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Trigonopsis variabilis의 고정화 및 Cephalosporin C로부터 7$\beta$-(4-Carbohybutanamido)Cephalosporanic Acid의 전환 (Immobilization of Trigonopsis variabilis and Conversion of Cephalosporin C to 7$\beta$-(4-Caboxybutanamido)Cephalosporanic Acid)

  • 김종균;임재윤
    • 한국미생물·생명공학회지
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    • 제22권3호
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    • pp.296-303
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    • 1994
  • An immobilized Trigonopsis variabilis cells having an high activity of D-amino acid oxidase(DAO) was used to convert CPC into GL-7-ACA. The optimal pH of the reaction system was 8.0-8.5, and the optimal temperature was 40$\circ$C. When immobilized cell was used repeatedly in semi-batchwise reaction, the system retained 80% of the initial activity after used of 12 times for over 12 hours. The storage stability of the immobilized cell was maintained for 30 days at 4$\circ$C. The CPC concentration for the maximal reaction rate was about 30 mM and 40 mM for free and immobilized cells, respectively. Substrate inhibition of CPC concentration more than 50 mM was overcomed by 20~25% by immobilization. Pure oxygen supply into reaction system was most efficient in D-amino acid oxidase reaction. Continuous conversion to GL-7-ACA from CPC has been developed with an bioreactor system containing immobilized T variabilis cells. By opera- tion of the reactor for 5 hours, the average conversion yield of >80% and GL-7-ACA production of 40~45 mM per hour could be obtained.

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유성오염의 세척성에 관한 연구(제2보) -유화와 롤링업에 의한 액체유성오염의 세척성- (Studies on the Detergency of Oily Soils (Part II))

  • 김영희;정두진
    • 한국의류학회지
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    • 제19권2호
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    • pp.356-365
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    • 1995
  • study was made to investigate the emulsification and rolling-up between liquid oily soils and surfactant and its effect on the detergency. Samples used were triolein as a triglyceride, oleic acid as a free fatty acid and sodium dodecyl sUJfate(SDS) as a surfactant. The results were as follows: 1. The spontaneous emulsification occurred in the system of oleic acid alone and mixture of triolein and oleic acid contacted with 0.5% SDS solution, but it did not occurred in a case of triolein alone. 2. The stability of emulsification increased with the increase of SDS concentration. And the stability of emulsification and (-) t value increased in the order of triolein < mixture of triolein and oleic and< oleic acid. 3. The rolling-up mechanism of model oily soils easily occurred in the order of triolein< mixture of triolein and oleic acid< oleic acid, and facilitated with the increase of SDS concentration. On the other hand, the complex formation was already observed in the system of oleic and 0.5% SDS solution. 4. As compared with the detergency of triolein, the detergency of oleic acid was very high. And the detergency of mixed soil was improved with increasing ratio of oleic acid in the mixture.

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윤활시스템에서 마모메카니즘에 미치는 물리화학적 영향에 관한 연구(II) (A Study on the Effect of physico-chemical Factors in Wear Mechanism in a Lubricated Concentrated Contact (II))

  • 최웅수;권오관;문탁진;유영흥
    • Tribology and Lubricants
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    • 제4권1호
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    • pp.43-55
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    • 1988
  • A Study on the effect of the additives in lubricating oil was investigated on the basis of the thermal activated wear theory in terms of their wear behaviours, using four ballwear machine. The sample oils, which included diethyl-3, 5-di-t-butyi-4-hydroxy-benzyl phosphonate (DEP), ZDDP and TCP additives respectively, showed distinct wear characteristics depending upon the bulk oil temperature and the sliding velocity. The newly synthesized additive, viz., DEP showed excellent antiwear performance cornpared with the conventional additives, ZDDP and TCP. On the basis of the experimental results, it is reduced that the wear mechanism of the conventional additives, viz., ZDDP and TCP is the protective film formation and their antiwear capability is depending upon the shearing strength of the film formed. On the other hand, the new additive, DEP showed that the secondary activation energy was much eliminated and so, the thermal instability was reduced by the hydrogen scavenging reaction of the new additive, which was virtually an endothermic reaction process.In conclusion, a new concept of antiwear mechanism is estabilished and testified. And new chemical, which showed the function of hydrogen and free radical scavenging role, is synthesized and introduced as the new, highly antiwear effective lubricating oil additive.

호텔 한식당의 유니폼 디자인 현황에 관한 연구 (A Study of Present Circumstance of Uniform Design for Korean Restaurant Employees in Hotels)

  • 김은정;강순제
    • 복식
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    • 제54권8호
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    • pp.101-112
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    • 2004
  • The purpose of this study was to survey the present circumstance of uniform's design and to suggest a design for employees who work for Korean restaurants in Hotels. The subject of study are 10 restaurants which are located in the first class hotels in seoul. And the period of research were 2 months between November, 2003 and December, 2003. The result of research was analyzed by various methods - which consider the frequency, percentage, T-test, crosstabs, and one-way ANOVA. The results are as follows: $1.40{\%}$ of subject adopt a general style which is not traditional style and $60{\%}$ of subject do a Han-Bok or casual Han-Bok which is traditional style. 2. About satisfaction of uniforms, $50{\%}$ of people who is wearing general style has been satisfying their uniform. And $33{\%}$ of people who is wearing traditional style has only been satisfying. 3. People prefer uniform that is made of wrinkle free fabrics and has pockets. In shape of design, they want that ankle-length of trousers, 7/8 sleeves's jackets with waist line-length, and narrow ends. They prefer that a coat has a knob button and there is a zipper in a skirt.

질산치아민의 제조조건과 치아민 모노염류의 제제학적 안정성 (Synthesis Conditions of Thiamine Mononitrate and Pharmaceutical Stability of Thiamine Monosalts)

  • 박홍구
    • 약학회지
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    • 제41권5호
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    • pp.595-601
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    • 1997
  • The optimal synthesis condition based on the yield of thiamine mononitrate has been attained according to Box-wilson experimental design. The optimal condition was as follows : Molar concentration ratio of $SB1{\cdot}HCL=0.6839$, quantity of $NH_4NO_3=50.09g$, agitation velocity=51.6rpm, reaction temperature=22.8$^{\circ}C$. The stabilities of three kinds of thiamine monosalts(thiamine monoiodide, -monobromide, -monorhodanate) were investigated in sterile solution, compressed tablet and multivitamin capsule, respectively. Assay data are given to show that thiamine monosalts are not significantly higher than that of thiamine mononitrate in sterile solutions and the addition of an acidic stabilizer is unnecessary. The solutions were clear and free of precipitate and didn't discolored after storage at room temperature and 40$^{\circ}C$ for 6 months. The tablets and capsules were assayed initially and at predetermined intervals during storage at room temperature and 40$^{\circ}C$ for 6 months. Thiamine monorhodanate was found to be more stable than other thiamine monosalts in these compressed tablets containing other vitamin of the B complex and the data indicate that thiamine monosalts was similar to that of thiamine mononitrate in the multivitamine capsules.

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The Effect of Temperature on the Corrosion of Mild Steel in H3PO4 Containing Halides and Sulfate Ions

  • Chandrasekaran, V.;Kannan, K.;Natesan, M.
    • Corrosion Science and Technology
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    • 제4권1호
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    • pp.8-14
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    • 2005
  • The corrosion behaviour of mild steel in phosphoric acid solution in the presence and absence of pollutants viz. Chloride, Fluoride and Sulfate ions at 302K-333K was studied using mass loss and potentiostatic polarization methods. The addition of chloride and sulfate ions inhibits the mild steel corrosion in phosphoric acid while fluoride ions stimulate it. The effect of temperature on the corrosion behaviour of mild steel indicated that inhibition of chloride and sulfate ions decreased with increasing temperature. The adsorption of these ions (Chloride and sulfate) on the mild steel surface in acid has been found to obey Langmuir adsorption isotherm. The values of activation energy (Ea) and free energy of adsorption ($\Delta$) indicated physical adsorption of these ions (chloride and sulfate) on the mild steel surface. The plot of $logW_{f}$ against time (days) at 302K gives a straight line, which suggested that it obeys first order kinetics and also calculate the rate constant k and half-life time $t_{1/2}$.

The Rubber Pricing Model: Theory and Evidence

  • SRISUKSAI, Pithak
    • The Journal of Asian Finance, Economics and Business
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    • 제7권11호
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    • pp.13-22
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    • 2020
  • This research explores the appropriate rubber pricing model and the consistent empirical evidence. This model has been derived from the utility function and firm profit-maximization model of commodity goods. The finding shows that the period t - 1 affects expected commodity price and expected profit of commodity production. In fact, a change in the world price of rubber in the past period led to a change in the expected price of rubber in the short run which influenced the expected rubber profit. As a result, the past-period free on board price has an entirety effect on expected farm price of rubber given an exchange rate. In addition, the rubber pricing model indicates that the profit of local farmer on rubber plant depends solely on the world price of rubber in the short run in case of Thailand. In an empirical study, it was found that a change in the price of ribbed smoke sheet 3 in Singapore Commodity Exchange significantly and positively determined the fluctuation of rubber price at the farm gate in Thailand which was consistent with the behavior of the Thai farmers. Both prices are also cointegrated in the long run. That is, the result states that the VECM is an appropriated pricing model for forecasting the farm price in Thailand.

선체(船體) 상하진동(上下振動)에 대(對)한 부가질량(附加質量)의 3차원(次元) 수정계수(修正係數)에 관(關)하여 (On the Three-dimensional Correction Factor for the Added Mass in the Vertical Vibration of the Ship.)

  • 김창열
    • 대한조선학회지
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    • 제11권2호
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    • pp.1-6
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    • 1974
  • The three-dimensional correction factor of the added mass of finite-length elliptic cylinders in vertical vibration in a free surface was calculated. This problem has already been dealt by T. Kumai[5] to contribute to analytical prediction of the three-dimensional correction factor for the added mass in vertical vibration of ships. In Kumai's work, the body boundary condition involved in the appropriate boundary value problem was approximately treated in the course of obtaining the solution. In this work, obtaining the solution derived from mathematically exact treatment of the body boundary condition, the author recalculated the three-dimensional correction factor for length-beam ratio $4{\sim}8$, beam-draught ratio $2.00{\sim}4.50$ and number of nodes from 2 to 7. And the numerical results were compared with both Kumai's results and the author's experimental data for two and three-noded vibrations of the cylinder of beam-draught ratio 2.40 The comparison of the numerical results shows that the author's are always higher than the Kumai's as expected. And the comparison of the numerical results with experimental data shows that the Kumai's numerical results have less deviation in case of two-noded vibration, and that, in case of three-noded vibration, the author's numerical results are in fairly good correspondence.

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Antioxidant Activity of Cercis chinensis and Its Protective Effect on Skin Aging

  • Na, Min-Kyun;Bae, Ki-Hwan;Hong, Nam-Doo;Yoo, Jae-Kuk;Nobuhiko Miwa
    • 대한화장품학회:학술대회논문집
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    • 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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    • pp.117-138
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    • 2003
  • Reactive oxygen species are capable of damaging biomolecules such as lipids, proteins, and DNA, which can not only lead to various diseases, but also oxidative damage resulting aging. In our previous study, Cercis chinensis (Leguminosae) showed a potent antioxidant activity. Nineteen compounds were isolated through antioxidant activity-guided fractionation. The C. chinensis extract and some of the constituents exhibited a potent antioxidant activity on the free radicals and lipid peroxidation and a notable protective effect on the t-BuOOH induced oxidative damage. In vivo test of skin damage induced by UVB irradiation, the extract of C. chinensis and a constituent, piceatannol, exhibited a significant protective effect. The life-span of the HEK-N/F cells were extended by 1.21-2.12 fold as a result of the continuous administration of 3 $\mu\textrm{g}$/ml of the C. chinensis extract and the active constituents compared to that of the control. These observations were attributed to the inhibitory effect of the C. chinensis extract and its constituents on the age-dependent shortening of the telomere. Thus, C. chinensis was demonstrated to protect the skin cells against oxidative stress and inhibit thereby the cellular aging, followed by expectation as antiaging cosmetic ingredient.

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운동프로그램이 중년비만여성의 체구성, 체력 및 지질대사에 미치는 영향 (Effects of a Exercise Program on Body Composition, Physical Fitness and Lipid Metabolism for Middle-Aged Obese Women)

  • 이군자
    • 대한간호학회지
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    • 제35권7호
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    • pp.1248-1257
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    • 2005
  • Purpose: This study was to examine the effects of an exercise program for middle-aged obese women. Method: The exercise program combined folk dance and resistance training. The subjects group consisted of 85 middle-aged obese women between 40 and 60 years of age. Three 8 week sessions consisted of a $55-80\%$ maximum heart rate (MHR) exercise for 60-90 minutes a day and 3 times a week from March to November, 2004. Data was collected through a pre- and post-exercise test before and after each session. Data was collected with Inbody, dynamometer and blood. This data was analyzed by descriptive statistics, and a paired t-test with an SPSS/PC(10.0 version) program. Results: There were significant positive changes in body weight, body fat mass, body mass index, percent body fat, muscle strength, muscle endurance, flexibility, and balance quality, but no significant positive changes in skeletal mass, fat free mass, percent abdominal fat(waist-hip ratio), visceral fat area, agility, total cholesterol, high density lipoprotein cholesterol, or triglycerides. Conclusion: This study showed that an exercise program has partially positive effects for middle-aged obese women. The results of this study show that exercise at community health centers should continue for middle-aged obese women's health.