• 한국세라믹학회:학술대회논문집
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• 한국세라믹학회 2002년도 추계총회 및 연구발표회 초록집
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• pp.151.1-151
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• 2002

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.390-393
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• 2000

재조합 발광미생물인 UV2와 YH9을 보존제로서 trehalose를 혼합한 현탁액을 바로 동결건조한 것과 이 현탁액을 $30^{\circ}C$ incubator에서 15분 동안 진탕배양한 후 동결건조한 것과 trehalose가 첨가된 배지에 약 $6{\sim}7$시간 정도 배양한 배양액을 동결건조한 결과, trehalose를 첨가한 후 15분 동안 진탕배양했을 경우가 UV2는 0.08M의 이상에서 약 45.0%, 50.0%, YH9은 0.16M에서 48.7%, 54.1%로 회복율을 가장 높이는 최적의 조건으로 조사되었다.

• Journal of Pharmaceutical Investigation
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• 제37권1호
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• pp.51-55
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• 2007

Solid lipid nanoparticles (SLNs) have been regarded to behave similar to the vegetable oil emulsions because emulsions of lipid melts are formed before lipid droplets being solidified to turn into SLNs. Compared to lipid emulsion, however, it has been more difficult to obtain stable SLNs and needs more extensive considerations on stabilizer and manufacturing process. In the present study, we tried to prepare phosphatidylcholine-based trymyristin (TM) SLNs using high pressure homogenization method and optimize the manufacturing variables such as homogenization pressure, number of homogenization cycles, cooling temperature, co-stabilizer and freeze-drying with cryoprotectants. Nano-sized TM particles could be Prepared using egg Phosphatidylcholine and pegylated phospholipids ($PEG_{2000}$PE) as stabilizers. Based on the optimization study, the dispersion was manufactured by homogenization under the pressure of 100 MPa for more than 5 cycles, and solidifying the intermediately formed lipid melt droplets by dipping in liquid nitrogen followed by thawing at room temperature. In addition, TM SLNs could be freeze-dried and then redispersed easily without significant particle size changes after freeze drying with 10% and 12.5% sucrose or trehalose. The TM SLNs established in this study can be used as delivery system for drugs and cosmetics.

• 한국약용작물학회지
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• 제22권6호
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• pp.497-503
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• 2014

In order to produce the high quality of dried-ear mushroom, various drying methods such as hot-air drying at $40{\sim}80^{\circ}C$, freeze drying and drying in vinyl house were carried out. Drying hours of hot-air drying, freeze drying and drying in vinyl house were 12.5~21.5, 36.0 and 72.0 hrs, respectively. Vitamin $D_2$ content of sample was the highest as $6.77{\mu}g/g$ DW in drying in vinyl house and then followed by freeze drying as $5.90{\mu}g/g$ DW and hot-air drying as $1.89{\sim}5.01{\mu}g/g$ DW. After dry, external appearance and color of mushrooms applied hot-air drying and drying in vinyl house were better than freeze-dried one. After rehydration, water uptake of sample in drying in vinyl house and hot-air drying at $50{\sim}60^{\circ}C$ were 17.8 and 19.3~21.0 times, respectively. The methods of drying in vinyl house and hot-air drying at $50{\sim}60^{\circ}C$ also led to high hardness, good shape and resilience. As the results of production of dried-ear mushroom with high quality, we suggest that the best method for drying is the drying in vinyl house due to not only high vitamin $D_2$ content, good external appearance and color after drying but also high hardness and good shape after rehydration.

• 한국식품조리과학회지
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• 제19권3호
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• pp.318-323
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• 2003

본 연구는 미역과 된장, 멸치 엑기스, 다시마 엑기스, 바지락 엑기스, 파, 감자, 양파가루, 마늘가루를 이용하여 1조각이 1인분이 되도록 블록을 만들어 동결 건조시킨 후 이화학적 특성을 측정한 결과는 다음과 같다. 일반성분의 경우 제품 100 g당 조 단백의 경우 녹차 미역 된장제품에 비하여 일반 미역 된장제품의 단백질 함량이 약간 높게 나타났으며, 조 지방 함량은 일반 제품이 녹차 제품보다 1% 정도 함량이 많았으며, 탄수화물 함량은 일반 미역 된장제품보다 녹차 미역 된장제품이 약간 높게 나타났다. 회분 함량은 일반 미역 된장 제품이 녹차제품에 비해 높게 나타났다. 유리아미노산 구성에서 두 제품 모두 많은 양을 나타낸 아미노산은 alanine, phenylalanine, hydroxyproline, valine, leucine, isoleucine, Iysine 등이었으며, 대부분이 일반 미역 된장제품에 비하여 녹차 미역 된장제품의 아미노산함량이 높게 나타났다. 지방산의 조성에서 포화지방산, 단일 불포화지방산과 다가 불포화지방산의 비율은 두 제품간의 차이가 별로 없었으며, 포화지방산중에서는 palmitic acid가 가장 높게 나타났고, 단일불포화 지방산은 oleic acid, 다가 불포화지방산은 linoleic acid가 가장 높은 비율을 나타냈다. 제품의 포화 지방산과 불포화지방산의 비율은 26:74로, 불포화지방산은 단일불포화지방산에 비해 다가 불포화지방산의 비율이 두배 이상 높게 나타났다. 미역 된장제품에서 가장 많은 함량을 나타낸 유기산은 oxalic acid, 다음으로 tartaric acid였으며, 대부분 녹차제품의 유기산 함량이 높게 나타났다. 관능평가에서 맛, 색, 전반적인 기호도에서는 유의적인 차이가 없었으나, 냄새의 경우 일반 시판 된장제품이 녹차제품에 비하여 유의적으로 높게 나타났다 본 연구제품의 개발로 바쁜 현대인들 특히 맞벌이, 독신자들에게 건강을 증진시키는 효과를 가지면서 간편한 방법으로 미역 된장국을 끓여 먹을 수 있는 상품을 제공하고자 하였다.

• 대한수의학회지
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• 제12권1호
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• pp.51-58
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• 1972

Hemagglutinating antigen of Toxoplasma gondii was prepared and purified by the method of a slight modification of Tsunematsu, and the preparation of the skin test antigen (toxoplasmin) was made by means of acetone-ether treatment described by Nobute et al. With these antigens the passive hemagglutionation and skin tests were performed for the diagnosis of swine toxoplasmosis by using artificially infected pigs. The results obtained were summarized as follows: 1. The hemagglutinating antibody and the skin test antibody were demonstrated one and three weeks after infection, respectively. And these antibodies were maintained over nine weeks after infection. 2. The antigenicity of hemagglutinating antigen was stable when it was kept in frozen state, while was unstable in a liquid state. 3. Freeze-dried skin test antigen (toxoplasmin) was stable for two months or more if it was kept at $5^{\circ}C$ and room temperature, but in the liquid or reconstituted state it was unstable. 4. Freeze-dried skin test antigen could be preserved without loss of antigenicity for more than two months. 5. Passive hemagglutination test could be applied effectively at the early phase of the disease process and skin test at later phase, mainly for epidemiological survey. However, by combiniation of these methods, the more accurate results could be obtained.

• 임산에너지
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• 제20권1호
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• pp.42-52
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• 2001

국내에 자생하는 몰푸레, 쇠몰푸레 그리고 들메나무의 수피를 채취하여 아세톤-물(7:3, v/v)로 추출한 후 동결건조시켜서 분말로 제조하였고 Sephadex LH-20 및 TSK 40F로 충진한 칼럼 상에서 메탄올, 에탄올 그리고 에탄올-헥산 혼합액을 용이용매로 상요하여 칼럼크로마토그래키를 실시하였다. 단리된 화합물들은 TLC로 확인한 후 NMR스펙트럼을 상용하여 정확한 구조규명을 하였고 FAB-MS로써 분자량을 측정하였다. 쿠마린 유도체들이 다량으로 포함되어 있었으며 소량의 ester형태의 화합물들도 단리 되었다. 물푸레와 쇠물푸레에서는 aesculetin, aesculin 및 fraxetin과 같은 쿠마린 화합물들과 ligstroside와 oleuropein과 같은 ester형태의 화합무들이 모두 단리 되었으며 들메나무에서는 ester형태의 화합물만 단리 되었다.

• Applied Biological Chemistry
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• 제32권4호
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• pp.332-337
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• 1989

재수화한 동결건조 즉석미반의 리올로지 성질을 통상법으로 취반한 미반과 비교하면서 조사하였다. 온도$(60{\sim}90^{\circ}C)$를 달리한 복원시간에 따른 미립 경도의 역수(연화도)는 복원온도에 상관없이 1차 반응 속도식에 따라 감소하였고, 경도반응의 속도 상수 값은 온도에 따라 증가하였으며, 이의 활성화 에너지는 6.1 kcal/g-mol이었다. 여러 온도 $(60{\sim}90^{\circ}C)$에서 복원시킨 즉석미반의 압축응력 완화 곡선은 통상법으로 취반한 미반과 마찬가지로 일반화된 Maxwell의 6요소 역학모형으로 설명되었고, 즉석미반은 취반미보다 완화되기 쉬우나 복원온도가 높을수록 탄력성이 높아지는 특성을 나타내었다.

• 한국식품영양학회지
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• 제30권2호
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• pp.251-256
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• 2017

This study aimed to assess fatty acid composition and anti-inflammatory effects, such as nitric oxide(NO) production, expression of $TNF-{\alpha}$ and interleukin-6(IL-6), of Tenebrio molitor larva using RAW 264.7 cells. The content of total fatty acid in Tenebrio molitor larva was 76.14%, which was composed of oleic acid(42.12%), linoleic acid(32.67%) etc. There was no cytotoxicity at a dose level of 0.1, 1.0, 10, and $100{\mu}g/mL$ of freeze dried Tenebrio molitor larva ethanol extract(FDTEtOH) on RAW 264.7 cells. FDTEtOH significantly decreased NO production in LPS(lipopolysaccharide)-stimulated RAW 264.7 cells in a dose-dependent manner. Also, FDTEtOH dose-dependently suppressed the expression of $TNF-{\alpha}$ and IL-6. Thus, these results showed that Tenebrio molitor larva has the potential to be used as an anti-inflammatory food to improve immunity.

• Journal of Microbiology and Biotechnology
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• 제16권3호
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• pp.443-449
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• 2006

Bacillus polyfermenticus SCD was studied for its increasing stability by encapsulation, using 2, 3, and 4% sodium alginate. In these cases, 3% alginate resulted in the maximum survival of B. polyfermenticus SCD in artificial gastric juice for 3 h. Effects of several biopolymers on the encapsulated B. polyfermenticus SCD by 3% sodium alginate were investigated. Encapsulation with 0.5% methylcellulose showed the highest survival rate for 3 h in artificial gastric juice. Therefore, the optimized encapsulation material was 3% alginate with 0.5% methylcellulose. Furthermore, the survival of encapsulated B. polyfermenticus SCD was shown to be 122%, when 1% bile salt was added. Freeze-dried encapsulation resulted in lower survival than with non-dried encapsulation. Therefore, encapsulation was the most effective when 3% sodium alginate was used with 0.5% methylcellulose, but without freeze-drying.