• Clinics in Shoulder and Elbow
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• 제15권1호
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• pp.37-42
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• 2012

목적: 총상에 의한 상완골 골절 증례를 통해 치료의 원칙에 대하여 고찰한다. 대상 및 방법: 28세 남자가 좌측 상완부의 총상으로 수상 후 5일에 응급실로 내원하였다. 상지의 신경 및 혈관 손상의 징후 및 타장기의 손상은 없었으며, 생체징후는 안정적이었다. 수상 당시 타 병원에서 총상에 대한 변연 절제술 및 세척술 후에 일차적 봉합술을 시행받았으며, 상완부의 전외측에 5 cm 가량의 오염된 상처가 있었다. 본원에서 변연절제술 및 탄환 파편의 제거술을 시행하고 외고정을 통하여 해부학적 정렬을 유지하였다. 술 후 8일까지 상처에 대한 무균적 소독을 시행하였으며, 정맥 항생제를 유지하였다. 술 후 9일째에 광범위 변연 절제술 및 항생제를 섞은 시멘트 구슬 삽입술을 시행하였다. 감염이 호전되는 소견을 보여, 시멘트 구슬 삽입 2주째에 외고정 장치를 제거하고 외고정 핀 삽입부의 피부 봉합술을 시행하였다. 1주 후에, 금속판을 이용하여 내고정을 하였다. 결과: 유합술 후 3 개월째, 만족스러운 상완골의 정렬과 골 유합을 얻었다.

• Clinics in Shoulder and Elbow
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• 제21권3호
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• pp.151-157
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• 2018

Background: Nonunion of lateral humeral condyle fracture causes cubitus valgus deformity. Although corrective osteotomy or osteosynthesis can be considered, there are controversies regarding its treatment. To evaluate elbow joint biomechanics in non-united lateral humeral condyle fractures, we analyzed the motion of elbow joint and pseudo-joint via in vivo three-dimensional (3D) kinematics, using 3D images obtained by computed tomography (CT) scan. Methods: Eight non-united lateral humeral condyle fractures with cubitus valgus and 8 normal elbows were evaluated in this study. CT scan was performed at 3 different elbow positions (full flexion, $90^{\circ}$ flexion and full extension). With bone surface model, 3D elbow motion was reconstructed. We calculated the axis of rotation in both the normal and non-united joints, as well as the rotational movement of the ulno-humeral joint and pseudo-joint of non-united lateral condyle in 3D space from full extension to full flexion. Results: Ulno-humeral joint moved to the varus on the coronal plane during flexion, $25.45^{\circ}$ in the non-united cubitus valgus group and $-2.03^{\circ}$ in normal group, with statistically significant difference. Moreover, it moved to rotate externally on the axial plane $-26.75^{\circ}$ in the non-united cubitus valgus group and $-3.09^{\circ}$ in the normal group, with statistical significance. Movement of the pseudo-joint of fragment of lateral condyle showed irregular pattern. Conclusions: The non-united cubitus valgus group moved to the varus with external rotation during elbow flexion. The pseudo-joint showed a diverse and irregular motion. In vivo 3D motion analysis for the non-united cubitus valgus could be helpful to evaluate its kinematics.

• The Plant Pathology Journal
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• 제16권5호
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• pp.269-279
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• 2000

Alfalfa mosaic alfamoviruses(AIMV) were isolated from infected potato (Solanum tuberosum) and azuki bean (Paseolus angularis) in Korea. Two AIMV isolated from potatoes were named as strain KR (AIMV-KR1 and KR2) and AIMV isolated from azuki bean was named as strain Az (AIMV-Az). Each isolated AIMV strain was characterized by using their host ranges, symptom developments, serological relations and nucleotide sequence analysis of coat protein (CP) gene. Strains KR1, KR2, and Az were readily transmitted to 20 of 22 inoculated plant species including bean, cowpea, tomato, tobacco, and potato. AIMV-KR1 and KR2 produced the typical symptoms like chlorotic or necrotic spots in Chenopodium quinoa and Solanum tuberosum cv. Superior. AIMV-Az caused bright yellow mosaic symptom and leaf malformation in Nicotiana glauca, which were different from the common mosaic symptom caused by AIMV-KR1 and KR2. Electron microscope observation of purified virus showed bacilliform virions containing a single-stranded plus-strand RNAs of 3.6, 2.6, 2.0 and 0.9 kbp in length, respectively, similar in size and appearance to those of Alfamovirus. In SDS-PAGE, the coat protein of the two viruses formed a consistent band that estimated to be about 24kDa. The CP genes of the AIMV strains, KR1, KR2, and Az have been amplified by RT-PCR using the specific primers designed to amplify CP gene from viral RNA-3, cloned and sequenced. Computer aided analysis of the amplified cDNA fragment sequence revealed the presence of a single open reading frame capable of encoding 221 amino acids. The nucleotide and peptide sequence of viral CP gene showed that strain KR1, KR2, and Az shared highest nucleotide sequence identities with AIMV strain 425-M at 97.7%, 98.2%, and 97.2%, respectively. CP gene sequences of two strains were almost identical compared with each other. Altogether, physical, serological, biological and molecular properties of the purified virus.

• Parasites, Hosts and Diseases
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• 제35권2호
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• pp.119-126
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• 1997

Accnthamoebn sp. W-4는 영양형 및 포낭의 형태학적 특징이 A. culbefson가 비슷하지만. 마우스에 대한 병원성. in vitro 세포독성. isoenzyme pattern 비교 분석 및 콩-특이성 난세포군 항체 교차반응 등에 의하변 A. culbensoni와는 조금 다르다. 많은 아메바들이 다양한 환경에서 다양한 형태로 분리됨으로써 종 농정에 있어서 좀더 다양한 정보를 얻고자 분자유전학적 접근을 시도하였다 본 실험은 Acanthcmoebc sp. YM-4(한국 분리수)에 대한 미토콘드리아 DNA(mtDNA)를 분리하여. 여러 종의 제한효소를 처리함으로써 mtDNA의 단편들을 얻은 다 전체 크기 및 제한효소 절단 단편길이 다형성(RFLP) 분석을 하였다. 5가지의 제한효소 즉 Hce III. Hind III . Cla I. pvu II 빛 Sal I으로 처리된 Acanthamoebn의 mtDNA는 최소 3개의 단편들고부터 많은 것은 15개의 단편들로까지 나뉘어졌다. 단편들을 합산한 mtDNA의 전체 크기는 Acnnthcmoebc sp. YM-4가 평균 46.4 kbp였으며 A. culbertsoni 및 A. potwphcgc는 각각 48.3 kbp 및 48.8 kbp로 관찰되었다 제한효소 단편길이들은 0. 6 kip초부터 34. 4 kbp가시 다양하였으며. Accnthcmoebc sp. YM- 4의 mtDNA 단편들을 A. culbertsorli 및 A. polyphnbc와 비교해 볼 때 각각 총 67개 및 65개 중에서 총통으로 갖은 단편들이 각각 9개 및 7개로 관찰되었다. 그것을 토대로 genetic divergence를 계산한 결과 Accnthnmoebn sp. YM-4차 A. culbertsoni 간에는 10.1%였으며 A. poIWphogc와는 0.99%였다. 이런 다형성의 결과는 Acnnthcmoebc sp. YM-4가 A. culbertsoni 및 A. poIMphafc와는 종이 다를 수 있다는 것을 보여준다고 하겠다.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.737-743
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• 2015

In order to examine the prevalence of Cryptosporidium infection in wild rodents and insectivores of South Korea and to assess their potential role as a source of human cryptosporidiosis, a total of 199 wild rodents and insectivore specimens were collected from 10 regions of South Korea and screened for Cryptosporidium infection over a period of 2 years (2012-2013). A nested-PCR amplification of Cryptosporidium oocyst wall protein (COWP) gene fragment revealed an overall prevalence of 34.2% (68/199). The sequence analysis of 18S rRNA gene locus of Cryptosporidium was performed from the fecal and cecum samples that tested positive by COWP amplification PCR. As a result, we identified 4 species/genotypes; chipmunk genotype I, cervine genotype I, C. muris, and a new genotype which is closely related to the bear genotype. The new genotype isolated from 12 Apodemus agrarius and 2 Apodemus chejuensis was not previously identified as known species or genotype, and therefore, it is supposed to be a novel genotype. In addition, the host spectrum of Cryptosporidium was extended to A. agrarius and Crosidura lasiura, which had not been reported before. In this study, we found that the Korean wild rodents and insectivores were infected with various Cryptosporidium spp. with large intra-genotypic variationa, indicating that they may function as potential reservoirs transmitting zoonotic Cryptosporidium to livestock and humans.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.473-480
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• 2017

Plasmodium vivax merozoite surface protein-1 (PvMSP1) gene codes for a major malaria vaccine candidate antigen. However, its polymorphic nature represents an obstacle to the design of a protective vaccine. In this study, we analyzed the genetic polymorphism and natural selection of the C-terminal 42 kDa fragment within PvMSP1 gene ($PvMSP1_{42}$) from 77 P. vivax isolates, collected from imported cases of China-Myanmar border (CMB) areas in Yunnan province and the inland cases from Anhui, Yunnan, and Zhejiang province in China during 2009-2012. Totally, 41 haplotypes were identified and 30 of them were new haplotypes. The differences between the rates of non-synonymous and synonymous mutations suggest that $PvMSP1_{42}$ has evolved under natural selection, and a high selective pressure preferentially acted on regions identified of $PvMSP1_{33}$. Our results also demonstrated that $PvMSP1_{42}$ of P. vivax isolates collected on China-Myanmar border areas display higher genetic polymorphisms than those collected from inland of China. Such results have significant implications for understanding the dynamic of the P. vivax population and may be useful information towards China malaria elimination campaign strategies.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.157-163
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• 2016

본 연구는 국내 동, 서 및 남해안 바닷가 근처 사구지역에서 자생하는 번행초 55개체를 수집하여 AFLP 마커시스템을 적용하고 이들 유전자원들간의 유전다양성 차이를 알아보기 위하여 실시하였다. 우선 전체 게놈의 특이적 부위를 절단하기 위하여 제한효소로 EcoRI과 MseI 12개 조합을 활용하였고, 그 결과 총 1,279 절편을 확보할 수 있었다. 이 결과는 제한효소 조합당 평균 107개의 절편이 생산된 것으로 이 중 평균 62개(약 58%)가 유전자원간에 다형성을 나타냈다. 이와 같이 유전자원간에 다형성을 보인 게놈 절편을 대상으로 유전다양성을 분석한 결과 조사된 55개체 번행초 유전자원 집단은 29%의 유전적 차이를 보이는 것을 알 수 있었다. 또한 군집분석을 통해 유전적 차이를 보이는 그룹을 분류한 결과 국내 자생 번행초 유전자원은 총 7개의 집단으로 나누어짐을 알 수 있었다. 본 연구에서 국내외 최초의 번행초 유전 다양성 평가 정보는 향 후 품종 육성을 위한 교배친의 선발에 적용하여 다양한 유전적 차이를 보이는 분리집단을 확보하는 데 활용이 가능할 것으로 생각된다.

• 한국임상수의학회지
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• 제22권4호
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• pp.386-391
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• 2005

This study was done to produce a mutated protein inactivated cytotoxicity of Shigatoxin 2e (Stx2e) of E.coli O139 isolates by deletional mutagenesis of Stx2e A subunit gene encoding active-site cleft of enzymatic domain in ST2e holotoxin. Cytotoxicity of the toxoid expressed from the mutant Stx2e gene was compared with wild type Stx2e for development of vaccine candidate. A recombinant plasmid pED18 containing Stx2e gene ot E.coli O139 isolates was used to generate mutation plasmid. Deletion mutagenesis was conducted for Stx2e A subunit gene encoding enzymatically active domain by polymerase chain reaction (PCR) using ot designed primer to induce deletional mutation. DNA sequence analysis was confirmed that the pentamer (Typ 202- Ser 206) that lies within the proposed active-site cleft in the second region was completely deleted. A DNA fragment of 1.1 kb that encode the new mutant Stx2eA gene was inserted into plasmid pRSET vector digested with EcoRV-Hind III and named pEDSET The PEDSET was transformed in E. coli for expression of mutant protein and the protein was confirmed by SDS-PACE and Western-blotting. The protein expressed by the mutant was tested to confirm the reduction of cytotoxic activities on Vero cell using microcytotoxicity assay compared with wild type Stx2e, the cytotoxicity of deletional mutant protein was at least reduced by 3,000-fold on Vero cell.

• 한국임상수의학회지
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• 제32권1호
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• pp.5-8
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• 2015

D-dimer란 섬유소분해산물로 응고혈액이 섬유소용해 후에 혈액 내에 보이는 작은 단백질 파편이다. D-dimer의 농도는 혈전증과 혈전색전증을 결정하기 위해서 널리 사용되고 있다. 인의에서 혈전색전증의 주요 원인 중 하나는 울혈성 심부전이기 때문에, 금번 연구에서 만성 이첨판 폐쇄부전증에 의한 울혈성 심부전의 다양한 심각도를 가진 개들에서 혈전색전증의 정도와 위험성을 조사하였다. 혈장 d-dimer의 농도는 건강한 개 20마리와 만성 이첨판 폐쇄부전증에 의해 울혈성 심부전에 이환된 다양한 중등도의 30마리 개에서 평가되었다. D-dimer의 농도는 상품화 된 키트로 측정하였다. 혈장 D-dimer의 농도는 건강한 개체 집단과 만성 이첨판 폐쇄부전증에 이환된 집단 사이에 유의적인 차이는 존재하지 않았다. 게다가, d-dimer의 농도는 심초음파 인덱스 중 대동맥대 좌심방비, 대동맥대 좌심실 이완말기 직경비와 연관성이 보이지 않았고, 이번 연구 집단의 심부전의 심각도와도 연관성이 존재하지 않았다. 따라서 금번 연구는 심부전을 가진 개에서 혈전색전증의 정도가 심하지 않거나 혈장 d-dimer의 농도 검사 자체가 개의 혈전색전증을 발견하는데 신뢰하지 못하다는 점을 암시하고 있다.

• 대한미생물학회지
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• 제35권3호
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• pp.263-271
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• 2000

A clinical isolate of Klebsiella pneumoniae K7746 produced the extended-spectrum ${\beta}$-lactamase (ESBL) SHV-12. A 6.6 kb BamHI fragment containing the $bla_{SHV-12}$ gene of K7746 strain was cloned into pCRScriptCAM vector resulting in the recombinant plasmid p7746-Cl. The restriction map of 3.6 kb inserted DNA and sequences immediately surrounding $bla_{SHV-12}$ of p7746-C1 were homologous to plasmid pMPA2a carrying $bla_{SHV-2a}$. In addition, both $bla_{SHV-12}$ and $bla_{SHV-2a}$ were expressed from a common hybrid promoter made of the -35 region derived from the left inverted repeat of IS26 and the -10 region from the $bla_{SHV}$ promoter itself. The results indicate that $bla_{SHV-12}$ and $bla_{SHV-2a}$ may have evolved from a common ancestor in the sequential order of $bla_{SHV-2a}$ first, followed by $bla_{SHV-12}$. Furthermore, by the PCR mapping method using primers corresponding to the IS26 and $bla_{SHV}$, the association between IS26 and $bla_{SHV}$ was studied in 12 clinical isolates carrying $bla_{SHV-2a}$, 27 clinical isolates carrying $bla_{SHV-12}$, and 5 reference strains carrying $bla_{SHV-1}$ to $bla_{SHV-5}$. All 39 strains carrying $bla_{SHV-2a}$ or $bla_{SHV-12}$ were positive by the PCR, providing confirmative evidence that IS26 has been involved in the evolution and dissemination of $bla_{SHV-2a}$ and $bla_{SHV-12}$. But 5 reference strains carrying $bla_{SHV-1}$ to $bla_{SHV-5}$ were negative by the PCR. Therefore, we concluded that the molecular evolutionary pathway of $bla_{SHV-2a}$ and $bla_{SHV-12}$ may be different from that of other $bla_{SHV-ESBL}$, e.g., $bla_{SHV-2}$, $bla_{SHV-3}$, $bla_{SHV-4}$, and $bla_{SHV-5}$.