• Journal of Embryo Transfer
• /
• v.27 no.4
• /
• pp.259-264
• /
• 2012

Solution of glycerol, ethylene glycol, sucrose, dextrose (GESD) and cryotop methods were carried out to investigate the survivability on vitrification of embryos. Embryos cultured in vitro were vitrified by GESD of 10 or 8 step and cryotop methods of 6 step, from cryopreservation step to frozen-thawed and culture step. Survival rate and ICM, TE cells of embryos were investigated after frozen-thawed 24 h. As a results, cryotop method was significantly (p<0.05) higher ($85.76{\pm}5.3$ vs. $66.71{\pm}2.4$, $44.80{\pm}2.1%$) than GESD 10 or 8 step methods on survivability. Also, In ICM cell number, cryotop method was significantly (p<0.05) higher to $45.67{\pm}4.7$ cells than GESD 8 step method. TE cell number was significantly (p<0.05) highest to $111.00{\pm}11.0$ cells in cryotop method. On the other hand, survival rate, TE and total cell number were all the significantly (p<0.05) high, except ICM in GESD 10 step method between GESD 10 step method and GESD 8 step method. In conclusion cryotop method was to be most effective, but it is considered necessary to study vitrification method for step-by-step freezing and thawing process.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.18 no.4
• /
• pp.92-98
• /
• 2014

Spacer is a construction material for maintaining cover depth and steel installation, however several problems like staining, leakage, and cracking are currently issued due to performance degradation and unsatisfactory dimensional stability of spacer. Plastic composite is widely used for prevention of brittle failure in cement based material, which yields improvement of crack resistance and ductile failure. This study is for development and applicability evaluation of high strength spacer with slag cement for environmental load reduction and plastic composite like polypropylene fiber, nylon fiber, and glass fiber. For this work, unit weight of 4 different plastic fibers are evaluated through preliminary tests. Physical tests including compressive, flexural, and tensile strength and durability tests including absorption, permeability, length change, crack resistance, carbonation, and freezing and thawing are performed. Through various tests, optimum plastic fiber is selected and manufacturing system for high strength spacer with the selected fiber is developed. Dimensional stability of the developed spacer is evaluated through field applicability evaluation.

• Journal of the Korean Geotechnical Society
• /
• v.30 no.8
• /
• pp.39-47
• /
• 2014

Frost heaving and thawing settlement cause unexpected stress around buried pipelines, which results in deformation and permanent demage. A large scale laboratory test has been performed to observe deformation, stress, and temperature of a buried pipe during atmospheric temperature changes. From the experimental results, the stress concentrated around the buried pipe is inevitable and deformation is caused by the frost heaving. Even though backfill materials are sandy soils which are normally assumed to be non frost susceptible, it is revealed that frost demage can happen due to drainage condition, the level of ground water table, and water content.

• Proceedings of the Korean Institute of Resources Recycling Conference
• /
• 2003.10a
• /
• pp.134-138
• /
• 2003

The purpose of this paper is to recycle the fly ash to the valuable resources and settle environment problems which was caused by the fly ash produced from the thermal power plant. Making the fly ash-cement matrix reused fly ash in large quantities, we looked into minutely the physical properties - the elastic modulus, the compressive strength - to increase the usefulness as the building materials for the structure widely. In this paper, the variables are the water-binder(39, 42, 45%), the fine aggregate ratio(37, 41, 45%). Because the fracture energy is influenced by the strength, it is showed to decrease with the increase of W/B and S/a. Besides, we will be able to know that basic properties of the fly ash-cement matrix are similar to that of concrete. But, it is needed to carry out durability experiment on the drying shrinkage, creep, freezing and thawing test to use structural materials.

• Korean Journal of Agricultural Science
• /
• v.45 no.2
• /
• pp.177-184
• /
• 2018

The frost heave process from repeated freezing and thawing actions in winter on forest road cut-slopes is important for forest road maintenance and management. This study investigated the damages of the forest heave process on forest road cut-slopes by measuring the changes in the road-cut surface elevation and sediment production and by conducting vegetation surveys which were aimed at providing information for forest road maintenance plans. The temperature and humidity differences were determined between the north and south cut-slopes. T-test showed that the north slope had a lower temperature and humidity than that of the south slope. Field observations also confirmed frozen soils on the north slopes, indicating that the north slopes are susceptible to frost heave. Sediment was converted to dry weight per unit area ($g/m^2$). T-test showed that the north slope produced more sediment than that of the south slope. The study confirmed that more frost heave occurred on the north cut-slopes than on the south cut-slopes. Vegetation surveys were conducted on five cut-slope plots. Considering the dominant species found above the cut-slopes, vegetations in all the plots are expected to succeed to pine and oak in the future. The dominant species appearing on the cut-slopes of the study area were exotic species because the elapsed time of the site was only 2 - 4 years.

• KIISE Transactions on Computing Practices
• /
• v.22 no.1
• /
• pp.8-19
• /
• 2016

Potholes are caused by the presence of water in the underlying soil structure, which weakens the road pavement by expansion and contraction of water at freezing and thawing temperatures. Recently, automatic pothole detection systems have been studied, such as vibration-based methods and laser scanning methods. However, the vibration-based methods have low detection accuracy and limited detection area. Moreover, the costs for laser scanning-based methods are significantly high. Thus, in this paper, we propose a new pothole detection system using a commercial black-box camera. Normally, the computing power of a commercial black-box camera is limited. Thus, the pothole detection algorithm should be designed to work with the embedded computing environment of a black-box camera. The designed pothole detection algorithm has been tested by implementing in a black-box camera. The experimental results are analyzed with specific evaluation metrics, such as sensitivity and precision. Our studies confirm that the proposed pothole detection system can be utilized to gather pothole information in real-time.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.44 no.4
• /
• pp.139-148
• /
• 2002

Pilot study was performed to examine the feasibility of the pond system for further polishing of treatment wetland effluent from December 2000 to June 2001. The wetland system used for the experiment was highly effective to treat the sewage during the growing season, but it was less effective and its effluent was still high to discharge to the receiving water body. Therefore, the wetland effluent may need further treatment to prevent water quality degradation. Pond system could be used to hold and further polish the wetland effluent during the winter season and ots feasibility was evaluated in this study. Additional water quality improvement was apparent in the pond system during winter season, and the pond effluent could be good enough to meet the effluent water quality standards if it is properly managed. Timing of the pond effluent discharge appears to be critical for pond system management because it is a closed system and whole water quality constituents are affected by physical, chemical, and biological pond environments. Once algae started to grow in mid-April, constituents in the pond water column interact each other actively and its control becomes more complicated. Therefore, upper layer of the pond water column which is clearer than the lower layer my need be discharged in March right after ice cover melted. In the experiment, water quality of the upper water column was markedly clear in March than ant other times probably because of freezing-thawing effect. The remaining lower water column could be further treated by natural purification as temperature goes up or diluted with better quality of wetland effluent for appropriate water uses. This study demonstrated the feasibility of pond system for subsequent management of wetland effluent during the winter season, however, more study is needed for field application.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.34 no.6
• /
• pp.1743-1751
• /
• 2014

It is well known that latex modification can lead to enhancement compressive and flexural performance, tensile bond strength, chloride penetration resistance and freezing-thawing resistance for concrete. For these, many studies for LMC pavement have been widely conducted more than those for conventional pavement concrete. However, due to several problems such as noise and construction cost, new approaches for LMC have been executed to improve the performance of LMC. Recently, Remicon LMC was developed in order to solve the problems of LMC. In this study, the durability of Remcion LMC was compared with latex and mineral additive mixing ratio. From the results, when latex and mineral additive were mixed in Remicon LMC, compressive, flexural and bond strength were satisfied with Korea Highway Construction Guide Specification. And, it showed that the qualitative effect of latex and mineral additive mixing ratio on the durability of Remicon LMC was investigated experimentally. Also, the latex mixed in Remicon LMC must be at least 8%, in order to ensure the durability equivalent or higher than conventional LMC.

• Magazine of the Korea Concrete Institute
• /
• v.4 no.1
• /
• pp.89-96
• /
• 1992

It is generally known that the frost-resistance of concrete is much affected by the air content in concrete and by the air void system or air distribution. And also the frost-resistance is believed to vary with the stre¬ngth of concrete. This article is prepared to describe, based on experiment, the effect of the air content and the air void system, particularly the effect of the spacing factor, on the freeze-thaw resistance of the high strength conc¬rete. For this purpose, I first worked on Non-AE concrete to make its compressive strength set about 400 to 500 kg/em'. However, the freeze-thaw test on the Non-AE concrete resulted in low durability factor, I.e., 10-2~0%. Thus to enhance the durability, another supplementary step was needed. I used AE admixture. which enhanced durability by changing the air content from 2% to 12%. The frost-thaw test was then performed 500 cycles on the 20 kind of concrete mixtures which differ in unit cement content and in water-cement ratio. Keywords : frost -resistance, air content, air void system, air distribution, spacing factor, freeze-thaw test, dur ability factor. capillary cavity, Linear Traverse Method.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.6 no.1
• /
• pp.120-127
• /
• 2011

Recently, there are problems due to the exhaustion of natural aggregate resources, and strict restrictions. In this study, the possibility of using Water Granulated Ferro-Nickel slag as a substitutive material of fine aggregate is determined from the properties of mechanical and durability for the concrete that is made with Water Granulated Ferro-Nickel slag. According to the test results, when the mixing rate of Water Granulated Ferro-Nickel Slag aggregates concrete is adjusted, up to 50% of its aggregates by mixing rate can be mixed with general aggregates. The optimum mix ratio is considered to be 40%. The freezing and thawing resistance of Water Granulated Ferro-Nickel Slag aggregates concrete is identical to that of general aggregates concrete, while the carbonation resistance is found to be same as or lower than that of general aggregates concretes.