• Magazine of the Korea Concrete Institute
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• v.27 no.5
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• pp.30-35
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• 2015

• Korean Journal of Animal Reproduction
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• v.12 no.2
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• pp.84-90
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• 1988

This study was done with mouse embryos to determine effects of the freezing media with or without 10% sucrose, and seeding methods (pincette, no seeding, liquid nitrogen gas phase and copper wire coiled straw) on embryo survival were determined using the FDA test. The summarized results are the following. 1. The FDA score found with copper wire coiled straw, no seeding, pincette and liquid nitrogen gas phase was 3.6, 3.6, 3.3 and 3.0, respectively. There were no significant differences. 2. The embryo score shows higher (P<0.05) survival rate using a freezing medium with sucrose than the one without it. Among the seeding procedure, better resutls are copper wire coiled straw and no seeded. 3. The results suggest that copper wire coiled seeding no seeding be as good as seeding when the mouse embryos were frozen in a liquid nitrogen container using both the freezing and dilution media containing 10% sucrose.

• Korean Journal of Animal Reproduction
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• v.17 no.3
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• pp.201-207
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• 1993

We determined whether the ultrarapid freezing method is applicable to micromanipulated mouse embryos. One-cell mouse embryos were microinjected with MThGH gene. Nuclei from one-cell embryos of F1(C57BL$\times$CBA) mice were transplanted into enucleated one-cell embryos of ICR mice. The injected and nucleated embryos that developed to 2-cell stage were cryopreserved by ultrarapidfreezing. The embryos equilibrated in freezing medium(3 M DMSO＋0.25 M sucrose＋2% FBS in PBS) were directly immersed into liquid nitrogen and then thawed in 37$^{\circ}C$ water. Development rates of the microinjected and nuclear-transplanted embryos to blastocyst stage after ultrarapidly freezing and thawing were 31% and 55%, respectively. The frozen-thawed embryos were transferred to pseudopregnant recipient, which then gave birth to 17 offsprings. Twelve(14% of the transferred embryos) and five(20%) offsprings were derived from microinjected and nuclear-transplanted embryos, respectively. The results indicate that the DNA injected and nuclear-transplanted mouse embryos are cryopreservable at 2-cell stage by ultrarapid freezing method.

• Proceedings of the Korean Geotechical Society Conference
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• 2002.03a
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• pp.329-336
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• 2002

The earth structures and in-ground LNG tank, and buildings can be constructed with using artificial freezing method on the reclaimed land. In this study, upon freezing a saturated soil in a closed-system from the top, a considerable pressure was developed. The pressure is the result of the surface energy of a curved ice-water interface. The most significant of these parameters will have the greatest effect on the classification. In order to establish frost-susceptibility criteria based on frost heaving expansion pressure, more soils have to be tested. This study was initiated to investigate the soils frost heaving expansion pressure and moisture characteristics resulting from freezing and freezing-thawing cycle process. Weathered granite soils, sandy soil, sandy soil were used in the laboratory freezing test subjected to thermal gradients under closed- systems.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.10a
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• pp.241-244
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• 1999

This study is represented the strength and freeing-thawing properties of recycled aggregate concrete mixed fly ash by experiment. The experimental variables are the substitution ratio of recycled aggregate and the mixing ratio of fly-ash. For each specimens, there were tested compressive strength and freeze-thaw resistance. It is able to find from the experimental result that the recycled aggregate concrete has good properties as general concrete on the compressive strength and the durability.

• Magazine of the Korean Society of Agricultural Engineers
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• v.21 no.1
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• pp.63-77
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• 1979

Six kinds of weathered granite soils whose degree of weathering and mineral compo- sitions are different, were tested in order to improve the soil-cement. by performing compression test, durability (freezing-thawing) test and mesurement of shrinkage are made. From result of the tests as mentioned above, the following conclusions are drawn. The unconfined compressive strength of seondary additives containing soil-cement mixtures and their resistance against freezeing-thawing are more increased and shrinkage is more decreased than soil-cement mixtures only in case opitimun quantity of additives are added to soil-cement mixtures, and according as types of soils.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.6C
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• pp.275-284
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• 2012

The Antarctic continent exposed to strong wind, very low temperature, and extremely dry condition. The freezing-thawing cycles under this extreme environment change the mechanical characteristics of rocks near the ground surface. To investigate the effect of freezing-thawing cycles under the extreme environment understand on geotechnical properties of rocks, rocks from the Antarctica were collected from two places: (1) West Antarctic Cape Burks and (2) East Antarctic Terra Nova Bay areas. The rock characteristics of these two areas were described and compared. For Terra Nova Bay area, rock characteristics of rocks near the surface and depths exceeding 2.9 m were examined. The 'near-the-surface rocks' averages of absorption rate, P-wave velocity, and unconfined compressive strength were 0.56%, 3,717 m/s, and 109MPa, respectively; while, those values of 'deep-sited rocks' were 0.24%, 4,670 m/s, and 88MPa. From the measurements, it was found that the effects of weathering were not significant on mechanical characteristics (strength) but were pronounced on physical characteristics(absorption and P-wave velocity).

• Tunnel and Underground Space
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• v.25 no.5
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• pp.435-449
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• 2015

Physical and microstructural properties of Pungchon and Maggol limestone were investigated quantitatively during 50 cycles of artificial freezing and thawing test. There were decrease in dry weight and P,S-wave velocity, and increase in absorption rate in both rock types. Porosity, pore volume, equivalent diameter, throat thickness and pore orientation were analyzed using X-ray computed tomography images. Porosity increased, and initiation and expansion of pores were investigated as weathering progresses. Physical and microstructural variation in Maggol limestone was larger than that of Pungchon limestone because Maggol limestone has more pores and microcracks at initial state. As this study analyzes physical and microstructural properties of rock specimens comprehensively, it can be applied to further rock weathering study and can be used as fundamental data of construction and resource development in cold regions.

• Applied Chemistry for Engineering
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• v.10 no.5
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• pp.789-795
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• 1999

In this study, carbon fiber reinforced cement composites(CFRCs) reinforced with short noncircular type carbon fibers were fabricated and properties(drying shrinkage, resistance to freezing and thawing, and fracture toughness) were compared with those of the CFRCs reinforced with circular type carbon fibers. It was found that these properties greatly depended on the shape and length of carbon fibers. The drying shrinkage of CFRCs reinforced with C-type carbon fiber was superior to other CFRCs. This effect was increased with a high aspect ratio of fiber. The resistance to freezing and thawing was increased with the fiber length and fiber volume percent, but there was on remarkable effect to fiber shape. Fracture toughness and resistance to crack propagation of CFRCs reinforced with C-CFs were improved compared with other CFRCs. It was believed that the more absorption of fracture energy into the larger interface caused an increase in fracture toughness and resistance to crack propagation.

• Clinical and Experimental Reproductive Medicine
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• v.19 no.2
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• pp.163-168
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• 1992

This study was carried out to set up the ovum bank for ovum donation and to determine the best freezing method for human immature oocytes. Human immature follicular oocytes were cryopreserved by slow freezing and rapid thawing method. Immature follicular oocytes were treated by propanediol(PROH) solution by 2 and 4 step method in protocols A & B, respectively. In protocol C, immature oocytes were exposed to sucrose prior to treatment of PROH by 4 step method. We compared survival rate, maturation rate, and fertilization rate of immature oocytes among three protocols. Results were as follows. 1. Oocytes treated by the protocol C showed the highest survival rate( 70.3 %) and maturation rate(34.6%) after thawing. 2. Survival rate of oocytes treated by the protocol C was significantly higher than that of the protocol B after thawing(p<0.05). In conclusion, treatment of oocytes with sucrose prior to expose PROH was the best freezing method. Sucrose may have reduced the toxic effect of cryoprotectant to oocytes. We failed to induce fertilization of oocytes, which were treated by any protocols, by conventional insemination method, but obtained 28.8% fertilization rate by using partial zona dissection(PZD) method. This result suggests that micromanipulation(PZD) of the thawed oocytes before insemination will improve the fertilization rate.