• Title/Summary/Keyword: freezing, thawing

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Effects of Various Thawing Conditions on Quality Characteristics of Frozen Garlic (해동조건에 따른 냉동마늘의 품질 특성)

  • Park, Jong Woo;Kim, Jinse;Park, Seok Ho;Choi, Dong Soo;Choi, Seung Ryul;Kim, Yong Hoon;Lee, Soo Jang;Kim, Hayun
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.5
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    • pp.893-901
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    • 2015
  • This study investigated the effects of thawing conditions on physiological activities and quality of peeled garlic. Peeled frozen garlic was analyzed after thawing at low temperature ($4^{\circ}C$), room temperature ($20^{\circ}C$), tap water ($20^{\circ}C$), radio frequency of 27.12 MHz, and 2.45 GHz in a microwave. As a result, the time required to thaw garlic to $0^{\circ}C$ by various thawing methods was shortest at2.45 GHz in a microwave, followed by $20^{\circ}C$ tap water, radio frequency of 27.12 MHz, $20^{\circ}C$, and $4^{\circ}C$. Microwave thawing was faster than other methods, but it resulted in significant non-uniformity of heating. The hardness of peeled garlic significantly decreased upon freeze-thawing, whereas it showed improved hardness upon radio-frequency thawing. Total color difference in garlic increased upon freeze-thawing, and it was not improved by various thawing methods. Antioxidant activities were determined for DPPH radical scavenging ability, SOD-like activity, and reducing power. Total phenolic compounds and flavonoids in garlic extract were measured as $3.222{\pm}0.214{\mu}g$ GAE/g and $0.149{\pm}0.03{\mu}g$ QE/g, respectively. The content of total phenolic compounds was significantly reduced by 2.45 GHz microwave thawing ($1.90{\pm}0.02{\mu}g$ GAE/g); however, flavonoid contents were slightly reduced under freezing and thawing conditions. The DPPH radical scavenging ability of garlic extracts was not affected by thawing methods; however, SOD-like activity and reducing power were slightly reduced by freeze-thawing. These results indicate that physiological activities were not improved by radio-frequency thawing; however, thawing time and maintain hardness were reduced compared with conventional thawing methods.

Durability Characteristics of Controlled Low-Strength Materials using Bottom Ash (Bottom ash를 함유한 저강도 고유동 재료의 내구성능)

  • 원종필;이용수
    • Proceedings of the Korea Concrete Institute Conference
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    • 2001.11a
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    • pp.1203-1206
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    • 2001
  • The main intent of this research is to determine the feasibility of utilizing bottom ash as CLSM(Controlled Low-strength Materials). The durability tests including permeability, repeated wetting and drying, freezing and thawing for bottom ash CLSM were conducted. Laboratory test results indicated that CLSM using bottom ash has acceptable durability performance.

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Experimental Study of Freezing and Thawing Characteristics of Concrete Influenced by Air Content and Water Cement Ratio. (공기량 및 물-시멘트비에 따른 콘크리트의 동결융해 특성에 관한 실험적 연구)

  • 오병환;한승환;이용호
    • Proceedings of the Korea Concrete Institute Conference
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    • 1993.10a
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    • pp.91-96
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    • 1993
  • 본 연구의 목적은 콘크리트의 열화손상을 주는 요인 중 특히 수분의 공급이 충분한 동결융해작용에 대한 영향인자에 대한 실험적 연구이다. 콘크리트의 열화에 영향을 주는 여러 영향인자 중에서 주로 공기량 및 물-시멘트비를 다루었다.

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Development of a Simple Cell Lysis Method for Recombinant DNA Using Bacteriophage Lambda Lysis Genes

  • Jang, Bo-Yun;Jung, Yun-A;Lim, Dong-Bin
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.593-596
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    • 2007
  • In this study, we describe the development of a simple and efficient method for cell lysis via the insertion of a bacteriophage lambda lysis gene cluster into the pET22b expression vector in the following order; the T7 promoter, a gene for a target protein intended for production, Sam7 and R. This insertion of R and Sam7 into pET22b exerted no detrimental effects on cellular growth or the production of a target protein. The induction of the T7 promoter did not in itself result in the autolysis of cells in culture but the harvested cells were readily broken by freezing and thawing. We compared the efficiency of the cell lysis technique by freezing and thawing to that observed with sonication, and determined that both methods completely disintegrated the cells and released proteins into the solution. With our modification of pET22b, the lysis of cells became quite simple, efficient, and reliable. This strategy may prove useful for a broad variety of applications, particularly in experiments requiring extensive cell breakage, including library screening and culture condition exploration, in addition to protein purification.

A Study on the Sliding Characteristics of Infilling-joint Surface (충전절리면의 미끄러짐특성에 관한 연구)

  • Lee, Jung-Yub;Park, Yeong-Mog;Kim, Jae-Seok;Koo, Ho-Bon;Baek, Yong
    • Proceedings of the Korean Geotechical Society Conference
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    • 2003.03a
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    • pp.499-502
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    • 2003
  • The purpose of this study is to understand the sliding characteristics of the infilling-joint surface using the new devised shear test apparatus with changeable slope for the original infilling materials and the infilling materials experienced cyclic freezing-thawing processes. Three types of the mother rock classified as the igneous rock, the metamorphic rock and the sedimentary rock and the infilling materials were collected for laboratory test. The cohesion according to the slope change of the rock joint shows large variation within ${\pm}$5 degrees but the internal friction angle shows appears the linear decreasing tendency. It is confirmed that the affecting factor of slope change of rock joint at the behavior of rock mass is larger than that of the infilling thickness. Test results show that the cohesion and the internal friction angle in 100 times of cyclic freezing-thawing processes are decreased about 50 percent compared with original one. A further study using various infillings materials would lead to a better understanding of the failure mechanism of rock mass by slope change of rock joint.

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Evaluation of Horizontal Force on Pile Shaft Surrounded by Vertical PET Aggregate Layer for Fluid Machinery Structure Installation in Cold Region's Plant (동토 플랜트 유체기계 구조물 설치를 위한 PET 골재적용 말뚝의 주면작용 수평력 평가)

  • Ji, Subin;Jang, Sung Min;Hwang, Soon Gap;Lee, Kicheol;Kim, Dongwook
    • The KSFM Journal of Fluid Machinery
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    • v.19 no.3
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    • pp.43-47
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    • 2016
  • Pile foundations constructed on extremely cold regions cause serviceability problems of superstructures from repeated actions of ground freezing and thawing. Oil sand module plants are mainly constructed on seasonal frozen ground. Due to the freezing and thawing actions of grounds, vertical movements of piles have been observed. To solve these erratic pile movement problems, thin vertical layer of PET aggregates is installed around the pile shaft to prevent potential unfavorable pile movements. There is no known method to calculate "thin PET aggregate layer" -surrounded pile shaft resistance (capacity) against vertical loads; therefore, this experimental research is conducted. Specifically, in this study, horizontal (normal) pressures on pile shaft were assessed varying PET aggregate layer thickness based on the experiment.

Resistance of concrete made with air- and water-cooled slag exposed to multi-deterioration environments (서냉 및 급냉슬래그를 적용한 콘크리트의 복합열화 저항성)

  • Lee, Seung-Tae;Park, Kwang-Pil;Park, Jung-Hee;Park, Se-Ho
    • International Journal of Highway Engineering
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    • v.20 no.3
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    • pp.11-18
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    • 2018
  • PURPOSES : Durability of concrete is traditionally based on evaluating the effect of a single deterioration mechanism such as freezing & thawing action, chloride attack, carbonation and chemical attack. In reality, however, concrete structures are subjected to varying environmental exposure conditions which often results in multi-deterioration mechanism occurring. This study presents the experimental results on the durability of concrete incorporating air-cooled slag(AS) and/or water-cooled slag(WS) exposed to multi-deterioration environments of chloride attack and freezing & thawing action. METHODS : In order to evaluate durable performance of concretes exposed to single- and multi-deterioration, relative dynamic modulus of elasticity, mass ratio and compressive strength measurements were performed. RESULTS :It was observed that multi-deterioration severely affected durability of concrete compared with single deterioration irrespective of concrete types. Additionally, the replacement of cement by AS and WS showed a beneficial effect on enhancement of concrete durability. CONCLUSIONS : It is concluded that resistance to single- and/or multi-deterioration of concrete is highly dependent on the types of binder used in the concrete. Showing the a good resistance to multi-deterioration with concrete incorporating AS, it is also concluded that the AS possibly is an option for concrete materials, especially under severe environments.

CLSM [Confocal Laser Scanning Microscope] Observation of the Surface Roughness of Pressurized Rock Samples During Freeze/Thaw Cycling

  • Kim, Hye-jin;Choi, Junghae;Chae, Byung-gon;Kim, Gyo-won
    • The Journal of Engineering Geology
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    • v.25 no.2
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    • pp.165-178
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    • 2015
  • Physical and chemical weathering degrades rock, affecting its structural properties and thus the stability of stone buildings or other structures. Confocal laser scan microscopy (CLSM) is used here to observe temporal changes in the surface roughness of rock samples under simulated accelerated weathering. Samples were pressurized to 50, 55, or 70 MPa using a pressure frame, and subjected to freeze/thaw cycling controlled by a thermostat. The temperature was cycled from -20℃ to 40℃ and back. After each 20 cycles, CLSM was used to assess the change in surface roughness, and roughness factors were calculated to quantify the progression of the surface condition over time. Variations in cross-section line-roughness parameters and surface-roughness parameters were analyzed for specific parts of the sample surfaces at 5× and 50× magnification. The result reveals that the highest and lowest values of the roughness factors are changed according to elapsed time. Freezing/thawing at high pressure caused larger changes in the roughness factor than at low pressure.