• Animal cells and systems
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• v.6 no.4
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• pp.305-309
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• 2002

Actinorhodin antibiotics produced by Streptomyces lividans TK24 are blue pigments with a weak antibiotic activity, derived from one acetyl-CoA and 15 malonyl-CoA units via a typical ployketide pathway. In an attempt to clone polyketide biosynthetic genes of S. lividans TK24, hybridizing fragments in the genomic DNA of S. lividans TK24 were detected by use of acn and act III polyketide synthase gene probes. Since typical aromatic polyketide bio-synthetic gene clusters are roughly 22-34 Kb long, we constructed in E. coli XL-Blue MR using the Streptomyces-E. coli bifunctional shuttle cosmid vector (pojn46). Then, about 5,000 individual E. coii colonies were thor-oughly screened with acrl-ORFI and actIII probes. From these cosmid libra-ries, 12 positive clones were identified. Restriction analysis and southern hybridization showed two polyketide biosynthetic gene clusters in this organism. These cosmid clones can be transformed into Streptomyces parvulus 12434 for expression test that identify product of actinorhodin biosynthetic genes by heterologous expression. Thus, heterologous expres-sion of a derivative compound of a actinorhodin biosynthetic intermediate was obtained in pKE2430. Expression of these compounds by the trans-formants was detected by photodiode array HPLC analysis of crude extracts.

• Speech Sciences
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• v.9 no.2
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• pp.25-47
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• 2002

Human language activity in the information society has been developing the communication system between humans and machines. The aim of this study was to analyze dialectal speech in Korea. One hundred Kyungsang and one hundred Cholla informants participated in this study. A CSL and Flexible laryngeal fiberscope were used for analysis of the acoustic and glottal gestures of all the vowels and consonants. Test words were made on the picture cards and letter cards which contained each vowel and each consonant, respectively. The dialogue between the examiner and the informants was recorded in a question and answer manner. The acoustic results of two dialects were as follows: Kyungsang and Cholla informants showed neutralization between /e/ and /$\varepsilon$. However, the apertures of Kyungsang vowels /i, w, u, o/ were higher than those of Cholla vowels. The /wi/ and /$\varepsilon$/ of Kyungsang Diphthong vowels were shown as simple vowels /i/ and /$\varepsilon$/ in Cholla dialect. The VOT of Cholla dilaect was longer than that of Kyungsang dialect. The fricative frequence of Kyurlgsang dialect was about 1000Hz higher than that of Cholla dialect. The glottal widths on fiberscopic images showed that the consonant durations of Kyungsang and Cholla dialects were correlated all together with the acoustic duration on the spectrogram.

• Speech Sciences
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• v.7 no.4
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• pp.59-72
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• 2000

Vocal tract resonances are of paramount importance to voice sounds. Resonance frequencies determine vowel quality and the personal voice timber. The aim of this study was to make an effective diction program according to tuning formant frequencies by adjusting the vocal tract shape in professional voice users. Twelve male student singers and eleven female student singers participated in this study. The subjects repeated five simple vowels /a, e, i, o, u/ in normal speech and singing. The spoken vowels and sung vowels were measured by formant frequencies and the singer's formant frequencies using CSL and DSP Sona-Graph. Separately, Plot formants program was used to draw the vowel chart. The results were as follows. (1) Total formant frequencies of female singers were 11% higher than those of males singers in singing. (2) The F1 and F3 of sung vowels increased compared to F1 and F3 spoken vowels. However, The F2 of sung vowels decreased in comparison with F2 of spoken vowels. (3) Posterior vowel /u/ were moved anteriorly. This phenomenon seemed to be due to head voice singing training. (4) Singer's formant frequencies in student singers appeared according to the part: 2560 Hz for baritone, 2760 Hz for Tenor, 2821 Hz for Mezzo soprano and 3420 Hz for soprano.

• Phonetics and Speech Sciences
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• v.3 no.1
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• pp.23-34
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• 2011

Many Korean children have difficulty learning English vowels. The gestures inside the oral and pharyngeal cavities are hard to control when they cannot see the gestures and the target vowel system is quite different from that of Korean. This study attempts to collect children's acoustic data of twelve English vowels published by Hillenbrand et al. (1995) online and to examine the acoustic features of English vowels for phoneticians and English teachers. The author used Praat to obtain the data systematically at six equidistant timepoints over the vowel segment avoiding any obvious errors. Results show inherent acoustic properties for vowels from the children's distribution of vowel duration, f0 and intensity values. Second, children's gestures for each vowel coincide with the regression analysis of all formant values at different timepoints regardless of the vocal fold and tract difference. Third, locus points appear higher than those of American males and females. Their gestures along the timepoints display almost similar patterns. From the results the author concludes that vowel formant trajectories provide useful and important information on dynamic articulatory gestures, which may be applicable to Korean children's education and correction of English vowels. Further studies on the developmental study of vowel formants and pitch values are desirable.

• Proceedings of the KIEE Conference
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• 1993.07a
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• pp.284-286
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• 1993

Software and hardware for cochlear implant system have been developed to create a speech signal processing system which, in real-time, extracts model parameter including formants, pitch, amplitude information. The system is based on the Texas Instruments TMS320 family. In hardware, computer interface has been desisted and implemented that allows presentation of biphasic pulse stimuli to patients with the hearing handicapped. The host computer sends a stream of bytes to the parallel port. Upon receipt of the data the interface generates the appropriate burst sequence that is delivered to the patient's external transmitter coil. The coded information is interpreted by the Nucleus-22 internal receiver that delivers the pulse to the specified electrodes at the specified amplitude and pulse width.

• Proceedings of the Korea Information Processing Society Conference
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• 2011.11a
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• pp.1039-1042
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• 2011

토마토는 주위에서 흔하게 접할 수 있는 채소 중 하나이며 여러 음식의 재료로 사용된다. 토마토는 활성 산소의 생성을 억제하고 항암 효과가 좋으며, 콜레스테롤 수치를 낮추어 주어 혈관을 강화해 준다. 또한, 인체 장기 중 심장은 혈액을 순환시키는 원동력이며 순환계의 중추 역할을 한다. 따라서 본 논문에서는 대체 및 예방의학에 이용되는 중요한 요소 중 하나인 음성을 이용하여 토마토를 섭취하였을 때 음성 분석 요소인 Jitter와 2Formants Bandswidth의 변화를 분석하였다.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.12 no.1
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• pp.34-38
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• 2001

Background and Objectives : With the development of computerized systems, an objective evaluation methods of nasal speech and nasal geometry have become readily available by means of a simple, noninvasive technique. In this study, we assessed the nasality, nasal formant, nasal volume and nasal area in patients with hypertrophic rhinitis before and after turbinate surgery. Material and Method : With the nasometer, we measured nasalance, which reflects the ratio of acoustic energy output of nasal sounds from the nasal and oral cavities. With CSL 4300B, we measured nasal formants. We used acoustic rhinometer to measure nasal area and nasal volume. Postoperative changes of above factors were compared with preoperative values. Paired t-test and Pearson's correlation were used for statistical analysis. Results : The first nasal formant frequency, nasalance scores of three passages(baby, mamma and rabbit passages), minimal cross sectional area(MCA) of narrow side, nasal volume of narrow side and nasal volume of wide side had increased significantly after turbinate surgery (p <0.05). The MCA and nasal volume of narrow side and MCA of wide side showed significant correlation with nasalance score of rabbit passage and baby passage showed significant correlation with nasal volume of narrow side(p<0.05). Conclusion : There were significant increases in nasalance scores, first nasal formant frequency, MCA and nasal volume after turbinate surgery. Thus, we must consider the possibility of voice changes postoperatively in professional voice users.

• Proceedings of the Acoustical Society of Korea Conference
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• spring
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• pp.325-328
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• 2000

According to H.B.Lee(1993), standard Korean vowel qualities are as follows: in /i/, /e/, $/\epsilon/$, /a/, /o/, /w/, they have 4 qualities each other and in /er/ there are 3 qualities. The environments of 4 qualities are iong and stressed vowel in word initial, short and stressed vowel in word initial, unstressed vowel in word initial, unstressed vowel in word finial. The aim of this study is to seek and compare with H.B.Lee(1993). Conclusively I could not find on the whole any pattern of the same types of H.B.Lee(1993) in this study And especially in Fl vowel formant values of /er/and /w/, I never found any pattern of the same types of H.B.Lee(1993). Also F2 vowel formant values of $/\varepsilon/$ and /w/ do not have any kind of pattern of the same types of H.B.Lee(1993), between them, the patternize of F2 vowel formant values in /w / is especially difficult. It is the same story of Jaekang Lee(1998). But in some case, the patternize could be done. among the whole vowels, analysis environment b has the wide width on the change of the formant value. As the another result of the analysis It is to possible to make the pattern of the old male group. The old male group on the whole is analyzed to have the most low formant values and the old women group is analyzed to have the most high formants values, but in the most high formant valus there are young women group. And the formant values's rising in 2 cases of the formant value of /er/ is analyzed to have the same pattern of H.B.Lee(1993).

• Phonetics and Speech Sciences
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• v.8 no.4
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• pp.39-47
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• 2016

While acoustic studies in the past decade documented a raised /o/ by showing their lowered first formants (F1) almost overlapped with those of high back vowel /u/, no consensus has been made in terms of how this /o/-raising affects the vowels as a system in Seoul Korean. The current study aimed to investigate the age- and gender-related differences of the relative distance among the vowels to better understand the influence of this on-going sound change on the vowel system. We measured the static and dynamic spectral characteristics (F1 and F2) of the seven Korean monophthong vowels /e a ʌ o u ɨ i/ in the spontaneous speech of Seoul Corpus, and depicted the patterns of 30 individual speakers (10 speakers in each group of teens, 20s and 40s) as a function of age and gender. The static spectral examination showed low F1 values of /o/ in the spontaneous speech corpus confirming the vowel /o/ raising, and also revealed greater F2 values of /u, ɨ/ suggesting their anterior articulations. The tendencies were stronger when the speakers were younger and female. The spectral trajectories further showed that the F1 and F2 between /o/ and /u/ were differentiated throughout the vowel mid-point although the trajectories gradually merged near the vowel mid point in the older male speakers' productions. The acoustic evidence of contrast among /o, u, ɨ/ supports that the raised /o/ is not indicative of a merger with /u/ but rather implying a chain-like vowel shift in the Seoul Korean.

• MALSORI
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• no.56
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• pp.29-47
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• 2005

Investigating and comparing English vowels $/{\varepsilon}/\;and\;/{\ae}/$, and their-supposedly- corresponding vowels in Korean /ㅔ/ and /ㅐ/, this study addresses the following questions: Do Koreans pronounce/ㅔ/ and /ㅐ/ differently? Do they pronounce English $/{\varepsilon}/\;and\;/{\ae}/$ differently? And what is the relationship between the Korean vowels and the English vowels? Is the conventional correspondence (/ㅔ:${\varepsilon}/$, and /ㅐ/:${\ae)/$ appropriate? The results showed that 24 Korean male college students distinguish neither Korean /ㅔ/ and /ㅐ/ nor English $/{\varepsilon}/\;and\;/{\ae}/$, thus suggesting that their inability to distinguish the two vowels in their native tongue has an effect on their production of the English vowels. While not distinguishable within a language, Korean /ㅔ/ and /ㅐ/ still form a separate group from English $/{\varepsilon}/\;and\;/{\ae}/$. But Korean-Produced $/{\varepsilon}/\;and\;/{\ae}/$ are significantly different from American-produced $/{\varepsilon}/\;and\;/{\ae}/:$ Korean-produced $/{\varepsilon}/\;and\;/{\ae}/$ have much lower F1 and F2 than American-produced counterparts. Accordingly it is advised that, in learning English pronunciation, Korean students should be instructed to take the English vowel system as a separate system from Korean one, and thus, not to substitute Korean vowels for English vowels. And students should be provided with specific instructions on the articulatory differences between English vowels and Korean vowels. For example, Cey should be instructed to lower their jaws more for English $/{\varepsilon}/\;and\;/{\ae}/$ than for Korean /ㅔ/ and /ㅐ/.