• Journal of Pharmaceutical Investigation
• /
• v.35 no.5
• /
• pp.337-341
• /
• 2005

The purpose of the study was to prepare the pegylated liposome carrying plasmid DNA with optimal encapsulation efficiency. Plasmid DNA (pCEP4 clone 790, 10.6 kb) was entrapped in the pegylated liposome composed of neutral lipid, POPC (l-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), cationic lipid, DDAB (dimethyl dioctadecyl ammonium bromide) and anionic lipids, DSPE-PEG 2000 (distearoyl phosphatidyl ethanolamine polyethylene glycol 2000) and DSPE-PEG 2000-maleimide by freezing/thawing method. Free plasmid DNA was separated from the encapsulated one by Sepharose CL-4B column chromatography. The DNA amount encapsulated into the pegylated liposome was increased as cationic lipid concentration, initial amount of plasmid DNA and total lipid amount were increased.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.52 no.2
• /
• pp.19-25
• /
• 2010

This study was conducted to determine the resilient modulus (Mr) and the unconfined compressive strength (UCS) of two recycled roadway materials such as recycled pavement material (RPM) and road surface gravel (RSG) with or without cement kiln dust (CKD). The recycled materials were blended with two CKD contents (5, 10 %) and 28 day curing time. Mr and UCS tests were also conducted after 10cycles of freezing and thawing to asses the impact of freeze-thaw cycling. Mr was determined conducting by the laboratory test method described by NCHRP 1-28A. Stabilized RPM and RSG had a modulus and a strength higher than unstabilized RPM and RSG. Mr and UCS of RPM and RSG mixed with CKD increased with increasing CKD content. The results indicated that the addition of CKD could be improved the strength and the stiffness of RPM and RSG. Therefore, RPM, RSG and CKD could be used as an effective materials in the reconstruction of roads.

• Proceedings of the Korea Society for Simulation Conference
• /
• 2001.10a
• /
• pp.329-334
• /
• 2001

Many of past studies using physically based numerical climate models indicate that increases in atmospheric $CO_2$could enhance summer dryness over continental region in middle-high latitudes. However the models used in those studies do not take account of permafrost in high latitudes. We have carried out a set of experiments applying a version of global climate model that can reproduce realistic distribution of the permafrost. From the results, it is indicated that permafrost functions as a large reservoir in hydrologic cycle maintaining dry, hot summer over continents in northern middle-high latitudes, and that the $CO_2$warming would reduce this function by causing climatological thawing of permafrost, which would result in moister and cooler summer, and warmer winter in the same region. The present study indicates that an inclusion of very simple description of soil freezing process can make a large difference in a model simulation.

• Journal of Industrial Technology
• /
• v.24 no.A
• /
• pp.157-164
• /
• 2004

Air voids in hardened concrete have an important influence on concrete durability such as resistance of freezing and thawing, permeability and surface scaling resistance. Linear traverse method and point count method in ASTM standard method have been widely used to estimate the air void system in hardened concrete. However, these methods are not used at present time, because they are is exhausted much time and effort. In previous study, air voids system of concrete was estimated by spacing factor. The purpose of this study organizes image analysis method by analyzing air contents, air voids distributions by diameters, air voids system as well as spacing factors after hardened concrete. The experimental variables institute of depth of specimen(top, middle, bottom), air contents(AE contents 0, 0.01, 0.03%).

• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.04a
• /
• pp.415-420
• /
• 1999

Dam concrete should have sufficient durability in wet and dry repetition and abrasion due to water lever variance and also in freezing and thawing resistance as well as water penetration capacity. This study presents various experimental performance to enhance the durability of face slab concrete in concrete face rockfill dam by varying the fly ash substitution of 0%, 10%, 15% and 20% in cement quantity. The effect on durability corresponding to the increasing amount of fly-ash was evaluated and the optimum quantity of fly-ash subtitution was finally recommended. The results show that 15% fly-ash substituion was found out to be an optimum quantity and demonstrated an excellent performances in durability.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2000.10a
• /
• pp.433-438
• /
• 2000

The purpose of this study is to examine the resistance of water-permeable concretes to freezing and thawing action. The water-permeable concretes with cement-aggregate ratio of 1:5.5(by weight) and two kinds of admixture content [SP : superplasticizer(0, 1.0%), HPAE : high performance air entraining agent(0.5, 1%)] used OPC(ordinary portland cement) as binder were prepared, and then tested for relative dynamic modulus of elasiticity, mass change, length change and durablity factor. It's been concluded from the test results that the superior relative dynamic modulus of elasiticity and durability factor of water-permeable concretes were obtained at superplaciticizer 1.0% after 300 cycles. The water-permeable concretes used superplasiticizer 1.0% having relative durability factor of 88% after 300 cycles.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2005.11a
• /
• pp.283-286
• /
• 2005

Recently, the deterioration of concrete structures have been increased by the damage from salt, carbonization, freezing & thawing and the others. Reinforcement corrosion is the principal cause of deterioration of reinforced concrete. Unfortunately, full bond is assumed in all existing shear models, a condition which is often not fulfilled when assessing damaged structures. It is therefore very important to increase the understanding of how reduced bond influences the load carying capacity particularly for shear. Therefore in this study an equation is proposed to evaluate the residual shear strength considered deterioration.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2005.11a
• /
• pp.563-566
• /
• 2005

Concrete structures have been damaged by salt, carbonization, freezing and thawing and the others. Therefore, it is needed to protect durability and performance according to the appropriate materials and methods in the concrete structures. In general, several types of polymer and silicate are used as protecting deterioration agents of concrete structures, but these agents have many problems because of low durability and properties. In this study, It developed the deterioration restraining agent using polycondensed silicate and monomer that can block a deterioration cause such as $CO_2$ gas, salt and water from the outside and enhance waterproofing ability by reinforcing the concrete surface when applying it to concrete structures. Also, it developed the systems for improving concrete performance using a deterioration restraining agent.

• Journal of the Korea Concrete Institute
• /
• v.12 no.3
• /
• pp.11-19
• /
• 2000

It has been reported that few manufacturers of cement mortar for precast products use chemical and mineral admixture due to the absense of restrictions related to the application of admixture and the poor manufacturing facilities. Therefore, this paper is intended to contribute to the improvement of quality by investigating the properties of cement mortar for precast products using fly ash, blast furnace slag and AE water reducing agent. According to the test results. it was found that the cement mortar products using fly ash and AE water-reducing agent had better qualities than those of ordinary portland cement.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2006.05b
• /
• pp.241-244
• /
• 2006

The basic prediction model was constructed to obtain optimal maintenance method for concrete structure under marine environment by exploring the mechanism of mono and combined deterioration in lab. This model was planned to be upgraded with data acquired from several exposure specimens under same environment as structures. The computer program developed to give useful guidance observer would be improved. Several repair materials and repair construction methods applied to exposure specimens will be tested for its performance of prohibit salt attack and freezing & thawing action during experimental period about ten years. All of these data could be available to complete the prediction system. The manager will be able to use the system for optimal maintenance of marine concrete structures.