• Asian-Australasian Journal of Animal Sciences
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• v.25 no.5
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• pp.606-612
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• 2012

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1106-1112
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• 2007

This report provides the complete nucleotide sequences of the full-length cDNA encoding squalene synthase (SQS) and its genomic DNA sequence from a triterpene-producing fungus, Ganoderma lucidum. The cDNA of the squalene synthase (SQS) (GenBank Accession Number: DQ494674) was found to contain an open reading frame (ORF) of 1,404 bp encoding a 468-amino-acid polypeptide, whereas the SQS genomic DNA sequence (GenBank Accession Number: DQ494675) consisted of 1,984 bp and contained four exons and three introns. Only one gene copy was present in the G. lucidum genome. The deduced amino acid sequence of Ganoderma lucidum squalene synthase (GI-SQS) exhibited a high homology with other fungal squalene synthase genes and contained six conserved domains. A phylogenetic analysis revealed that G. lucidum SQS belonged to the fungi SQS group, and was more closely related to the SQS of U. maydis than to those of other fungi. A gene expression analysis showed that the expression level was relatively low in mycelia incubated for 12 days, increased after 14 to 20 days of incubation, and reached a relatively high level in the mushroom primordia. Functional complementation of GI-SQS in a SQS-deficient strain of Saccharomyces cerevisiae confirmed that the cloned cDNA encoded a squalene synthase.

• Journal of the Optical Society of Korea
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• v.19 no.1
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• pp.55-62
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• 2015

In this study we demonstrate ultrahigh-resolution spectral domain optical coherence tomography (UHR SD-OCT) with a linear-wavenumber (k) spectrometer, to accelerate signal processing and to display two-dimensional (2-D) images in real time. First, we performed a numerical simulation to find the optimal parameters for the linear-k spectrometer to achieve ultrahigh axial resolution, such as the number of grooves in a grating, the material for a dispersive prism, and the rotational angle between the grating and the dispersive prism. We found that a grating with 1200 grooves and an F2 equilateral prism at a rotational angle of $26.07^{\circ}$, in combination with a lens of focal length 85.1 mm, are suitable for UHR SD-OCT with the imaging depth range (limited by spectrometer resolution) set at 2.0 mm. As guided by the simulation results, we constructed the linear-k spectrometer needed to implement a UHR SD-OCT. The actual imaging depth range was measured to be approximately 2.1 mm, and axial resolution of $3.8{\mu}m$ in air was achieved, corresponding to $2.8{\mu}m$ in tissue (n = 1.35). The sensitivity was -91 dB with -10 dB roll-off at 1.5 mm depth. We demonstrated a 128.2 fps acquisition rate for OCT images with 800 lines/frame, by taking advantage of NVIDIA's compute unified device architecture (CUDA) technology, which allowed for real-time signal processing compatible with the speed of the spectrometer's data acquisition.

• Membrane Journal
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• v.18 no.4
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• pp.294-305
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• 2008

This study was focused on the investigation of the effects of membrane module configuration and the temperature of feed retentate flowing along with module length on membrane performance through model simulation. A simulation model of pervaporative dehydration through membrane module assemble in which a number of unit modules are connected in parallel or in series has been established. In this study, ethanol/water mixture was used as model mixture. Some of permeation parameters in the model were quantified directly from the real dehydration pervaporation of ethanol through a lab-made membrane. By adopting the coefficients determined empirically the simulation model could be of more practical value. The simulation of pervaporation with two basic module configurations, that is, parallel connection and series connection, could present the importance of process parameters such as feed rate, module connection mode, number of stages, and inter-stage heating.

• Journal of ILASS-Korea
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• v.13 no.4
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• pp.212-219
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• 2008

To develop the twin-fluid atomizer having the excellent performance of painting, the spray characteristics of how a wide area can be painted efficiently by one time spraying were studied in this paper. Spray phenomena are affected by the many factors determining the spray field such as the spraying pressure of gas, the spraying pressure and viscosity of liquid paints, the opening duration of needle valve, the design dimension of nozzle, and so on. As the results of experiments, these factors affecting on spray characteristics were suggested as followings; 1) The optimum spraying pressure of gas was $0.015{\sim}0.02\;kPa$, and the appropriate spraying pressure of liquid paint was 0.01kPa, In these situations, the setting up pressures must be compensated as much as the losing amount of pressure because a decompression occurred when operating valves. 2) The duration of opening the needle valve must be sustained for $1{\sim}2$ seconds to inject gas after spraying the liquid paint. This operating of the needle valve was necessary to avoid the affect on the changing of liquid column length, and to prevent the droplet deposit at the initial time of spraying. 3) The spray tip penetration was gained form the experimental equation, and the effective spraying angle was $85^{\circ}{\pm}5^{\circ}$ just at he appropriate spraying pressure of gas. The distribution of the area sprayed had the variation in $350{\pm}50\;mm$ because of the spraying pressure of gas, the its distance from the spray tip, and the lift of the needle valve.

• Journal of Korean Association for Spatial Structures
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• v.2 no.3 s.5
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• pp.81-92
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• 2002

The objective of this paper is to help to make decision of the appropriate structural types in long span structured building due to range of span. For the intention, based on 7 forces of structural element, it is analized the relationships among 6 configurations of structural element(d/1), 25 structural types, 4 materials, and span-length known with 186 sample from 1850 to 1996. 1) bending forces: $club(1/100{\sim}1/10),\;plate(1/100{\sim}1/10),\;rahmen(steel,\;10{\sim}24m)\;simple\;beam(PC,\;10{\sim}35m)$ 2) shearing forces: $shell(1/100{\sim}1/1000)\;hyperbolic\;paraboloids(RC,25{\sim}97m)$ 3) shearing+bending forces: plate, folded $plate(RC21{\sim}59m)$ 4) compression axial forces: club, $arch(RC,\;32{\sim}65m)$ 5) compression+tension forces: shell, braced dome $shell(RC,\;40{\sim}201m),\;vault\;shell(RC,\;16{\sim}103m)$ 6) compression+tension axial forces: $rod(1/1000{\sim}1/100)$, cable(below 1/1000)+rod, coble+rod+membrane(below 1/1000), planar $truss(steel,\;31{\sim}134m),\;arch\;truss(31{\sim}135m),\;horizontal\;spaceframe(29{\sim}10\;8m),\;portal\;frame(39{\sim}55m),\;domical\;space\;truss(44{\sim}222m),\;framed\;\;membrane(45{\sim}110m),\;hybrid\;\;membrane\;(42{\sim}256m)$ 7) tension forces: cable, membrane, $suspension(60{\sim}150m),\;cable\;\;beam(40{\sim}130m),\;tensile\;membrane(42{\sim}136m),\;cable\;-slayed(25{\sim}90m),\;suspension\;membrane(24{\sim}97m),\;single\;layer\;pneumatic\;structure(45{\sim}231m),\;double\;layer\;pneumatic\;structures(30{\sim}44m)$

• Journal of Broadcast Engineering
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• v.19 no.3
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• pp.296-306
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• 2014

This paper addresses a problem of non-dialog section detection for the DVS authoring, the goal of which is to find meaningful section from the broadcasting audio, where audio description can be inserted. The broadcasting audio involves the presence of various sounds so that it first discriminates between speech and non-speech for each audio frame. Proposed method jointly exploits the inter-channels structure and speech source characteristics of the broadcasting audio whose number of channel is stereo. Also, rule based post-processing is finally applied to detect the non-dialog section whose length is appropriate for audio description. Proposed method provides more accurate detection compared to conventional method. Experimental results on real broadcasting contents show that qualitative superiority of the proposed method.

• BMB Reports
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• v.38 no.5
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• pp.602-608
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• 2005

As a crucial transcription factor family, heat-shock factors were mainly analyzed and characterized in tomato and Arabidopsis. In this study, we isolated two putative heat shock factors OsHSF6 and OsHSF12 that interact specifically with heat-shock element (HSE) from Oryza sativa L by yeast one-hybrid method. The full-length cDNA of OsHSF6 and OsHSF12 have 1074bp and 920bp open reading frame (ORF), respectively. Analysis of the deduced amino acid sequences revealed that OsHSF6 was a class A heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor, while OsHSF12 was a class B HSF with C-terminal domain (CTD) lacking of AHA motif. Bioinformatic analysis showed that the sequences and structures of two HSFs' DNA binding domain (DBD) had a high similarity with LpHSF24. The results of RT-PCR indicated OsHSF6 gene was expressed immediately after rice plants exposure to heat stress, and the transcription of OsHSF6 gene accumulated primarily in immature seeds, roots and leaves. However, we did not find the transcription of OsHSF12 gene in different organs and growth periods. Our results implied that OsHSF6 might be function as a HSF regulating early expression of stress genes in response to heat shock, and OsHSF12 might be act as a synergistic factor to regulate the expression of down-stream genes.

• The Journal of the Korea Contents Association
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• v.10 no.2
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• pp.146-154
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• 2010

Argument on the postmodernism of philosophy and history, epic and effect, and culture and art is becoming more ambiguous in scope due to digital technology development. Digital technology makes to change of aesthetic approach over the length and breadth of art and culture including medium art. Analysis of Digital images is changed from presence of images to feature analysis of images which is made by digital technology, and raise the question of how to analyze the images of reproducing digital technology focused on realism. Digital image, focused on hyper reality, should be approached not by film aesthetic approach but by shot unit. Due to emphasizes image reality reproduction by frame unit and not the short unit of the narrative approach, the artistic approach should differ from previous realism practice. Particularly, when considering movies which is focused on illusion, the realistic approach should be realized not in the of realistic approach in narrative aspect but in the aspect of realistic approach in painting aspect.

• The Journal of the Korea Contents Association
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• v.8 no.4
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• pp.273-284
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• 2008

Movements are a critical ability to young children's whole development, including physical, social/emotional, and cognitive development. This paper proposes the method to estimate movements suitable for young children's body conditions. The proposed method extracts a silhouette in each frame of videos that are obtained by deploying two video cameras by compensating illuminations, removing background and conducting morphology operations. And we extract silhouette feature values: an area, the ratio of length to width, the lowest foot position, and 7 Hu moments. Also, the area and movements of sub-area are used as local features. For motion estimation, we used probability propagation of the features extracted from the front and side frames. The proposed estimation algorithm is demonstrated for seven movements, walking, jumping, hopping, bending, stretching, balancing, and turning.