• Title/Summary/Keyword: fractionation.

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Separation and Characterization of Dust and Ground Water Particulates Using Gravitational SPLITT Fractionation.

  • Lee, Seung Ho;Park, Hui Yeong;Lee, Sang Geun;Yong, Seong Gwon;Eum, Cheol Hun
    • Bulletin of the Korean Chemical Society
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    • v.22 no.6
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    • pp.616-622
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    • 2001
  • Split-flow thin (SPLITT) cell Fractionation(SF) is a technique that allows separation of particulates and macromolecules into two fractions. A gravitational SF(GSF) system is constructed and tested for its applicability for separation of dust and ground water particulates. When tested with polystyrene latex particles, experimental data were in good agreements with theory. The 9.8 and 21.4㎛ polystyrene particles were successuflly separated in a continuous mode, where the mixture is continuously fed into the GSF channel allowing separation in a large sacle. The GSF system is successfully applied to continuous separation of dust and ground water particels based on the sedimentation coefficient, which is closely related to the particle size. The separations were confirmed by microscopy and energy-dispersive X-ray (EDX) analysos.

Optimization of Acetone-Fractionation for 1-Palmitoyl-2-Oleoyl-3-Oleoyl Glycerol and 1-Palmitoyl-2-Oleoyl-3-Palmitoyl Glycerol by Response Surface Methodology (반응표면분석법에 의한 1-Palmitoyl-2-Oleoyl-3-Oleoyl Glycerol과 1-Palmitoyl-2-Oleoyl-3-Palmitoyl Glycerol의 아세톤 분별 공정 최적화)

  • Shin, Jung-Ah;Sung, Min-Hye;Lee, Sun-Mo;Son, Jeoung-Mae;Lee, Jeung-Hee;Hong, Soon-Taek;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.975-980
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    • 2011
  • 1-Palmitoyl-2-oleoyl-3-oleoyl glycerol (POO) and 1-palmitoyl-2-oleoyl-3-palmitoyl glycerol (POP) were enriched from palm stearin using an acetone fractionation process. Response surface methodology was employed to optimize the purity of POO ($Y_1$, %) and POP ($Y_2$, %) along with POO+POP content ($Y_3$, g) based on independent variables such as fractionation temperature ($X_1$, 25, 30, and $35^{\circ}C$) and the ratio of palm stearin to acetone ($X_2$, 1:3, 1:6 and 1:9, w/v). Fractionation conditions were optimized to maximize $Y_1$, $Y_2$, and $Y_3$, in which fractionation temperature was $29.3^{\circ}C$ with a 1:5.7 acetone ratio. With such parameters, 60.9% of POP and 23.8% of POO purity were expected with a 75% yield (3.0 g) of POO and POP.

Rayleigh Fractionation of Stable Water Isotopes during Equilibrium Freezing (평형 냉동에 의한 물동위원소의 레일리분별)

  • Lee, Jeonghoon;Jung, Hyejung;Nyamgerel, Yalalt
    • Economic and Environmental Geology
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    • v.54 no.1
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    • pp.61-67
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    • 2021
  • Isotopic compositions of snow or ice have been used to reconstruct paleoclimate and to calculate contribution to streamwater using isotopic hydrograph separation as an end member. During freezing and melting of snow or ice, isotopic fractionation occurs between snow or ice and liquid water. Isotopic evolution during melting process has been studied by field, melting experiments and modeling works, but that during freezing has not been well studied. In this review, isotopic fractionation during equilibrium freezing is discussed using the linear relationship between two stable water isotopes (oxygen and hydrogen) and the Rayleigh fractionation. Snow, evaporated from nearby ocean and condensated, follows the Global Meteoric Water Line (slope of 8), but the melting and freezing of snow affect the linear relationship (slope of 19.5/3.1~6.3). The isotopic evolution of liquid water by freezing observed in the open system during Rayleigh fractionation is also seen in the closed system. The isotopic evolution of snow or ice in the open system where the snow or ice is continuously removed becomes more enriched than the residual liquid water by the fractionation factor. The isotopic evolution of snow or ice in the closed system eventually equals the original isotopic compositions of liquid water. It is expected the understanding of isotopic evolution of snow or ice by freezing to increase the accuracy of the paleoclimate studies and hydrograph separation.

Evaluation of the COD Fractionation Capability Using Storage Microorganism from EBPR Process (EBPR 공정내 저장 미생물을 이용한 유입수 분율 분석능 평가)

  • Kim, Youn-Kwon;Seo, In-Seok;Kim, Hong-Suck;Kim, Ji-Yeon
    • Journal of the Korean GEO-environmental Society
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    • v.5 no.4
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    • pp.25-31
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    • 2004
  • In conventional activated sludge process, COD fractions in wastewater are important parameters, significantly. Depending on characteristics of influent COD fractionation, activated sludge process requires a major change of a process operation to ensure meeting a stricter standards. In order to validate and evaluate the accuracy of the traditional COD fractionation methodologies, readily and slowly biodegradable COD was mixed using glucose and peptone as a sole carbon source in a synthetic wastewater, respectively. In this research, prediction of the COD fraction was made using the OUR(Oxygen Utilization Rate) and the NUR(Nitrate Utilization Rate) experiments. The result showed that COD fractions calculated by OUR experiment were similar to the composition of synthetic wastewater. On the other hand, it was found that an error was generated during the NUR experiment. This error was due to the intracellular storage period for storage microorganisms such as PAOs, and the error in COD fraction was observed about 8-14 % in terms of Total COD.

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Stereotactic radiotherapy of the prostate: fractionation and utilization in the United States

  • Weiner, Joseph P.;Schwartz, David;Shao, Meng;Osborn, Virginia;Choi, Kwang;Schreiber, David
    • Radiation Oncology Journal
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    • v.35 no.2
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    • pp.137-143
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    • 2017
  • Purpose: To analyze the utilization and fractionation of extreme hypofractionation via stereotactic body radiotherapy (SBRT) in the treatment of prostate cancer. Materials and Methods: Data was analyzed on men diagnosed with localized prostate cancer between 2004-2012 and treated with definitive-intent radiation therapy, as captured in the National Cancer Database. This database is a hospital-based registry that collects an estimated 70% of all diagnosed malignancies in the United States. Results: There were 299,186 patients identified, of which 4,962 (1.7%) were identified as receiving SBRT as primary treatment. Of those men, 2,082 had low risk disease (42.0%), 2,201 had intermediate risk disease (44.4%), and 679 had high risk disease (13.7%). The relative utilization of SBRT increased from 0.1% in 2004 to 4.0% in 2012. Initially SBRT was more commonly used in academic programs, though as time progressed there was a shift to favor an increased absolute number of men treated in the community setting. Delivery of five separate treatments was the most commonly utilized fractionation pattern, with 4,635 patients (91.3%) receiving this number of treatments. The most common dosing pattern was $725cGy{\times}5fractions$ (49.6%) followed by $700cGy{\times}5fractions$ (21.3%). Conclusions: Extreme hypofractionation via SBRT is slowly increasing acceptance. Currently $700-725cGy{\times}5fractions$ appears to be the most commonly employed scheme. As further long-term data regarding the safety and efficacy emerges, the relative utilization of this modality is expected to continue to increase.

Recovery and Fractionation of Serine Protease Inhibitors from Bastard Halibut Paralichthys olivaceus Roe (넙치(Paralichthys olivaceus) 알로부터 Serine Protease Inhibitors의 분획 특성)

  • Kim, Hyung Jun;Lee, Hyun Ji;Park, Sung Hwan;Jeon, You-Jin;Kim, Jin-Soo;Heu, Min Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.2
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    • pp.178-186
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    • 2015
  • Protease inhibitors (PI) of trypsin and papain as target proteases from the roe of bastard halibut Paralichthys olivaceus were fractionated out using ammonium sulfate precipitation (A), DEAE 650M anion exchange chromatography (D), and Sephacryl S-300 gel filtration (S). The recovery percentages of the fractions with the strongest inhibitory activity for each fractionation method were 13% for the A4 fraction, 21.2% for the D3 fraction, and 21.3% for the S2 fraction, with specific inhibitory activities of the fractions toward trypsin and casein of 168, 139, and 218 U/mg, respectively, while no inhibition of papain was observed. The $IC_{50}$ for the trypsin-specific substrate $N{\alpha}$-benzoyl-$\small{L}$-arginine-p-nitroanilide (BAPNA) was 0.65, 1.55, 2.26, and 2.85 mg/mL for the A4, S2, A3, and D3 fractions, respectively. These results suggest that chromatographic fractionation methods (D and S) based on the molecular mass and charge of the protein were more effective at fractionating PI than was ammonium sulfate precipitation based on protein solubility, and that the bastard halibut roe extract acts as a serine protease inhibitor. Therefore, the PI fraction from fish roe might be useful for inhibiting proteases in foodstuffs, and could constitute an alternative food-grade inhibitor for the surimi industry.