• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.3
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• pp.176-178
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• 2000

The toxicity of muscle in pufferfish, Tikifugu vermicularis radiatus is known to be weak. However, no food poisoning has reported in this species although market consumption has been relatively high in Korea, especially on western coastal area. It presumes that there could be different toxicity in fish muscle of fresh and frozen specimens. In this regard, the frequency of toxic fish, the toxicity level in fresh and frozen fish as well as highest toxicity ann average toxicity were investigated. From the results, weak toxicity was detected in frozen fish muscle whereas no toxicity was measured in fresh fish. According to this, it is suggested that the toxicity of frozen and fresh fish must be tested simultaneously when tonicity of pufferfish is investigated. The above results implicate that toxicity of pufferfish may be due to the phsyical treatment such as freezing or thawing process.

• IMMUNE NETWORK
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• v.6 no.3
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• pp.145-153
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• 2006

Background: Tumor cell lysate has been considered as a preferential antigen source for the therapeutic dendritic cell pulsing. Our experiences with in vivo study with animal tumor model indicate the tumor cell lysate dependent differential effect of DC therapy. Our previous data show that MC38 lysate pulsed-DC induced stronger ag-specific immunity than CT26 lysate pulsed-DC in vitro. In this study we tried to reveal the mechanism for differential induction of ag-specific immunity of different colon cancer cell lysate pulsed-DCs. Methods: MC38 and CT26 cell lines were prepared as lysate by freezing-thawing procedure. Tumor cell antigenicity was confirmed by detecting the surface expression of MHC I/II & B7.1/2 molecules. IL-10, IL-12 and TGF-beta in the tumor cell lysate were detected by ELISA and the presence of heat shock proteins were analysed by western blotting. Results: The secretion of IL-10, a immune-inhibitory cytokine was about 470% higher in CT26 lysate than in MC38. Hsp 70 was detected only in the MC38 lysate but not in the CT26. On the other hand, Hsp 60 and 90 expression were not different in two colon cancer cell lysates. Conclusion: In two different colon cancer cell lysate, immune inhibitory IL-10 (higher in CT26) and Hsp70 (MC38 superiority) were differentially expressed. These data indicate that higher agspecific immunity induction by MC38 lysate pulsed-DC may due to the expression of hsp70 and lower secretion of IL-10, a immune-inhibitory cytokine than CT26 lysate. The significance of other cytokine and the surface marker expression will be discussed.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.3
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• pp.140-148
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• 2016

This study examined the durability of face-slab concrete in Concrete-Faced Rock-filled Dams(CFRDs). The durability of face-slab concrete can be improved by optimizing the amount of fly ash in the cement mixture. Durability tests including plastic shrinkage, permeability, abrasion resistance, and repeated freezing and thawing were done on face-slab concrete specimens with different amounts of fly ash and blended PVA(Poly Vinyl Alcohol) fibre. When the effect of the fly ash content on concrete durability was evaluated, the results showed that a 15% fly ash content and 0.1% blended PVA fiber yielded the optimum durability level for concrete-faced rock-filled dams.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.4
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• pp.124-131
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• 2017

The purpose of this study is to evaluate the basic performance of cement mortar for repair using alumina cement and nitrite can be cured in low temperature environment. For this purpose, the repair mortar used in the domestic construction site was selected and the experimental evaluation was carried out by adjusting the mixing amount by substituting alumina cement and nitrite for the blending ratio. The experimental test results confirmed that alumina cement and nitrite were replaced with the repair mortar, the initial strength was improved. Also, the chemical resistance was improved, the shrinkage behavior was decreased, and the resistance to freezing and thawing was increased. As a result, applying alumina cement and nitrite at a ratio of 2:1 at 7.5%, the surface condition was maintained for 5 months or longer and it was judged to be excellent in practical use for external structures.

• Journal of the Korean Recycled Construction Resources Institute
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• v.2 no.1
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• pp.40-45
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• 2014

Although the accelerators are used at the early stage to control setting-time and strength of concrete when cold-weather concrete is utilized, no security of workability occurs because the early hydration makes them react rapidly. Therefore, the tablet used in previous study is applied in this study. In particular, because a small amount of fly-ash being replaced in cold weather concrete of domestic, fly-ash concrete incorporating the tablet is discussed in workability by elapsed time, early strength to ensure the development of adequate strength, and freezing-thawing resistance. As a result, both 0.5 and 1.0% tablets were found to be superior. Thus, it was verified in cold weather concrete incorporating fly-ash that workability can be secured, as well as the development of early strength to prevent early frost.

• Journal of the Korean association of regional geographers
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• v.8 no.4
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• pp.468-479
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• 2002

This study attempts to characterize Taebaek mountainous region as a natural unit, relating to morphoclimatic milieu and landform development patterns of the mountains of each lithologic type. In the case of granitic mountains of Nothern parts of this region, the slopes presents more or less steep or irregular profile because of abundant microforms of exposed bedrocks and blocks. The development of such landscape is essentially due to differential weathering, associated with difference of joint density. In the case of gneissic mountains, the slopes are well regularized straight steep ones, due not only to generalized superficial weathering but also to massmovement of weathered materials. In the Middle parts of this region, especially in Taegwallyong area, with frequent alternations both of freezing-thawing and of snow accumulation-melting, the roles played by cryo-nival processes proved to be important in weathering of granite as well as in morpho-pedogenesis. In this area, weathered mantle, developed by cryogenic activity under humid condition of nival environment, covered almost all over the slopes. Although Southern parts of this region consist of limestone, on the mountainous volume, distribution of Karst forms are limited while predominate none karst forms such as cockpit type peaks, V-shaped type valleys dissecting steep slopes covered with thin deposits in thickness containing rock debris.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.3
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• pp.13-24
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• 1993

The influence of the bubble structure and strength characteristics on the freeze-thaw resistance of high strength concrete is investigated by the laboratory experiment. The test conditions are formed in the manner that water is continueusly supplied externally and the specimens were received severe weather actions from ordinary to significantly low temperatures. The experiments are performed in two stages. In the first stage, the relation between the durability to frost action and the bubble structure is analyzed especially with respect to the water-cement ratio and the amount of air. The AE and non-AE concrete specimens made of ordinary portland cement are used in the test. In the second stage, the non-AE concrete specimens using vibratory compaction to improve the durability to frost action, and the high watertight specimens of rapid hardening portland cement to increase their initial strength are produced and tested. The degree of watertightness of the specimens is determined by measuring the permeability of the specimens and the bubble structure of the high watertight concrete is also estimated.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.4C
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• pp.139-147
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• 2012

In this study, various laboratory tests using bottom ash, which has similar engineering properties with sand, were conducted in order to solve the problem of clogging in granular compaction pile and to address sand supply and demand. In particular, testing was performed to help reduce clogging and minimize voids in a crushed stone compaction pile constructed in soft ground. Based on compaction tests and large diameter direct shear tests, an optimum mixing ratio was determined to be 80:20 (crushed stone to bottom ash) because an 80:20 mixing ratio showed the highest shear strength. Test results showed that as the bottom ash content increased above 20%, internal friction angle decreased. Another test method showed freezing and thawing had little effect when the replacement ratio was over 40%. Therefore, bottom ash mixed compaction piles in soft ground are most economical at a 40% replacement ratio.

• Korean Journal of Veterinary Research
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• v.41 no.1
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• pp.89-98
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• 2001

This study was conducted to detect the toxoplasma specific-DNA in circulating blood and organs collected from slaughtered pigs at slaughtering house and experimentally infected pigs with Toxoplasma gondii tachyzoites by polymerase chain reaction(PCR), and also PCR was applied to diagnose for acute phase of swine toxoplasmosis as a newly developed diagnostic test. The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with Tp parasite detection by mouse inoculation(MI). On the other hand, latex agglutination test(LAT) was conducted to detect the serum antibodies comparing with the detection of toxoplasma by PCR and MI. The results obtained were summarized as follows. PCR was able to determine at the lowest level of $10^0/ml$ T. gondii in blood samples which were blended with a serial diluted T gondii in vitro. On the other hand, $10^2/5g$ of T gondii could detect from a variety of tissues including lung, diaphragm, liver, heart, spleen and brain in vitro. The primer was proved to specifically determine T gondii in blood and tissues in vitro but it did not detect Neospora caninum used as a negative control. DNA of T. gondii was effectively extracted by freezing, thawing and grinding twice both tissues mixed with T gondii in vitro and in experimentally infected pig's tissues. PCR detected specific DNA in the blood of experimentally infected pigs at 108 hrs and 120 hrs post-infection, it was the same time that the pigs showed fever and parasitaemia. In case of tissue, specific DNA was, however, detected only lung from experimentally infected pigs. Even though the duration of acute phase was from 3 to 7 days post-infection, but the latex agglutination test (LAT) results appeared from 8 days post-infection. A comparison of sensitivity in determining T gondii in blood samples between PCR and MI, PCR positive rate ranged from 25 to 33.3%, but that of MI covered from 75 to 100%.

• Journal of Embryo Transfer
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• v.23 no.2
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• pp.115-118
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• 2008

Glycerol is the cryoprotectant most frequently used to freeze semen in several of species. The objective of the present study was to compare the effect of three different glycerol concentrations (4, 6 or 8%, v/v) on frozen-thawed dog sperm survival rate. Ejaculates from 9 dogs collected by digital manipulation were pooled and assessed by macroscopic and microscopic criteria. Semen was divided into 3 aliquots, which were centrifuged and the sperm pellets rediluted with first Tris-glucose-citric acid extender. After 1 h cooling at $4^{\circ}C$, second extender containing 4, 6 or 8% glycerol was added, respectively. The semen was loaded into 0.25 ml straws and frozen and stored in liquid nitrogen and thawed. Sperm vigor, live:dead spermatozoa ratio using HOS test, and sperm morphology using $Spermac^{(R)}$ stain were evaluated. After thawing, there were no significant differences among groups in vigor, viability and morphology. In conclusion, the three glycerol concentrations (4, 6 or 8%) can be used successfully in cryopreservation of canine semen. Therefore the use of 4% glycerol in the extender has less toxic effect and reduces of freezing injuries.