• Journal of Korean Society of Steel Construction
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• v.16 no.5 s.72
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• pp.505-517
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• 2004

This paper presented equations on the stress evaluation of steel frame pier connections that were composed of a box beam and a circular column. The existing equations, which transformed the circular column into an equivalent box column had some problems; they underestimated a shear lag stress as the joint angle decreased, and overestimated a shear stress as the joint angel increased. Therefore, FE analyses were performed with various parameters, such as joint angle(${\alpha}$), span length-width ratio(L/B), and circular column-to-box beam stiffness ratio(${\alpha}$), and new equations on stress evaluation were proposed based on FE analyses. Furthermore, material and geometric nonlinear analyses were performed to estimate ultimate strength and to confirm the validity of the proposed equations.

• Parasites, Hosts and Diseases
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• v.46 no.4
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• pp.209-216
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• 2008

A monoclonal antibody against Toxoplasma gondii of Tg556 clone (Tg556) blotted a 29 kDa protein, which was localized in the dense granules of tachyzoites and secreted into the parasitophorous vacuolar membrane (PVM) after infection to host cells. A cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg556, and the full-length was completed by 5'-RACE of 2,086 bp containing an open reading frame (ORF) of 669 bp. The ORF encoded a polypeptide of 222 amino acids homologous to the revised GRA3 but not to the first reported one. The polypeptide has 3 hydrophobic moieties of an N-terminal stop transfer sequence and 2 transmembrane domains (TMD) in posterior half of the sequence, a cytoplasmic localization motif after the second TMD and an endoplasmic reticulum (ER) retrival motif in the C-terminal end, which suggests GRA3 as a type III transmembrane protein. With the ORF of GRA3, yeast two-hybrid assay was performed in HeLa cDNA expression library, which resulted in the interaction of GRA3 with calcium modulating ligand (CAMLG), a type II transmembrane protein of ER. The specific binding of GRA3 and CAMLG was confirmed by glutathione S-transferase (GST) pull-down and immunoprecipitation assays. The localities of fluorescence transfectionally expressed from GRA3 and CAMLG plasmids were overlapped completely in HeLa cell cytoplasm. In immunofluorescence assay, GRA3 and CAMLG were shown to be co-localized in the PVM of host cells. Structural binding of PVM-inserted GRA3 to CAMLG of ER suggested the receptor-ligand of ER recruitment to PVM during the parasitism of T. gondii.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.309-313
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• 2006

Many researchers have studied on the lightness of automobile. These researches are such as a body shell, sub frame, fuel tank, engine etc. The transmission Part is a magnitude one in the aspect of weight. A drive shaft (propeller shaft) transmits the engine power to rear differential gear assembly. It is used in the compact car that is a single drive shaft. But in the case of long body cars such as SUV (Sports Utility Vehicle), truck and large vehicle, two or three divided drive shaft are used to prevent the vibration damage from a drive shaft that has been taken high torsion and rotation. This multi-divided drive shaft structure is so heavy because it is assembled by yoke, center bearing and solid spline axis. When the rear axle move up and down, the spline shaft adjust the variation of a length between the transmission and rear axle gearbox. In this paper, it is studied in the experimental method that is a bending vibration characteristic of slip in tube shaped propeller shaft. This type propeller shaft is developed to combine the spline axis with drive shaft and can be light in weight of transmission part.

• Molecules and Cells
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• v.23 no.2
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• pp.228-238
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• 2007

Since molecular structure of hnRNP is not available in foreseeable future, it is best to construct a working model for hnRNP structure. A geometric problem, assembly of $700{\pm}20$ nucleotides with 48 proteins, is visualized by a frame work in which all the proteins participate in primary binding, followed by secondary, tertiary and quaternary binding with neighboring proteins without additional import. Thus, 40S hnRNP contains crown-like secondary structure (48 stemloops) and appearance of 6 petal (octamers) rose-like architectures. The proteins are wrapped by RNA. Co-transcriptional folding for RNP fibril of FMR1 gene can produce 2,571 stem-loops with frequency of 1 stem-loop/15.3 nucleotides and 53 40S hnRNP beaded structure. By spliceosome driven reactions, there occurs removal of 16 separate lariated RNPs, joining 17 separate beaded exonic structures and anchoring EJC on each exon junction. Skipping exon 12 has 5'GU, 3'AG and very compact folding pattern with frequency of 1 stem-loop per 12 nucleotides in short exon length (63 nucleotides). 5' end of exon 12 contains SS (Splicing Silencer) element of UAGGU. In exons 10, 15 and 17 where both regular and alternative splice sites exist, SS (hnRNP A1 binding site) is observed at the regular splicing site. End products are mature FMR-1 mRNP, 4 species of Pri-microRNAs derived from introns 7,9,15 and 3'UTR of exon17, respectively. There may also be some other regulatory RNAs containing ALU/Line elements as well.

• Journal of KIISE:Information Networking
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• v.29 no.3
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• pp.277-285
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• 2002

This paper proposes and analyzes an error control scheme for the transmission control based real-time communication, such as FDDI, TDMA, and wireless LAN, which delivers the message according to the round robin fashion after the off-line bandwidth allocation. Taking into account the time constraint of each message, the proposed error control scheme makes the receiver transmit the error report via asynchronous traffic while the sender resend the requested message via overallocated access time which is inevitably introduced by the bandwidth allocation procedure for hard real -time guarantee. The error control procedure does not interfere other real -time message transmissions. In addition, as each frame contains the size of the message it belongs, the receiver can recognize the end of completion of message transmission. This enables earlier error report to the receiver so that the sender can cope with more network errors. The analysis results along with simulation performed via SMPL show that the proposed scheme is able to enhance the deadline meet ratio of messages by overcoming the network errors. Using the proposed error control scheme, the hard real -time network can be built at cost lower than, but performance comparable to the expensive dual link network.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.135-143
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• 2011

In the Doritaenopsis hybrid, like most of the orchid species and hybrids, temperature is crucial for the vegetative-to-reproductive transition, and low temperature is required for bud differentiation. To understand the molecular mechanism of this process, an orchid GIGANTEA (GI) gene, DhGI1, was isolated and characterized by using the rapid amplification of cDNA ends (RACE) PCR technique. Sequence analysis showed that the full-length cDNA is 4,022 bp with a major open reading frame of 3,483 bp, and the amino acid sequence showed high similarity to GI proteins in Zea mays, Oryza sativa, Arabidopsis thaliana and other plants. Semi-quantitative RT-PCR revealed that DhGI1 was expressed throughout development and could be detected in roots, stems, leaves, peduncles and flower buds. The expression level of DhGI1 was higher when the plants were flowering at low temperature (22/$18^{\circ}C$ day/night) than the other growth stages. Further analysis indicated that the accumulation of DhGI1 transcripts was significantly increased at low temperature, and concomitantly, initiation of the peduncle was observed. However, DhGI1 levels were low under high temperature (30/$25^{\circ}C$) conditions, and flower initiation was inhibited. These results indicate that the expression of DhGI1 is regulated by low temperature and that DhGI1 may play an important role in inflorescence initiation in this Doritaenopsis hybrid at low temperatures.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.3
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• pp.346-353
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• 2015

Glucocorticoids (GCs) are steroid hormones regulated through responses to stress to maintain diverse metabolic and homeostatic functions. GCs act on the glucocorticoid receptor (GR), a member of the nuclear receptor family. This study identified and characterized the GR gene from the big-belly seahorse Hippocampus abdominalis designating it HaGR. The open reading frame of the HaGR cDNA was 2,346 bp in length, encoding a 782-amino-acid polypeptide with a theoretical isoelectric point of 6.26 and predicted molecular mass of 86.8 kDa. Nuclear receptors share a common structural organization, comprising an N-terminal transactivation domain, DNA-binding domain, and C-terminal ligand-binding domain. The tissue-specific mRNA expression profile of HaGR was analyzed in healthy seahorses using a qPCR technique. HaGR mRNA was expressed ubiquitously in all of the tissues examined, with the highest expression levels in kidney, intestine, stomach, and gill tissues. The mRNA expression in response to immune challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), Edwardsiella tarda, and Streptococcus iniae revealed that it is inducible in response to pathogen infection. These results suggest that HaGR is involved in the immune response of the big-belly seahorse.

• Journal of Korean Association for Spatial Structures
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• v.18 no.2
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• pp.79-86
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• 2018

Unlike other types of outdoor advertisements, rooftop signboards are installed on the roofs of buildings, rather than on their outer walls. This means that the area of a rooftop signboard is commonly larger than that of a general outdoor signboard. Moreover, as such signboards are greatly influenced by the wind, they can suffer a lot of damage from typhoons and strong winds every year. However, there is no wind load specification for rooftop signboards. In this study, wind pressure experiments were conducted to investigate the peak wind pressure on each side of rooftop signboards installed on the roofs of 5-15 story buildings in a city center. The minimum peak wind pressure coefficient was -3.0 at the bottom edges of the front and back of the rooftop signboards and -2.0 along the entire length of the sides. As the height of the rooftop signboard increased with the increasing height of the buildings, the peak value was found to be larger than the absolute peak value for the minimum peak wind pressure coefficient. The maximum and minimum peak wind pressure distributions of the rooftop outdoor signboards were influenced by the position of the signboard and the wind angle.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.7A
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• pp.964-972
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• 1999

In this paper, we proposed a simulation model to which apply the AAL 2 (AAL type 2) between BSC and MSC in the cellular mobile communication systems. We suggested the frame structure of processing the packets of short length and the scheme which multiplex to one or more ATM cell. Also, we analyzed the performance of the APR, transmission delay, and channel transmission efficiency used in the packetized voice traffic and the low bit-rate data traffic such as fax. From the simulation results, the maximum number of users are 47 users without using AAL 2 multiplexing, but the maximum number of users are 70 (Non-Overlapping scheme) users, 110 (Overlapping scheme) users, respectively. Thus, we knew that the Overlapping scheme is more efficient than the Non-Overlapping scheme. Finally, we showed that the optimum transmission buffer size is 4 ATM cells in the cellular communication systems with the bandwidth of 2 Mbps.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.1
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• pp.101-108
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• 2012

Initial shape analysis for cable-stayed bridges should be able to find optimizated initial cable forces and unstrained length that minimize deflection and bending moments of the deck and pylon. Comparison study of an improved initial force method and TCUD method for determination of initial cable forces in cable-stayed bridges is presented in this paper. For this purpose, an elastic catenary cable element and a nonlinear frame element are firstly described. And concepts and algorithm of two analysis methods are then presented. Finally to demonstrate the validity and the accuracy of two methods, numerical examples for initial state problems of cable-stayed bridges are given and compared based on these methods.