The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Fatty Acid Composition and Anti-inflammatory Effects of the Freeze Dried Tenebrio molitor Larva

동결건조 갈색거저리 유충의 지방산 조성과 항염증 효과

  • Kang, Mi-Sook (Dept. of Alternative Medicine, Kyonggi University) ;
  • Kim, Min Ju (Dept. of Alternative Medicine, Kyonggi University) ;
  • Han, Jung-Soon (The Graduate School of Alternative Medicine, Kyonggi University) ;
  • Kim, Ae-Jung (The Graduate School of Alternative Medicine, Kyonggi University)
  • 강미숙 (경기대학교 일반대학원 대체의학과) ;
  • 김민주 (경기대학교 일반대학원 대체의학과) ;
  • 한정순 (경기대학교 대체의학대학원) ;
  • 김애정 (경기대학교 대체의학대학원)
  • Received : 2017.01.26
  • Accepted : 2017.03.16
  • Published : 2017.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to assess fatty acid composition and anti-inflammatory effects, such as nitric oxide(NO) production, expression of $TNF-{\alpha}$ and interleukin-6(IL-6), of Tenebrio molitor larva using RAW 264.7 cells. The content of total fatty acid in Tenebrio molitor larva was 76.14%, which was composed of oleic acid(42.12%), linoleic acid(32.67%) etc. There was no cytotoxicity at a dose level of 0.1, 1.0, 10, and $100{\mu}g/mL$ of freeze dried Tenebrio molitor larva ethanol extract(FDTEtOH) on RAW 264.7 cells. FDTEtOH significantly decreased NO production in LPS(lipopolysaccharide)-stimulated RAW 264.7 cells in a dose-dependent manner. Also, FDTEtOH dose-dependently suppressed the expression of $TNF-{\alpha}$ and IL-6. Thus, these results showed that Tenebrio molitor larva has the potential to be used as an anti-inflammatory food to improve immunity.

Keywords

References

  1. Bukkens SG. 1997. The nutritional value of edible insects. Ecol Food Nutr 36:287-319 https://doi.org/10.1080/03670244.1997.9991521
  2. Carluccio MA, Massaro M, Bonfrate C, Siculella C, Maffia M, Nicolardi G, Distante A, Storelli C, DeCaterina R. 1999. Oleic acid inhibits endothelial activation : A direct vascular antiatherogenic mechanism of a nutritional component in the mediterranean diet. Arterioscler Thromb Vasc Biol 9:220-228
  3. Chunfa H, Carl F. 2015. "Lipid metabolism, apoptosis and cancer therapy". Int J Molecular Sci 16:924-949 https://doi.org/10.3390/ijms16010924
  4. Chung MY, Kwon EY, Hwang JS, Goo TW, Yun EY. 2013. Pretreatment conditions on the powder of Tenbrio molitor for using as a novel food ingredient. J Seric Entomol Sci 51:9-14
  5. Dinarello DA, Endres S, Medani SN, Meydani M, Marc KH. 1991. Interleukin-1, anorexia, and dietary fatty acid. Ann NY Acad Sci 1:332
  6. Ding C, Cicuttini F, Li J, Jones G. 2009. Targeting IL-6 in the treatment of inflammatory and autoimmune diseases. Expert Opin Investig Drugs 18:1457-1466 https://doi.org/10.1517/13543780903203789
  7. Dumitru CD, Ceci JD, Tsatsanis C, Kontoyiannis D, Stamatakis, K, Lin JH, Patriotisb C, enkins NA, Copeland NG, Kollias G, Tsichlis PN. 2000. TNF-alpha induction by LPS is regulated posttranscriptionally via. a Tp12/ERK-dependent pathway. Cell 103:1071-1083 https://doi.org/10.1016/S0092-8674(00)00210-5
  8. Folch J, Lees M, Sloane SG. 1975. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497
  9. Grossman RM, Krueger J, Yourish D, Granelli-Piperno A, Murphy DP, May LT. 1989. Interleukin-6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes. Proc Natl Acad Sci USA 86:6367-6371 https://doi.org/10.1073/pnas.86.16.6367
  10. Ha JJ, Kim BK, Yi JK, Oh DY, Kim SS, Kim TK, Park YS. 2014. Cortisol and fatty acid contents in Hanwoo meat produced by antibiotics-free rearing system. Reproductive and Developmental Biology 38:129-136 https://doi.org/10.12749/RDB.2014.38.3.129
  11. Hegsted DM, Ausman LM, Johnson JA, Dallal GE. 1993. Dietary fat and serum lipids an evaluation of the experimental data. Am J Clin Nutr 57:875-883 https://doi.org/10.1093/ajcn/57.6.875
  12. Huiqun T, Chaoqi L, Xiaohua Z, Wei W, Changcheng Z, Ding Y. 2015. "MiRNA-194 regulates palmitic acid-induced toll-like receptor 4 inflammatory responses in THP-1 cells". Nutrients 7:3483-96 https://doi.org/10.3390/nu7053483
  13. Hwang SY, Choi SK. 2015. Quality characteristics of muffins containing mealworm (Tenbrio molitor). The Korean J Culinary Research 21:104-115
  14. Kim EY, Moudgil KD. 2008. Regulation of autoimmune inflammation by pro-inflammatory cytokines. Immunol Lett 120:1-5 https://doi.org/10.1016/j.imlet.2008.07.008
  15. Kim HM, Kim JN, Kim JS, Jeong MY, Yun EY, Hwang JS, Kim AJ. 2015 Quality characteristics of patty prepared with mealworm powder. Korean J Food Nutr 28:813-820 https://doi.org/10.9799/ksfan.2015.28.5.813
  16. Kim HS, Jung CE. 2013. Nutritional characteristics of edible insects as potential food materials. Korean J Apiculture 28:1-8
  17. Kim SH, Kim KB, Noh JS, Yun EY, Choi SK. 2014. Quality characteristics of pasta with addition of mealworm (Tenebrio molitor). J Food Service Industry 10:55-64
  18. Lee JE, Lee AJ, Jo DE, Cho JH, Youn KJ, Yun EY, Hwang JS, Jun MR, Kang BH. 2015. Cytotoxic effect of Tenebrio molitor larva extracts against hepatocellular carcinoma. J Korean Soc Food Sci Nutr 44:200-207 https://doi.org/10.3746/jkfn.2015.44.2.200
  19. Liu J, Li F, Kong JL, Cao Y. 1998. Induction of immunomodulating cytokines by a new polysaccharidepeptide complex from culture mycelia of Lentinus edodes. Immunopharmacol 40: 187-198 https://doi.org/10.1016/S0162-3109(98)00043-5
  20. Marshall JC. 2001. Inflammation, coagulopathy and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med 29L S99-S106
  21. Oh YT. 2010. Roles of oleic acid and oleamide in microglial inflammatory response. Ph. D. Thesis, KyungHee Univ. Seoul. Korea
  22. Reiko H, Masshide A, Katsuko S, Hirotaka K, Masatoshi S, Masugi N, Michio T, Yoichiro I. 1998. Production of mice deficient in genes for interleukin(IL)-1 ${\alpha}$, IL-$1{\beta}$, IL-$1{\alpha}$/${\beta}$, and IL-1 receptor antagonist shows that IL-$1{\beta}$ is crucial in turpentine- induced fever development and glucocorticoid secretion. J Exp Med 187:1463-1475 https://doi.org/10.1084/jem.187.9.1463
  23. Rumpold, B. A., Schluter, O. K. 2013. Nutritional composition and safety aspects of edible insects. Mol Nutr Food Res 57:802-823 https://doi.org/10.1002/mnfr.201200735
  24. Simon E, Baranyai E, Braun M, Fabian I, Tothmeresz B. 2013. Elemental concentration in mealworm beetle (Tenebrio molitor L.) during metamorphosis. Biol Trace Elem Res 154:81-87 https://doi.org/10.1007/s12011-013-9700-1
  25. Strom M, Halldorsson TI, Mortensen EL, Torp-Pedersen C, Olsen SF. 2012. "Fish, n-3 fatty acids, and cardiovascular diseases in women of reproductive age: a prospective study in a large national cohort". Hypertension 59:36-43 https://doi.org/10.1161/HYPERTENSIONAHA.111.179382
  26. Su GL. 2002. Lipopolysaccharides in liver injuryl molecular mechanisms of Kupffer cell activation. Am J Physiol Gastrointest Liver Physiol 283:G256-G65 https://doi.org/10.1152/ajpgi.00550.2001
  27. Weatherill AR, Lee JY, Zhao L, Lemay DG, Yun HS and Hwang DH. 2005. "Saturated and polyunsaturated fatty acids reciprocally modulate dendritic cell functions mediated through TLR4", 5, J Immunol 174:5390-5397 https://doi.org/10.4049/jimmunol.174.9.5390
  28. Yoo JM, Hwang JS, Goo TW, Yun EY. 2013. Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J Korean Soc Food Sci Nutr 42:249-254 https://doi.org/10.3746/jkfn.2013.42.2.249
  29. Youn KJ, Yun EY, Lee JH, Kim JY, Hwang JS, Jeong WS, Jun MR. 2014. Oleic acid and lioneic acid from Tenebrio molitor larva inhibit BACE1 activity in vitro. Molecular docking studies. J Medicinal Food 17:284-289 https://doi.org/10.1089/jmf.2013.2968
  30. Yu JM, Hang JY, Kim HJ, Cho YH, Kim DI, Kwon OJ, Cho YJ, An BJ. 2016. Antioxidant capacity and Raw 264.7 macrophage anti-inflammatory effect of the Tenebrio molitor. Korean Soc Food Preserv 23:890-898

Cited by

  1. 유용 미생물을 이용한 발효갈색거저리 추출물의 이화학적 특성 및 생리활성 효과 vol.28, pp.8, 2017, https://doi.org/10.5352/jls.2018.28.8.923
  2. Quality Evaluation of Perilla Seed Gangjung added with Mealworm Powder vol.17, pp.2, 2017, https://doi.org/10.20402/ajbc.2019.0278
  3. Effect of pretreatment with paste and sauce extract made using TENEBRIO MOLITOR larvae on ethanol‐damaged HepG2 cells vol.49, pp.12, 2017, https://doi.org/10.1111/1748-5967.12397
  4. Perception of Edible Insect and Insect Foods among Adults vol.18, pp.1, 2017, https://doi.org/10.20402/ajbc.2020.0001
  5. 동애등에의 항혈전 활성 vol.30, pp.4, 2017, https://doi.org/10.5352/jls.2020.30.4.386
  6. Antioxidant Activities and Nutritional Components of Cricket (Gryllus bimaculatus) Powder and Protein Extract vol.18, pp.2, 2017, https://doi.org/10.20402/ajbc.2020.0016
  7. Beneficial Effects of Insect Extracts on Nonalcoholic Fatty Liver Disease vol.23, pp.7, 2017, https://doi.org/10.1089/jmf.2019.4536
  8. 갈색거저리(Tenebrio molitor) 유충의 산화방지 성분 및 활성 vol.36, pp.1, 2017, https://doi.org/10.13103/jfhs.2021.36.1.86
  9. Nutritional Value and Anti-inflammation Activity of Misutkaru with Added Gryllus bimaculatus Powder vol.19, pp.3, 2021, https://doi.org/10.20402/ajbc.2021.0203