• Proceedings of the Korea Concrete Institute Conference
• /
• 2005.05b
• /
• pp.177-180
• /
• 2005

Decing agent with chloride promote frost damage of concrete structure in winter period. From the safetyside, however, it is difficult to stop using chloride. Therefore, the durable concrete is required from now on. In this paper, long-term freezing and thawing test was carried out using the freezing water containing $CaCl_{2}$ to consider the concrete scaling according to the kind of binders, such as OPC, Flyash, Slag+Fa. and Slag As a result, it was found that the degree of scaling is different with containing slag being considered to prevent the penetration chloride ion and according to each binders.

• Journal of Embryo Transfer
• /
• v.29 no.1
• /
• pp.13-19
• /
• 2014

This study was carried out to study the survival rate of thawed Hanwoo embryos frozen by the slow-rate freezing or the cryotop vitrification method. Hanwoo cumulus-oocyte complexes were recovered from ovaries at a slaughter house, matured for 20~22 hours, fertilized with Hanwoo semen for 5~6 hours, and cultured for 7~9 days in $38.5^{\circ}C$, 5% $CO_2$ incubator. For freezing, Day 7~9 blastocysts were collected. Embryos for the slow-rate freezing were equilibrated in 1.8 M ethylene glycol (EG) with Dulbecco's phosphate-buffered saline (D-PBS). Programmable cell freezer was precooled down to $-7^{\circ}C$, and the straw was seeded during 8 minutes-holding time, and was cooled to $-35^{\circ}C$ at the cooling rate of $0.3^{\circ}C/min$, and then was plunged and stored in liquid nitrogen. Embryos for the cryotop vitrification were treated in TCM199 with 0.5 M sucrose, 16% EG, 16% dimethylsulfoxide (DMSO). Embryos were then loaded individually onto cryotop and plunged directly into liquid nitrogen. The survival rates of embryos frozen by these two freezing methods were evaluated at 12 to 24h post-thawing. The survival rates of frozen/thawed Hanwoo embryos by the cryotop vitrification method ($56.86{\pm}26.53%$) were slightly higher than those by the slow-rate freezing method ($55.07{\pm}26.43%$) with no significant difference. Using the cryotop vitrification and the slow-rate freezing of Hanwoo blastocysts on Day 7 following in-vitro fertilization (IVF) treatment, the survival rates of frozen/thawed Hanwoo embryos were $72.65{\pm}18.3%$ and $79.06{\pm}17.8%$, respectively. The survival rates by the cryotop vitrification were higher than those by the slow-rate freezing on both Day 8 and 9 with significantly higher survival rate on Day 9 (p<0.05). Using the cryotop vitrification and the slow-rate freezing of Hanwoo embryos to compare between three different blastocyst stages, the survival rates of the blastocyst stage embryos were $66.22{\pm}18.8%$ and $45.76{\pm}12.8%$, respectively with higher survival rate by the vitrification method (p<0.05). And the survival rate of expanded blastocysts was higher than those of early blastocysts and blastocysts in two freezing methods with significantly higher survival rate by the slow-rate freezing method (p<0.05).

• Journal of Animal Science and Technology
• /
• v.55 no.5
• /
• pp.417-425
• /
• 2013

We sought to provide a method for freezing and preserving primordial germ cells, or an avian germ cell of a bird, as a material for developmental engineering or species preservation. The aim of this study was to compare the efficacy of slow freezing with a vitrification method for the cryopreservation of chicken primordial germ cells (PGCs). PGCs obtained from the germinal gonad of day 5.5-6 day (stage 28) cultured chick embryos, using the MACS method, were classified into two groups: slow freezing and vitrification. We examined the viability of PGCs after Cryopreservation. Four freezing methods were compared with each other, including the following: Method 1: The PGCs were frozen by a programmed freezer in a plastic straw, including 2.0 M ethylene glycol (EG) as cryoprotective additive (slow freezing) Method 2: The PGCs were vitrified in a plastic straw, including 8.0 M EG, plus 7% polyvinylpyrrolidone (PVP) (rapid freezing). Method 3: The slow freezing was induced with a cryotube including 2.0 M EG Method 4: The PGCs were frozen in a cryotube including 10% dimethyl suloxide (DMSO) (rapid freezing). After freezing and thawing, survival rates of the frozen-thawed PGCs from Method 1 to 4were 76.4%, 70.6%, 80.5% and 78.1% (p<0.05), respectively. The slow freezing ($-80^{\circ}C$ programmed freezer) method may provide better survival rates of frozen-thawed PGCs than the vitrification method for the cryopreservation of PGCs. Therefore, these systems may contribute to the cryopreservation of a rare avian species.

• Journal of the Korean Society of Industry Convergence
• /
• v.17 no.1
• /
• pp.7-14
• /
• 2014

In this study, characteristic of elastic paving materials in bicycle road using polyurethane were studied experimentally. As a results, following their good result was obtained. Bicycle road packaging materials are flexural strength g test, ratio of mass change after freezing and thawing, repulsion elasticity test, water Permeation coefficient test and slip resistance test results showed good performance.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.10a
• /
• pp.263-266
• /
• 1999

This study aims to make high performance concrete for normal strength using crushed stone fines to control high strength of the high performance concrete. According to the experimental results, when crushed stone fines are increased every 10%, 15% of compressive strength is decreased, and 5% of drying shrinkage is increased, compared to normal high performance concrete. Also, high performance concrete has been evaluated to have good durability factor more than 100% in the 480cycle of freezing and thawing test, without regard to using AE and crushed stone fines.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.10a
• /
• pp.647-650
• /
• 1999

Deterioration of agricultural hydraulic structures(AHS), which are under harsh environmental conditions, is more sever than other ordinary structures. To investigate the deterioration factors of AHS, various physical and chemical analyses are performed. The porosity of AHS increases more rapidly than ordinary structures because they are subject to frequent water permeation and water-soluble materials are easily emitted to surface area. Thus, AHS are tend to be damaged by freezing and thawing more easily due to the increase of water containment inside concrete.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2001.11a
• /
• pp.22-27
• /
• 2001

The purpose of this study is development of dry floor tile method that practically used for improving wet method's defect, with resin mat design. PE resin used mat which satisfied with bonding test, waterproof test, resistance to chemical attack test, resistance to impact test and freezing and thawing test is confirmed the basic property.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.04a
• /
• pp.27-32
• /
• 1999

This study was performed to evaluate properties of recycled concrete for roadway pavement. Recycled concretes was manufactured for the target compressive strength of 280kg/$\textrm{cm}^2$ and 180kg/$\textrm{cm}^2$ with recycled aggregate ratio of 0%, 20%, 40%, 60%, 80%, respectively. Laboratory experiment was conducted for testing properties of fresh concrete and concrete strength at curing 28days and durability by freezing and thawing treatment. The study result presented a maximum replacement ratio of recycled material.

• Journal of the Korean Ceramic Society
• /
• v.28 no.7
• /
• pp.509-516
• /
• 1991

The effect of various polymers on the freeze-thaw resistance of hardened cement mortar was investigated. For this study, styrene butadiene rubber (SBR), ethylene vinyl acetate (EVA), polyvinyl alcohol (PVA) were used to prepare cement mortar specimen, and then freeze-thaw experiment was carried out. By adding SBR adn EVA to the specimen, the freeze-thaw resistance of specimens was improved, but when PVA was added to the specimen, its freeze-thaw resistance was lowered. Particularly, the specimens which were added 5, 10% of SBR and 5% of EVA showed excellent freeze-thaw resistance in the salt environment.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1995.10a
• /
• pp.30-34
• /
• 1995

There are carbonatiion, salt attack, freezing & thawing and alkali-aggregate reactions as the cautions of durability lowering of concret structure. Generally, these cautions complicatedly lower the durability of structure and among these cautions the most serious problem is salt attack and carbonation. This study is intending to get the carbonation phenominon, that among cement calcium hydroxides change into calcium carbonate by responding to carbon mitrogen in the air.