• Title/Summary/Keyword: fraction as division

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Characterization of Anti-complementary Polysaccharide from Teucrium viscidum var. miquelianum

  • Min, Jin-Gi;Kim, Tae-Jin;Lee, Doo-Seog;Ch, Sang-Won;Yoon, Ho-Dong;Park, Jeong-Heum
    • Preventive Nutrition and Food Science
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    • v.6 no.3
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    • pp.137-141
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    • 2001
  • Water-soluble crude polysaccharide (TM-1) prepared from the leaves of Teucrium viscidum var. miquelianum was fractionated into three polysaccharide fractions, TM-2, TM-3 and TM-4 by the addition of cetyltrimethylammonium bromide. The major polysaccharide fraction, TM-2, consisted of glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, and all fractions contained galactose and glutose as the major neutral sugars. TM-4 showed the highest anti-complementary activity. When TM-4 was futher fractionated by anion exchange chromatography, TM4- II a and TM4-II b showed the most Potent anti-complementary activity. TM4- II a was composed mainly of galactose, arabinose and glucose in the molar ratios of 2.13 : 0.94 : 1.00 respectively, and contained a small amount of galacturonic acid and glucuronic acid.

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Hydrogen Bonding Effect on γ-Ray Irradiated Poly(vinyl alcohol) Hydrogels in Different Drying Conditions

  • Gwon, Hui-Jeong;Jo, Sun Young;Park, Eun Ji;Shin, Young Min;Choi, Jong-Bae;Park, Jong-Seok;Lim, Youn-Mook;Nho, Young-Chang;Kang, Phil Hyun
    • Journal of Radiation Industry
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    • v.6 no.1
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    • pp.89-94
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    • 2012
  • Three-dimensional network hydrogels were prepared by ${\gamma}$-irradiation of aqueous solutions of poly(vinyl alcohol) (PVA) and glycerol (Gly). Oven-drying was used to measure the gel fraction (G), hydration (H) or swelling behavior (S) of the prepared hydrogels. This study made a hypothesis that hydrogen bonds due to addition of glycerol and change of dry states such as freeze-drying (FD), room-drying (RD) and oven-drying (OD) acts on the G, H, and S. Interesting results on the hydrogen bonding effect in the prepared hydrogels are monitored at different drying conditions. The FD samples have a higher G values with increase in glycerol content as compared with the OD and RD samples. The formation of strong hydrogen bonding network among Gly molecules and hydrogel matrix was considered as the main driving force, resulting in the changes in the G, H, and S of the hydrogels under different drying conditions.

Antioxidative Effects and Anti-proliferative Effects of MeOH, BuOH and Ethyl Acetate Fractionated from Stephania delavayi Diels (일문전(Stephania delavayi Diels.) 메탄올, 부탄올, 에틸아세테이트 분획물의 항산화 및 세포증식억제 효과)

  • Li, Yong-Chun;Kim, Kyoung-Hee;Xu, Hong-De;Park, Dae-Hun;Choi, Yeon-Shik;Hwang, Hye-Rim;Lee, Min-Jae;Choi, Jong-Jin;Kwon, Myung-Sang;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.297-301
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    • 2009
  • Stephania delavayi Diels. (S. delavayi Diels.) has been used as a drug for pain-relieving and acute gastroenteritis treatment in China. Because the major therapeutic mechanism of anti-inflammatory drug is to inhibit the cyclooxygenase (COX)-2 and because COX-2 proteins inhibit apoptosis, COX-2 inhibitor has been thought as the anticancer drug candidate. For this reason, we examined S. delavayi Diels. as an anticancer drug. S. delavayi Diels. was fractionated with methanol and then partitioned with ethyl acetate, n-butanol and water. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power. DPPH radical scavenging activities of the crude fractions at the concentration of $1,000{\mu}g/mL$ were 75.23% (n-butanol), 68.11% (methanol), 63.58% (ethyl acetate), and 50.13% (water). The reducing power increased according to the concentration in dose-dependent manner. Also, when the antiproliferation effects of each fraction against human breast cancer cell-lines MDA-MB-231 and MCF-7 were examined, methanol extract, n-butanol fraction and ethyl acetate fraction exhibited cell proliferative inhibition effects in both cell-lines whereas water fraction did not. Among the crude fractions, the n-butanol fraction exhibited the most potent anti-proliferation effect. In conclusion, fractions from S. delavayi Diels. are promising anticancer drug candidates.

Functional and Volatile Flavor Components in Myungtae(Alaska pollack) sikhae (마른명태 식해의 향기성분과 기능성)

  • Koo, Tae-Ho;Zhang, Yun-Bin;Choi, Hee-Jin;Woo, Hi-Seoh;Son, Gyu-Mok;Choi, Cheong
    • Journal of the Korean Society of Food Culture
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    • v.17 no.5
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    • pp.535-542
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    • 2002
  • The volatile compounds of Myungtae (Alaska pollack) sikhae obtained by simultaneous steam distillation and extraction(SDE) apparatus were separated by gas chromatography(GC) and gas chromatography mass spectrometry(GC/MS). The totals of 155 volatile flavor components was identified in traditional Kyungsangdo Myungtae (Alaska pollack) sikhae, respectively. ${\alpha}$-Zingihirene(11.03%) (E)-di-2-propenyl disulfide(7.95%) ${\beta}$-cironellol(6.02%), methyl allyl disulfide(3.58%), cryptone(3.39%), camphene(3.23%), pentanol(3.21%), penadecanal(2.66%) and ${\beta}$-phellandrene(2.06%) were contained as the main compounds of Myungtae shikae. The fraction obtained from sikhae were tested for electron donating ability, angiotensin converting enzyme and xanthine oxidase inhibitory activity. There was no electron donating abilities$(SC_{50})$ of hexane and water fraction. On the other hand, the abilities of ethylacetate fraction and butanol fraction showed $310.64\;{\mu}g/mL,\;1096.49\;{\mu}g/mL$, respectively. Angiotensin converting enzyme inhibitory activities$(IC_{50})$ of ethylacetate fraction and butanol fraction were 1.623 mg/mL, 1.303 mg/mL, respectively. Xanthine oxidase inhibitory activities$(IC_{50})$ of ethylacetate fraction and butanol fraction were 3.591 mg/mL, 2.083 mg/mL, respectively.

Sub-Pixel Analysis of Hyperspectral Image Using Linear Spectral Mixing Model and Convex Geometry Concept

  • Kim, Dae-Sung;Kim, Yong-Il;Lim, Young-Jae
    • Korean Journal of Geomatics
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    • v.4 no.1
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    • pp.1-8
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    • 2004
  • In the middle-resolution remote sensing, the Ground Sampled Distance (GSD) that the detector senses and samples is generally larger than the actual size of the objects (or materials) of interest, and so several objects are embedded in a single pixel. In this case, as it is impossible to detect these objects by the conventional spatial-based image processing techniques, it has to be carried out at sub-pixel level through spectral properties. In this paper, we explain the sub-pixel analysis algorithm, also known as the Linear Spectral Mixing (LSM) model, which has been experimented using the Hyperion data. To find Endmembers used as the prior knowledge for LSM model, we applied the concept of the convex geometry on the two-dimensional scatter plot. The Atmospheric Correction and Minimum Noise Fraction techniques are presented for the pre-processing of Hyperion data. As LSM model is the simplest approach in sub-pixel analysis, the results of our experiment is not good. But we intend to say that the sub-pixel analysis shows much more information in comparison with the image classification.

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Antimicrobial Activities of (-)Epicatechin from Ulmus davidiana var. japonica Cortex

  • Lee, Gyu-Hee;Shim, Chang-Ju;Chang, Yeong-Il;Park, Seong-Hyun;Oh, Hong-Rock;Oh, Man-Jin
    • Preventive Nutrition and Food Science
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    • v.6 no.4
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    • pp.230-234
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    • 2001
  • The extract of Ulmus davidiana var. japonica cortex has known as natural anti-inflammatory substance in East Asia. For the identification of antimicrobial substance, it was extracted by using methanol and fractionated by using different organic solvents. The fraction of butanol was represented the highest antimicrobial activities. Therefore, the butanol fraction was purified and identified the chemical structure by $^1$H and $^{13}$ C-NMR spectra, FT-IR and EI/MS spectroscopies. The isolated antimicrobial substance was identified as cis-2-[3,4-dihydroxy phenyl]-3,4-dihydro-2H-1-benzopyran-3,5,7-triol, which has commonly known as (-)epicatechin. Its minimum inhibitory concentrations (MICs) against Staphylococcus aureus and Listeria monocytogenes were shown as 100 $\mu\textrm{g}$/mL, respectively.

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Grain Yield and Water Use Efficiency as Affected by Irrigation at Different Growth Stages

  • Kim, Wook-Han;Hong, Byung-Hee;Ryu, Yong-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.4
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    • pp.330-338
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    • 1999
  • Extensive research has been conducted on effects of drought stress on growth and development of soybean but information is rather restricted on the limited-irrigation system by way of precaution against a long-term drought condition in the future. The experiment for limited-irrigation was conducted in transparent vinyl shelter at Asian Vegetable Research and Development Center (AVRDC), Taiwan in 1997. Two soybean varieties, Hwangkeum and AGS292, improved in Korea and AVRDC, respectively were used for this experiment. The relationships between normalized transpiration rate (NTR) and fraction of transpirable soil water (FTSW) in both varieties were similar that the NTR was unchanged until FTSW dropped to about 0.5 or 0.6. At FTSW less than those values, NTR declined rapidly. Days required to harvest in both varieties were significantly prolonged at IR6 treatment compared to any other treatments. Daily mean transpiration rate was significantly higher at IR5 treatment, as averaged over varieties. Similarly, water use efficiency was also high at 1R5 treatment. In both varieties, seed yield was the greatest at the IR5 treatment, as compared to any other limited-irrigation treatments, due to the increased seed number and high transpirational water use efficiency. The indices of input water and seed yield for the different limited-irrigation treatments against control indicated that Hwangkeum produced 59.6% or 60.7% of seed yield using 36.1% or 44.9% of input water, as compared to control, by irrigation at only R5 or R6 stages, respectively. The AGS292 produced 56.1% of seed yield with 35.4% of input water of control, when irrigated at R5 stage. The results of this study have elucidated that the limited irrigation at R5 stage in soybean can be minimized yield loss with such small quantity of water under the environment of long-term drought stress and the expected shortage of agricultural water in the future.

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SUPPRESSION OF HYDROGEN CONSUMING BACTERIA IN ANAEROBIC HYDROGEN FERMENTATION

  • Park, Woo-Shin;Jang, Nam-J.;Hyun, Seung-H.;Kim, In-S.
    • Environmental Engineering Research
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    • v.10 no.4
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    • pp.181-190
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    • 2005
  • Severe loss or hydrogen occurred in most anaerobic hydrogen fermentation reactors. Several selected methods were applied to suppress the consumption of hydrogen and increase the potential of production. As the first trial, pH shock was applied. The pH of reactor was dropped nearly to 3.0 by stopping alkalinity supply and on]y feeding glucose (5 g/L-d). As the pH was increase to $4.8{\pm}0.2,$ the degradation pathway was derived to solventogenesis resulting in disappearance of hydrogen in the headspace. In the aspect of bacterial community, methanogens weren't detected after 22 and 35 day, respectively. Even though, however, there was no methanogenic bacterium detected with fluorescence in-situ hybridization (FISH) method, hydrogen loss still occurred in the reactor showing a continuous increase of acetate when the pH was increased to $5.5{\pm}0.2$. This result was suggesting the possibility of the survival of spore fanning acetogenic bacteria enduring the severely acidic pH. As an alternative and additive method, nitrate was added in a batch experiment. It resulted in the increase of maximum hydrogen fraction from 29 (blank) to 61 % $(500\;mg\;NO_3/L)$. However, unfortunately, the loss of hydrogen occurred right after the depletion of nitrate by denitrification. In order to prevent the loss entangled with acetate formation, $CO_2$ scavenging in the headspace was applied to the hydrogen fermentation with heat-treated sludge since it was the primer of acetogenesis. As the $CO_2$ scavenging was applied, the maximum fraction of hydrogen was enhanced from 68 % to 87 %. And the loss of hydrogen could be protected effectively.

New method for sclerotial isolation of Sclerotium spp. from infested soil

  • Kim, Yong-Ki;Kwon, Mi-Kyung;Shim, Hong-Sik;Yeh, Wan-Hae
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.120.1-120
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    • 2003
  • White rot on Allium species recently had a high incidence as increased cultivating areas of tropical garlic types in Korea. Two types of Sclerotium have been known as causal agents producing different size and shapes of sclerotia in infested fields. We developed a new method for isolation of two types of sclerotia from infested field soils that can be used for ecological study of sclerotium spp. and establishment of control strategy. Soil samples collected from heavily infested fields were evenly mixed and placed on a automatic sieve shaker connected with tap water. After 10 min. of shaking, residues on 0.5mm and 0.25mm sieve were separately collected and suspended with 70% sugar solution, which method floats sclerotia in aqueous layer. Then, floated fraction was carefully separated and mixed with a same volume of 1% sodium hypochlorite solution to differentiate with organic materials. This method provides direct count of sclerotia under dissecting microscopy.

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Estimation of Nonlinear Parameter in Water - saturated Sandy Sediment by using Difference Frequency Acoustic wave (수중 모래 퇴적물에서 차 주파수 음파를 이용한 비선형 변수 추정)

  • Kim Byoung-Nam;Lee Kang Il;Yoon Suk Wang;Choi Bok Kyung
    • Proceedings of the Acoustical Society of Korea Conference
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    • autumn
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    • pp.429-432
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    • 2004
  • Nonlinear acoustic responses of water-saturated sediments are very important to understand nonlinear phenomena of gassy ocean sediments. Especially, the second harmonic, the sum and the difference frequency acoustic waves in water-saturated sediments can provide practical criteria to estimate the nonlinear parameter of gassy sediments. In this paper, the difference frequency acoustic wave in water-saturated sandy sediment was observed in a water tank experiment with a pulse transmission technique. Its pressure level was 12 dB higher than the background noise level at a maximum undistorted driving pressure of source acoustic transducer. The experimental results were compared with a theoretical estimation of the parametric acoustic array. The nonlinear parameter of water-saturated sandy sediment was also estimated as 73 with their comparison. This value can be utilized as the background information to estimate gas void fraction in the water-saturated gassy sandy sediment.

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