMP (39.66, 32.51) for IMV and the developed straw, respectively. These results validate the safety and efficiency of the developed straw and highlight its great potential for clinical application. Moreover, both 0.25 mL and 0.5 mL straws fit the present protocol for cryopreservation of boar spermatozoa.

• Journal of the Korean Recycled Construction Resources Institute
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• v.9 no.4
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• pp.413-418
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• 2021

In this study, as a basic study to use recycled limestone powder as a secondary product mixture for concrete, it was found that the compressive and flexural strengths were equal to or slightly improved compared to Plain up to 10% and 20% of the RLP mixing ratio, but the strength was rather decreased at 30% mixing. As a result of the heat of hydration experiment, as the RLP mixing rate increased, the heat of hydration decreased, and the elapsed time of the maximum heat was also delayed. As a result of the drying shrinkage test, as the fine powder RLP filled the internal pores of the cement mortar, the drying shrinkage decreased as the mixing rate increased. The compressive strength, water absorption rate, and compressive strength after freezing and thawing of the concrete block mixed with RLP 20% all satisfied the group standard criteria of the Korea Concrete Industry Cooperative Federation, confirming the possibility of use as a mixed material.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.4
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• pp.386-394
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• 2022

In this study, the mechanical properties and frost resistance of concrete with steel and nylon fibers were experimentally investigated. Both of OPC concrete with 100 % ordinary portland cement and SGC concrete replaced with 50 % GGBFS were manufactured to evaluate effects of fibers to the performance of concrete. Compressive and split tensile strength, ultrasonic pulse velocity and surface electric resistivity measurements of concrete were carried out at a predetermined interval. In addition, the freezing & thawing resistance of concrete in accordance with ASTM C666 standard was also examined. As a result, it is seemed that the effect of fibers was remarkable to improve the mechanical properties and frost resistance of concrete, especially for the concrete incorporating steel fiber.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.2
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• pp.130-135
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• 2022

Epididymal sperm cryopreservation provides a potential method for preserving genetic material from males of endangered species. This pilot study was conducted to develop a freezing method for tiger epididymal sperm. We evaluated post-thaw sperm condition using testes with intact epididymides obtained from a Siberian tiger (Panthera tigris altaica) after castration. The epididymis was chopped in Tyrode's albumin-lactate-pyruvate 1x and incubated at 5% CO₂, 95% air for 10 min. The Percoll separation density gradient method was used for selective recovery of motile spermatozoa after sperm collection using a cell strainer. The spermatozoa were diluted with modified Norwegian extender supplemented with 20 mM trehalose (extender 1) and subsequent extender 2 (extender 1 with 10% glycerol) and frozen using LN₂ vapor. After thawing at 37℃ for 25 s, Isolate^® solution was used for more effective recovery of live sperm. Sperm motility (computerized assisted sperm analysis, CASA), viability (SYBR-14 and Propidium Iodide) and acrosome integrity (Pisum sativum agglutinin with FITC) were evaluated. The motility of tiger epididymal spermatozoa was 40.1 ± 2.0%, and progressively motile sperm comprised 32.7 ± 2.3%. Viability was 56.3 ± 1.6% and acrosome integrity was 62.3 ± 4.4%. Cryopreservation of tiger epididymal sperm using a modified Norwegian extender and density gradient method could be effective to obtain functional spermatozoa for future assisted reproductive practices in endangered species.